Kleefstra syndrome (KS) is a neurodevelopmental disorder caused by mutations in the histone methyltransferase EHMT1. To study the impact of decreased EHMT1 function in human cells, we generated excitatory cortical neurons from induced pluripotent stem (iPS) cells derived from KS patients. Neuronal networks of patient-derived cells exhibit network bursting with a reduced rate, longer duration, and increased temporal irregularity compared to control networks. We show that these changes are mediated by upregulation of NMDA receptor (NMDAR) subunit 1 correlating with reduced deposition of the repressive H3K9me2 mark, the catalytic product of EHMT1, at the GRIN1 promoter. In mice EHMT1 deficiency leads to similar neuronal network impairments with increased NMDAR function. Finally, we rescue the KS patient-derived neuronal network phenotypes by pharmacological inhibition of NMDARs. Summarized, we demonstrate a direct link between EHMT1 deficiency and NMDAR hyperfunction in human neurons, providing a potential basis for more targeted therapeutic approaches for KS.
Advances in human genetics over the past decade have resulted in the identification of hundreds of genes associated with intellectual disability (ID) and autism spectrum disorder (ASD)1. Within this group of genes the number of chromatin regulators is remarkably high2,3,4. These ASD/ID-linked chromatin regulators are engaged in genome-wide covalent DNA modifications, posttranslational modifications of histones, and control of genomic architecture and accessibility5 to control the expression of genes important for neurodevelopment and/or neuroplasticity3. Nevertheless, there is still a large gap between elucidating the genetic architecture of neurodevelopmental disorders (NDDs) and deciphering the cellular or molecular pathobiology6. In particular, we require better understanding of the relevance of genetic changes with respect to downstream functional consequences and whether there is overlap between patients within the clinical spectrum6.
Kleefstra syndrome (KS) (OMIM#610253) is an example of a rare monogenic NDD with ID, ASD, hypotonia, and dysmorphic features7,8,9. KS is caused by heterozygous de novo loss-of-function mutations in the EHMT1 gene (euchromatin histone lysine methyltransferase 1) or by small 9q34 deletions harboring the gene7. In a complex with EHMT2, EHMT1 methylates histone 3 at lysine 9 (H3K9me1 and H3K9me2), promoting heterochromatin formation leading to gene repression10. Constitutive and conditional loss of EHMT1 function in mice and Drosophila lead to learning and memory impairments11,12,13. In addition, Ehmt1+/− mice recapitulate the developmental delay and autistic-like behaviors that are observed in KS patients14,15. At the cellular level, these mice show a significant reduction in dendritic arborization and number of mature spines in CA1 pyramidal neurons11. The dynamic regulation of H3K9me2 by EHMT1/2 is also involved in synaptic plasticity and learning and memory16,17,18. EHMT1 and 2 are required for synaptic scaling, a specific form of homeostatic plasticity, by regulating the expression of brain-derived neurotrophic factor (BDNF)16. Yet it remains unknown how deficits caused by loss of EHMT1 mechanistically affect the development of human neuronal networks.
Human-induced pluripotent stem (iPS) cell technology19 enables us to the study the specific role of individual cell types in developing neural circuits. Patient-derived neurons allow us to examine the early pathophysiology of NDDs using single-cell and neuronal network electrophysiological recordings to recapitulate disease progression20,21.
Here, we generated iPS cell lines from three patients with different EHMT1 loss-of-function mutations to differentiate them into excitatory cortical neurons. Through in-depth characterization at single-cell and neuronal network level, we uncovered a robust and defined phenotype that was consistent across all patient lines and was also observed in neurons with CRISPR-engineered disruption of EHMT1. At the molecular and cellular level, we show that the electrophysiological phenotype is mediated by upregulation of NMDA receptor (NMDAR) subunit 1, which we also find in Ehmt1+/− mice. We conclude by showing that pharmacological inhibition of NMDARs rescues the KS-associated network phenotypes. Therefore, our findings establish a direct link between EHMT1 deficiency in human neurons and NMDAR hyperfunction, providing new insights into the pathophysiology of KS.
Single-cell level characterization of KS neurons
We generated iPS cell lines from two patients with KS and two healthy subjects (respectively KS1, KS2 and C1, C2) (Fig. 1a, Supplementary Fig. 1, Material and Methods). One patient (KS1) had a frameshift mutation in EHMT1 leading to a premature stop codon (p.Tyr1061fs, patient 25 in ref. 22), while the other patient had a missense mutation in the Pre-SET domain (p.Cys1042Tyr, patient 20 in ref. 8), predicted to disrupt the conformation of this domain. As expected Western blot and real-time quantitative polymerase chain reaction (RT-qPCR) analyses revealed a 50% reduction of EHMT1 expression in KS1, while KS2 showed normal EHMT1 expression levels (Fig. 1b, Supplementary Fig. 2A). In addition to these lines, iPS cells were generated from an individual who has a mosaic microdeletion on chromosome 9q34 (233 kb) including EHMT123. We selected an iPS clone harboring the KS-causing mutation (KSMOS) as well as a control clone not carrying the EHMT1 deletion (CMOS) (Fig. 1a, Supplementary Figs. 1 and 2). This isogenic pair shares the same genetic background except for the KS-causing mutation, thereby reducing variability and enabling us to directly link phenotypes to heterozygous loss of EHMT1. Western blot analysis and RT-qPCR analysis showed a 40% reduction of EHMT1 expression in KSMOS compared to CMOS (Fig. 1b, Supplementary Fig. 2A). All selected clones showed positive expression of pluripotency markers (OCT4, TRA-1-81, NANOG, and SSEA4) and single-nucleotide polymorphism (SNP) arrays were performed to confirm genetic integrity (Supplementary Fig. 1, Material and Methods).
We differentiated iPS cells into a homogeneous population of excitatory upper layer cortical neurons (iNeurons) by forced expression of the transcription factor transgene Ngn224. For all experiments, iNeurons were co-cultured with freshly isolated rodent astrocytes25 to facilitate neuronal and network maturation (Fig. 1c). All iPS lines were able to differentiate into MAP2-positive neurons at similar efficiency (Supplementary Fig. 2C, D). EHMT1 expression was reduced in neurons derived from KS1 and KSMOS, but not KS2 (Supplementary Fig. 2B) compared to controls. However, all KS iNeurons showed reduced H3K9me2 immunoreactivity, indicative of EHMT1 haploinsufficiency (Supplementary Fig. 2E). Twenty-one days after the start of differentiation (days in vitro, DIV), both, control and KS iNeurons showed mature neuronal morphology. We measured this by reconstruction and quantitative morphometry of DsRed-labeled iNeurons. We observed no significant differences between control and KS iNeurons in any aspect of neuronal somatodendritic morphology, including the number of primary dendrites, dendritic length and overall complexity (Fig. 1d, Supplementary Fig. 3A–F).
ID and ASD have been associated with synaptic deficits in rodents and humans21,26. We therefore investigated whether EHMT1-deficiency leads to impairments in synapse formation. To this end, we stained control and KS iNeurons for pre- and postsynaptic markers (i.e., synapsin1/2 and PSD95, respectively) at DIV 21. We observed that putative functional synapses were formed on control and KS iNeurons (Supplementary Fig. 3G), without any indications for differences in the number of synaptic puncta between the different iPS cell lines (Fig. 1e, Supplementary Fig. 3G). Furthermore, whole-cell patch-clamp recordings at DIV 21 of iNeurons grown in the presence of tetrodotoxin (TTX) also revealed no differences in the frequency or amplitude of AMPA receptor (AMPAR)-mediated miniature excitatory postsynaptic currents (mEPSCs, Supplementary Fig. 3H). This indicates that KS iNeurons are generally not impaired in the AMPAR component of the excitatory input they receive (Fig. 1f, Supplementary Fig. 3H). At DIV 21 nearly 90% of control and KS patient iNeurons fired multiple APs, indicative of a mature state of their electrophysiological properties (Supplementary Fig. 3I). When recording intrinsic passive and active properties from control and KS patient iNeurons at DIV 21, we found no differences when comparing controls with KS lines. However, we did observe minor differences in AP decay time and Rheobase for the specific lines KS2 and KS1, respectively. These differences were not observed between the isogenic lines (Supplementary Fig. 3J–N, Supplementary Data 1). Collectively, our data indicate that there were no significant differences between control and KS patient iNeurons with regard to neuronal morphology, excitatory synapses and intrinsic properties.
KS neuronal networks show an aberrant pattern of activity
Dysfunction in neuronal network dynamics has been observed in the brain of patients with psychiatric and neurological conditions27. In addition, neuronal network dysfunction has been identified in model systems for several ID/ASD syndromes20,28. Therefore, despite the normal properties of KS iNeurons on single cell level, we hypothesized that impairments during brain development caused by loss of EHMT1 would be reflected at the network level. To test this hypothesis, we examined and compared the spontaneous electrophysiological population activity of neuronal networks derived from controls and KS patients growing on microelectrode arrays (MEAs) (Fig. 2a). MEAs allow us to noninvasively and repeatedly monitor neuronal network activity through extracellular electrodes located at spatially separated points across the cultures.
First, we monitored the in vitro development of control neuronal networks on MEAs. We found that the activity pattern of control iNeuron networks changed progressively over several weeks (Fig. 2b–f), similarly to what was observed in rodent neuronal cultures29. In particular, during the first three weeks of differentiation, control iNeurons mainly showed sporadic APs, i.e., spikes (Fig. 2b–f, DIV 17), indicating they were immature and not yet integrated in a network. By week four electrical activity was organized into rhythmic, synchronous events (network burst), composed of many spikes occurring close in time and across all electrodes (Fig. 2b–f, DIV 24). This indicates that the iNeurons had self-organized into a synaptically connected, spontaneously active network. Both the firing rate, which is a general measure of total activity across the entire network, and network burst activity increased during development but plateaued by DIV 28 (Fig. 2c, d). After this time point neuronal network activity remained stable (Fig. 2c–f). We observed no difference in the overall level or pattern of activity between the C1, C2, and CMOS at DIV 28 (Fig. 2g, i–l). The highly reproducible network characteristics observed across all controls provided us with a consistent, robust standard to which we could directly compare KS-patient derived neuronal networks.
Next, we characterized the neuronal networks derived from KS patients. Similar to controls, network burst activity appeared by the fourth week in vitro (Fig. 2h). The global level of activity of KS networks was similar to controls (i.e., the firing rate, Fig. 2i). However, we found that network bursts occurred at lower frequency and with longer duration (Fig. 2j, k, m, Supplementary Fig. 4A, B). As a consequence of the lower network burst frequency, the inter-burst interval was longer (Fig. 2l, n, Supplementary Fig. 4C, D). We also observed that spike organization differed from controls, which was indicated by the smaller percentage of spikes occurring outside the network bursts (Fig. 2o). A final aspect is that KS networks also exhibited an irregular network-bursting pattern, illustrated by the statistically larger coefficient of variation (CV) of the inter-burst interval (Fig. 2p). Interestingly, the increased burst duration phenotype observed at the network level was also present at single-cell level (Supplementary Fig. S3O). Indeed, whole-cell voltage–clamp recordings of spontaneous excitatory postsynaptic currents (sEPSCs), showed that the activity received by KS-derived neurons was composed by longer burst durations than in control. Taken together, our data show that KS neuronal networks consist of fewer and irregular network bursts, and the bursts themselves were longer in duration than in control networks.
CRISPR/Cas9 deletion of EHMT1 recapitulates KS phenotypes
To further address whether heterozygous loss of EHMT1 is causing the observed KS patient-derived network phenotypes, we expanded our analysis and generated a second set of isogenic human iPS cells. We made use of CRISPR/Cas9 gene editing technology to generate an isogenic control and EHMT1 mutant iPS cell line with a premature stop codon in exon 2 (CCRISPR and KSCRISPR, Fig. 3a, Supplementary Fig. 5F, G). Western blot and RT-qPCR analysis revealed that EHMT1 expression was significantly reduced in KSCRISPR iPS and iNeurons compared to CCRISPR (Fig. 3b, e, Supplementary Fig. 5F, G). Both, CCRISPR and KSCRISPR iPS cells differentiated equally well to iNeurons (Supplementary Fig. 5H). Furthermore, KSCRISPR iNeurons showed reduced H3K9me2 immunoreactivity compared to CCRISPR iNeurons (Supplementary Fig. 5I). We observed no differences in the formation of functional synapses, based on immunocytochemistry and mEPSC recordings between CCRISPR and KSCRISPR, corroborating our results with the other KS cell lines (Fig. 3c, f, Supplementary Fig. 3H). At the network level, CCRISPR showed a control-like network phenotype (Fig. 3d, g–k). KSCRISPR networks exhibited a phenotype similar to the other KS patient networks with less frequent network bursts, longer duration and in an irregular pattern. This establishes a causal role for EHMT1 in the observed neuronal network phenotypes.
Our results demonstrated that EHMT1 deficiency causes a reproducible neuronal network phenotype. We observed only nonsignificant iPS cell line-specific variability in the functional network properties of, both, the control and KS groups, so that the multiple descriptive parameters extracted from the raw MEA recordings clearly delineated control from KS networks. This was confirmed in an unbiased discriminant analysis of network parameters, where control and KS networks clearly clustered away from each other. This separation was not observed when the analysis was performed on single-cell parameters (i.e., morphology and intrinsic properties, Supplementary Fig. 6A–F). Our direct comparison of iNeurons derived from iPS cells with a frameshift, missense or deletion in EHMT1 showed that the phenotype is due to aberrant EHMT1 enzymatic activity rather than the disrupted protein.
KS iNeurons show increased sensitivity to NMDAR antagonists
KS patient-derived neuronal networks showed an aberrant pattern of activity, mainly characterized by network bursts with longer durations than controls. Previous studies on rodent-derived neuronal networks have shown that burst duration is directly influenced by AMPARs and NMDARs. Specifically, previous reports used receptor-type specific antagonists to show that AMPAR-driven bursts have short durations while NMDAR-driven bursts have comparatively longer durations30. We therefore hypothesized that increased NMDAR activity contributed to the lengthened bursts in KS networks. To test this, we pharmacologically blocked either AMPARs or NMDARs and compared the effect on control and KS neuronal network activity at DIV 28. In accordance with previous work30,31, we found that acute treatment with an AMPAR antagonist (NBQX, 50 µM) abolished all network burst activity, whereas inhibiting NMDARs (D-AP5, 60 µM) only slightly decreased the network burst activity (Fig. 4a, c) for control networks. This indicated that network burst activity is mainly mediated by AMPARs. In particular, we found it to be mediated by GluA2-containing AMPARs, since the network bursts were not blocked with Naspm (10 µM), an antagonist that selectively blocks GluA2-lacking AMPARs (Supplementary Fig. 7B, pre-D-AP5). Similar to controls, in KS networks NBQX completely abolished network burst activity (Fig. 4b, d). However, in stark contrast to controls, D-AP5 robustly suppressed the bursting activity in EHMT1 deficient lines (Fig. 4b, d, Supplementary Fig. 7A). Interestingly, the suppression of network burst activity by D-AP5 in KS networks was transient. Network burst activity showed ~50% recovery after 30 min and had returned to baseline (i.e., pre-D-AP5 levels) after 24 h (Fig. 4e, Supplementary Fig. 7C). The early stages of homeostatic plasticity, specifically synaptic upscaling, are initiated by global neuronal inactivity and characterized by insertion of GluA2-lacking AMPARs (i.e., Ca2+-permeable AMPARs, CP-AMPARs) into the synapse to restore activity32. To determine the nature of the recovery, we first blocked KS networks with D-AP5, and added Naspm after 1 h. Naspm completely blocked the reinstated network bursting activity, indicating the recovery in KS networks was due to synaptic insertion of GluA2-lacking AMPARs (Fig. 4e, Supplementary Fig. 7B). Of note, although in control networks burst activity was not suppressed by D-AP5, we did observe that Naspm had a small but significant effect on the burst rate. This indicates that NMDAR blockage in controls also induced less pronounced synaptic insertion of GluA2-lacking AMPARs. After 24 h, the network activity in KS networks was again completely suppressed with NBQX but only partially with Naspm, suggesting that GluA2-lacking AMPARs were actively exchanged with GluA2-containing AMPARs (Supplementary Fig. 7C). Collectively, our results demonstrate that NMDAR inhibition with D-AP5 induces synaptic plasticity in KS networks, allowing reinstating network burst activity through the incorporation of GluA2-lacking AMPARs, which later on are replaced by GluA2-containing AMPARs.
Intrigued by these findings, we sought an alternative way to block network bursting. The classic method for inducing synaptic upscaling, with the sodium channel blocker TTX33, would necessarily prevent observation of early stages of the dynamic recovery on MEAs. To circumvent this issue, we used the antiepileptic drug Retigabine, which is a voltage-gated K+-channel (Kv7) activator that effectively hyperpolarizes the resting membrane potential in neurons34. We reasoned that Retigabine would have the combined effect of acutely hampering neurons in reaching AP threshold while leaving the voltage-gated Na+ channels unaffected. Thus, while simultaneously strengthening the Mg2+ block on NMDARs, we could still observe any (re)occurring network activity on the MEA. Indeed, when we applied Retigabine (10 µM) to the networks, they were temporarily silenced, with no discernible spiking or bursting activity. Within 100 min, there was again a Naspm-sensitive recovery mediated by GluA2-lacking AMPARs occurring in control and KS networks, with an identical pattern (Supplementary Figs. 7D and 8A). This reinforced the notion that the electrophysiological differences we observed earlier between control and KS networks are a direct consequence of NMDAR activity in the KS iNeurons. Furthermore, we found that Retigabine treatment in KS networks decreased the burst length (Supplementary Figs. 7D and 8A), suggesting that Retigabine-induced plasticity allowed KS networks to switch from an NMDAR-dependent to AMPAR-driven bursting, similar to controls.
NMDARs are upregulated in KS iNeurons
The results from our pharmacological experiments suggested that NMDAR expression might be increased in KS iNeurons relative to controls. To test this hypothesis, we measured the transcripts of the most common NMDAR and AMPAR subunits by RT-qPCR for CMOS and KSMOS iNeurons (Supplementary Fig. 7E). We were intrigued to find a fourfold upregulation of GRIN1 mRNA, which encodes NMDAR subunit 1 (NR1), the mandatory subunit present in functional NMDARs. We found no significant changes in any other NMDAR (GRIN2A, GRIN2B, GRIN3A) or AMPAR (GRIA1, GRIA2, GRIA3, and GRIA4) subunit that we analyzed. We further corroborated these results with Western blot analysis, which revealed significantly increased NR1 expression for KSMOS and KSCRISPR iNeurons compared to CMOS and CCRISPR (Fig. 5a, Supplementary Data 1). Our previous functional data indicated that the reduction in methyltransferase activity of EHMT1 was directly responsible for the phenotypes we observed at the network level. Therefore, we used chromatin immunoprecipitation qPCR (ChIP-qPCR) to investigate whether the increased GRIN1 expression correlated with reduced H3K9me2 at the GRIN1 promoter. Our results showed that for KSMOS and KSCRISPR iNeurons H3K9me2 occupancy was reduced at the GRIN1 promotor (Fig. 5b). In accordance with our previous study in Ehmt1+/− mice16, we also found that the occupancy at the BDNF promoter was reduced in KSMOS and KSCRISPR iNeurons (Fig. 5b). Next, using immunocytochemistry, we found that NR1 was significantly increased in KSMOS and KSCRISPR compared to CMOS and CCRISPR iNeurons (Fig. 5c). Finally, we investigated whether the increased NR1 level also resulted in increased synaptic NMDAR activity. To this end we infected control or KS iNeurons at 7 DIV with an adeno-associated virus (AAV2) expressing mCherry-tagged channelrhodopsin (±80% infection efficiency). We recorded from uninfected cells in voltage clamp at a holding potential of −70 mV (AMPAR) or +40 mV (NMDAR) and measured (blue)light-evoked synaptic responses by exciting nearby channelrhodopsin-expressing cells (mCherry positive) (Fig. 5d). The NMDAR/AMPAR ratio showed to be significantly increased in KSMOS and KSCRISPR iNeurons (Fig. 5e). This increase in NMDAR/AMPAR ratio is likely due to an increased NMDAR activity since frequency and amplitude of AMPAR-mediated mEPSCs remained unchanged (Supplementary Fig. 3H).
Taken together, our data show that the loss of EHMT1 activity in KS lines results in a reduction of the repressive H3K9me2 mark, causing an upregulation of NR1 and increased synaptic NMDAR activity.
Altered neuronal network activity in Ehmt1+/− mice
Having established that EHMT1-deficiency alters neuronal network activity due to NR1 upregulation in KS iNeurons, we next set out to measure neuronal network activity in Ehmt1+/− mice, a validated mouse model that recapitulates the core features of KS14,15. Similar to what we found in iNeurons, primary cultures of cortical neuronal networks derived from Ehmt1+/− mice showed network bursts with lower frequency and longer duration compared to cultures from littermate controls. The MFR was unaltered (Fig. 6a). Using whole-cell voltage clamp recordings in acute brain slices, we measured the ratio between AMPAR- and NMDAR-mediated currents from cortical Layer 4 to Layers 2/3 synapses. We found that the NMDAR/AMPAR ratio was significantly increased in cortical networks of Ehmt1+/− mice compared to WT littermates (Fig. 6b). We found no changes in the kinetics of NMDAR-mediated currents, suggesting that there is no difference in the expression of NMDAR subunits 2A or 2B between WT and Ehmt1+/− mice (Supplementary Fig. 6B)35. Finally, we found no changes in the frequency or amplitude of AMPAR-mediated mEPSCs suggesting that the increased NMDAR/AMPAR ratio in Ehmt1+/− mice is due to increased NMDAR activity (Fig. 6c).
NMDAR inhibition rescues KS neuronal network phenotypes
Our previous experiments showed that by inhibiting NMDARs in KS networks for 24 h, we could shift the balance so that neuronal networks were progressively driven by GluA2-containing AMPARs, similar to controls (Supplementary Fig. 7C). Based on these results, we reasoned that the phenotypes in KS networks could be rescued by chronically inhibiting NMDARs in mature neuronal networks. We chose to potently block the channel pore of NMDARs, and thereby primarily inhibiting postsynaptic calcium flux, with the selective, noncompetitive open-channel blocker MK-80136. The immediate effects of MK-801 (1 µM) on KS network activity were similar to D-AP5, where network bursting was transiently suppressed and then recovered by insertion of GluA2-lacking AMPARs, which were again completely inhibited by Naspm (Fig. 7a, Supplementary Fig. 8B). Next, we treated CMOS and KSMOS networks beginning at DIV 28 for 7 days with MK-801. We found that chronically blocking NMDARs in KSMOS networks reversed the major network parameters that we measured in KSMOS iNeurons, bringing them closer to controls (Fig. 7b–g). More specifically, we observed an increased burst frequency (Fig. 7d), with a parallel reduction in the inter-burst interval (Fig. 7f). Furthermore, the network burst duration (NBD) was reduced (Fig. 7e) and the network bursting became more regular (Fig. 7g) showing values similar to control, indicative of a shift towards AMPAR-driven networks. A discriminant analysis based on the aforementioned parameters showed that the KSMOS networks treated with MK-801 for 7 days became segregated from untreated KSMOS networks and closer to CMOS networks (Fig. 7h). Similar effects of MK-801 treatment were also observed in KSCRISPR (Supplementary Fig. 8C–G). These results indicate that the aberrant activity pattern of KS patient-derived neuronal networks can be rescued by specifically inhibiting NMDAR activity.
In this study, we developed a human model of KS that enabled us to identify specific functional aberrations, from gene expression to neuronal network behavior, due to EHMT1 haploinsufficiency. We found that excitatory networks derived from KS patients showed a distinct and robust neuronal network phenotype with striking similarity across different types of mutations in EHMT1. The phenotype was characterized by network bursts with a longer duration, lower frequency and more irregular pattern compared to controls. At the cellular level, we demonstrated that the network phenotype was mediated by NR1 upregulation.
Interestingly, the neuronal phenotype was consistent across species and model systems. Indeed, we found that network bursts also occurred with a longer duration and irregular pattern in dissociated neuronal networks from either embryonic rats (i.e., where Ehmt1 was downregulated through RNA interference29,37) or Ehmt1+/− mice. This indicates that some network parameters are consistently and similarly altered in divergent KS models. The appearance of a consistent phenotype both in a system where excitatory and inhibitory transmission were present and in a system where inhibition was absent (human iNeurons) indicates a major contribution from aberrant excitatory neurotransmission to KS pathobiology.
One major characteristic of KS networks was the longer duration of the network bursts. A change in burst length can be indicative of synaptic changes in GABARs, NMDARs, and/or AMPARs31. Given that inhibition was absent in our human model, we focused our analysis on NMDARs and AMPARs. We present several lines of evidence that suggest the long bursts exhibited by KS networks are mediated by upregulation of NR1. First, by acutely blocking AMPARs and NMDARs with chemical inhibitors, we show that network burst activity in KS networks is strongly dependent on NMDAR-mediated transmission, in contrast to control networks, where network bursts are mainly dependent on AMPAR-mediated transmission. Second, we were able to reverse KS network phenotypes, including the long NBD, by blocking NMDAR activity. Third, we found that NR1 is upregulated in KS iNeurons at both, mRNA and protein level. Fourth, NR1 upregulation is paralleled by H3K9me2 hypomethylation at the GRIN1 promoter. Fifth, NMDAR/AMPAR ratio was increased in KS iNeurons. Finally, we found increased NMDAR-mediated currents in the cortex of Ehmt1+/− mice. This cross-species comparison further validates that observed effects are due to decreased EHMT1 enzymatic function and show that development of network activity in human and mouse cortical networks may follow evolutionarily conserved and stable epigenetic programming.
Genetic evidence has directly implicated NMDARs in NDDs. For example, multiple heterozygous mutations in NMDAR subunit genes have been identified to be causal for ID, ASD, or epilepsy38. NMDAR dysfunction is mainly attributed to hypofunction, but there are observations associating NMDAR hyperfunction to ID/ASD:39 upregulated NR1 protein levels were found in the cerebellum of ASD patients40 and the NR2A and NR2B subunits of the NMDAR were found to be upregulated in the valproic acid animal model of autism41. In the Rett syndrome mouse model42, loss of MECP2 function resulted in developmental dysregulation of NMDAR expression35,43. Of note, work in mouse models of NDDs show that the changes in NMDARs expression are temporally and spatially restricted. This illustrates the importance of evaluating at what developmental age and in which brain regions changes in NMDAR expression occur, which is especially relevant for the design of rescue strategies44,45,46,47,48. Our data shows that NR1 is upregulated in KS iNeurons of cortical identity and support the idea that dysfunctional glutamatergic neurotransmission plays a role in NDDs.
By blocking NMDARs in KS networks, we were able to induce the early phase of synaptic upscaling enabling the incorporation of GluA2-lacking receptors, followed by the insertion of GluA2-containing receptors. This plasticity mediated by AMPARs was more easily initiated in KS than in control networks and allowed KS networks to switch, at least temporarily, from a mainly NMDAR-driven network to an AMPAR-driven network. Interestingly, we also observed that Naspm had an effect on control cells that were treated with NMDAR antagonists, suggesting that GluA2-lacking receptor were inserted and/or possibly exchanged for GluA2-containing receptors after NMDAR antagonist treatment. Together, this suggests that loss of EHMT1 could facilitate NMDAR-mediated plasticity after a comparatively milder stimulus, in this case NMDAR blockade with D-AP5. This is in agreement with a recent study showing that inhibition of EHMT1/EHMT2 activity reinforces early LTP in an NMDAR-dependent manner18. The authors showed that pharmacologically blocking EHMT1/2 before a mild LTP stimulus increased the LTP response, highlighting a role for EHMT1 and associated H3K9me2 in metaplasticity18. A link between NMDARs and EHMT1 has also been shown in vivo where NMDAR activity regulates the recruitment of EHMT1/2 and subsequent H3K9me2 levels at target gene promoters in the lateral amygdala, in the context of fear learning49. This, together with our data, suggests that there is a reciprocal interaction, between NMDAR activity and EHMT1 function, and a positive-feedback mechanism where EHMT1 methylates the NR1 promoter upon activation by NMDARs. If and under which circumstances such a feedback mechanism is active during development and/or in adulthood deserves further investigation.
At the molecular level we and others have shown that EHMT1 haploinsufficiency can cause modest transcriptional changes12,16,37,50. We found that the upregulation of NR1 correlates with reduced H3K9me2 occupancy at the GRIN1 promoter. This suggests that during normal development EHMT1 is directly involved in the regulation of NR1 expression. However, we cannot exclude that EHMT1 also regulates NR1 expression via other, less direct mechanisms. For example, EHMT1 is a member of a large complex that includes the neuron-restrictive silencer factor (NRSF/REST) repressive unit, which is important for repressing neuronal genes in progenitors, including NR151,52,53. In addition, growth factors such as BDNF, which is increased upon EHMT1 deletion16, have been shown to increase NR1 mRNA levels in cultured embryonic cortical neurons54. Finally, we know from previous studies that EHMT1 regulates genome-wide H3K9me2 deposition, altering the expression of multiple genes12,16,37,50.
An important aspect of our study is the identification of a robust and consistent network phenotype linked to KS on MEAs. We show that KS networks differed from controls based on a set of parameters describing the neuronal network activity and using discriminant analysis. Our analysis showed that individual unrelated controls clustered with little variation, which was exemplified by the fact our predictive group membership analysis was able to accurately assign control networks to the control group (Fig. S6). In contrast, we found that KS patient lines significantly differed from controls, including their respective isogenic controls (CMOS vs. KMOS and CCRISPR vs. KSCRISPR). It should be noticed here that the 233 kB deletion in KSMOS incorporates another gene (CACNA1B) that might affect neuronal function, which might explain minor changes compared to the other KS lines. When we performed predictive group membership analysis, we found that individual KS networks were mostly assigned to the corresponding patient line (Fig. S6), indicating that this level of analysis on neuronal networks can potentially detect patient-specific phenotypic variance that arises early in development. It is foreseeable that, in future studies, an in-depth interrogation of the network activity for networks consisting of different human derived cell types or networks from brain organoids would allow measuring more complex neuronal signals on MEAs. This would be especially relevant for the stratification of genetically complex disorders (e.g., idiopathic forms of ASD) as these in vitro network phenotypes could then be used as endophenotype in pharmacological studies.
We show that it is possible to rescue the neuronal phenotype by blocking NMDARs in mature networks, a finding that has important clinical relevance. For example, NMDAR antagonists like ketamine and memantine have been used successfully in mouse models to treat RTT46,47 and other NDDs45,48 and led in some cases to improvements in small open-label trials for autism55,56,57. These studies, as well as ours, provide preclinical proof of concept that NMDAR antagonists could ameliorate neurological dysfunction and reverse at least some circuit-level defects. Furthermore, our observation that neuronal phenotypes can be rescued in mature networks agrees with previous data showing that reinstating EHMT1 function in adult flies is sufficient to rescue memory deficits13. This adds to a growing list of genetically defined ID syndromes that might be amenable to postnatal therapeutic intervention58.
Summarized, our study shows that combining iPS cell-derived human neuronal models with neuronal network dynamics is a promising tool to identify novel targets for possible treatment strategies for NDDs, such as ID and ASD.
Patient information and iPS cell generation
In this study we used in total four control and four iPS cells with reduced EHMT1 function. In contrast to a previous study59 we included patients in this study that present the full spectrum of KS associated symptoms, including ID and ASD. KS1 and KS2 originate from two individuals: a female KS patient with a frameshift and a missense mutation in the EHMT1 gene, respectively (patient 25 in ref. 22 and patient 20 in ref. 8). An isogenic pair of control (CMOS) and KS line (KSMOS) originated from an individual diagnosed with a mosaic heterozygous 233 kbp deletion of chromosome 9 which includes the EHMT1 gene (deletion starts after exon 4) and CACNA1B gene23. Clones were selected after performing RT-qPCR on genomic DNA with primers spanning Exon 3: F-GAAGCAAAACCACGTCACTG; R-GTAGTCCTCAAGGGCTGTGC. Exon 4: F-CCCAGAGAAGTTCGAGAAGC, R- GGGTAAAAGCTGCTGTCGTC. Exon 5 F-CAGCTGCAGTATCTCGGAAG, R- AACATCTCAATCACCGTCCTC. Exon 6 F- GACTCGGATGAGGACGACTC, R- GGAAGTCCTGCTGTCCTCTG. All iPS cells used in this study were obtained from reprogrammed fibroblasts. For most of the lines reprogramming to iPS cells was induced by retroviral vectors expressing four transcription factors: Oct4, Sox2, Klf4, and cMyc. For control line (C2), which was obtained from Mandegar et al.60, episomal reprogramming was performed, whereas the CCRISPR line was generated by non-integrating Sendai virus. Generated clones (at least two per patient line) were selected and tested for pluripotency and genomic integrity based on SNP arrays. IPS cells were always cultured on Matrigel (Corning, #356237) in E8 flex (Thermo Fisher Scientific) supplemented with primocin (0.1 µg/ml, Invivogen) and low puromycin (0.5 µg/ml) and G418 concentrations (50 µg/ml) at 37 °C/5% CO2. Medium was refreshed every 2–3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies). Collecting patient material and establishing hiPSCs have all been performed according to locally (Radboudumc) IRB protocols.
CRISPR/Cas9 editing of EHMT1
We made use of the CRISPR/Cas9 technology in order to create a heterozygous EHMT1 mutation in Exon 2 in a iPS cell line derived from a healthy 51-year-old male to mimic KS in isogenic cell lines, generated by KULSTEM (Leuven, Belgium). In brief, 1 × 106 iPSCs in single-cell suspension were nucleofected with Cas9 ribonucleoprotein complexes (10 µg/reaction S.p. Cas9 Nuclease 3NLS (IDT 1074181), CRIPSPR crRNAs (IDT) and CRISPR tracrRNA (IDT 1072532) 0.4 nmoles each/reaction) and a donor vector (5 µg/ reaction). The two crRNAs were designed to specifically target EHMT1 (TCTAACAGGCAGTTCCGGCGAGG and TAACAGGCAGTTCCGGCGAGGGG). The donor vector was a piggyback construct containing a hygromycin selection cassette as well as sequences that enable homology-directed repair ensuring the insertion of premature stop codons in Exon 2 of the EHMT1 gene. Cells were nucleofected using the Human Stem Cell Nucleofector® Kit 2 (Lonza, VPH-5022) in combination with the AMAXA-2b nucleofector, program F16. After nucleofection cells were resuspended in E8 flex (Thermo Fisher Scientific) supplemented with Revitacell (Thermo Fisher Scientific). When the iPS cells reached a confluency of about 40% selection was started using 5 µg/ml hygromycin. The antibiotics concentration was increased to up to 200 µg/ml over 2 weeks. Hygromycin resistant colonies were picked and PCR validation was used to ensure heterozygous editing of Exon2. To prove reduced EHMT1 expression in the CRISPR/Cas9 edited clone RT-qPCR and Western Blot were performed to measure EHMT1 protein levels in CCrispr and KSCrispr. DNA fingerprinting profile was performed on both lines by qPCR detection of a reference set of SNP panel using the TaqMan assays from Life Technologies. The genome-edited iPS cell line shows identical SNP profile with the corresponding iPS cell line used for gene targeting. Off-target of genome editing was verified by sequencing the top four off-target sites of each gRNA have been sequenced and no INDELs were detected (Supplementary Fig. 5). The generated iPS cells were validated for pluripotency markers and quantitative analysis of tri-lineage differentiation potential was performed. All generated iPS cell lines have the capacity to differentiate toward all three germ layers (endoderm, mesoderm, ectoderm). To this end embryoid bodies were generated in 24-well Corning low attachment plates. For spontaneous differentiation, the culture was kept for 7 days in E6 medium (Thermo Fisher Scientific). The medium was changed every 2 days. Cells were harvested after 7 days for RNA extraction with the GenElute Mammalian Total RNA kit (Sigma). cDNA synthesis was performed with Superscript III and used for qPCR according to manufacturer’s protocol with TaqMan human iPS cell Scorecard assay (Life Technologies). Data analysis was performed with Scorecard software (online tool Life technologies), comparing with a reference set of pluripotent stem cell lines.
For Western Blot cell lysates were made from confluent wells in six-well plates of either iPS cells or iNeurons. Medium was always refreshed 4 h beforehand. Protein samples were loaded, separated by sodium dodecyl supfate (SDS) polyacrylamide gel electrophoresis, and transferred to nitrocellulose membrane (BIO-RAD). The membrane was then probed with an EHMT1 antibody (1:1000; Abcam ab41969) or NMDAR1 (1:100; Biolegend 818601). To control for loading differences, we probed with anti-gamma tubulin (1:1000; Sigma T5326) or GAPDH (1:1000; Cell Signaling #2118). For visualization horseradish peroxidase-conjugated secondary antibodies were used (1:20000 for both; goat anti-mouse: Jackson ImmunoResearch Laboratries, 115-035-062. Goat-anti rabbit: Invitrogen, A21245).
iPS cells were directly derived into, excitatory cortical Layer 2/3 neurons by overexpressing the neuronal determinant Neurogenin 2 (NGN2) upon doxycycline treatment based on Zhang et al.24 and as we described previously25. To support neuronal maturation, freshly prepared rat astrocytes25 were added to the culture in a 1:1 ratio two days after plating. At DIV 3 the medium was changed to Neurobasal medium (Thermo Fisher Scientific) supplemented with B-27 (Thermo Fisher Scientific), glutaMAX (Thermo Fisher Scientific), primocin (0.1 µg/ml), NT3 (10 ng/ml), BDNF (10 ng/ml), and doxycycline (4 µg/ml). Cytosine b-d-arabinofuranoside (Ara-C) (2 µM; Sigma) was added once to remove any proliferating cell from the culture. From DIV 6 onwards half of the medium was refreshed three times a week. The medium was additionally supplemented with 2.5% FBS (Sigma) to support astrocyte viability from DIV 10 onwards. Neuronal cultures were kept through the whole differentiation process at 37 °C/5%CO2.
Cortical cultures from mice
Primary cortical neurons were prepared from Ehmt1+/− and Ehmt1+/+ mice from individual E16.5 embryos as previously described16. Since the genotype was unknown at the time of harvest, each embryo was collected and the brains were processed separately. Each whole brain was kept on ice in 1 mL L-15 medium, organized separately in a 24-well plate, and tail clips were collected for genotyping.
Neuronal morphology reconstruction
To examine morphology of neurons cells on coverslips were transfected with plasmids expressing Discosoma species red (dsRED) fluorescent protein one week after plating. DNAin (MTI-GlobalStem) was used according to manual instructions. Medium was refreshed completely the day after DNAin application. After the treatment cells were cultured as previously described.
After 3 weeks of differentiation cells were fixed in 4% paraformaldehyde/4% sucrose (v/v) in phosphate-buffered saline (PBS) and mounted with DAKO. Transfected neurons were imaged using a Zeiss Axio Imager Z1 and digitally reconstructed using Neurolucida 360 software (MBF–Bioscience, Williston, ND, USA). For large cells multiple 2-dimensional images of these neurons were taken and subsequently stitched together using the stitching plugin of FIJI 2017 software. The three-dimensional reconstructions and quantitative morphometrical analyses focused on the somatodenritic organization of the neurons. We defined origins for individual primary dendrites by identifying emerging neurites with diameters that were less than 50% of the diameter of the soma. Axons, which were excluded from reconstructions and further analysis, we visually identified by their long, thin properties, far reaching projections and numerous directional changes Neurons that had at least two primary dendrites and reached at least the second branching order were considered for analysis. For morphometrical analysis we determined soma size, number of primary dendrites, length and branching points per primary dendrite and total dendritic length. To measure the total span of the dendritic field (receptive area) of a neuron we performed convex hull 3D analysis. Note, that due to the two-dimensional nature of the imaging data, we collapsed the convex hull 3D data to two-dimensions, resulting in a measurement of the receptive area and not the volume of the span of the dendritic field. Furthermore, Sholl analysis was performed to investigate dendritic complexity in dependence form distance to soma. For each distance interval (10 µm each) the number of intersections (the number of dendrites that cross each concentric circle), number of nodes and total dendritic length was measured. Discriminant function analysis with canonical discriminant functions and reclassification of group membership based on parameters describing neuronal morphology were performed in SPSS.
Cells were fixed with 4% paraformaldehyde/4% sucrose (v/v) for 15 min and permeabilized with 0.2% triton in PBS for 10 min at RT. Nonspecific binding sites were blocked by incubation in blocking buffer (PBS, 5% normal goat serum, 1% bovine serum albumin, 0.2% Triton) for 1 h at RT. Primary antibodies were diluted in blocking buffer incubated overnight at 4 °C. Secondary antibodies, conjugated to Alexa-fluorochromes, were also diluted in blocking buffer and applied for 1 h at RT. Hoechst 33342 (Molecular Probes) was used to stain the nucleus before cells were mounted using DAKO fluorescent mounting medium (DAKO). Neurons were fixed at DIV 21 for synaptic stainings. Used primary antibodies were: mouse anti-MAP2 (1:1000; Sigma M4403); guinea pig anti-MAP2 (1:1000; Synaptic Systems 188004); guinea pig anti-synapsin 1/2 (1:1000; Synaptic Systems 106004); mouse anti-PSD95 (1:50; Thermo Fisher Scientific MA1-045); rabbit anti-GFAP (1:500; Abcam ab7260), mouse anti-pan axon (1:1000; Covance SMI-312R), mouse anti-NMDAR1 (1:1000; ThermoFisher Scientific 54.1). Secondary antibodies that were used are: goat anti-guinea pig Alexa Fluor 647 (1:2000, Invitrogen A-21450); goat anti-rabbit Alexa Fluor 488 (1:2000, Invitrogen A-11034); goat anti-rabbit Alexa Fluor 568 (1:2000, Invitrogen A-11036); goat anti-mouse Alexa Fluor 488 (1:2000, Invitrogen A-11029); goat anti-mouse Alexa Fluor 568 (1:2000, Invitrogen A-11031). We imaged at a 63× magnification using the Zeiss Axio Imager Z1 equipped with apotome. All conditions within a batch were acquired with the same settings in order to compare signal intensities between different experimental conditions. Signals were quantified using FIJI software. The number of synaptic puncta was determined per individual cell via manual counting and divided by the dendritic length of the neuron.
To investigate H3K9me2 iNeurons were fixed at DIV3. Used primary antibodies are: rabbit anti-H3K9me2 antibody (1:500, Millipore 07-441), and guinea pig anti-MAP2 antibody (1:1000, Synaptic Systems 188004). Secondary antibodies used are: goat anti-rabbit Alexa Fluor 488 (1:2000, Invitrogen A-11034) and goat anti-guinea pig Alexa Fluor 647 (1:2000, Invitrogen A-21450). Epifluorescent images were taken with the same exposure time using Zeiss Axio Imager Z1 with apotome. H3K9me2 was assessed based on color intensity measurements by ImageJ analysis tools.
Quantification of mRNA by RT-qPCR
RNA samples were isolated from both, iPS cells or iNeurons (DIV28) using Nucleo Spin RNA isolation kit (Machery Nagel, 740955.250) according to the manufacturer’s instructions. RNA samples (200 ng) were converted into cDNA by iScript cDNA synthesis kit (BIO-RAD, 1708891). cDNA products were cleaned up using the Nucleospin Gel and PCR clean-up kit (Machery Nagel, 740609.250). Human-specific primers were designed with Primer3plus and IDT PrimerQuest tools, respectively. Primer sequences are given in supplementary Table 1. qPCRs were performed in the 7500 Fast Real Time PCR System apparatus (Applied Biosystems) with GoTaq qPCR master mix 2× with SYBR Green (Promega, A600A) according to the manufacturer’s protocol. The PCR program was designed as following: After an initial denaturation step at 95 °C for 10 min, PCR amplifications proceeded for 40 cycles of 95 °C for 15 s and 60 °C for 30 s and followed by a melting curve. All samples were analyzed in duplicate in the same run, placed in adjacent wells. Reverse transcriptase-negative controls and no template-controls were included in all runs. The arithmetic mean of the Ct values of the technical replicas was used for calculations. Relative mRNA expression levels were calculated using the 2−ΔΔCt method61 with standardization to housekeeping genes.
Chromatin immunoprecipitation (ChIP)-qPCR
ChIP was performed according to the Blueprint-IHEC (international human epigenome consortium) protocol with slight modifications. Briefly, 5,00,000 cells were used for one immunoprecipitation reaction. Cells were first crosslinked with disuccinimidyl glutarate at a final concentration of 2 mM for 30 min at RT followed by second crosslinking treatment with 1% formaldehyde for 8 min at RT. Crosslinking was stopped by adding glycine to a final concentration of 0.125 M for 10 min. Samples were washed and homogenized in cold lysis buffer (10 mM Tris (pH 8.0), 0.2%NP40, 0.2% TritonX-100, 1 mM EDTA, 0.5 mM EGTA, 5× protease inhibitors EDTA-free, and 10 mM NaCl) for 15 min on ice. Nuclei were lysed with nuclei lysis buffer (1 mM EDTA, 0.5 mM EGTA, 50 mM HEPES) for 20 min rotating at 4 °C. After centrifugation the collected extracts were resuspended in sonication buffer (20 mM HEPES pH = 7.6, 1% SDS, 2.5× protease inhibitors EDTA-free) and sonicated with Bioruptor Power-up (Diagenode) to shear DNA into 150–700 bp fragments. Cleaned-up chromatin was diluted with ChIP dilution buffer (0.15% SDS, 1% TritonX-100, 1.2 mM EDTA, 16.7 mM Tris pH = 8, 167 mM NaCl). Immunoprecipitation was performed with anti-H3K9me2 (5 µg/reaction, Abcam ab1220) and anti-EHMT1 (15 µg/reaction, Abcam ab41969) antibodies overnight rotating at 4 °C. As a negative control, anti-human IgG (10 µg/reaction, Abcam ab2410) was used in place of specific antibodies. Chromatin was enriched by 10 μL of each Protein A (Invitrogen, 10001D) and Protein G Dynabeads (Invitrogen, 10003D) for 2 h rotating at 4 °C. Beads were washed once with wash buffer 1 (2 mM EDTA, 20 mM Tris pH = 8, 1% TritonX-100, 0.1% SDS, 150 mM NaCl), twice with wash buffer 2 (2 mM EDTA, 20 mM Tris pH = 8, 1% TritonX-100, 0.1% SDS, 500 mM NaCl), twice with wash buffer 3 (1 mM EDTA, 10 mM Tris pH = 8) and eluted in elution buffer (1% SDS; 100 mM NaHCO3) by rotating for 20 min at RT. Eluate was de-crosslinked in elution buffer with 40 µg proteinase-K and 200 mM NaCl overnight at 65 °C. DNA material was cleaned with QIAquick MinElute PCR Purification Kit (Qiagen, 28006) according to manufacturer’s instructions. Five microlitreof eluted DNA sample was used for qPCR analysis. qPCRs were performed as described earlier. For primer sequences see Supplementary Table 2. All samples were analyzed in duplicate in the same run and placed in adjacent wells. ChIP-qPCR results are expressed as % input, which was calculated by the following formula: 100 × 2−ΔCt, where ΔCt = Ct[ChIP] − (Ct[input] − Log2(input dilution factor)).
MEA recordings and data analysis
All recordings were performed using the 24-well MEA system (Multichannel Systems, MCS GmbH, Reutlingen, Germany). MEA devices are composed by 24 independent wells with embedded microelectrodes (i.e., 12 electrodes/well, 80 µm in diameter and spaced 300 µm apart). Spontaneous electrophysiological activity of iPS cell-derived neuronal network grown on MEAs was recorded for 20 min. During the recording, the temperature was maintained constant at 37 °C, and the evaporation and pH changes of the medium was prevented by inflating a constant, slow flow of humidified gas (5% CO2 and 95% O2) onto the MEA plate (with lid on). The signal was sampled at 10 kHz, filtered with a high-pass filter (i.e., Butterworth, 100 Hz cutoff frequency) and the noise threshold was set at ±4.5 standard deviations.
Data analysis was performed off-line by using Multiwell Analyzer (i.e., software from the 24-well MEA system that allows the extraction of the spike trains) and a custom software package named SPYCODE developed in MATLAB (The Mathworks, Natick, MA, USA) that allows the extraction of parameters describing the network activity62.
The mean firing rate (MFR) of the network was obtained by computing the firing rate of each channel averaged among all the active electrodes of the MEA. Burst detection: bursts were detected using a Burst Detection algorithm. The algorithm is based on the computation of the logarithmic inter-spike interval histogram in which inter-burst activity (i.e., between bursts and/or outside bursts) and intra-burst activity (i.e., within burst) for each recording channel can be easily identified, and then a threshold for detecting spikes belonging to the same burst is automatically defined. From the burst detection, the number of bursting channels (above threshold 0.4 burst/s and at least 5 spikes in burst with a minimal inter-spike-interval of 100 ms) was determined. Network burst detection: synchronous events were detected by looking for sequences of closely spaced single-channels bursts. A network burst was defined as burst that occurs in >80% of the network active channels. The distributions of NBDʼs and network inter-burst interval (NIBI, interval between two consecutive network bursts) were computed using bins of 100 ms and 1 s respectively. Network burst irregularity: irregularity was estimated by computing the CV of the NIBI, which is the standard deviation divided by the mean of the NIBI. Discriminant function analysis with canonical discriminant functions and reclassification of group membership based on parameters describing neuronal network activity were performed in SPSS.
All reagents were prepared fresh into concentrated stocks as indicated below, and stored frozen at −20 °C. The following compounds were used in pharmacological experiments: 1-Naphthyl acetyl spermine trihydrochloride (“Naspm”, 100 mM in PBS, Tocris Cat No 2766); (+)-MK 801 maleate (“MK-801”, 100 mM in DMSO, Tocris Cat No 0924); 2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide (“NBQX”, 100 mM in DMSO, Tocris Cat No 0373); D-2-amino-5-phosphonovalerate (“D-AP5”, 50 mM in PBS, Tocris Cat No 0106); Retigabine (100 mM in DMSO, Tocris Cat No. 6233). For all experiments on MEAs, and immediately before adding a compound to the cells, an aliquot of the concentrated stock was first diluted 1:10 in room temperature DPBS and vortexed briefly. Then, the appropriate amount of working dilution was added directly to wells on the MEA and mixing was primarily through diffusion into the (500 µl) cell culture medium. Of note, where relevant the DMSO concentration in the medium was always ≤0.05% v/v.
Control and KS patient neuronal networks were treated acutely with D-AP5 (60 µM), NBQX (50 µM), MK-801 (1 µM), Naspm (10 µM), or Retigabine (10 µM) at DIV 28 after a 20-min recording of spontaneous activity. Then, the recording was paused, the compounds were added to the MEA, and the recording was restarted after 5 min. We recorded neuronal network activity for 60 min with D-AP5 or NBQX, 90 min with MK-801, 100 min with Retigabine, and for 20 min after the addition of Naspm. In experiments where we examined the effect of chronic NMDAR blockade, control and KS patient neuronal networks were treated with 1 µM MK-801 starting at DIV 28 and lasting 7 days total. MK-801 was replenished every two days, where it was freshly diluted to 1 µM in complete Neurobasal medium for the routine half-medium change. All experiments were performed at 37 °C.
For single-cell recordings we used neurons derived from C1, C2, CMOS, CCRISPR, and KS1, KS2, KSMOS, and KSCRIPSR after 3 weeks of differentiation. Experiments were performed in a recording chamber on the stage of an Olympus BX51WI upright microscope (Olympus Life Science, PA, USA) equipped with infrared differential interference contrast optics, an Olympus LUMPlanFL N 40× water-immersion objective (Olympus Life Science, PA, USA) and kappa MXC 200 camera system (Kappa optronics GmbH, Gleichen, Germany) for visualization. Recordings were made using a SEC-05× amplifier (NPI Electronic GmbH, Tamm, Germany), low-pass filtered at 3 kHz and digitized at 10 kHz using a 1401 acquisition board and recorded with Signal (CED, Cambridge, UK). Recordings were not corrected for liquid junction potential (±10 mV).
We performed the recordings of neurons cultured on cover slips under continuous perfusion with oxygenated (95% O2/5% CO2) artificial cerebrospinal fluid (ACSF) at 30 °C containing (in mM) 124 NaCl, 1.25 NaH2PO4, 3 KCl, 26 NaHCO3, 11 Glucose, 2 CaCl2, 1 MgCl2 (adjusted to pH 7.4). Patch pipettes (6–8 MΩ) were pulled from borosilicate glass with filament and fire-polished ends (Science Products GmbH, Hofheim, Germany) using the PMP-102 micropipette puller (MicroData Instrument, NJ, USA). For current clamp recordings of the intrinsic electrophysiological properties, we filled pipettes with a potassium-based solution containing 130 mM K-Gluconate, 5 mM KCl, 10 mM HEPES, 2.5 mM MgCl2, 4 mM Na2-ATP, 0.4 mM Na3-ATP, 10 mM Na-phosphocreatine, 0.6 mM EGTA (adjusted to pH 7.25 and osmolarity 290 mosmol). We determined the resting membrane potential (Vrmp) after achieving whole-cell configuration and only considered neurons with Vrmp of −55 mV and lower for further testing and analyses. Recordings were not corrected for liquid junction potentials of ca. −10 mV. All further electrophysiological characterizations we performed at a holding potential of −60 mV. We determined the passive membrane properties via a 0.5 s hyperpolarizing current of −25 pA. Active intrinsic properties, i.e., action potential (AP) firing characteristics we derived from the first AP elicited by a 0.5 s depolarizing current injection just sufficient to reach AP threshold. For voltage clamp recordings we filled pipettes with a cesium-based solution containing 115 mM cesium methanesulfonate, 20 mM CsCl, 10 mM HEPES, 2.5 mM MgCl2, 4 mM Na2ATP, 0.4 mM Na3GTP, 10 mM sodium phosphocreatine, and 0.6 mM EGTA adjusted to a pH of 7.4. Spontaneous AP-sEPSC were recorded in drug-free ACSF. Bursts were detected when at least 5 events with a minimal inter-event-interval of 100 ms were exhibited by a cell. Burst detection, burst frequency and duration were computed with software developed in MATLAB (The Mathworks, Natick, MA, USA). mEPSC were measured in voltage clamp at a holding potential of −60 mV in the presence of 1 μM TTX (Tocris, Bristol, UK) and 100 μM picrotoxin (Tocris, Bristol, UK). Evoked responses (AMPAR and NMDAR) were measured by infecting iNeurons at DIV 7 with 0.5 µl AAV-mCherry-Chr2 (UNC Vector Core, USA). Uninfected cell were recorded in voltage clamp at a holding potential of −70 mV (AMPAR) or +40 mV (NMDAR). Light-evoked synaptic responses were induced by exciting nearby infected cells (mCherry positive) with blue light (COOLLED pE-200; 10 ms, 470 nm). Stimulus strength, location of stimulus were adjusted to 30–50 pA postsynaptic AMPAR-mediated responses at −70 mV holding voltage, and 30 sweeps were averaged for the final trace. AMPA amplitudes were quantified as the peak amplitude at −70 mV holding voltage, and the NMDA amplitudes were quantified as the average of 5 ms of the trace 65 ms after the stimulus to prevent contamination of AMPAR-mediated response at +40 mV. NMDA/AMPA ratios were calculated by averaging responses in Clampfit (vs. 10.7, Molecular Devices, CA, USA). Intrinsic electrophysiological properties were analyzed using Signal and MatLab (MathWorks, MA, USA), while mEPSCs were analyzed using MiniAnalysis 6.0.2 (Synaptosoft Inc, Decatur, GA, USA) as described earlier16. Discriminant function analysis with canonical discriminant functions and reclassification of group membership based on neuronal intrinsic properties were performed in SPSS.
Acute slice electrophysiology
We used litter-matched Ehmt1+/− and Ehmt1+/+ mice of adolescent age (postnatal day 21–24). Acute slices were prepared as described earlier16. In brief, animals were deeply anesthetized with isoflurane, then quickly decapitated. 350-µm-thick coronal slices were cut using a microtome (HM650V, Thermo Scientific) in ice-cold “cutting and storage” ACSF containing 87 mM NaCl, 11 mM glucose, 75 mM sucrose, 2.5 mM KCl, 1.25 mM NaH2PO4, 0.5 mM CaCl2, 7 mM MgCl2, and 26 mM NaHCO3, continuously oxygenated with 95% O2/5% CO2. Slices were incubated for 1 h at 32 °C, after which they were allowed to cool down to room temperature. The slices were then transferred to a recording setup as described above, and incubated in recording ACSF (124 mM NaCl, 10 mM glucose, 3 mM KCl, 1.25 mM NaH2PO4, 2 mM CaCl2, 1 mM MgCl2, and 26 mM NaHCO3) at 30 °C with added 100 mM Picrotoxin to block GABAergic transmission. A bipolar electrode (CE2C55, FHC) coupled to an SD9 stimulator (Grass Instruments, RI, USA) was inserted into layer 4 of the auditory cortex, and pyramidal cells were patched in layer 2/3 above the bipolar electrode (<200 µm lateral distance) using 3–6 MΩ borosilicate pipettes filled with a Cesium-based intracellular solution containing 115 mM CsMeSO3, 20 mM CsCl2, 10 mM HEPES, 2.5 mM MgCl2, 4 mM Na2ATP, 0.4 mM NaGTP, 10 mM Na-Phosphocreatine, 0.6 mM EGTA, and 5 mM QX-314. The cells were held in voltage-Clamp mode controlled by an SEC 05-LX amplifier (NPI, Tamm, Germany), low-pass filtered at 3 kHz and sampled at 20 kHz with a Power-1401 acquisition board and Signal software (CED, Cambridge, UK). Data were analyzed in Clampfit 10.7 (Molecular Devices). The AMPA trace was measured at −70 mV holding potential; stimulus strength was adjusted to 50–100 pA postsynaptic response, and 60 sweeps were averaged for the final trace. NMDA was measured at +40 mV holding potential. Cells were discarded if the measured AMPA response contained multiple peaks, indicating multisynaptic input, or if the averaged amplitude was below 25 pA. AMPA amplitudes were quantified as the peak amplitude at −70 mV holding voltage, and the NMDA amplitudes were quantified as the average of 5 ms of the trace 65 ms after the stimulus artifact.
For the animal experiments presented in this study, mice heterozygous for a targeted loss-of-function mutation in the Ehmt1 gene (Ehmt1+/− mice) and their WT littermates on C57BL/6 background were used, as previously described11. Animal experiments were conducted in conformity with the Animal Care Committee of the Radboud University Nijmegen Medical Centre, The Netherlands, and conform to the guidelines of the Dutch Council for Animal Care and the European Communities Council Directive 2010/63/EU.
The statistical analysis for all experiments was performed using GraphPad Prism 5 (GraphPad Software, Inc., CA, USA). We ensured normal distribution using a Kolmogorov–Smirnov normality test. To determine statistical significance for the different experimental conditions p values < 0.05 were considered to be significant. Statistical analysis between lines were performed with two-ways ANOVA and Post hoc Bonferroni correction. We analyzed significance between Control and Kleefstra groups by means of the Mann–Whitney U test. Data are presented as mean ± standard error of the mean (SE). Details about statistics are reported in Supplementary Data 1 (Excel file Statistics).
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
Geschwind, D. H. & Flint, J. Genetics and genomics of psychiatric disease. Science 349, 1489–1494 (2015).
De Rubeis, S. Synaptic, transcriptional and chromatin genes disrupted in autism. Nature 1–16 https://doi.org/10.1038/nature13772 (2014).
Kleefstra, T., Schenck, A., Kramer, J. & van Bokhoven, H. The genetics of cognitive epigenetics. Neuropharmacology https://doi.org/10.1016/j.neuropharm.2013.12.025 (2014).
Kochinke, K. et al. Systematic phenomics analysis deconvolutes genes mutated in intellectual disability into biologically coherent modules. Am. J. Hum. Genet. 98, 149–164 (2016).
Gabriele, M., Lopez Tobon, A., D'Agostino, G. & Testa, G. The chromatin basis of neurodevelopmental disorders: rethinking dysfunction along the molecular and temporal axes. Prog. Neuropsychopharmacol. Biol. Psychiatry https://doi.org/10.1016/j.pnpbp.2017.12.013 (2018).
Willsey, A. J. et al. The psychiatric cell map initiative: a convergent systems biological approach to illuminating key molecular pathways in neuropsychiatric disorders. Cell 174, 505–520 (2018).
Kleefstra, T. et al. Loss-of-function mutations in euchromatin histone methyl transferase 1 (EHMT1) cause the 9q34 subtelomeric deletion syndrome. Am. J. Hum. Genet. 79, 370–377 (2006).
Kleefstra, T. et al. Further clinical and molecular delineation of the 9q subtelomeric deletion syndrome supports a major contribution of EHMT1 haploinsufficiency to the core phenotype. J. Med Genet 46, 598–606 (2009).
Vermeulen, K. et al. The context of symptom measures: interpretation and clinical diagnosis of autism spectrum disorders in intellectual disabilities. J. Am. Acad. Child Adolesc. Psychiatry 56, 618–619 (2017).
Tachibana, M. et al. Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9. Genes Dev. 19, 815–826 (2005).
Balemans, M. C. M. et al. Hippocampal dysfunction in the Euchromatin histone methyltransferase 1 heterozygous knockout mouse model for Kleefstra syndrome. Hum. Mol. Genet. 22, 852–866 (2013).
Schaefer, A. et al. Control of cognition and adaptive behavior by the GLP/G9a epigenetic suppressor complex. Neuron 64, 678–691 (2009).
Kramer, J. M. et al. Epigenetic regulation of learning and memory by Drosophila EHMT/G9a. PLoS Biol. 9, e1000569 (2011).
Balemans, M. C. M. et al. Reduced exploration, increased anxiety, and altered social behavior: Autistic-like features of euchromatin histone methyltransferase 1 heterozygous knockout mice. Behav. Brain Res. 208, 47–55 (2010).
Balemans, M. C. M. et al. Reduced Euchromatin histone methyltransferase 1 causes developmental delay, hypotonia, and cranial abnormalities associated with increased bone gene expression in Kleefstra syndrome mice. Dev. Biol. 386, 395–407 (2014).
Benevento, M. et al. Histone methylation by the Kleefstra syndrome protein EHMT1 mediates homeostatic synaptic scaling. Neuron 1–16 https://doi.org/10.1016/j.neuron.2016.06.003 (2016).
Gupta-Agarwal, S. et al. G9a/GLP histone lysine dimethyltransferase complex activity in the hippocampus and the entorhinal cortex is required for gene activation and silencing during memory consolidation. J. Neurosci. 32, 5440–5453 (2012).
Sharma, M., Razali, N. B. & Sajikumar, S. Inhibition of G9a/GLP complex promotes long-term potentiation and synaptic tagging/capture in hippocampal CA1 pyramidal neurons. Cerebral Cortex 27, (3161–3171 (2017).
Takahashi, K. et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861–872 (2007).
Marchetto, M. C. et al. Altered proliferation and networks in neural cells derived from idiopathic autistic individuals. 1–16 https://doi.org/10.1038/mp.2016.95 (2016).
Linda, K., Fiuza, C. & Nadif Kasri, N. The promise of induced pluripotent stem cells for neurodevelopmental disorders. Prog. Neuropsychopharmacol. Biol. Psychiatry https://doi.org/10.1016/j.pnpbp.2017.11.009 (2017).
Willemsen, M. H. et al. Update on Kleefstra syndrome. Mol. Syndromol. 2, 202–212 (2012).
Willemsen, M. H. et al. Familial Kleefstra syndrome due to maternal somatic mosaicism for interstitial 9q34.3 microdeletions. Clin. Genet. 80, 31–38 (2011).
Zhang, Y. et al. Rapid single-step induction of functional neurons from human pluripotent stem cells. Neuron 78, 785–798 (2013).
Frega, M. et al. Rapid neuronal differentiation of induced pluripotent stem cells for measuring network activity on micro-electrode arrays. JoVE https://doi.org/10.3791/54900 (2017).
Selten, M., van Bokhoven, H. & Nadif Kasri, N. Inhibitory control of the excitatory/inhibitory balance in psychiatric disorders. F1000Res 7, 23 (2018).
Uhlhaas, P. J. & Singer, W. Neural synchrony in brain disorders: relevance for cognitive dysfunctions and pathophysiology. Neuron 52, 155–168 (2006).
Chailangkarn, T. et al. A human neurodevelopmental model for Williams syndrome. Nature 1–25 https://doi.org/10.1038/nature19067 (2016).
Martens, M. B. et al. Euchromatin histone methyltransferase 1 regulates cortical neuronal network development. Sci Rep 1–11 https://doi.org/10.1038/srep35756 (2016).
Suresh, J. et al. Network burst activity in hippocampal neuronal cultures: the role of synaptic and intrinsic currents. J. Neurophysiol. 115, 3073–3089 (2016).
Odawara, A., Katoh, H., Matsuda, N. & Suzuki, I. Physiological maturation and drug responses of human induced pluripotent stem cell-derived cortical neuronal networks in long- term culture. Sci. Rep. 1–14 https://doi.org/10.1038/srep26181 (2016).
Lee, H.-K. Ca-permeable AMPA receptors in homeostatic synaptic plasticity. Front. Mol. Neurosci. 5, 17 (2012).
Turrigiano, G. G., Leslie, K. R., Desai, N. S., Rutherford, L. C. & Nelson, S. B. Activity-dependent scaling of quantal amplitude in neocortical neurons. Nature 391, 892–896 (1998).
Rundfeldt, C. The new anticonvulsant retigabine (D-23129) acts as an opener of K+ channels in neuronal cells. Eur. J. Pharmacol. 336, 243–249 (1997).
Mierau, S. B., Patrizi, A., Hensch, T. K. & Fagiolini, M. Cell-specific regulation of N-methyl-D-aspartate receptor maturation by Mecp2 in cortical circuits. Biol. Psychiatry 79, 746–754 (2016).
Song, X. et al. Mechanism of NMDA receptor channel block by MK-801 and memantine. Nature 556, 515–519 (2018).
Frega, M. et al. Distinct Pathogenic Genes Causing Intellectual Disability and Autism Exhibit Overlapping Effects on Neuronal Network Development. 1–41 https://doi.org/10.1101/408252 (2018).
Rojas, D. C. The role of glutamate and its receptors in autism and the use of glutamate receptor antagonists in treatment. J. Neural Transm. 121, 891–905 (2014).
Lee, E.-J., Choi, S. Y. & Kim, E. ScienceDirect NMDA receptor dysfunction in autism spectrum disorders. Curr. Opin. Pharm. 20, 8–13 (2015).
Purcell, A. E., Jeon, O. H., Zimmerman, A. W., Blue, M. E. & Pevsner, J. Postmortem brain abnormalities of the glutamate neurotransmitter system in autism. Neurology 57, 1618–1628 (2001).
Rinaldi, T., Kulangara, K., Antoniello, K. & Markram, H. Elevated NMDA receptor levels and enhanced postsynaptic long-term potentiation induced by prenatal exposure to valproic acid. Proc. Natl Acad. Sci. USA 104, 13501–13506 (2007).
Guy, J., Hendrich, B., Holmes, M., Martin, J. E. & Bird, A. A mouse Mecp2-null mutation causes neurological symptoms that mimic Rett syndrome. Nat. Genet. 27, 322–326 (2001).
Ip, J. P. K., Mellios, N. & Sur, M. Rett syndrome: insights into genetic, molecular and circuit mechanisms. Nat. Rev. Neurosci. 1–15 https://doi.org/10.1038/s41583-018-0006-3 (2018).
Blue, M. E. et al. Temporal and regional alterations in NMDA receptor expression in Mecp2-null mice. Anat. Rec. 294, 1624–1634 (2011).
Chung, C. et al. Early correction of N-methyl-D-aspartate receptor function improves autistic-like social behaviors in adult Shank2-/- mice. BPS 1–10 https://doi.org/10.1016/j.biopsych.2018.09.025 (2018).
Patrizi, A. et al. Chronic administration of the N-methyl-D-aspartate receptor antagonist ketamine improves Rett syndrome phenotype. Biol. Psychiatry 79, 755–764 (2016).
Tu, S. et al. NitroSynapsin therapy for a mouse MEF2C haploinsufficiency model of human autism. Nat. Commun. 8, 1488 (2017).
Tang, S. et al. Altered NMDAR signaling underlies autistic-like features in mouse models of CDKL5 deficiency disorder. Nat. Commun. 10, 2655 (2019).
Gupta-Agarwal, S., Jarome, T. J., Fernandez, J. & Lubin, F. D. NMDA receptor- and ERK-dependent histone methylation changes in the lateral amygdala bidirectionally regulate fear memory formation. Learn. Mem. 21, 351–362 (2014).
Iacono, G. et al. Increased H3K9 methylation and impaired expression of Protocadherins are associated with the cognitive dysfunctions of the Kleefstra syndrome. Nucleic Acids Res. 78, 321–16 (2018).
Bai, G., Zhuang, Z., Liu, A., Chai, Y. & Hoffman, P. W. The role of the RE1 element in activation of the NR1 promoter during neuronal differentiation. J. Neurochem. 86, 992–1005 (2003).
Rodenas-Ruano, A., Chávez, A. E., Cossio, M. J., Castillo, P. E. & Zukin, R. S. REST-dependent epigenetic remodeling promotes the developmental switch in synaptic NMDA receptors. Nat. Neurosci. 15, 1382–1390 (2012).
Mukherjee, S., Brulet, R., Zhang, L. & Hsieh, J. REST regulation of gene networks in adult neural stem cells. Nat. Commun. 7, 13360 (2016).
Schratt, G. M., Nigh, E. A., Chen, W. G., Hu, L. & Greenberg, M. E. BDNF regulates the translation of a select group of mRNAs by a mammalian target of rapamycin-phosphatidylinositol 3-kinase-dependent pathway during neuronal development. J. Neurosci. 24, 7366–7377 (2004).
Katz, D. M., Menniti, F. S. & Mather, R. J. N-methyl-D-aspartate receptors, ketamine, and Rett syndrome: something special on the road to treatments? Biol. Psychiatry 79, 710–712 (2016).
Bello, O., Blair, K., Chapleau, C. & Larimore, J. L. Is memantine a potential therapeutic for Rett syndrome? Front. Neurosci. 7, 245 (2013).
Joshi, G. et al. A prospective open-label trial of memantine hydrochloride for the treatment of social deficits in intellectually capable adults with autism spectrum disorder. J. Clin. Psychopharmacol. 36, 262–271 (2016).
Bjornsson, H. T. et al. Histone deacetylase inhibition rescues structural and functional brain deficits in a mouse model of Kabuki syndrome. Sci. Transl. Med. 6, 256ra135 (2014).
Nagy, J. et al. Altered neurite morphology and cholinergic function of induced pluripotent stem cell-derived neurons from a patient with Kleefstra syndrome and autism. Transl. Psychiatry 7, e1179 (2017).
Mandegar, M. A. et al. CRISPR interference efficiently induces specific and reversible gene silencing in human iPSCs. Cell Stem Cell 18, 541–553 (2016).
Livak, K. J. & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25, 402–408 (2001).
Bologna, L. L. et al. Investigating neuronal activity by SPYCODE multi-channel data analyzer. Neural Netw. 23, 685–697 (2010).
We thank Dr. J. Ladewig for providing the NGN2 lentiviral construct. Funding: This work was supported by grants from: the Netherlands Organization for Scientific Research, open ALW ALW2PJ/13082 (to H.v.B., N.N.K., H.Z., T.K., and D.S.); grant 012.200.001 (to N.N.K.); the Netherlands Organization for Health Research and Development ZonMw grant 91718310 (to T.K.), 91217055 (to. H.v.B. and N.N.K.), SFARI grant 610264 (to N.N.K.) and the Jerome Lejeune Foundation (to H.v.B). GGA is supported by a postdoctoral research grant from TÜBITAK (1059B191600426). I.v.d.W is supported by the MINDED project, EU H2020 Marie Skłodowska-Curie (grant agreement No 754490).
The authors declare no competing interests.
Peer review information Nature Communications thanks Maria Passafaro and the other, anonymous, reviewer(s) for their contribution to the peer review of this work. Peer reviewer reports are available.
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Frega, M., Linda, K., Keller, J.M. et al. Neuronal network dysfunction in a model for Kleefstra syndrome mediated by enhanced NMDAR signaling. Nat Commun 10, 4928 (2019). https://doi.org/10.1038/s41467-019-12947-3
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