Abstract
Oxidation of indoles is a fundamental organic transformation to deliver a variety of synthetically and pharmaceutically valuable nitrogen-containing compounds. Prior methods require the use of either organic oxidants (meta-chloroperoxybenzoic acid, N-bromosuccinimide, t-BuOCl) or stoichiometric toxic transition metals [Pb(OAc)4, OsO4, CrO3], which produced oxidant-derived by-products that are harmful to human health, pollute the environment and entail immediate purification. A general catalysis protocol using safer oxidants (H2O2, oxone, O2) is highly desirable. Herein, we report a unified, efficient halide catalysis for three oxidation reactions of indoles using oxone as the terminal oxidant, namely oxidative rearrangement of tetrahydro-β-carbolines, indole oxidation to 2-oxindoles, and Witkop oxidation. This halide catalysis protocol represents a general, green oxidation method and is expected to be used widely due to several advantageous aspects including waste prevention, less hazardous chemical synthesis, and sustainable halide catalysis.
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Introduction
Chemical oxidation of indoles is a fundamental organic transformation to deliver a diverse array of versatile nitrogen-containing compounds, in particular 2-oxindoles, which have been used widely in organic synthesis and drug discovery1,2,3,4. The electron-rich property of indoles allows the oxidation to occur under many oxidation conditions. However, a mixture of oxidation products is usually observed due to the competing oxidation of nitrogen, C2 and C3, as well as potential rearrangement and over-oxidation (Fig. 1a)5,6,7. The challenging chemo-selectivity and regio-selectivity requires not only a site-selective oxidant but also suitable substitutions at C2 and/or C3, as well as the protecting group on the nitrogen. Therefore, it is not surprising that only a small number of oxidants have been identified for only one or two of the three major types of the indole oxidation (Fig. 1a): (i) oxidative rearrangement of tetrahydro-β-carbolines to spirooxindoles8,9,10,11,12,13,14, (ii) oxidation of C3-substituted indoles to 2-oxindoles15, and (iii) oxidative cleavage of C2,C3-disubstituted indoles to 2-keto acetanilides (Witkop oxidation)16,17. Although these oxidants under the optimized conditions could solve the chemo-selectivity and regio-selectivity with high yields, their environmental and/or health impacts were not addressed, which is contrary to the rising concept and awareness of Green Chemistry.
Oxone (KHSO5-1/2KHSO4-1/2K2SO4, MW 307) has been widely used as a green, cheap, and safe oxidant because it generates K2SO4 as the only oxidant-derived byproduct18. Though inferior to oxygen (O2) and hydrogen peroxide (H2O2) in terms of atom economy19, oxone has not only admirable bench-stability for storage and transportation but also exceptional reactivity towards halide (e.g., bromide and chloride) oxidation under weakly acidic/basic or even neutral conditions which is advantageous over related halide oxidation with hydrogen peroxide or oxygen under either strong acid (HCl or HBr) or transition metal catalysis20. We previously exploited its unique reactivity towards halide oxidation and have established several mild green oxone-halide protocols to replace the corresponding NXS-mediated oxidations21,22. For example, we found that oxone-halide could be used to replace NBS (or NCS) for oxidative halocyclization of tryptamine and tryptophol derivative (Fig. 1b)22. Inspired primarily by this work, we envision that in the absence of a tethered nucleophile the indolenine (II) can react with water (part of the solvent) to generate 3-halo-2-hydroxy indoline (II → III, Fig. 1c), which might undergo semi-pinacol rearrangement23 to provide spirooxindoles or 2-oxindoles. Alternatively, addition of potassium peroxymonosulfate (from excess of oxone) to indolenine (II) may generate hydroperoxysulfate intermediate IV (III → IV)16,17. Subsequent substitution of the halide with water triggers the C2-C3 bond cleavage of V to afford 2-keto acetanilides. In both scenarios, the halide is released and can be re-oxidized by oxone to generate the halogenating species. Therefore, halide is theoretically a catalyst for the oxone oxidation of indoles. This article presents the verification and implementation of this hypothesis, leading to the development of a unified green protocol for the oxidation of indoles to spirooxindoles, 2-oxindoles and 2-keto acetanilides (Fig. 1c). Our protocol (oxone-halide) can eliminate not only the use of hazardous oxidants (e.g., Pb(OAc)4, CrO3, OsO4, t-BuOCl, NBS, and m-CPBA, etc) but also the production of organic byproducts or toxic heavy metals derived from oxidants to minimize the environmental and health impact of the indole oxidation.
Results
Oxidative rearrangement of tetrahydro-β-carbolines
The tricyclic spirooxindole core, in particular the spiro[pyrrolidine-3,3’-oxindole], is a privileged scaffold featured in a variety of medicinal agents (anti-tumor, anti-microbial, anti-viral, and anti-malarial, etc) and bioactive natural alkaloids (eg., spirotryprostatins, rhynchophylline, alstonisine, horsfiline)24,25 (Fig. 2a). Oxidative rearrangement of tetrahydro-β-carbolines (THCs) to spirooxindoles was proposed as a biosynthetic process to account for the production of these metabolites and biogenesis connection with THC-type corynanthe alkaloids26,27,28. It has been successfully mimicked as a key transformation in the total synthesis of many spirooxindole alkaloids and thus oxidative rearrangement becomes a major approach for the synthesis of spirooxindoles29. However, the four identified stoichiometric oxidants: Pb(OAc)412, OsO430, t-BuOCl8,9,11,12, and N-bromosuccinimide (NBS)31 for the oxidative rearrangement are either unsafe to use or environmentally unfriendly. Pb(OAc)4 and OsO4 are extremely toxic heavy metal-based oxidants that pose a significant threat to the human health and environment; while t-BuOCl is an unstable, flammable, harmful liquid that usually requires fresh in-house preparation and appropriate titration32. In addition, t-BuOCl required a subsequent acid treatment to complete the rearrangement (II → III, Fig. 2c). NBS was usually used in an acidic condition (AcOH–H2O) and inevitably produced the corresponding stoichiometric succinimide byproduct that required immediate elimination by column purification. Therefore, a green catalytic protocol is highly desirable.
In continuation of our interest in developing green oxone-halide protocols to replace N-halosuccinimides (NXS) and related halogenating reagents (e.g., Cl2, Br2, t-BuOCl, etc), we set out to explore the oxidative rearrangement of THCs using oxone-halide as an green alternative (Fig. 2b) to the widely used NBS and t-BuOCl conditions. We believed such alternative was highly viable from the mechanistic perspective (Fig. 2c, d). The oxidative rearrangement of THCs involves a three-step sequence: oxidative halogenation, addition of water, and semi-pinacol rearrangement (Fig. 2c). We envisioned that oxone-halide (e.g., bromide) could deliver the reactive halogenating agent for the first step: oxidative halogenation. Small amount of water necessary for dissolving oxone as a co-solvent might add to β-bromo indolenine (II); while the halide released in the semi-pinacol rearrangement of III could be re-oxidized by oxone to re-generate the halogenating agent for the next-cycle THC oxidation (Fig. 2d). In principle, catalytic amount of halide (e.g., KBr) in combination of stoichiometric oxone could be used for replacement of t-BuOCl and NBS to achieve the goal of a green chemistry approach for the oxidative rearrangement of THCs to spirooxindoles.
To verify our hypothesis, we used THC 1a as our model compound to examine its oxidative rearrangement under various conditions (Table 1). We quickly found that the combination of oxone (1.2 eq) and KBr (5 mol%) in both THF/H2O (v/v = 1:1, 3:1, or 10:1) and MeCN/H2O (v/v = 1:1, 3:1, or 10:1) effected the oxidative rearrangement within 4 h in excellent yields (84-93%) (Table 1, entries 1 and 2). As compared to NBS-AcOH (83% yield) and t-BuOCl-AcOH (79% yield), our protocol under optimal condition was higher yielding (93%). Other halides including tetrabutyl ammonium bromide (TBAB) (Table 1, entry 3), tetrabutyl ammonium iodide (TBAI) (Table 1, entry 4), tetrabutyl ammonium chloride (TBAC) (Table 1, entry 5), KI (Table 1, entry 6), KCl (Table 1, entry 7), NH4Cl (Table 1, entry 8) and NaCl (Table 1, entry 9), were also evaluated as the halide catalyst. We found that only TBAB was a competent halide catalyst without added advantage in terms of reaction time and yield. In the absence of halide (Table 1, entry 10), no rearranged product was observed in 24 h, which suggested that halide was the active catalyst for the oxidative rearrangement. In addition, other terminal oxidants including H2O2, K2S2O8, NaOCl, NaClO2, and t-BuOOH were examined but they were inferior to oxone (entries 11–15) because they were either unable to oxidize bromide (H2O2 and t-BuOOH, Table 1, entries 11 and 15) or unselective for oxidation of bromide and indole (K2S2O8, NaOCl, and NaClO2, Table 1, entries 12–14).
Next, we set out to examine the substrate scope (Table 2). It should be noted that, to the best of our knowledge, the substrate scope of this biomimetic oxidative rearrangement has not been studied systematically despite the fact that it was often used in the biomimetic total synthesis of spirooxindole alkaloids29. We first investigated the electronic effect of the protecting group (N–R1 and N–R3) on the nitrogen (Table 2, entries 2a‒2j). It was found that electron-donating group (EDG) including hydrogen, alkyl, and benzyl on the indole nitrogen (R1 = H, alkyl, Bn) was essential to the success of oxidative rearrangement (Table 2, entries 2a‒2c). Interestingly, electron-withdrawing group (EWG, e.g., Ac, Ts, and Boc) on the indole nitrogen (N–R1) resulted in lower conversion (20–50%) and loss of EWG (2a was obtained instead of the expected 2d). The high chemoselectivity of indole oxidation via halide catalysis is hinged on that in situ generated halenium ion (c.f., Br+) as a catalyst reacts only with electron-rich indole (C2=C3) to form the corresponding indole halonium intermediate (I, Fig. 1c).
An electron-withdrawing group on the indole nitrogen (R1 = Ts, Boc) will substantially decrease the electron-density of indoles and consequently suppress the halenium-catalyzed indole oxidation, which is consistent with the result of 2d with 0% yield (R1 = Ts or Boc) in Table 2. On the other hand, the electronic property of protecting group on the piperidine (N-R3) is less significant to the electron density of indoles (not a conjugate system) and less influential to their oxidation under the halide catalysis, which is consistent with the higher yield (90–99%) with N-EWG (Table 2, entries 2e‒2h: N-Boc, N-Ts, N-Cbz, and N-Ac). The low conversion (10–20%) for EDG (Table 2, entries 2i‒2j: N–Me and N–Bn) under neutral condition might be attributed to preferentially oxidize the tertiary amine. It was later found that an acidic medium (THF/AcOH/H2O = 1:1:1) could substantially improve the conversion (>90%) and yield (60-74%). The lower isolated yield for electron-donating N-R3 (Table 2, entries 2i, 2j, 2w, 2x, and 2y) products might be due to the difficult isolation/purification of tertiary amine products from our acidic reaction medium. Two examples with electron-donating substituents (Me and OMe) on the THCs were selected to probe the possibly competing bromination on the benzene ring of THCs under our halogenating condition. Fortunately, the oxidative rearrangement occurred smoothly under our optimized condition (Table 2, entries 2k and 2l) and no aromatic bromination was detected. This finding was important to relevant total synthesis because such EDG substituents are found in a number of natural products (spirotryprostatin A, horsfiline and elacomine). Next, a variety of C1-substituted THCs were examined for their oxidative rearrangement (Table 2, entries 2m‒2y). Except for C1-aryl THC (Table 2, entry 2v, 0%) that resulted in unexpected oxidative C1–N3 cleavage (see Supplementary Figs. 92 and 93 for the structure of this side product and possible mechanisms), all C1-substituted THCs with various functional groups (alkene, CN, OBn, alkyne, and CO2Et) underwent smooth oxidative rearrangement to give the spirooxindole products in good to excellent yields with diastereoselectivity ranging from 1.5:1 to 3.8:1. Most of these diastereomers could be separated easily by column chromatography on silica gel and their relative stereochemistry was proposed according to the relative configuration of 2o (3R*/4S*) and 2o' (3R*/4R*), which were confirmed by X-ray diffraction analysis. It was to our surprise that 2u was obtained in 80% yield as a single diastereomer (dr 20:1, 3R*/4S*). This remarkable high diastereoselectivity was in sharp contrast to those of tryptophan-derived THC 2z (dr 4:1). Interestingly, we found that C3-ester substitution enhanced the stereocontrol of C1-alkyl on the spirocenter from 1.5/1–3.8/1 (Table 2, entries 2m/m'–2t/t') to 7/1–20/1 (Table 2, entries 2ac and 2ab). Another unexpected observation was that electron-donating group on the piperidine nitrogen (R3) appeared to reverse such diastereoselectivity, leading to isolation of the major products (Table 2, entries 2w and 2y) with different relative stereochemistry (3R*/4R*). The intriguing diastereoselectivity was not documented in the literature and our finding would be instrumental to the design and synthesis of spirooxindoles from THCs.
To showcase the utility of this protocol (Fig. 3), we achieved the total synthesis of two popularly targeted spirooxindole natural products (±)-coerulescine (1.2 g, 2i) and (±)-horsfiline (3) from THC 1a (Fig. 3a)31,33,34,35,36,37. Reduction of THC 1a with LiAlH4 and oxidative rearrangement of the resulting THC 1i using our oxone-KBr under acidic condition (THF/AcOH/H2O = 1:1:1) furnished (±)-coerulescine (2i) with 1.2 g in two steps (overall yield: 39%). If the oxidative rearrangement of THC 1i was carried out with stoichiometric KBr and 2.4 equivalent of oxone, sequential one-pot oxidative rearrangement and bromination occurred to provide C5-bromo spirooxindole 2ad in 41% yield, which could be used for CuI-catalyzed Ullmann ether synthesis to furnish (±)-horsfiline (3) in 60% yield. Notably, this protocol allowed a one-pot sequential oxidative rearrangement and dibromination (1a → 2ae, 86% yield) when 2.1 equivalent of KBr and 3.6 equivalent of oxone were employed. This offered a compelling flexibility to access to a wide variety of spirooxindoles. Finally, we applied this protocol for the biomimetic oxidative rearrangement of natural alkaloid yohimbine and obtained the corresponding yohimbine oxindole 4 (Fig. 3b) in 56% yield, which apparently was superior to the reported three-step method38 with only 38% overall yield.
To further extend this protocol, we were interested in the rarely-explored oxidative rearrangement of 1,3,4,9-tetrahydropyrano[3,4-b]indoles39,40 (THPIs, 5a–5e) to the oxa-spirooxindoles (Fig. 3d) because (i) oxa-spirooxindole is the structural core in many pharmaceutically important molecules41,42 (Fig. 3c) and (ii) there are only a few synthetic methods43,44. To our delight, without further condition optimization all five THPIs underwent the expected oxidative rearrangement to provide the oxa-spirooxindoles 6a‒6d in good to excellent yields, which constitutes the second examples of oxidative rearrangement of THPIs45. Remarkably, the diastereoselectivity (3R*/4R*) was unusually high and only a single diastereomer was isolated. The relative stereochemistry of 6a, 6c, and 6e was confirmed by X-ray diffraction. Notably, oxa-spirooxindole 6a possessed the same relative configuration as coixspirolactam C46,47 and could be used as a direct precursor for the synthesis of coixspirolactam C45.
Oxidation of indoles to 2-oxindoles
The success of the green approach for the oxidative rearrangement of THCs/THPIs to (oxa-)spirooxindoles prompted us to explore the possibility of the oxone-halide oxidation of the simpler C3-substituted indoles to 2-oxindoles. 2-Cxindoles are not only important structural motifs in a number of biologically active alkaloid natural products and pharmaceutical molecules47 but also frequently used as the synthetic building blocks in the synthesis of natural alkaloids and as the platform for development of synthetic methodologies48. As shown in Fig. 4, the prior methods for direct oxidation of indoles to 2-oxindoles employed usually NBS5 or m-CPBA49 as the stoichiometric oxidant, even though electrophilic fluorinating agents such as selectfluor50 and Togni’s reagent15 were found to be ideal oxidants for some specific indoles that suffered from low yields when using NBS and m-CPBA. The DMSO-HCl (37%) condition51 was often limited to the oxidation of simple indoles without acid-labile functional groups. Apparently, there lacks of a green and efficient method for the indole oxidation to 2-oxindoles. We believed that the green oxone-halide oxidation system could be applicable to this case.
We chose 3-methylindole (skatole, 7a) as our model compound to examine the direct oxidation of indoles to 2-oxindoles. After quick screening of various solvents (Table 3, entries 1–9), three solvent systems: THF/H2O (20:1), MeCN/H2O (20:1), and t-BuOH/H2O (20:1), were identified to be an excellent reaction medium. We selected t-BuOH/H2O (20:1) for the best yield (91%) of 3-methyloxindole (8a) from the skatole oxidation. Notably, KBr was essential (Table 3, entry 13) and outperformed the corresponding KCl (58%) and KI (0%) (Table 3, entries 10 and 11), while the higher water ratio in the mixed solvent system only slightly lowered the yield of oxidation (Table 3, entry 12). It was noted that our oxone-KBr protocol was more efficient than other methods (Togni’s reagent: 77%; NBS: 26–83%; CH3CO3H: 14%) for skatole oxidation.
This optimized condition was applied to oxidation of a variety of C3-substituted indoles (Fig. 5a). Our examination of the substrate scope led to some interesting findings. First of all, the electronic property of the protecting group on the indole nitrogen has a dominant influence on the oxidation: electron-donating groups including methyl, benzyl, allyl (R1 = Me, Bn, Allyl) were favorable (8b‒8c, 90–92%), while electron-withdrawing group (e.g., R1 = Ts or Boc) completely suppressed the oxidation (8d', 0%). Secondly, the electronic properties of C3 substituents (R2) is critical to the success of oxidation: C3-alkyl (electron-donating) indoles gave high yields while the parent indole, C3-phenylindole and C3-trifluoromethyl failed to deliver the corresponding 2-oxindoles (8a', 8a'', and 8a''' 0%). This finding was consistent with the observation that indole oxidation via halide catalysis required electron-rich indoles. The parent indole suffered from poor chemo-selectivity and regio-selectivity and gave a complex mixture, which was observed by m-CPBA. Thirdly, substitution at C5 and C7 of indoles (R3 = 7-Me, 5-Br, and 5-OMe) has little effect on the oxidation (8e‒8g, 88–91%). Moreover, our oxone-KBr protocol was applicable to tryptamines (8j–8p), tryptophans (8q), tryptophols (8w–8z), and their derivatives (8r–8v). In addition, the esters (8q‒8s), carbamate (8j, 8q), sulfonamides (8k‒8l, 8n‒8p), cyanide (8t‒8v), and even free alcohol (8w‒8z) were tolerated in this mild oxidation condition, which outperformed prior methods regarding the functional group tolerance and efficiency.
To showcase the scalability and utility of this oxone-KBr oxidation process (Fig. 5b), the catalytic oxidation of 7b and 7h on 2.0 mmol (2.62 g and 4.15 g, respectively) scales was carried out to provide the desire 2-oxindoles 8b and 8h in the excellent yield of 91% and 88%, respectively, which were used for the concise unified total syntheses of desoxyeseroline, physovenol methyl ether, and esermethole48,52,53,54. The availability of 2-oxindoles 8b and 8h with gram quantities enabled alkylation with two-carbon bromides55 to provide the 3,3-disubstituted 2-oxindoles (9a–9d). Reductive cyclization56 was employed for the construction of the key tricyclic hexahydropyrroloindolines (HPIs, 10c and 10d) and tetrahydrofuroindolines (TFIs, 10a and 10b). N-Methylation of the resulting hexahydropyrroloindoline furnished desoxyeseroline (10c) in 70% yield (46% overall yield for three steps). CuI-catalyzed Ulmann coupling of aryl bromide with NaOMe completed the synthesis of physovenol methyl ether (11a) and esermethole (11b) in 72% and 74%, respectively. Notably, physovenol methyl ether and esermethole were the 2-step precursor of respective physovenine and physostigmine57.
In order to shed some light on the oxidation mechanism, we performed a small set of controlled experiments (Fig. 5c). 2-Deuterated 3-methyindole(D-7a, 72% D) was prepared and used for oxone-KBr oxidation. 49% Deuterium incorporation at C3 was observed in D-8a (90% yield). When D2O was used as a co-solvent, 85% deuterium at C3 was observed for the oxidation of un-deuterated substrate 7a. This seemingly contradictory result was attributed to the keto-enol tautomerism of 2-oxindole 8a under either neutral condition (THF/D2O, 14%D) or our standard condition (21%D). C2-Bromoindole (7aa) was not the intermediate for our oxone-KBr oxidation because it failed under our condition to provide 2-oxindole 8a. In addition, no 2-oxindole 8a was observed from the oxidation of 2-methylindole (7ab), which suggested that C3-alkyl substitution stabilized the developing positive charge at C3 in the course of bromide departure. All these results supported the proposed mechanism (Fig. 1c) that involved semi-pinacol rearrangement to provide the 2-oxindoles. However, we could not exclude the possible H–Br elimination over semi-pinacol rearrangement to afford 2-oxindoles when C2 was unsubstituted.
Witkop oxidation of indoles to 2-keto acetanilides
Oxidative cleavage of aromatic rings occurs frequently in Nature58. In particular, the enzymatic oxidation of tryptophan to N-formylkynurenine is not only a major metabolic pathway of tryptophan but also the first key step of the biosynthesis of coenzyme NAD59. The first chemical process of the corresponding oxidative cleavage of the C2–C3 double bond of indoles was reported in 1951 by Witkop16,17 using Pt/O2 oxidation (Fig. 6b). Subsequently, various oxidants including peracids (m-CPBA), periodic acid (NaIO4), chromic acid, ozone and singlet oxygen, were identified for Witkop oxidation16,17,60,61,62,63,64. Notably, Winterfeldt65 found that NaH/O2 and KOtBu/O2 could effect both Witkop oxidation and Camps cyclization to provide quinolones, which widely exist in many marketed drugs and bioactive molecules66 (Fig. 6a). The importance of Witkop oxidation of indoles to 2-keto-acetanilides for the Camps cyclization to quinolones and for the commercial preparation of benzodiazepines67,68 (drugs for treatment of insomnia and anxiety) aroused our interest in developing a green oxidation protocol for Witkop oxidation using the oxone-halide system (Fig.6c).
We chose 2,3-dimethyl indole (12a) as the model compound to examine the viability of Witkop oxidation with the oxone-halide system. Surprisingly, the optimized conditions developed for oxone-halide oxidation of indoles to spirooxindoles and 2-oxindoles afforded only 14–34% yield of fragmentation product 13a (Table 4, entries 1–3). A large-scale screening of solvents (Table 4, entries 4–6) and halides (Table 4, entries 7–9) enabled us to identify a clean and efficient system: oxone-KCl in HFIP/H2O (10:1) (Table 4, entry 8), which could deliver the desired Witkop product 13a in 74%. It was noted that the reaction time should be extended to 24 h, which was much longer than the time required for the oxidation of indoles to spirooxindoles and 2-oxindoles (1–4 h).
The success of Witkop oxidation with oxone-halide system in our model study prompted us to investigate the substrate scope (Fig. 6c). It was found electron-withdrawing group on the indole nitrogen (N-Ac or N-Boc) could not allow for the Witkop oxidation with oxone-KCl. One of our major findings in the course of expanding substrate scope was that C2 substitution (R2 ≠ H) was necessary for the oxidative cleavage (12a–12o) as the C2-unsubstituted indole 7w only resulted in 2-oxindole 8w (~40%). Although C2,C3-disubstituted indoles were excellent substrates for Witkop oxidation with oxone-halide in HFIP/H2O system, C3-substitution was not critical (e.g., 12f and 12g: R3 = H). In the latter case, it should be noted that the oxone-KCl oxidation led to isolation of carboxylic acids 13f and 13g, instead of the expected aldehydes. Another interesting observation was that oxone-KCl oxidation of C3-aldehydic indole 12i provided the unexpected carboxylic acid 13f, which might be arisen from an oxidation sequence involving C2–C3 cleavage, aldehyde oxidation, and oxidative decarboxylation. We recognized that our oxone-KCl in HFIP/H2O was too acidic for tert-butyldimethylsilyl ethers (12i and 12k), leading to desilylated Witkop oxidation products (13j and 13k). Fortunately, the free alcohol survived from this oxidation condition. It was intriguing to observe that 1,2,3,4-tetrahydrocarbazoles (12m and 12n) could undergo smoothly oxidative C2–C3 cleavage, while the corresponding tetrahydro-β-carboline 1a only resulted in oxidative rearrangement under the identical condition (oxone-KCl in HFIP/H2O). At this stage, without further experimentation we could not provide a good explanation for this puzzling result. Finally, we examined the Witkop oxidation of indoles with CF3 substitution at C2/C3 (12o/12p) and found that C2–CF3 indole could deliver the desired Witkop product 13o in 46% while 12p decomposed under the condition. Nevertheless, the property of substituents did play a decisive role in the oxidation of indoles to different products and we believed that our result would support the hypothetic mechanism of Witkop oxidation in Fig. 1c.
Discussion
We have developed a general halide catalysis for green oxidation of indoles to spirooxindoles, 2-oxindoles, 2-keto acetanilides. Our study demonstrated that oxone-halide could replace other organic halogenating agents (NBS, NCS, t-BuOCl etc) or peracids (m-CPBA) in different types of oxidation of indoles, and thus eliminate the production of toxic organic byproducts derived from oxidants. As compared to prior methods, this protocol was usually more efficient partly due to the in situ generated halenium ion (X+) catalyst that has the appropriate concentration and reactivity towards the C2–C3 double bond of indoles and thus significantly suppressed other competing oxidations/rearrangements. In addition, no need to protect the indole nitrogen was advantageous since most previous methods required to mask the indole nitrogen with electron-withdrawing groups (e.g., N-Ts, N-Boc, N-Ac etc) for better chemo-selectivity and regio-selectivity. Achieving this oxone-halide oxidation of indoles was a milestone in the indole oxidation for its low-cost, safe/simple operation (open flask), and most importantly its greenness in several aspects of the 12 Green Chemistry Principles including (1) preventing waste, (2) less hazardous chemical synthesis, (3) safer chemicals, and (4) using catalysis. We believed that this oxone-halide system might be used for other types of indole oxidation that were not explored in this article. It is our expectation that this oxone-halide protocol for the indole oxidation will find wide applications in academia (organic synthesis) and industrial (pharmaceutical) communities.
Methods
Oxidative rearrangement of tetrahydro-β-carbolines
To a stirred solution of THC (1.0 eq) and KBr (5–10 mol%) in MeCN/H2O (10/1, 0.1 M) or in THF/H2O/AcOH(1/1/1, 0.1 M) at 0 °C was added oxone (1.2 eq, MW = 307) in one batch. The resulting solution was allowed to warm to rt, and stirred for 1–16 h. After the reaction was completed as determined by TLC analysis, the reaction was quenched by addition of aq. sat. NaHCO3 and aq. sat. Na2SO3 and then diluted with EtOAc. The organic fractions were collected, and the aqueous phase was extracted with EtOAc three times. The combined organic fractions were washed with brine, dried over Na2SO4, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography to give spirooxindoles.
Oxidation of C3-substituted indole to 2-oxindole
To a solution of C3-substituted indole (1.0 eq) and KBr (10 mol%) in t-BuOH/H2O (20/1, 0.1 M) at rt was added oxone (1.2 eq, MW = 307), and was stirred for 1–4 h. The reaction was quenched by addition of aq. sat. Na2SO3 and then diluted with EtOAc. The organic fractions were collected and the aqueous phase was extracted with EtOAc three times. The combined organic franctions were washed with brine, dried over Na2SO4, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography to give the 2-oxindoles.
Witkop oxidation of indole to 2-keto acetanilide
To a solution of indole (12a–12n, 1.0 eq) and KCl (10 mol%, 0.01 M) in HFIP/H2O (10/1, 0.1 M) at rt was added oxone (1.2 eq, MW = 307) in one batch. The resulting solution was stirred at room temperature for 24-h and then diluted with EtOAc. The reaction mixture passed through a short pad of silica gel and washed with EtOAc. The resulting EtOAc/HFIP solution was concentrated under reduced pressure and the residue was purified by flash column chromatography to give 2-keto acetanilides.
Data availability
Experimental procedures and characterization data are available within this article and its Supplementary Information. Data are also available from the corresponding author on request. The X-ray crystallographic coordinates for structures reported in this study have been deposited at the Cambridge Crystallographic Data Center (CCDC), under deposition numbers 1935503, 1935504, 1935506, 1935507, and 1935508. These data can be obtained free of charge from The Cambridge Crystallographic Data Center via www.ccdc.cam.ac.uk/data_request/cif.
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Acknowledgements
This research was financially supported by Research Grant Council of Hong Kong (16311716, 16303617, 16304618) and National Natural Science Foundation of China (21772167). Dr. J.X. also acknowledged the Doctor Start-up Fund ([2018]28) and the Guizhou Province First-Class Disciplines Project (Yiliu Xueke Jianshe Xiangmu-GNYL[2017]008) from Guizhou University of Traditional Chinese Medicine (China).
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J.X. and L.L. performed the experiments. H.Z. prepared some related substrates. Y.R.C. participated in discussing part of the experiments. R.T. conceptualized and directed the project, and drafted the paper with the assistance from all co-authors.
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Xu, J., Liang, L., Zheng, H. et al. Green oxidation of indoles using halide catalysis. Nat Commun 10, 4754 (2019). https://doi.org/10.1038/s41467-019-12768-4
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DOI: https://doi.org/10.1038/s41467-019-12768-4
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