Serum resistance is a poorly understood but common trait of some difficult-to-treat pathogenic strains of bacteria. Here, we report that glycine, serine and threonine catabolic pathway is down-regulated in serum-resistant Escherichia coli, whereas exogenous glycine reverts the serum resistance and effectively potentiates serum to eliminate clinically-relevant bacterial pathogens in vitro and in vivo. We find that exogenous glycine increases the formation of membrane attack complex on bacterial membrane through two previously unrecognized regulations: 1) glycine negatively and positively regulates metabolic flux to purine biosynthesis and Krebs cycle, respectively. 2) α-Ketoglutarate inhibits adenosine triphosphate synthase, which in together promote the formation of cAMP/CRP regulon to increase the expression of complement-binding proteins HtrE, NfrA, and YhcD. The results could lead to effective strategies for managing the infection with serum-resistant bacteria, an especially valuable approach for treating individuals with weak acquired immunity but a normal complement system.
The human complement system acts either independently or together with the immune system to kill bacteria in the bloodstream and/or on mucosal surfaces1,2. However, a significant number of both Gram-negative and Gram-positive bacteria survive and proliferate in human blood that has a fully functional complement system. These pathogens are therefore referred to as serum-resistant bacteria, and they are associated with potentially serious systemic infections3,4,5. Serum resistance has been extensively investigated, but the mechanism remains poorly understood at the molecular level. Most studies attribute the serum resistance of Gram-negative bacteria to bacterial outer membrane proteins, lipopolysaccharide (LPS) and its antibodies, and capsular polysaccharide in inhibiting complement system activation6,7,8,9,10,11. Some outer membrane proteins bind complement-inhibiting factors, such as OmpA binding with C4b-binding protein, preventing the efficient formation of membrane attack complexes (MACs)12,13,14,15, while OmpC mutations increase bacterial survival in serum by inhibiting classical C1q and anti-OmpC antibody-dependent cell killing16. The serum resistome of E. coli EC958 comprised of 56 genes, where a major proportion of the genes encode membrane proteins or factors involving in LPS biosynthesis17. LPS is necessary for the survival of E. coli in the blood18. Immunoglobulin G to LPS confers serum resistance through shielding the bacteria from other antibodies that can induce complement-mediated killing10,19,20. The presence of capsular polysaccharide is correlated with serum resistance in bacteria11. Moreover, metabolism also plays roles in serum susceptibility, e.g., ferric uptake regulator (Fur) confers serum resistance21,22 and O2 consumption, adenine uptake, and glucose consumption increase are characteristic metabolic features when bacteria were exposed to serum23,24.
These findings provide a clue to the molecular basis of serum resistance in Gram-negative bacteria6,7,8,9,10,18,19,20,21,22. However, they do not provide insight into the metabolic state of serum-resistant bacteria, nor do they suggest promising strategies for managing infections with serum-resistant pathogens. To address this knowledge gap, our lab recently analyzed the metabolome of antibiotic-resistant bacteria. The results led us to propose that exogenous metabolite(s) might constitute a perturbation sufficient to restore antibiotic-sensitivity to multidrug-resistant bacteria25,26,27,28,29. We also showed there is a link between bacterial serum resistance with metabolism29,30. In the present study, we describe a framework based on functional metabolomics and metabolite perturbation, that might lead to a novel approach for controlling and/or preventing acute infection with serum-resistant and/or antibiotic-resistant pathogens.
Glycine catabolism is reduced in serum-survival E. coli
Comprehensive metabolomics provides information about the metabolic state of a cell or an organism, and how that state changes in different environmental contexts25,31. We first showed that non-pathogenic E. coli K12 was killed by serum but not by heat-inactivated (HI) serum and control without serum (Supplementary Fig. 1a, b). Then metabolome profiling was performed in these serum-treated bacteria as shown in (Supplementary Fig. 1c–e). In serum-survival E. coli K12 cells, 59 metabolites were present at significantly different concentrations than the control without serum (p < 0.01; Fig. 1a). Of the 59 metabolites, 44 were present at lower abundance in the presence of serum and 15 were present at higher abundance (Fig. 1b). A web-based metabolomics pathway analysis tool, MetPA, was used to evaluate the potential impact of differential metabolite abundance on metabolic pathways in the bacterial strain. This analysis identified 8 pathways differentially-expressed in E. coli K12 in the presence of serum, with the largest predicted differential effect involving glycine, serine and threonine metabolism, with secondary and tertiary impact on alanine, aspartate and glutamate metabolism and the Krebs cycle (TCA cycle), respectively (Fig. 1c, d). Hierarchical clustering analysis identified glycine, serine, and threonine as three of the critically down-regulated metabolites (Fig. 1e; Supplementary Fig. 2), substantiating glycine, serine and threonine metabolism is the most significantly altered pathway in serum-survival E. coli K12. The same analysis was performed for cells exposed to HI serum, displaying different signature and including increased glycine, serine, succinate, and fumarate (Supplementary Fig. 3).
qRT-PCR was used to demonstrate whether the reduced concentrations of glycine, serine, and threonine are linked to their catabolism or/and glycine transport. The expression of glyA, kbl, sdaA, sdaB, aceE, aceF, and gltA mediating glycine flux to the TCA cycle was decreased, whereas itaE and purD mediating glycine to threonine and glycinamide ribonucleotide, respectively, was elevated when exposed to serum (Fig. 1f). Meanwhile, serum repressed the transcription of glycine transporter gene, cycA (Fig. 1g). Thus, downregulation of glycine, serine and threonine catabolism to the TCA cycle is associated with active serum exposure, which is partly attributed to the decreased expression of glycine transporter. The further experiment demonstrated higher viability after re-exposure to serum (Fig. 1h).
Eight serum-resistant and eight serum-sensitive clinical isolates of E. coli were selected (Supplementary Fig. 4). Their metabolic profiles also show a lower abundance of glycine, serine and threonine in serum-resistant strains than in serum-sensitive strains (Fig. 1i). Thus, it is possible that low abundance of glycine, serine, and/or threonine could serve as a biomarker of serum-resistance.
Glycine, serine, and threonine restore serum sensitivity
We hypothesized that uptake of an exogenous metabolite might perturb the metabolome of a serum-resistant bacterium and thereby restore sensitivity to killing by serum. To demonstrate this, exogenous glycine, serine, or threonine was added to a final concentration of 100 mM and cell viability was measured. The viability in the presence of serum was reduced by 85.0-, 6.8-, or 8.9-fold, respectively (Fig. 2a). Exogenous glycine also significantly reduced viability of serum-susceptible strains E. coli K12, Y5, and serum-resistant strains Y10, Y17 in the presence of serum, while no killing was observed in the controls (Supplementary Fig. 5a) and the effect was dependent on glycine dose (Fig. 2b), serum concentration (Fig. 2c), and time (Fig. 2d, e). When serum was treated by anti-C3, EDTA, or EGTA, glycine failed to potentiate serum to kill bacteria (Fig. 2f; Supplementary Fig. 5b). Survival of E. coli Y17 was independent of the presence of serum during a 30-minute incubation, while under similar conditions, E. coli K12 showed approximately 65% survival. However, in the presence of serum and glycine, Y17 cells lost viability over time, with less than 1% survival after 6 h (Fig. 2d, e). Exogenous glycine did not potentiate ampicillin or gentamicin to kill bacteria under the same condition to exclude the possibility that exogenous glycine impairs bacterial cell wall integrity as reported in Gram-positive bacteria32 (Supplementary Fig. 5c), even if the integrity is key to bacterial survival in the presence of antibiotics33. In addition, sensitivity to serum was not restored by incubation in media containing various other amino acids and metabolites (Supplementary Fig. 5d). These data show that glycine, serine, or threonine specifically potentiates the ability of serum to kill strains of E. coli that are otherwise resistant to such killing.
Effect of exogenous glycine on metabolic state
As suggested earlier, it is possible that serum-resistant could be reverted to serum-susceptible based on the reprogramming metabolomes31. To test this, glycine was selected as a metabolome-reprogramming metabolite. Therefore, metabolomic profiles of 73 metabolites were compared in E. coli K12, as described above (Fig. 1a–e; Supplementary Fig. 1c–e), using bacteria grown in media or in media plus glycine, serum, or both (Supplementary Figs. 6 and 7a–c). As compared to the control group, metabolites belonging to glycine, serine and threonine metabolism and to TCA cycle were lower in serum-exposed bacterial cells but increased when exposed to serum plus glycine except for threonine, which was decreased (Fig. 3a; Supplementary Figs. 7d and 7e). However, when serum plus glycine group was compared to glycine group, the abundance of glycine and threonine was reduced, while the abundance of succinate, fumarate, malate, and oxaloacetate in the TCA cycle was increased, suggesting serum drives glycine flux to the TCA cycle (Supplementary Fig. 8). Therefore, these results suggest that serum plus glycine triggers a metabolic change leading to increased production of NADH via the TCA cycle (Fig. 3b).
NADH would increase the membrane potential, thus potentiating cell death in the presence of serum plus glycine. In fact, we observed that exogenous glycine or serum correlates with an increase in membrane potential in E. coli K12 and Y17, with a synergistic effect of serum plus glycine relative to controls (Fig. 3c). The ionophore carbonylcyanide m-chlorophenyl hydrazone (CCCP) abolished the synergistic increase in membrane potential (Fig. 3c) and bacterial percent survival (Fig. 3d) in E. coli K12 or Y17 cells exposed to serum plus glycine. These results suggest that increased membrane potential is crucial for the synergistic effect of serum and glycine to revert serum resistance. Interestingly, membrane potential is significantly higher in E. coli K12 than Y17, and a differential synergistic effect of serum and glycine on membrane potential is found for the two bacterial strains (Fig. 3c). The results are consistent with the finding that E. coli K12 is more susceptible to the bactericidal activity of serum than Y17, and thus verifies the synergistic effect of serum plus glycine on survival.
With regard to the importance of membrane potential in serum-dependent cell death, it may relate to the recruitment of positively-charged C3 to the negatively-charged cell surface34,35,36. When membrane potential is sufficiently high, protons and/or electrons are transported across the membrane, which drives the synthesis of ATP, a reaction catalyzed by ATP synthase. Consistent with this, a defect in ATP synthase increases susceptibility to serum, which likely correlates with increased membrane potential (Fig. 3e; Supplementary Fig. 9).
We further validate the finding that exogenous glycine stimulates the TCA cycle using NTFD (non-targeted tracer fate detection)37,38 (Fig. 3f; Supplementary Tables 1 and 2). Furthermore, qRT-PCR showed that the expression of genes mediating glycine catabolism was increased (Fig. 3g). These results indicate that exogenous glycine stimulates the TCA cycle.
Several other metabolites, including alanine, fructose, glucose, and glutamine stimulate the TCA cycle and increase membrane potential (Supplementary Fig. 10a)25,26,27; however, these metabolites did not decrease survival in the presence of serum (Supplementary Fig. 5d). This suggests that other factors may also be involved in addition to membrane potential.
Glycine flux to the TCA cycle is essential for promoting the serum killing
One possible explanation is that glycine promotes the expression of glycine-responsive genes, which in turn mediates the killing. To test this possibility, 12 E. coli K12 single deletion mutants of glycine metabolism were cultured in the presence or absence of glycine plus serum. Increased viability was observed in ∆glyA and ∆kbl (Fig. 4a; Supplementary Fig. 9). These two genes encoded essential proteins to generate serine and threonine, respectively. Thus, these two proteins are required downstream of glycine to promote serum-dependent bacterial cell death, but they do not explain the mechanism of cell death.
Therefore, metabolic flux involving serine and threonine was explored. Genes that promote conversion of threonine to glycine were required for the killing effect, but others were not (Fig. 4b; Supplementary Fig. 9). However, loss of genes, sdaA, and sadB, transferring serine to pyruvate, abrogated the killing (Fig. 4c; Supplementary Fig. 9). Thus, these three metabolites must flux to pyruvate to play a role, although interregulation among them existed, which is supported by the fact that the absence of itaE decreased the effect of glycine, and lower viability was detected in ∆glyA than ∆tdcB and ∆ilvA (Fig. 4a), suggesting it is required to flux threonine to glycine. Consistently, the viability of E. coli K12 cells was lower in the presence of serine than threonine at 6 h (Fig. 4d). When 50 mM serine or glycine was added, cell viability was equal at 6 h, but approximately four-fold fewer viable cells were detected than in the presence of the same concentration of threonine (Fig. 4e). We further demonstrated this through detection of glycine level in these mutants described in (Fig. 4a). The glycine concentrations were proportional to the percent survival of the mutants (Fig. 4f), indicating that glycine flux to the TCA cycle is essential. We also showed that loss of cycA increased cell viability (Fig. 4g). These results together support that glycine catabolism to the TCA cycle is critical to reverse serum resistance.
α-Ketoglutarate regulates ATP synthase
Following serine flux to the TCA cycle via pyruvate, we found gene-specific effects. In particular, the presence of genes in the pyruvate metabolism (aceE and aceF) and in the upper TCA cycle (gltA, acnA, acnB, icd, sucA, and sucB) induced rapid killing of bacteria by serum. In contrast, the presence of genes in the lower TCA cycle lacked this effect (Fig. 5a; Supplementary Fig. 9). These results were supported by the observation of increased serum-sensitivity in the presence of exogenous pyruvate, citrate, cis-aconitate, isocitrate or α-ketoglutarate, but not succinyl-CoA, succinate, fumarate, malate or oxaloacetate (Fig. 5b). To explain these results, we postulate that a defective step in the TCA cycle might lead to a defect in ATP synthesis. To explore this, ATP synthase activity was measured in the presence or absence of serine in E. coli K12, ∆aceE, ∆aceF, ∆gltA, ∆acnA, ∆acnB, and ∆icd; these results show a significant effect in these mutants, but not other single deletion mutants tested here. The final gene in the pathway, icd, converts isocitrate to α-Ketoglutarate (Fig. 5c). Consistent with this, the amount of ATP was decreased in these strains (Fig. 5d). Glycine and serine increased α-Ketoglutarate concentration (Fig. 5e) but not in ∆icd cells (Fig. 5f). These results indicate that α-Ketoglutarate might inhibit ATP synthase. To further demonstrate this, microscale thermophoretic analysis (MST) showed that α-Ketoglutarate interacts with AtpB, a subunit of ATP synthase (Fig. 5g). Exogenous glycine elevated intracellular α-Ketoglutarate in a dose-dependent manner, from 480 μM, a concentration reported previously39, to 750, 110, 1300, and 1450 μM by 10, 20, 50, and 100 mM glycine, respectively (Fig. 5h), leading to 270–970 μM (1.56–3.20 folds) of α-Ketoglutarate increase. The inhibition of α-Ketoglutarate to ATP synthase appeared in 750 μM and 325 μM α-Ketoglutarate in cell lysis and purified AtpB, respectively (Fig. 5i). Clearly, exogenous glycine promoted the abundance of α-Ketoglutarate but inhibited ATP synthase activity in a dose-dependent manner (Fig. 5j). Thus, this accumulation induced by exogenous glycine is enough to inhibit ATP synthase.
Exogenous glycine regulates purine metabolism
On the other hand, besides ATP synthase, ATP synthesis needs AMP and ADP. Therefore, a role for purine metabolism was explored, because mutations in purD, a gene involved in the de novo purine nucleotide biosynthesis, altered serum resistance (Fig. 4a), and reports have linked glycine metabolism with de novo purine synthesis40,41. A series of mutant strains of E. coli K12, each deleted for a gene in the purine biosynthetic pathway, were cultured in the presence and absence of glycine plus serum. The results show decreased viability of most strains with defects in purine biosynthesis (Fig. 6a), suggesting that purine biosynthesis influences serum-dependent cell death. We validated this by demonstrating that ∆purR cells, lacking a potent negative regulator of purine nucleotide metabolism, are more resistant to cell killing in the presence of serum and glycine than E. coli K12 (Fig. 6b; Supplementary Fig. 9). We supposed that this led to decreasing AMP level and thereby exogenous AMP and ATP might abolish the role of glycine. Consistent with the hypothesis, we detected a glycine-dependent decrease of AMP with time (Fig. 6c), and demonstrated that exogenous IMP, AMP, ADP, and ATP abolish glycine-dependent bacterial cell death (Fig. 6d). ATP analog had a similar effect as ATP (Fig. 6e). This finding suggests that ATP contributes to serum resistance.
PurR also negatively regulates glyA42. We also show that loss of purR led to increased expression of glyA/GlyA and kbl/Kbl using qRT-PCR and Western blot (Fig. 6f, g). MST and electrophoretic mobility shift assay (EMSA) confirmed the binding of PurR with glyA and kbl (Fig. 6h–j). However, the results on the negative regulation do not explain why exogenous glycine, serine, and threonine flux to the TCA cycle rather than purine metabolism via PurD. One possibility is that these three metabolites positively regulate GlyA or/and Kbl but not PurD through substrate activation, which is over the action negatively regulated by PurR. To explore this, qRT-PCR was used to detect expression to glyA, kbl, and purD in E. coli K12 and ∆purR cultured in medium with different concentrations of glycine, serine, or threonine. High dose of these metabolites favored increasing glyA and kbl expression in E. coli K12. In details, the expression of glyA and kbl were elevated by glycine and threonine, and the expression of glyA was increased by serine, whereas no significant change was detected in the expression of purD in response to three metabolites (Fig. 6k). Consistent with the negative regulation of PurR to glyA and kbl, lower glyA and kbl expression was detected in E. coli K12 than ∆purR (Fig. 6k). Western blot data also validated these findings, showing GlyA was increased in a dose-dependent manner, while the weak elevation of Kbl and no significant change of PurD were detected (Fig. 6l), and purD/PurD was not regulated by PurR (Fig. 6f, g). These results indicate that high dose of exogenous glycine leads to increased intracellular serine but lower purine biosynthesis; this may reflect the expression of elevated GlyA and unchanged PurD.
Mechanism of cell death in the presence of serum and glycine
These above results suggest that ATP plays a role in the serum-mediated killing. To demonstrate this, we detected a glycine-dependent decrease of ATP over time lapse (Fig. 7a) and found that exogenous ATP abolished glycine-dependent bacterial cell death (Fig. 7b). We also found that intracellular ATP was positively regulated by exogenous ATP, ATPγS, ADP, AMP, but negatively regulated by serum, glycine/serum (Supplementary Fig. 10b).
ATP is converted into cyclic-AMP (cAMP) by adenylate cyclase (coded by cyaA)43. cAMP and CRP (coded by crp) form cAMP-CRP complex, a global transcriptional regulator controlling a minimum of 378 target promoters in E. coli44. Consistent with this, synergistic action of glycine and serum led to a decreased abundance of cAMP, whereas single glycine or serum did not affect cAMP level (Fig. 7c); loss of cyaA and crp led to decreased viability (Fig. 7d; Supplementary Fig. 9). We also demonstrated that the abundance of CRP increases in an ATP- and AMP-dependent manner (Fig. 7e), whereas CRP expression is downregulated by glycine (Fig. 7f). These results suggest that exogenous glycine may decrease the abundance of the cAMP-CRP complex in cells exposed to serum, which is supported by the fact that absence of fis increases viability in the presence of glycine since fis negatively regulates crp (Fig. 7d; Supplementary Fig. 9)45.
CRP positively and negatively regulates flhC and csgD, two genes that regulate fimbrial and pilus usher proteins, respectively46,47. HtrE and YhcD are fimbrial usher proteins and NfrA is a bacteriophage N4 receptor with similar molecular mass as usher proteins. CRP regulates expression of outer membrane proteins in the manner of FlhC and CsgD47. Consistent with this, loss of flhC and csgD led to upregulation and downregulation of NfrA, HtrE, and YhcD, respectively (Fig. 7g) and the respective percent survival (Fig. 7d). Moreover, the expression of nfrA/NfrA, htrE/HtrE, and yhcD/YhcD was higher in a crp-deleted mutant than in wild type control (Fig. 7g, h). The results suggest that CRP regulates expression of NfrA, HtrE, and YhcD in the manner of FlhC and CsgD. In addition, loss of ompA and ompC led to lower percent survival in the presence of serum but not in the presence of serum and glycine (Supplementary Fig. 11).
Flow cytometry analysis confirmed significantly lowest binding of anti-C9 neoantigen of the terminal complement complex (TCC, MAC, the whole C5b-9 complex) to the triple mutants, ΔnfrAΔyhcDΔhtrE, ΔhtrEΔyhcDΔnfrA, and ΔyhcDΔhtrEΔnfrA, lower binding to the double mutants ΔnfrAΔyhcD, ΔhtrEΔyhcD, ΔyhcDΔhtrE, and low binding to the single mutants ΔnfrA, ΔhtrE, and ΔyhcD as compared to control (Fig. 7i). Corresponding percent survival was detected in these mutants (Fig. 7j). These are consistent with the hypothesis that HtrE, NfrA, and YhcD could be the outer membrane proteins that promote binding of complement components in the presence of exogenous glycine. Western blot analysis was then performed to measure relative expression of HtrE, NfrA, and YhcD in E. coli K12 grown in PBS, serum or in serum plus glycine. In the presence of both of serum and glycine, expression of HtrE, NfrA, and YhcD increased in a serum and glycine dose-dependent manner; in contrast, expression of HtrE, NfrA, and YhcD remained unchanged in the presence of media with added glycine, serum or saline (Fig. 7k, l). Similar results were obtained with E. coli Y17 and four pathogenic E. coli strains (Fig. 7m; Supplementary Fig. 12). These results indicate that glycine in the presence of serum stimulates expression of HtrE, NfrA, and YhcD.
To investigate whether the increased expression of HtrE, NfrA, and YhcD is contributing to the increased binding of components of the complement system, flow cytometry was used. Serum or serum plus glycine led to the deposition of C3b and C5b-9 complex on the outer membrane of E. coli K12 and Y17 (Fig. 7n). In particular, anti-C3b/iC3b and anti-C9 neoantigen recognized intact C3b, its degraded product, iC3b, and a neoepitope of TCC on the surface of serum or serum plus glycine-exposed E. coli K12 and Y17, respectively. Furthermore, TCC was detected at higher abundance by flow cytometry, when cell killing was more efficient, such that more C3b/TCC was present on the surface of E. coli K12 and Y17 exposed to serum plus glycine than on the surface of control cells exposed to saline, glycine, or serum. Consistent with this observation, more C3b/TCC was present on the surface of E. coli K12 than on Y17 after exposure to serum or serum plus glycine (Fig. 7n). MST assay demonstrated that HtrE, NfrA, and YhcD were bound to C3 (Fig. 7o). Far-Western blot and co-immunoprecipitation also showed that HtrE, NfrA, and YhcD interact with C3b, but not with IgG control (Supplementary Fig. 13). Therefore, glycine potentiates killing of serum-resistant bacteria, possibly by upregulating HtrE, NfrA, and YhcD, enabling the complement-dependent MAC-mediated cell death.
In addition, we investigated effect of O-antigen on the glycine-mediated killing, which is critical for LPS-mediated serum resistance in E. coli10,19,20. Among the 30 clinical E. coli strains, 27 strains were O-antigen positive (Supplementary Table 3). Waal works for LPS biosynthesis and is subjected to frequent mutations to generate serum resistance47. The mutations of waal were detected in randomly selected E. coli, P. aeruginosa, and K. pneumonia strains (Supplementary Fig. 14). E. coli serotype O157:H7, with adhesive fimbriae and a cell wall that consists of an outer membrane containing LPS, was sensitive to the glycine-triggering killing (Supplementary Fig. 15a) while exogenous glycine did not affect waal transcription (Supplementary Fig. 15b). When waal was deleted, the glycine-mediated killing was either unaffected or reduced by 4.81–24.1% (Supplementary Fig. 15c). These results indicate that LPS is not the key target in the glycine-triggering serum killing.
Loss of glyA confers serum resistance in serum-sensitive strains
To validate this possibility at a physiological level, we constructed glyA single deletion mutants in two serum-sensitive strains Y3 and Y21 as Y3ΔglyA and Y21ΔglyA, respectively. Y3 and Y21 were more susceptible to serum than E. coli K12, but Y3ΔglyA and Y21ΔglyA were highly resistant to serum at a similar level of a serum-resistant strain Y15. When exogenous glycine was supplemented, percent survival was reduced in all the five strains. However, higher killing efficacy was detected in Y21, Y3, K12 than Y15, Y21ΔglyA, Y3ΔglyA (Fig. 8a). Correspondingly, glyA deletion resulted in increased glycine level in both Y3ΔglyA and Y21ΔglyA (Fig. 8b). However, the increased glycine level did not inhibit ATPase activity and ATP production (Fig. 8c, d) and thereby increased the cAMP level (Fig. 8e). These results indicate that serum-susceptible bacteria exhibit serum resistance when no glycine fluxes to the TCA cycle.
CRP is a transcriptional factor modulated by cAMP. We further explored whether loss of glyA would impact CRP activity. qRT-PCR and Western blot showed that the deletion increased CRP expression at both mRNA and protein level (Fig. 8f, g). CRP positively and negatively regulates FlhC and CsgD, respectively, which downregulate the expression of nfrA, htrE, and yhcD46,48,49. Consistently, the deletion of glyA resulted in increased expression of flhC but decreased expression of csgD, nfrA, htrE, and yhcD (Fig. 8h). These results indicate that the elevated cAMP/CRP when glyA was depleted confers serum resistance through reducing NfrA, HtrE, and YhcD expression. Therefore, the metabolic flow of glycine to the TCA cycle is an essential step for sensitivity to serum-mediated killing.
The potential is effective to serum-resistant pathogens
To explore whether the glycine-mediated serum killing was applicable to a broad range of pathogens, more studies were performed on clinically-relevant multidrug-resistant E. coli, Klebsiella pneumonia, methicillin-resistant Staphylococcus aureus (MRSA), and Pseudomonas aeruginosa. These pathogenic bacteria were cultured in vitro in the absence or presence of serum or serum plus glycine. With the exception of a few E. coli strains and two strains of P. aeruginosa, all strains were serum-resistant. Cell death was 10- to 1430-fold greater in the presence of glycine and serum than in the presence of serum alone. The average (and range) of this effect was 143.97-fold (24.65–936.43) in E. coli, 67.08-fold (15.17–122.19) in K. pneumoniae, 49.28-fold (10.0–95.6) in MRSA, and 230.41-fold (11.76–1430.83) in P. aeruginosa (Fig. 9a). Notably, MRSA is a Gram-positive bacterium deficient of outer membrane targeted by the MAC complex. Our results showed that higher level of C3 and C5 on the surface when glycine was supplemented (Supplementary Fig. 16), which requires further study.
A mouse model was used to explore the efficacy of glycine for inhibiting pathogenic infections in vivo. Glycine was delivered by intravenous injection at a dose of 10, 15, or 20 mg glycine/mouse twice a day for 2 days. BALB/c mice were infected with E. coli strains K12, Y1, and Y17, K. pneumoniae strains 1924 and 2125, MRSA strains 1 and 5, and P. aeruginosa strains 1 and 23 via intraperitoneal injection. The results showed that bacterial load in the bloodstream, kidney, spleen, and liver of infected mice decreases in a glycine dose- and time-dependent manner (Fig. 9b, c, Supplementary Fig. 17). In the plasma, the concentration of glycine was increased dramatically and reached the plateau within 5 min after the first injection. Then the level was gradually decreased and was returned to the baseline after 1 h post-injection. A second injection was performed at 12 h after the first injection. A similar glycine change level was observed but with a bit of higher abundance (Supplementary Fig. 18). In contrast, the level of glycine was elevated in liver, kidney, and spleen in a dose-dependent manner at 48 h after the first injection (Fig. 9d). The glycine-enabled killing of bacteria by serum was detected in Rag1−/− null mice which do not bear mature T cells or B cells, supporting the glycine-mediated potential is independent of immunity endowed by the mature T cells or B cells (Fig. 9e). To demonstrate the role of glycine and complement in the potentiation, BALB/c mice were injected with K12, ΔcycA, or ΔnfrAΔyhcDΔhtrE, respectively. Bacterial load in the bloodstream, spleen, and liver was higher in ΔcycA-infected or ΔnfrAΔyhcDΔhtrE than K12-infected mice (Fig. 9f), thereby validating that glycine, and HtrE, NfrA, YhcD play roles.
We also showed that E. coli K12 cells remained inviable in vitro in the presence of serum plus glycine after five serum-killing/dilution-culture cycles, while showing weak susceptibility to serum in the absence of glycine (Supplementary Fig. 19).
Survival of E. coli K12 and Y17 decreased significantly in media with glycine plus serum from mouse and pig, as did survival of V. alginolyticus in media with glycine plus fish and shrimp plasma (Fig. 9g). This result suggests that the metabolic pathways and the biological basis of serum-dependent cell death in the presence of exogenous glycine are similar in mammals and some non-mammalian species.
We further explored whether glycine potentiates antibiotics to eliminate serum-susceptible or serum-resistant and multidrug-resistant bacterial pathogens. Percent survival of E. coli was lower in glycine with ampicillin or kanamycin than only glycine or the drugs (Supplementary Fig. 20). Mice were infected with pathogenic E. coli Y9 (serum-resistant and multidrug-resistant), E. coli Y23 (serum-susceptible and multidrug-resistant), P. aeruginosa 22 (serum-resistant and multidrug-resistant) or K. pneumonia 0367 (serum-resistant and multidrug-resistant). The synergistic use of glycine and ampicillin increases the survival rate of the infected mice. Specifically, mice infected with E. coli Y9 or E. coli Y23 had a 100% survival rate if both glycine and ampicillin were treated, and a 70% survival rate if the only glycine was treated, while the survival rate dropped to 0–10 % if treated with ampicillin alone or with saline. Similarly, mice infected with P. aeruginosa 22 or K. pneumonia 0367 had a survival rate of 70–80% when both of ampicillin and glycine were treated, and a 40–50% survival rate if only glycine was treated, while 10% survival was found if treated with ampicillin alone or with saline (Fig. 9h). These results indicate that glycine promotes ampicillin to kill serum-susceptible or serum-resistant and multidrug-resistant pathogens.
Serum resistance as bacterial pathogenesis was found more than a hundred years, but information regarding effective controls is not available. The present study highlights the way to eliminate serum-resistant bacteria through metabolome-reprogramming. The core findings of this study are: (1) downregulation of glycine, serine and threonine catabolism to the TCA cycle is a characteristic feature of serum-resistant bacteria; (2) glycine, serine and threonine catabolism, the TCA cycle, purine metabolism, ATP synthase, and ATP abundance, as well as PMF, play roles in serum-mediated cell death; (3) the cAMP/CRP complex modulates expression of HtrE, NfrA, and YhcD, which promotes complement binding, leading to MAC-mediated bacterial cell death; (4) the latter pathway plays a key role in serum-mediated cell death. These findings are summarized in (Fig. 10).
In the present study, two novel findings are interesting. First, a novel metabolic regulation pathway is disclosed. Glycine is consecutively transformed to α-Ketoglutarate and then α-Ketoglutarate interacts with ATP synthase. It is an interesting finding that bacterial α-Ketoglutarate inhibits ATP synthase, demonstrating a mechanism to maintain membrane potential that involves metabolite feedback. Second, we suggest that metabolite feedback may override the effects of global gene regulation. More specifically, although PurR negatively regulates glyA and kbl, a high concentration of glycine, serine, or/and threonine increased expression of glyA/GlyA and kbl/Kbl several-fold. In addition, the regulation of PurR to kbl and glyA to crp/CRP is found. And HtrE, NfrA, and YhcD are unknown complement-binding outer membrane proteins.
Animal protocols were approved by the University Committee for the Use and Care of Laboratory Animals. All work was conducted in strict accordance with the recommendations of the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health.
Bacterial strains and growth conditions
In this study, the parent strain E. coli K12 BW25113 (genotype, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), lambda−, rph-1, Δ(rhaD-rhaB)568, hsdR514) and its knockout strains were taken from the KEIO collection. A single colony of E. coli K12 BW25113 and its knockout strains were picked from the Luria-Bertani (LB) agar plate. Reliability of these mutants was validated by gene sequencing, which primers were listed in Supplementary Table 4. These bacteria were separately inoculated in 5 mL of LB medium for 16 h at 37 °C and spun at 200 rpm. Overnight cultures were diluted 1:100 in 50 mL fresh LB medium and grew to phase OD600 of 0.5 at 37 °C. Bacterial cells were collected by centrifugation at 8000 × g for 10 min at 4 °C and suspended in saline solution. Unless otherwise noted, 100 mM glycine was added and cultured at 200 rpm for 2 h in 37 °C in all experiments. Samples were pre-treated with the proton ionophore CCCP for 5 min in all experiments. The other bacteria, multidrug-resistant E. coli, P. aeruginosa, K. pneumonia, methicillin-resistant Staphylococcus aureus (MRSA), V. alginolyticus and V. parahaemolyticus were from the collections of our laboratory. These cells were insensitive to at least three classes of antibiotics including β-lactamases, aminoglycoside, quinolones or/and sulfonamides. Unless otherwise noted, the culture conditions were the same above. All bacterial strains used were listed in Supplementary Table 5.
Human serum was collected from 200 healthy donors. The collected sera were packaged and kept at −80 °C for future use. Package sera were not used repeatedly when they were taken from the refrigerator. The collected bacteria above were suspended with sterile saline and adjusted to OD600 of 1.0. Aliquots 3 mL of the bacteria were added in a 5 mL centrifugation tube and were centrifuged at 8,000 g for 10 min in 4 °C. The remnants of normal saline were removed by pipette. Then, 100 μL serum, C3-depleted serum (purified by anti-human C3 affinity chromatography), HI serum or serum treated with 10 mM EDTA or 20 mM EGTA drying in 5 mL tube and 150 μL saline solution containing desired metabolites were added as an experiment group and an equal concentration of saline solution was used for a control group. The mixtures were cultured at 200 rpm at 37 °C for 2 h. The residual cells were collected by centrifugation at 8,000 rpm for 5 min in 4 °C and were then re-suspended in 3 mL saline solution. The samples were serially diluted with sterile saline, and 10 μL aliquots were spot-plated onto LB agar plates. The plates were cultured at 37 °C and the colony-forming unit (CFU) were calculated the next day. Only those of dilutions yielding 20–200 colonies were enumerated to calculate CFU. The percent survival was determined by dividing CFU of the treatment sample by CFU of control. Experiments were repeated in at least three independent biological replicates.
GC-MS analysis was carried out with a variation on the two-stage technique25. Peak intensities were normalized to form a single matrix with Rt-m/z pairs for each file in the dataset. Statistical analysis was performed as follows50,51. The data were median centered and inter-quartile range (IQR) scaled per sample. Plotted Z-scores were calculated based on the mean and standard deviation of a reference set (control without serum, unless otherwise stated). Hierarchical clustering based on Pearson’s correlation was performed on the log-transformed normalized data after median centering per metabolite. The multivariate statistical analysis included independent component analysis (ICA) (http://metagenealyse.mpimp-golm.mpg.de/). ICA was used to discriminate sample patterns, to identify the metabolites associated with infection and to minimize the interindividual variation’s influence. Principal component analysis (PCA) was used to reduce the high dimension of the data set.
Measurement of NADH and cAMP concentration
NADH was measured with a commercial kit according to the manufacturer’s instruction (Cat. GMS60097.2, Genmed, Shanghai, China). Bacteria with 3 × 109 CFU were disrupted by sonication (20% output and 5 s interval time for a total of 3 min) in an ice-bath. For measurement of NADH, the samples were centrifuged at 12,000 × g for 10 min at 4 °C. Supernatants were collected and protein concentration was measured. A total of 0.3 μg of protein were used for NADH quantification. For measurement of cAMP, the samples were centrifuged at 12,000 × g for 5 min at 4 °C, and 100 µL supernatant of each sample was used for measurement according to the manufacturer’s instructions (Cat. K371, cAMP Direct Immunoassay kit (Colorimetric), BioVision, USA).
E. coli cells were adjusted to 1 × 106 cells/mL in saline, and with 10 µM DiOC2(3) for 30 min at 37 °C. One milliliter of cultures was analyzed on a FACSCalibur flow cytometer at 37 °C (Becton Dickinson, San Jose, CA, USA). Each sample was observed with forwarding versus side scatter and was gated before data acquisition, record count: 50,000 cells. The fluorescence of DIOC2(3)’s can be excited at 488 nm and emitted at 530 nm (green) or 610 nm (red). The green fluorescence is determined by size while the red fluorescence is determined by membrane potential. The ration of red to green indicated fluorescence intensity values of the gated populations. The computational formula of membrane potential = Log(103/2 × red fluorescence/green fluorescence)52.
Absolute ATP and α-Ketoglutarate levels and ATPase activity
Intracellular water content was measured as follows53. Bacterial cells were harvested in the OD600 of 1.0 by centrifugation for 10 min at 12,000 × g and washed twice by centrifugation with sterile saline. Followed by measurement of the wet and dry weight of the cells, the intracellular water content/g was calculated from the equation: 1–0.23 - D/F, where F is the wet weight and D is dry weight. The constant value is 0.23.
To quantify intracellular ATP, α-Ketoglutarate concentrations and F type ATPase activity, dry cells from 10 mL of 1.0 OD600 were dissolved in 100 μL PBS buffer (pH7.4) and disrupted by sonication (20% output and 5 s interval time for a total of 3 min) in an ice-bath. The solution was diluted 1:500 for measurement of ATP and α-Ketoglutarate concentrations and F-type ATPase activity, with BacTiter-Glo™ Microbial Cell Viability Assay (Cat. G8231, Promega, Madison, WI), α-Ketoglutarate Colorimetric/Fluorometric Assay Kit (Cat. K677, BioVision, USA) and F-type ATPase Activity Assay Kit (Cat. GMS50248.2, Genmed, Shanghai, China), respectively. The intracellular concentrations and enzyme activity unit were quantified according to the manufacturer’s manual and intracellular water content39,53.
Western blot, far-Western blot, and Co-IP
Western blot, far-Western blot, and Co-IP were performed as follows54,55,56. For Western blot, samples were dissolved in 15% SDS-PAGE. Rabbit polyclonal antibodies anti-HtrE, -NfrA, -YhcD, -GlyA, -Kbl, -PurD, -CRP (1:500 dilution) were used as primary antibodies, which came from our laboratory and validated by corresponding mutants. Horseradish peroxidase (HRP)-mouse anti-ribbit IgG (1:3000 dilution, Boson Biotech, Xiamen China) was used as the secondary antibody. For far-Western blot, recombinant proteins NfrA, HtrE YhcD on nitrocellulose membrane (NC) membranes were used as bait proteins to capture human serum complement C3b or IgG (1:100 dilution) and then anti-C3b or anti-IgG were used to recognize them, respectively. For Co-IP, beads coupled with C3b antibody (Hycult Biotech Inc., PA, USA) were incubated with a human serum to capture C3b as a bait protein. The bait protein was reacted with recombinant proteins NfrA, HtrE, and YhcD. Recovered pellets were isolated by SDS/PAGE and transferred to NC membranes and then recognized by corresponding antibodies anti- HtrE, -NfrA, or -YhcD. The membranes were developed using electro-chemi-luminescence ECL. Uncropped and unprocessed scans of all blots are attached in the Source Data file or as supplementary figures in the Supplementary Information.
Detection of O-antigens
Diagnostic sera for Entero-toxigenic Escherichia coli (Cat. TR301, TR302 & TR303) were commercially obtained from Ningbo Tianrun Bio-pharmaceutical Co. LTD, Ningbo, China. O-antigens were detected according to the manufacturer’s instruction. Briefly, two lines were drawn on a clean glass slide with a crayon to form two cells. In sterile conditions, one drop of diagnosed serum and sterile saline were added in the first cell as a control and the second cell as a test, respectively. 1 × 107 CFU E. coli were mixed in the second cell and then mixed in the first cell until these bacteria were mixed with saline or serum to evenly form an emulsion. The glass slides were gently shaken for 1–2 min and observed with the naked eye. A positive reaction was determined when a milky white agglutination appeared, while a negative reaction resulted from a still equal emulsion. The results could be confirmed under a low power microscope.
Generation of gene-deletion mutants
Knockout of Y3-glyA, Y21-glyA, Y1-waal, Y3-waal, Y7-waal, Y10-waal, Y12-waal, Y17-waal, Y20-waal, Y23-waal, Y25-waal, Y26-waal, Y28-waal, Y29-waal, ΔnfrAΔyhcD, ΔhtrEΔyhcD, ΔyhcDΔhtrE, ΔnfrAΔyhcDΔhtrE, ΔhtrEΔyhcDΔnfrA, and ΔyhcDΔhtrEΔnfrA was performed with one-step inactivation of chromosomal genes. For the construction of these mutants, primers used to clone the kanamycin cassette from pKD13 are listed in Supplementary Table 6. The PCR products were transformed into targeted strains bearing pSIM5 plasmid, which express lambda red recombinase (E. coli Y3 and Y21 for glyA-deletion, Y1, Y3, Y7, Y10, Y12, Y17, Y20, Y23, Y25, Y26, Y28, Y29 for waal-deletion, ΔnfrA, ΔhtrE and ΔyhcD for yhcD-, yhcD- and htrE-deletion, and ΔnfrAΔyhcD, ΔhtrEΔyhcD, ΔyhcDΔhtrE and for htrE-, nfrA- and nfrA-deletion, respectively). The transformants were selected on 50 μg/mL kanamycin. The deletion mutants were confirmed by PCR. Test primers are listed in Supplementary Table 5. Kanamycin cassette was removed by transforming pCP20 plasmid.
Detection of C3b/iC3b, C3, C5, and C9 neoantigen
Fluorescein isothiocyanate (FITC) conjugated monoclonal antibody to human complement factor C3b/iC3b (Cat. HM2286, clone 3E7) or a C9 neoantigen of the terminal complement complex (TCC) (Cat.HM2167F, clone aE11) were from Hycult Biotech Inc., PA, USA. Anti-C3 Rabbit pAb (Cat. 381678), anti-C5 (N-term) Rabbit pAb (Cat. 616722), and Fluorescein (FITC) affiniPure goat anti-rabbit IgG (Cat.511101) were from Zenbio, China. For detection of C3b/iC3b and C9 neoantigen, 106 CFU/mL diluted cells were mixed with 1 µg C3b/iC3b FITC-monoclonal antibody or 0.5 µg FITC-C9 neoantigen monoclonal antibody for 1 h at 37 °C. For detection of C3 and C5, 106 CFU/mL diluted cells were mixed with 1:20 C3 polyclonal antibody or 1:20 C5 (N-term) polyclonal antibody for 1 h at 37 °C. Unbound primary antibody was removed and then mixed with Fluorescein (FITC) affiniPure goat anti-rabbit IgG at 1:300 for 1 h at 37 °C. Aliquots 1 mL of culture was added into flow tubes and then analyzed on a FACSCalibur flow cytometer. Each sample was observed with forwarding versus side scatter and was gated before data acquisition, record count: 50,000 cells. Experiments were repeated in at least three independent biological replicates.
Isotope tracer experiment
Stable isotope-labeled compounds were detected by non-targeted detection37,38. E. coli K12 was cultured using an unlabeled glycine and a 1:1 mixture unlabeled glycine and [U-13C2] glycine. Three independent biological replicates were performed for each condition. GC-MS was performed using an Agilent 7890A GC equipped with an Agilent 5975C MS operating under electron impact (EI) ionization. The effectiveness of each tracer was gauged using the software downloaded from Internet57.
Protein-small molecular interaction
MST was carried out as follows58. AtpB, PurR, NfrA, HtrE or YhcD and the N-Hydroxysuccinimide (NHS) ester fluorescent dye solutions were mixed in 1:1 ratio and incubated for 30 min at room temperature in the dark. Serial dilutions of unlabeled α-Ketoglutarate, glyA_p and kbl_p and human complement C3 with PBS buffer were mixed with 20 μM of fluorescent-labeled AtpB (for AtpB-α-Ketoglutarate), PurR (for PurR-glyA_p and -kbl_p) and NfrA, HtrE or YhcD (for human complement C3) and then incubated for 5 min at room temperature. Sample 4 μL was loaded into MST-glass capillaries for MST-analysis in a NanoTemper Monolith NT.115. By plotting α-Ketoglutarate, glyA_p, kbl_p or human complement C3 concentration to ‰ changes of normalized fluorescence (△Fnorm [‰]), curve fitting was performed using GraphPad Prism software and Kd values were determined.
Electrophoretic mobility shift assay (EMSA)
Purified recombinant PurR and glyA promoter (212 bp) or kbl promoter (208 bp) were incubated in the binding buffer consisting of 250 mM Tris (pH 6.8), 150 mM NaCl, 5 mM EDTA, 10 mM DTT, 50% vol/vol glycerol. Electrophoresis was performed in an 8% polyacrylamide (29:1 acrylamide:bisacrylamide) gel, casted in 20 mM Tris–borate, 1 mM EDTA (pH 8.0), 1% (wt/vol) ammonium persulphate, 0.5% (vol/vol) TEMED and run in 20 mM Tris–borate, 1 mM EDTA (pH8.0) at 4 °C at 80 V and stained with a solution of 5 μg/mL ethidium bromide (EB).
Bacterial clearness in a mouse model
B6/JNju-Rag1em2Cd14/Nju mouse (six weeks), genotype:(Rag1)KO/KO were obtained from Nanjing Biomedical Research Institute of Nanjing University, Nanjing, China. BALB/c mice (8–10 weeks) were obtained from the Experimental Animal Center of Sun Yat-sen University, Guangzhou, China. These animals were acclimatized for one week and randomly divided into control and experimental groups (n = 6). The experimental groups were intraperitoneally injected with 10, 15 and 20 mg glycine/mouse, and control was injected with equal volume sterile saline, twice a day. After 2 days, 106 CFU E. coli K12, ΔcycA, ΔhtrE-nfrA-yhcD, P. aeruginosa, MRSA or K. pneumonia/mouse were injected. Blood was withdrawn at 24 h and 48 h (for E. coli dynamic observation), and spleen, kidney, and liver were collected at 24 h after the last injection to spot-plate and to observe CFU. Only those of dilutions yielding 20–200 colonies were enumerated to calculate CFU. During the experiment, no side effects were found in these animals. Animal protocols were approved by the University committee for the use and care of laboratory animals.
Glycine-potentiated killing by ampicillin in a mouse model
Six weeks BALB/c mice were obtained from the experimental animal center of Sun Yat-sen University, Guangzhou, China and were acclimatized for one week. These animals were intraperitoneally injected with of clinic E. coli Y9 (1.5 × 108 CFU), E. coli Y23 (2 × 108 CFU), P. aeruginosa P22 (5 × 105 CFU) or K. peneumoniae 0367 (2 × 107 CFU) per mouse by intravenous injection. The mice were randomly divided into four groups, ten per group and were intraperitoneally injected with saline (group 1), 800 mg kg−1 glycine (group 2), 320 mg kg−1 ampicillin (group 3) and glycine (800 mg kg−1) plus 320 mg kg−1 ampicillin (group 4). The injections were performed at 1 h, 36 h and 48 h post the infection, mouse survival was monitored for fifteen days.
UPLC-MS/MS to detect glycine in blood and internal organs
To determine glycine concentration, plasma was drawn from mice and mixed immediately with acetonitrile (1:3, v/v). The mixture was centrifuged twice at 12,000 × g for 10 min, and supernatants were collected. To determine glycine concentrations in liver, kidney, and spleen, these internal organs were removed from 10 untreated mice and 10 treated mice and weighted. These organs were homogenized in acetonitrile (1:2, w/v). Followed by centrifugation twice at 12,000 × g for 10 min, supernatants were collected. The supernatants from both plasma and organs were filtered through a 0.22 μM pore size hydrophilic PVDF membrane. Liquid chromatography was performed at 35 °C using a Waters ACQUITY UPLC system equipped with an Acquity BEH C18 column (50 mm × 2.1 mm i.d., 1.7 μm; Waters Corp.). Separation was using linear gradient elution with mobile phase A (acetonitrile) and B (0.2% formic acid in ultra-pure water) at a flow rate of 0.2 mL/min. The gradient elution was as follows: 0–3 min, 40% A and 60% B. The injection volume was 3 μL. Mass spectrometry experiments and optimization were carried out with QUATTRO PREMIER XE equipped with an electrospray ionization source operating in positive ionization mode (ESI+). The capillary voltage was set to 3000 V; the cone voltage was set to 20 V. The extractor voltage and RF Lens were set at 3 V and 0.5 V, respectively. The desolvation gas flow was set to 650 L/h at a temperature of 450 °C, the cone gas flow rate was set at 50 L/h and the source temperature was set at 120 °C.
The primers used for amplification are listed Supplementary Table 7. The deleted genes were amplified by PCR and cloned into the Hind III and BamH I sites of the pACYC184 Cm(+)vector or were constructed homologous recombination using pACYC184 Cm(+)vector. The recombinant plasmids were introduced into DH5α competent cells by electroporation under the condition of 2.5 kV voltage and 6 ms pulse time. The samples were incubated at 37 °C for 45 min and spread onto LB plates containing 50 μg/mL chloramphenicol at 37 °C. Recombinant plasmids were checked by digestion with restriction endonucleases and electroporated into E. coli deletion cells. The acquisition of the deletion mutants utilized a kanamycin-resistant gene to replace these genes by homologous recombination. Thus, these genes and their upstream and downstream of about 200 bp sequences were used for designation of primers. The resulting PCR products were approximately 1700 bp, containing kanamycin-resistant gene sequence (1303 bp), upstream (200 bp) and downstream (200 bp).
Total RNA samples were prepared from indicated tissues using TRIzol reagent (Invitrogen). Double-stranded cDNA was synthesized from total RNA using the SYBR Perfect real-time series kits (Takara). cDNA was analyzed by real-time RT-PCR using Roche’s LightCycler 480 real-time PCR system. Quantitative PCR was performed on each cDNA sample in triplicate. 16s rRNA was used as an internal control to normalize expression levels. Expression level was calculated using the comparative 2-ΔΔCt method. All samples were analyzed in three independent biological replicates with results expressed as the mean ± SEM59. The primers used for amplification are listed in Supplementary Table 8.
PCR and sequencing of waal
PCR was carried out in a routine procedure. Primers were listed in Supplementary Table 9. 50 μL reaction system contains 2 μl template DNA, 1 μL 10 μM upper or down primer, 2.0 μL 2.5 mM dNTP mix (TAKARA), 5 μL 10 × PCR polymerase buffer (TAKARA), 0.5 μL Taq polymerase (TAKARA) and appropriate amount of ddH2O. The PCR programs were applied as following: the denaturation temperature 94 °C for 3 min and 94 °C for 40 s; annealing temperature 56 °C for 30 s; extension at 72 °C for 90 s; and final extension 72 °C for 5 min; with 35 cycles of denaturation, annealing and extension phases. PCR products were checked with 0.8% agarose gel and were sequenced (BGI, Shenzhen, China).
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
All relevant data are available from the authors upon reasonable requests. The source data underlying all Main and Supplementary Figures and Tables are provided as a Source Data file.
Serruto, D., Rappuoli, R., Scarselli, M., Gros, P. & van Strijp, J. A. Molecular mechanisms of complement evasion: learning from staphylococci and meningococci. Nat. Rev. Microbiol. 8, 393–399 (2010).
Nakamura, S. et al. Molecular basis of increased serum resistance among pulmonary isolates of non-typeable Haemophilus influenzae. PLoS Pathog. 7, e1001247 (2011).
Schindler, M. K. et al. Yersinia enterocolitica YadA mediates complement evasion by recruitment and inactivation of C3 products. J. Immunol. 189, 4900–4908 (2012).
Koenigs, A. et al. CipA of Acinetobacter baumannii is a novel plasminogen binding and complement inhibitory protein. J. Infect. Dis. 213, 1388–1399 (2016).
Maruvada, R., Prasadarao, N. V. & Rubens, C. E. Acquisition of factor H by a novel surface protein on group B Streptococcus promotes complement degradation. FASEB J. 23, 3967–3977 (2009).
Clay, C. D., Soni, S., Gunn, J. S. & Schlesinger, L. S. Evasion of complement-mediated lysis and complement C3 deposition are regulated by Francisella tularensis lipopolysaccharide O antigen. J. Immunol. 181, 5568–5578 (2008).
Tan, L. A., Yang, A. C., Kishore, U. & Sim, R. B. Interactions of complement proteins C1q and factor H with lipid A and Escherichia coli: further evidence that factor H regulates the classical complement pathway. Protein Cell. 2, 320–332 (2011).
Smith, A. E. et al. PagP activation in the outer membrane triggers R3 core oligosaccharide truncation in the cytoplasm of Escherichia coli O157: H7. J. Biol. Chem. 283, 4332–4343 (2008).
Ho, D. K., Jarva, H. & Meri, S. Human complement factor H binds to outer membrane protein Rck of Salmonella. J. Immunol. 185, 1763–1769 (2010).
MacLennan, C. A. et al. Dysregulated humoral immunity to nontyphoidal Salmonella in HIV-infected African adults. Science 328, 508–512 (2010).
Diao, J. et al. Peptidoglycan association of murein lipoprotein is required for KpsD-dependent group 2 capsular polysaccharide expression and serum resistance in a uropathogenic Escherichia coli isolate. MBio. 8, e00603-17 (2017).
Tseng et al. NlpI facilitates deposition of C4bp on Escherichia coli by blocking classical complement-mediated killing, which results in high-level bacteremia. Infect. Immun. 80, 3669–3678 (2012).
Lindholm, M., Aung, K. M., Wai, S. N. & Oscarsson, J. Role of OmpA1 and OmpA2 in Aggregatibacter actinomycetemcomitans and Aggregatibacter aphrophilus serum resistance. J. Oral. Microbiol. 11, 1536192 (2019).
Prasadarao, N. V., Blom, A. M., Villoutreix, B. O. & Linsangan, L. C. A novel interaction of outer membrane protein A with C4b binding protein mediates serum resistance of Escherichia coli K1. J. Immunol. 169, 6352–6360 (2002).
Wooster, D. G., Maruvada, R., Blom, A. M. & Prasadarao, N. V. Logarithmic phase Escherichia coli K1 efficiently avoids serum killing by promoting C4bp-mediated C3b and C4b degradation. Immunology 117, 482–493 (2006).
Liu, Y. F. et al. Loss of outer membrane protein C in Escherichia coli contributes to both antibiotic resistance and escaping antibody-dependent bactericidal activity. Infect. Immun. 80, 1815–1822 (2012).
Phan, M. D. et al. The serum resistome of globally disseminated multidrug resistant uropathogenic Escherichia coli clone. PLoS Genet. 9, e1003834 (2013).
Crémet, L. et al. Innate immune evasion of Escherichia coli clinical strains from orthopedic implant infections. Eur. J. Clin. Microbiol Infect. Dis. 35, 993–999 (2016).
Coggon, C. F. et al. A novel method of serum resistance by Escherichia coli that causes urosepsis. MBio 9, e00920–18 (2018).
Wells, T. J. et al. Increased severity of respiratory infections associated with elevated anti-LPS IgG2 which inhibits serum bactericidal killing. J. Exp. Med. 211, 1893–1904 (2014).
Huja, S. et al. Fur is the master regulator of the extraintestinal pathogenic Escherichia coli response to serum. Mbio 5, e01460–14 (2014).
Hu, Y. H. & Sun, L. The global regulatory effect of Edwardsiella tarda Fur on iron acquisition, stress resistance, and host infection: a proteomics-based interpretation. J. Proteom. 140, 100–110 (2016).
Cohen, M. S. & Cooney, M. H. A bacterial respiratory burst: stimulation of the metabolism of Neisseria gonorrhoeae by human serum. J. Infect. Dis. 150, 49–56 (1984).
Dankert, J. R. Complement-mediated inhibition of function in complement-resistant Escherichia coli. J. Immunol. 142, 1591–1595 (1989).
Peng, B. et al. Exogenous alanine or/and glucose plus kanamycin kills antibiotic-resistant bacteria. Cell Metab. 21, 249–261 (2015).
Su, Y. B., Peng, B., Han, Y., Li, H. & Peng, X. X. Fructose restores susceptibility of multidrug-resistant Edwardsiella tarda to kanamycin. J. Proteome Res. 14, 1612–1620 (2015).
Su, Y. B. et al. The pyruvate cycle increases aminoglycosides efficacy and provides respiratory energy in bacteria. Proc. Natl Acad. Sci. USA 115, E1578–E1587 (2018).
Yang, J. et al. NaCl promotes antibiotic resistance by reducing redox states in Vibrio alginolyticus. Environ. Microbiol. 20, 4022–4036 (2018).
Wang, Z. et al. GC–MS-based metabolome and metabolite regulation in serum-resistant Streptococcus agalactiae. J. Proteome Res. 15, 2246–2253 (2016).
Cheng, Z. X. et al. Edwardsiella tarda tunes tricarboxylic acid cycle to evade complement-mediated killing. Front Immunol. 8, 1706 (2017).
Peng, B., Li, H. & Peng, X. X. Functional metabolomics: from biomarker discovery to metabolome reprogramming. Protein Cell 6, 628–637 (2015).
Hammes, W., Schleifer, K. H. & Kandler, O. Mode of action of glycine on the biosynthesis of peptidoglycan. J. Bacteriol. 116, 1029–1053 (1973).
Wolf, D. & Mascher, T. The applied side of antimicrobial peptide-inducible promoters from Firmicutes bacteria: expression systems and whole-cell biosensors. Appl Microbiol Biotechnol. 100, 4817–4829 (2016).
Murthy, K. H. et al. Crystal structure of a complement control protein that regulates both pathways of complement activation and binds heparan sulfate proteoglycans. Cell 104, 301–311 (2001).
Chonn, A., Cullis, P. R. & Devine, D. V. The role of surface charge in the activation of the classical and alternative pathways of complement by liposomes. J. Immunol. 146, 4234–4241 (1991).
Richards, R. L., Gewurz, H., Osmand, A. P. & Alving, C. R. Interactions of C-reactive protein and complement with liposomes. Proc. Natl Acad. Sci. USA 74, 5672–5676 (1977).
Hiller, K., Metallo, C. & Stephanopoulos, G. Elucidation of cellular metabolism via metabolomics and stable-isotope assisted metabolomics. Curr. Pharm. Biotechnol. 12, 1075–1086 (2011).
Walther, J. L., Metallo, C. M., Zhang, J. & Stephanopoulos, G. Optimization of 13C isotopic tracers for metabolic flux analysis in mammalian cells. Metab. Eng. 14, 162–171 (2012).
Bennett, B. D. et al. Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli. Nat. Chem. Biol. 5, 593–599 (2009).
Wilson, R. L., Stauffer, L. T. & Stauffer, G. V. Roles of the GcvA and PurR proteins in negative regulation of the Escherichia coli glycine cleavage enzyme system. J. Bacteriol. 175, 5129–5134 (1993).
Pacold, M. E. et al. A PHGDH inhibitor reveals coordination of serine synthesis and one-carbon unit fate. Nat. Chem. Biol. 12, 452–458 (2016).
Steiert, J. G., Rolfes, R. J., Zalkin, H. & Stauffer, G. V. Regulation of the Escherichia coli glyA gene by the purR gene product. J. Bacteriol. 172, 3799–3803 (1990).
Gstrein-Reider, E. & Schweiger, M. Regulation of adenylate cyclase in E. coli. EMBO J. 1, 333–337 (1982).
Pérez-Morales, D. & Bustamante, V. H. The global regulatory system Csr senses glucose through the phosphoenolpyruvate: carbohydrate phosphotransferase system. Mol. Microbiol. 99, 623–666 (2016).
Berthoumieux, S. et al. Shared control of gene expression in bacteria by transcription factors and global physiology of the cell. Mol. Syst. Biol. 9, 634 (2013).
Hufnagel, D. A. et al. The catabolite repressor protein-cyclic AMP complex regulates csgD and biofilm formation in uropathogenic Escherichia coli. J. Bacteriol. 198, 3329–3334 (2016).
Han, Y. et al. The waaL gene is involved in lipopolysaccharide synthesis and plays a role on the bacterial pathogenesis of avian pathogenic Escherichia coli. Vet. Microbiol. 172, 486–491 (2014).
Lemke, J. J., Durfee, T. & Gourse, R. L. DksA and ppGpp directly regulate transcription of the Escherichia coli flagellar cascade. Mol. Microbiol. 74, 1368–1379 (2009).
Jørgensen, M. G. et al. Small regulatory RNAs control the multi-cellular adhesive lifestyle of Escherichia coli. Mol. Microbiol. 84, 36–50 (2012).
Liu, S. R., Peng, X. X. & Li, H. Metabolic mechanism of ceftazidime resistance in Vibrio alginolyticus. Infect. Drug Resist. 12, 417–429 (2019).
Sreekumar, A. et al. Metabolomic profiles delineate potential role for sarcosine in prostate cancer progression. Nature 457, 910e4 (2009).
Cheng, Z. X. et al. The depressing central carbon and energy metabolisms mediate levofloxacin resistance in Vibrio alginolyticus. J. Proteom. 181, 83–91 (2018).
Unemoto, T. & Hayashi, M. Regulation of internal solute concentrations of marine Vibrio alginolyticus in response to external NaCl concentration. Can. J. Microbiol. 25, 922–926 (1979).
Wang, Y. et al. Comparative extracellular proteomics of Aeromonas hydrophila reveals iron-regulated secreted proteins as potential vaccine candidates. Front. Immunol. 10, 256 (2019).
Yao, Z. et al. Integrated succinylome and metabolomics reveals lysine succinylated LuxS plays a crucial role in quorum sensing and metabolism of Aeromonas hydrophila. Mol. Cell Proteom. 18, 200–215 (2019).
Li, H. et al. Alterations of protein complexes and pathways in genetic information flow and response to stimulus contribute to Escherichia coli resistance to balofloxacin. Mol. Biosyst. 8, 2303–2311 (2012).
Hiller, K. et al. NTFD–a stand-alone application for the non-targeted detection of stable isotope-labeled compounds in GC/MS data. Bioinformatics 29, 1226–1228 (2013).
Wienken, C. J., Baaske, P., Rothbauer, U., Braun, D. & Duhr, S. Protein-binding assays in biological liquids using microscale thermophoresis. Nat. Commun. 1, 100 (2010).
Jiang, M. et al. Phenylalanine enhances innate immune response to clear ceftazidime-resistant Vibrio alginolyticus in Danio rerio. Fish. Shellfish Immunol. 84, 912–919 (2019).
This work was sponsored by grants from the National Key Research and Development Program of China (2018YFD0900501/4), NSFC projects (U1701235, 31822058, 31770045, 41276145), and The Blue Life Breakthrough Program of LMBB for Pilot National Laboratory, Marine Science and Technology (Qingdao), China (MS2018NO06).
The authors declare no competing interests.
Peer review information: Nature Communications thanks Jim Cassat and Timothy Wells for their contribution to the peer review of this work. Peer reviewer reports are available.
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Cheng, Zx., Guo, C., Chen, Zg. et al. Glycine, serine and threonine metabolism confounds efficacy of complement-mediated killing. Nat Commun 10, 3325 (2019). https://doi.org/10.1038/s41467-019-11129-5
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