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A synthesis strategy for tetracyclic terpenoids leads to agonists of ERβ

Abstract

Natural product and natural product-like molecules continue to be important for the development of pharmaceutical agents, as molecules in this class play a vital role in the pipeline for new therapeutics. Among these, tetracyclic terpenoids are privileged, with >100 being FDA-approved drugs. Despite this significant pharmaceutical success, there remain considerable limitations to broad medicinal exploitation of the class due to lingering scientific challenges associated with compound availability. Here, we report a concise asymmetric route to forging natural and unnatural (enantiomeric) C19 and C20 tetracyclic terpenoid skeletons suitable to drive medicinal exploration. While efforts have been focused on establishing the chemical science, early investigations reveal that the emerging chemical technology can deliver compositions of matter that are potent and selective agonists of the estrogen receptor beta, and that are selectively cytotoxic in two different glioblastoma cell lines (U251 and U87).

Introduction

The means by which organic molecules can be synthesized has a profound impact on medicinal chemistry and drug discovery. Simply stated, without efficient and flexible processes capable of preparing compositions of matter with drug-like properties, medicinal chemistry programs cannot easily materialize. While it is well appreciated that natural products are a rich source of leads for drug discovery, it is also understood that their molecular architectures are typically quite challenging to address with de novo synthesis. In fact, organic chemistry to enable broad investigation of structure–activity relationships associated with medicinally relevant natural products is often not available. This reality is one of the many factors that continue to fuel developments in the field of chemical synthesis, where attention is directed at providing de novo means of producing natural product and natural product-inspired agents with increased efficiency and molecular flexibility.

Of the vast classes of natural products known to possess important biological and medicinal properties, perhaps none is more proven as a source of therapeutics than tetracyclic terpenoids. This large class of molecules, including steroids and cardiac glycosides, has had a transformative impact on our society (Fig. 1a; C-18 and C-19 cores)1,2,3,4,5,6. Today, scores of such agents are FDA-approved drugs used to treat a wide range of conditions, and this success supports the conclusion that the basic molecular skeleton associated with the class is a validated platform for drug discovery and development.

Fig. 1
figure1

Tetracyclic terpenoid skeletons and a means of synthesis from epichlorohydrin. a The general skeleton of tetracyclic terpenoids (C18 through C20). b A unified enantiospecific route to a variety of tetracyclic terpenoid motifs. c Application to the synthesis of a euphane-based tetracyclic skeleton and the synthesis and discovery of a potent and selective agonist of the estrogen receptor beta (ERβ)

Given this standing in medicinal chemistry, it is perhaps surprising that tetracyclic terpenoids persist as significant challenges for de novo synthesis. While numerous achievements have described synthetic forging of members of the broad class7,8,9,10,11,12,13, and the scientific advances central to such triumphs in natural product total synthesis have had a profound impact on the evolution of organic chemistry as a discipline, it remains the case that investigation of structure–activity relationships and production of active pharmaceutical ingredients (APIs) based on tetracyclic terpenoids typically proceed by derivatization of readily available natural products (a synthesis activity otherwise known as semisynthesis)12.

This reality greatly constrains efforts aiming to investigate the medicinal potential of diverse compositions of matter inspired by this natural product class, and has likely contributed to the fact that 100% of FDA-approved drugs in the area have the natural absolute stereochemistry (the mirror image isomer observed in nature). Accepting that the drug-like physical properties of a molecule are not strictly dependent on absolute stereochemistry, it appears as though a vast region of pharmaceutically relevant chemical space has remained underexplored for decades14,15,16,17.

In nature, tetracyclic terpenoids possess a wide variety of molecular skeletons that differ dramatically from the common enantiodefined C18 and C19 core skeletons of well-studied steroid hormones (Fig. 1a). In fact, many subclasses of natural products in this area that have not been thoroughly investigated in biology and medicine possess additional quaternary centers at C8, C9, and C14, and display unique relative stereochemistry with respect to each quaternary center.

Here, as summarized in Fig. 1b, c, a unified asymmetric entry to tetracyclic systems related to the large and diverse class of tetracyclic terpenoids is described. This chemical technology is shown to be capable of accessing the euphane tetracyclic system, while also delivering potent and selective agonists of the estrogen receptor beta (ERβ).

Results

Design of the synthetic route

Studies began with recognition that the challenges associated with enantioselective synthesis of steroid hormones are distinct from those associated with highly oxygenated and densely functionalized tetracyclic terpenoids that possess widely varying substitution and stereochemistries (Fig. 2a, b). Hoping to establish a unified theme capable of addressing the key molecular challenges associated with a variety of tetracyclic terpenoid skeletons (i.e., addressing the location and relative stereochemistry of characteristic quaternary carbon centers), the synthetic strategy depicted in Fig. 2c was conceived. Taking a departure from previous studies aimed at the enantiospecific synthesis of estranes18, it was thought that metallacycle-mediated annulative cross-coupling19 between a readily available enyne (2) and trimethylsilylpropyne 3 would deliver hydrindane intermediate 4, possessing the C13 quaternary center common to this large natural product class. Notably, and consistent with previous studies, it was anticipated that 4 would be formed with high levels of regioselectivity, positioning the trimethylsilyl (TMS) group at C11 of the carbocyclic nucleus.

Fig. 2
figure2

Design of a synthesis strategy to access varied tetracyclic terpenoid skeletons. a Examples of steroid hormones including an estrane, an androstane, and a pregnane. b Complex tetracyclic terpenoids that present distinct challenges for chemical synthesis in comparison with relatively simple steroid hormones. c Design of a concise approach to the enantiospecific assembly of C19 tetracyclic terpenoid core skeletons 5 and 6

With hydrindane 4 in hand, it was thought possible to introduce a C9 quaternary center simply by engaging the vinylsilane moiety in initial acid-mediated protodesilylation, followed by a second regioselective protonation of the diene to deliver a transient fully substituted allylic cation intermediate (not depicted). Intramolecular regio- and stereoselective Friedel–Crafts alkylation was then envisioned as a means to establish the fused tetracyclic skeleton 5—a molecular composition that has structural features reminiscent of numerous natural products (i.e., cayaponoside C2, Fig. 2b). Rather than serving as a terminal intermediate of relevance for a relatively small collection of terpenoid targets harboring a C9 quaternary center, it was thought that this intermediate may also serve as a platform to access numerous other terpenoid skeletons. Specifically, a sequence consisting of A-ring methyl ether cleavage followed by a unique oxidative rearrangement marked by a 1,2-alkyl shift from C9 to C10 was imagined to pave a path from 5 to the tetracyclic system 6. Notably, this tetraene-containing product contains the C10/C13 quaternary centers seen in numerous steroid hormones, cardiac glycosides, limonoids, euphanes, and lanostanes (among others).

While oxidative alkyl shift reactions have been described as a means to convert phenols to dienones possessing a quaternary center20,21,22, these reactions are typically conducted with substrates that enable C–C bond formation through a semi-pinacol-like process (substrates containing a benzylic tertiary alcohol), where termination of the cationic rearrangement is coupled to the formation of a ketone. The proposed process for conversion of 5 to 6 in Fig. 2c calls for migration of a methyl group from a quaternary center, generation of a tertiary allylic cation, and selective loss of a proton from C15 of the D-ring. Besides not having strong precedent in support of this proposed molecular transformation, at the outset of these studies methyl migration in oxidative dearomatization chemistry was appreciated as being notoriously problematic21,22.

Asymmetric synthesis of tetracyclic terpenoid skeletons

As illustrated in Fig. 3a, epichlorohydrin (ent-1) was converted to epoxide 7 by an established two-step procedure23, and subsequent BF3•OEt2-promoted addition of an acetylide derived from 8 provided the stereodefined substrate 9 in 85% yield. Regio- and stereoselective annulative cross-coupling with TMS-propyne 3 then delivered hydrindane 10 in 70% yield as a single regio- and stereoisomer19. Next, conditions were explored to accomplish the desired tandem protodesilylation and cyclization reaction to form the C9–C10 bond and establish the C9 quaternary center. As illustrated in the highlighted table, treatment of 10 with BF3•OEt2 in nitromethane at 0 °C, converted the hydrindane intermediate to the fully functionalized tetracycles 11 and 12. Here, cyclization appeared to occur only at C9 of the intermediate allylic cation (rather than at C14), with moderate selectivity for production of the anti-isomer 11 (dr = 6:1; the major product possesses a quaternary center at C9 that projects the methyl group on the opposite face of the tetracycle as the preixisting group at C13).

Fig. 3
figure3

Establishment of an asymmetric synthesis of tetracyclic terpenoid skeletons from epichlorohydrin. a Establishing a synthetic route from epichlorohydrin to steroidal tetracycles bearing a quaternary center at C9. b A proposed orientation of (S)-Binol•SnCl4 in the site- and stereoselective cyclization reaction. c Demonstration that the C16 (D-ring) alcohol is not important for stereocontrol in the acid-mediated cyclization reaction. d Development of an oxidative shift process for the generation of a steroidal tetracycle. e Application of this strategy to access an isomeric skeleton of relevance for euphane and tirucallane natural products. f Combining an oxidative alkyl shift with an alkoxide-directed Simmons–Smith cyclopropanation in a sequence that forges additional quaternary centers at C4, C10, and C14

In an effort to both enhance diastereoselection for this cyclization and to alter the inherent facial preference for the process, chiral Brønstead acids derived from complexation of Binol to a Lewis acid were explored. This strategy was introduced by Professor Yamamoto and further studied by Professor Corey as a means to achieve enantioselective polyene cyclization reactions24,25, and has to our knowledge not been investigated for related double-asymmetric Friedel–Crafts type cyclization chemistry. To establish a baseline for understanding simple diastereoselection in this double-asymmetric annulation process, an achiral system based on the combination of o,o’-dihydroxybiphenyl•SnCl4 in CH2Cl2 at −78 °C was first studied. Notably, the selectivity for formation of the anti-isomer in this cyclization reaction was increased to 9:1. Next, use of enantiodefined Binols in complex with SnCl4 or SbCl5 led to the discovery of a clear double asymmetric relationship. In the mismatched case, use of (R)-Binol•SbCl5 delivered the cyclized products as an 8:1 mixture of anti-(11) and syn-(12) stereoisomers,  and use of (R)-Binol•SnCl4 resulted in production of the syn-isomer in approximately an equal ratio with the anti-isomer (52% combined yield).

In contrast, the matched case proved to be highly stereoselective. Treatment of 10 with (S)-Binol•SnCl4 delivered the anti-isomer 11 in 50% yield with ≥ 20:1 stereoselectivity. Building on the empirical model first offered by Yamamoto24 for enantioselective Brønstead acid-mediated polyene cyclization, it is proposed that the relevant Binol•SnCl4 complex promotes cyclization of substrates like 10 through an arrangement similar to that depicted in Fig. 3b. While the factors that contribute to controlling selectivity in this reaction will be the subject of ongoing investigations, at this early stage of development it has been established that the D-ring free hydroxy group (at C16) is not an essential feature for stereoselection in this unique double-asymmetric Brønstead acid-mediated ring-closing process. As illustrated in Fig. 3c, the protected substrate 13 (C16 methyl ether) can be smoothly converted to a stereodefined tetracycle (14) in 67% yield, and with similarly high levels of stereocontrol (ds ≥ 20:1).

Moving forward, a concise sequence of steps was realized to transform each of the products of double-asymmetric Friedel–Crafts cyclization to tetracyclic C19 skeletons typical of steroid hormones. As depicted in Fig. 3d, reductive demethylation of the enantiodefined syn-isomer ent-12 (prepared from ent-10) with diisobutylaluminum hydride delivered the corresponding phenol in high yield26. The designed oxidative rearrangement was then found to proceed smoothly by treatment with (diacetoxyiodo)benzene (also referred to as phenyliodine(III) diacetate, or PIDA)20,21,22. Presumably acting by way of intermediate 15, stereospecific 1,2-methyl shift from C9 to C10 is thought to deliver a highly stabilized tertiary allylic carbocation 16 that is terminated by selective loss of a proton from C15, leading to production of the stereodefined dienone 17—a tetracyclic product that houses the quaternary centers at C10 and C13 that are seen in scores of tetracyclic terpenoid targets known to possess an array of medicinally relevant properties. Notably, the basic strategy to access 17 is equally effective for preparing the stereoisomeric tetracycle 20 (Fig. 3e); a compound that possesses the stereochemical features at C10 and C13 that are common in limonoids and euphane and tirucallane natural products.

As illustrated in Fig. 3f, this reactivity proved effective for establishing molecular features that are prominent in the family of tetracyclic triterpenoids exemplified in Fig. 2a (i.e., euphol and kansenone). After coupling epoxide 7 to alkyne 21, the resulting enyne (22) was transformed to hydrindane 23 by alkoxide-directed metallacycle-mediated annulative cross-coupling. Next, the double-asymmetric Brønstead acid-promoted cyclization process proved highly effective for coversion of 23 to the tetracycle 24 (78% yield; ds ≥ 25:1), and subsequent demethylation and oxidative rearrangement delivered the tetraene 25 as a single isomer. Installation of the additional quaternary center at C14 was then accomplished by capitalizing on the stereochemistry at C16 that was previously employed to both enable and control the regio- and stereochemical course of the metallacycle-mediated annulation reaction. While a number of strategies may be considered to achieve such a functionalization process (i.e., [2,3] or [3,3]-sigmatropic rearrangement chemistry), hydroxyl-directed Simmons–Smith cyclopropanation27 was selected for study here. Gratifyingly, this approach proved highly effective at establishing the trans-fused hydrindane system featuring adjacent quaternary centers at C13 and C14. Subsequent treatment with Co2(CO)8 and H2O resulted in site-selective A-ring enone reduction28, and furnished the stereodefined intermediate 26 in 69% yield. Finally, a one-pot Birch reduction and alkylation delivered the fully functionalized and differentiated product 27 that possesses the typical quaternary carbons at C4, C10, C13, and C14 observed in euphane natural products in 52% yield as a single stereoisomer.

While further synthetic manipulation of intermediates like 27 was not of interest in this study, the chemical transformations depicted in Fig. 3 realize a common synthetic pathway for enantiospecific access to tetracyclic terpenoid skeletons possessing a variety of stereochemical relationships, as well as spatially distinct patterns of fully substituted sp3 carbons (quaternary centers). Furthermore, the highly unsaturated nature of many of the key intermediates provide opportunities to exploit types of functionalization chemistry not typically encountered in other synthetic approaches to such tetracycle formation (i.e., intermediates accessible through biomimetic cation–olefin cyclization). As such, it is anticipate that this suite of chemistry has the potential to offer convenient access to synthetic agents in the broader class that are not easily prepared through other strategies for de novo synthesis, and that are even wholly incompatible with efforts that embrace semisynthesis (functionalization of readily available natural tetracyclic terpenoid starting materials).

Discovery of estrogen receptor beta (ERβ) agonists

With recognition that the chemical pathway established proceeds by way of structurally unique partially aromatic tetracycles in the estrane series (i.e., 11 and 12; Fig. 3a), attention was directed toward investigating the potential value of these species outside of their future role as synthetic intermediates in natural product and function-oriented synthesis campaigns. In fact, relatively few natural tetracyclic terpenoids have the core structure seen in the intermediates accessed here (after double-asymmetric Friedel–Crafts cyclization), and none have the combination of substitution and unsaturation seen in 11 and 12. That said, it was appreciated that partially aromatic synthetic steroidal systems have been described as modulators of estrogen receptors, ERα and ERβ (vide infra).

In particular, the estrogen receptor beta (ERβ) is a nuclear hormone receptor that was discovered in 1996 and has been recognized as a potentially important pharmaceutical target29,30. This nuclear hormone receptor is distinct from ERα; it is not expressed in the pituitary and has little role in the proliferation of the mammary gland or endometrium30. Agonism of ERβ is of great current pharmaceutical interest due to a variety of observations that include decreasing survival of p53-defective cancer cells by imparing G2/M checkpoint signaling31, playing a role as a tumor suppressor of epithelial ovarian cancer32 and of gliomas33, reducing invasiveness of triple-negative breast cancer cells34, inducing cell death in acute myeloid leukemia35, and decreasing proliferation of ovarian cancer cells36. Notably, ERβ has also been considered as a target of potential value for neuroprotection37, diabetes38, inflammation and pain39,40,41,42.

Being aware that 9α-substituted estratrienes have been described as selectively active estrogens43, we sought to investigate the ER-modulating properties associated with some molecules now readily available from the chemical advances described herein. Notably, unlike most explorations into the bioactivity of steroidal systems, the synthetic chemistry established here provides straightforward access to both enantiomeric series, and delivers unique compositions of matter whose additional unsaturation at C8 offers unique physical properties (i.e., altered solubility and rigidity) along with subtle perturbation of three dimensional molecular structure in comparison to related saturated variants.

As illustrated in Fig. 4a, the enantiomeric tetracycles 28 and ent-28, each prepared in only six steps from epichlorohydrin (as previously presented in Fig. 3a), were found to be potent and selective agonists of ERβ in an in vitro, bioluminescence cellular-reporter assay. Here, 17β-estradiol was used as a control, as it is appreciated to be a potent agonist of both ERα and ERβ. The enantiomer of interest for the de novo synthesis of euphane- and tirucallane-based natural products (28) was found to induce partial agonism of ERβ with an EC50 of 1.9 nM and clear selectivity for ERβ vs. ERα in comparison with 17β-estradiol. Speaking to the great impact that absolute stereochemistry can have on the biological and/or medicinal properties of organic molecules, the enantiomeric tetracycle (ent-28) proved to be markedly more potent and selective than its antipode — it was found to be a highly potent full agonist of ERβ with an EC50 value of 0.05 nM, and a superior selectivity profile over ERα. To our knowledge, these novel compositions of matter have unprecedented properties as selective agonists of ERβ33,36,37,41,42,44,45,46,47,48,49, while offering a validated and pharmaceutically relevant backbone on which to build selective clinically valuable agents.

Fig. 4
figure4

28 and ent-28 are selective agonists of ERβ. a ERβ and ERα agonist assays (Z’ = 0.67 for ERβ Z’ = 0.77 for ERα, see Supplementary Information for experimental details). b 28 and ent-28 are agonists of ERβ in human DU-145 prostate cancer cells (17β-estradiol, 28, and ent-28 were evaluated at 5 µM). Error bars represent standard deviation of the mean (n = 6 per group), y-axis in OD 405 nM. Source data are provided as a Source Data File

Regulation of ERβ in prostate cancer cells

Prostatic epitheleal hyperplasia is evident in mice lacking ERβ50, suggesting that ERβ may play a role in limiting proliferation in the prostate. These data suggest that pharmacological activation of ERβ may be useful in treatment of prostate cancer. The DU-145 prostate cancer cell line only expresses ERβ51, and can be used as a model to examine the activation of ERβ in the absence of any potential confounding signal driven by ERα. Alkaline phosphatase (ALP) expression is induced in an ER-dependent manner, and has been used to characterize the activity of synthetic ER agonists and antagonists52.

In order to examine the ability of 28 and ent-28 to activate ERβ in the context of a cell line expressing endogenous ERβ, DU-145 cells were treated for 24 h with either 17β-estradiol, 28, or ent-28, and ALP activity derived from the cells was examined. As shown in Fig. 4b, 17β-estradiol was effective in inducing ALP activity as expected. In addition, both 28 and ent-28 were effective at inducing ALP to levels comparable to 17β-estradiol. These data are consistent with 28 and ent-28 functioning as ERβ agonists.

Inhibition of glioblastoma in vitro

Glioblastomas are the most malignant and lethal brain tumors, with a median survival rate of 14 months and a 5-year survival rate of less than 10%. Despite attempts to combine surgery, radiation, and chemotherapeutics, gliomas recur in more than 90% of cases. This is primarily due to gliomas being heterogeneous tumors with each individual tumor expressing distinct patterns of oncogenes and mutated tumor-suppressor genes, thus making molecular characterization and targeted treatment challenging. However, elevated levels of ERβ expression have been observed in most glioblastomas examined to date33,53. ERβ is an established tumor suppressor in several cancers; higher expression of ERβ is correlated to a better prognosis, and ERβ agonists induce apoptosis53,54,55. Nevertheless, despite the tumor suppressive role of ERβ, 17β-estradiol (a potent but unselective agonist of ERα and ERβ) is not used as a therapy against gliomas because long-term treatment can result in cancers of the female reproductive system and prostate cancer in men56,57. In comparison with 17β-estradiol, 28 and ent-2858 have a unique profile for selective agonism of ERβ (Fig. 4a). Furthermore, as data from both the ERβ specific in vitro bioluminescence reporter assay (Fig. 4a) and the ERβ only expressing prostate cancer line DU-145 (Fig. 4b) established that 28 and ent-28 are ERβ agonists, we hypothesized that targeting ERβ with 28 or ent-28 in gliomas may establish a novel and highly efficacious therapy against this deadly brain tumor.

To determine the biological effect of 28 and ent-28, human glioblastoma-derived cell lines U251 and U87 were treated with varying concentrations of 28, ent-28, temazolomide (TMZ; a standard of care chemotherapeutic agent), or vehicle-toxicity control, dimethylsulfoxide (DMSO) used at final volumes that were identical to those used to attain all concentrations tested of 28 and ent-28. Remarkably, 28 and ent-28 demonstrated inhibition of glioma cell growth (IC50) at a 50-fold lower concentration when compared with TMZ after 24 h (Fig. 5a). Furthermore, when cell viability was assessed every 24h for 5 days, we observed that 28 and ent-28 reduced viability and the resulting proliferation of glioblastoma lines in a dose-dependent manner compared with cells that were incubated with TMZ or DMSO (Fig. 5a; Supplementary Fig. 1). More importantly, cell viability of human neural stem cells as well as human astrocytes was not affected at the equivalent concentration, demonstrating that both 28 and ent-28 have glioma-specific cytotoxic activity (Fig. 5; Supplementary Figs. 2, 3). This was further confirmed by MTT cell-viability assays wherein 28 and ent-28 treatment significantly reduced the growth of glioblastoma cell lines in a dose-dependent manner (Supplementary Figs. 4, 5).

Fig. 5
figure5

Ent-28 and 28 inhibit proliferation and induce apoptosis in glioblastoma cell lines. a IC50 at 24 h: dose-dependent growth inhibition of U251 and U87 cells treated with 28, ent-28, TMZ, and vehicle control DMSO (n = 4). b Cell proliferation over 96 h: viable cell counts of U251 and U87 cells treated with 28, ent-28, TMZ, and vehicle control DMSO determined every 24 h for 96 h (n = 4). c Apoptosis at 24 h: flow-cytometric analysis of U251 and U87 co-stained with Annexin V-FITC/7-AAD after treatment with 28, ent-28, TMZ, and vehicle control DMSO (n = 4). d Morphological analyses at 24 h: phase-contrast images using Incucyte for live cell tracking of U251 and U87 cells in response to treatment with 28, ent-28, TMZ, and vehicle control DMSO. Live cells were tracked and imaged for 96 h (data not shown). Scale bar = 300 μm. All data in this figure are representative of four independent experiments, and four replicates of each treatment condition were analyzed per experiment. Mean ± s.d. of quadruplicates from one experiment are presented on the line plots. Source data are provided as a Source Data File

In order to elucidate the mechanism of this cell death, glioblastoma cells treated for 24 h with 28, ent-28, TMZ, or DMSO control were analyzed for early- and late-apoptosis markers (Annexin V and 7-Aminoactinomycin D) by FACS analyses. These experiments revealed a sevenfold higher induction of apoptosis in U251 and U87 cells treated with 28 or ent-28 compared with cells treated with either TMZ or DMSO controls (Fig. 5c; Supplementary Fig. 6). In addition, over a period of 5 days, morphological changes in U251 and U87 cells treated with 28 and ent-28 were tracked using the Incucyte live cell imaging system. Increased loss of adherence and cellular integrity were observed following treatment of U251 and U87 with 28 and ent-28, correlating with the decreased viability and increased cell death observed previously; similar behavior was not seen with human neural stem cells as well as human astrocytes (Fig. 5d; Supplementary Figs. 2, 3).

Collectively, these investigations indicate that 28 and ent-28 each reduce cell viability, decrease survival, and induce apoptosis selectively in glioblastoma cells. This profile supports further investigation of the novel enantiomeric tetracycles 28 and ent-28 as therapeutic agents against gliomas that are resistant to all currently available treatment modalities.

In conclusion, the rich and diverse collection of tetracyclic terpenoids has served as motivation for the design and execution of a new chemical synthesis platform capable of constructing central motifs of their carbocyclic backbones in just a handful of steps from an inexpensive and readily available chiral starting material (epichlorohydrin). The process comprises a modern metallacycle-mediated annulative cross-coupling, a C9–C10 bond-forming process through a double-asymmetric Friedel–Crafts cyclization, and an oxidative rearrangement reaction. It has been shown that this sequence of molecular transformations can be used to deliver a variety of stereodefined systems that contain quaternary centers at C9, C10, C13, and C14, and that compositions of matter encountered in this chemical synthesis pathway are potent agonists of ERβ and have selective anticancer properties against glioblastoma. While it is anticipated that molecules now accessible from this chemical advance will be a rich source of bioactive leads for drug discovery and development across a diverse therapeutic landscape, it is already clear that the science described allows for de novo synthesis of either enantiomer of the tetracyclic skeletons resident in a wide variety of terpenoids with ease, rendering unique opportunities for medicinal exploration viable. Efforts directed at advancing this collection of advances in preparative organic chemistry to address challenges in natural product synthesis and medicinal science are of great current and future interest.

Methods

Synthesis of enyne 22

To a stirring solution of 21 (5.4 g, 31 mmol, 2.0 equiv) in 90 mL of THF at −78 °C under N2 atmosphere was added n-BuLi (2.5 M in hexanes, 10 mL, 25 mmol, 1.6 equiv) dropwise over 3 min, and the resulting mixture was stirred for 30 min at the same temperature. After the specified period of time, BF3•OEt2 (4.0 g, 28 mmol, 1.8 equiv) was added dropwise to the reaction mixture followed by 7 (1.5 g, 15 mmol, 1.0 equiv). The resulting mixture was stirred at −78 °C under N2 atmosphere for 55 min, and then quenched with 50 mL of saturated solution of NaHCO3 at the same temperature. The resulting biphasic solution was warmed to room temperature, and then further diluted with 50 mL of ethyl acetate. The organic layer was separated, dried over Na2SO4, filtered, and then the filtrate was concentrated in vacuo. SiO2 flash column chromatography afforded the title compound 22 as a clear oil (3.9 g, 91%); Spectral Data for 22: 1H NMR (600 MHz, Chloroform-d): δ 7.13 (t, J = 7.9 Hz, 1H), 6.85 (d, J = 1.1 Hz, 1H), 6.76 (d, J = 1.1 Hz, 1H), 4.92 – 4.86 (m, 1H), 4.86 – 4.80 (m, 1H), 3.89 – 3.84 (m, 1H), 3.83 (s, 3H), 2.87 (t, J = 7.7 Hz, 2H), 2.47 (tt, J = 7.6, 2.4 Hz, 2H), 2.43 – 2.33 (m, 2H), 2.29 (ddd, J = 13.9, 4.9, 1.2 Hz, 1H), 2.25 – 2.18 (m, 4H), 2.16 (s, 1H), 1.79 (s, 3H); 13C NMR (150 MHz, chloroform-d): δ 157.7, 142.4, 140.1, 126.0, 124.5, 121.4, 113.3, 108.3, 82.3, 76.8, 67.8, 55.4, 44.7, 32.9, 27.2, 22.5, 19.8, 11.2; IR (thin film): 3441, 2933, 2835, 1585, 1463, 1439, 1258, 1101 cm;−1 HRMS (ESI–TOF) calculated for C18H25O2 [M+H]+ 273.1855, found 273.1855;\([\alpha ]_{\mathrm{D}}^{22} = - 1.1\) (0.05, CHCl3).

Synthesis of hydrindane 23

To a stirring solution of 1-(trimethylsilyl)propyne (3) (4.5 g, 40 mmol, 3.1 equiv) and Ti(Oi-Pr)4 (11 g, 38 mmol, 3.0 equiv) in 200 mL of anhydrous toluene at −78 °C under N2 atmosphere was added n-BuLi (2.3 M in hexanes, 33 mL, 76 mmol, 5.9 equiv) dropwise. After the addition, the cooling bath was removed, and the resulting dark brown mixture was warmed to rt, and then further warmed to 50 °C. The reaction mixture was stirred for 50 min at the same temperature without a reflux condenser, and then cooled to rt. A separate round bottom flask charged with a solution of 22 (3.5 g, 13 mmol, 1.0 equiv) in 50 mL of anhydrous toluene at −78 °C under N2 atmosphere was added n-BuLi (2.3 M in hexanes, 5.5 mL, 13 mmol, 1.0 equiv) dropwise. The resulting solution was warmed to rt, cannulated into the above dark brown mixture, and then stirred overnight at rt under N2 atmosphere (approx. 12 h). After this period, 100 mL of saturated solution of NH4Cl was added, and the mixture was further diluted with 100 mL of ethyl acetate. The organic layer was separated, and the aqueous layer was extracted with 250 mL × 2 ethyl acetate. The combined organic layers were dried with Na2SO4, filtered, and the filtrate was concentrated in vacuo. SiO2 flash column chromatography afforded the title compound 23 as a yellow oil (2.6 g, 53% isolated yield); Spectral Data for 23: 1H NMR (500 MHz, chloroform-d): δ 7.07 (t, J = 7.9 Hz, 1H), 6.71 (d, J = 7.9 Hz, 2H), 4.41 – 4.31 (m, 1H), 3.81 (s, 3H), 2.73 – 2.62 (m, 2H), 2.57 (dt, J = 13.5, 8.0 Hz, 1H), 2.44 – 2.37 (m, 1H), 2.35 – 2.27 (m, 1H), 2.20 (s, 3H), 2.16 (d, J = 15.5 Hz, 1H), 2.05 – 1.97 (m, 3H), 1.95 (app d, J = 2.7 Hz, 3H), 1.40 (dd, J = 12.4, 7.5 Hz, 1H), 1.20 (s, 1H), 0.79 (s, 3H), 0.16 (s, 9H); 13C NMR (150 MHz, Chloroform-d): δ 157.8, 144.4, 141.8, 141.3, 129.5, 128.5, 126.0, 124.8, 122.3, 108.0, 72.1, 55.7, 51.3, 41.6, 39.4, 38.5, 33.2, 30.5, 21.4, 19.2, 11.4, 0.2; IR (thin film): 3349, 2950, 1585, 1465, 14371102, 1063, 1063, 835 cm;−1 HRMS (ESI–TOF): calculated for C24H37O2Si [M+H]+ 385.2563, found 385.2563; \([\alpha ]_{\mathrm{D}}^{22} = - 39.3\) (c 0.014, CHCl3).

Synthesis of tetracycle 24

To a stirring suspension of (S)-BINOL (6.1 g, 21 mmol, 5.3 equiv) in 200 mL of dichloromethane at −78 °C under N2 atmosphere was added a solution of SnCl4 (1.0 M in dichloromethane, 21 mL, 21 mmol, 5.3 equiv) dropwise using syringe. The resulting mixture was stirred for 29 min at −78 °C, and then a solution of 23 (1.5 g, 4.0 mmol, 1.0 equiv) in 80 mL of dichloromethane was added dropwise via cannula transfer. The resulting mixture was stirred for 1.3 hr at −78 °C, and then warmed to rt over 30 min. The reaction was judged to be complete by TLC-analysis, and 100 mL of saturated solution of NH4Cl was added. The organic layer was separated, washed with 200 mL 5% solution of NaOH. The aqueous layer was further extracted with 200 mL of dichloromethane. The combined organic layers were dried over Na2SO4, filtered, and the filtrate was concentrated in vacuo. SiO2 flash column chromatography afforded the title compound 24 as an amorphous white solid (0.96 g, 78%); Spectral Data for 24: 1H NMR (600 MHz, chloroform-d) δ 7.14 (d, J = 8.7 Hz, 1H), 6.77 (d, J = 8.7 Hz, 1H), 4.63 – 4.57 (m, 1H), 3.80 (s, 3H), 2.92 (dd, J = 14.5, 5.7 Hz, 1H), 2.85 (dd, J = 16.7, 8.7 Hz, 1H), 2.54 – 2.46 (m, 2H), 2.38 – 2.27 (m, 2H), 2.16 (dd, J = 11.9, 6.7 Hz, 1H), 2.13 – 2.05 (m, 4H), 1.85 – 1.78 (m, 1H), 1.71 (dt, J = 9.3, 3.1 Hz, 2H), 1.57 (s, 1H), 1.38 – 1.32 (m, 4H), 0.89 (s, 3H); 13C NMR (150 MHz, chloroform-d) δ 155.0, 140.4, 135.9, 131.8, 124.1, 123.7, 108.7, 71.7, 55.7, 52.1, 41.5, 38.3, 37.7, 35.0, 33.6, 31.6, 29.7, 25.9, 24.8, 11.5; IR (thin film): 3344, 2934, 2856, 2833, 1595, 1482, 1263, 1101, 755; HRMS (ESI–TOF): calculated for C21H29O2 [M+H]+ 313.2168, found 313.2162; \([\alpha ]_{\mathrm{D}}^{22} = - 169.71\) (c 0.0046, CHCl3).

Synthesis of enone 25

To a stirring solution of 24 (1.5 g, 4.7 mmol, 1.0 equiv) in 50 mL of anhydrous toluene at rt under N2 atmosphere was added DIBAL-H (1.0 M in hexanes, 47 mL, 47 mmol, 10 equiv). The resulting mixture was warmed to 100 °C, refluxed overnight (approx. 16 h), and then cooled to rt. Small chunks of ice was slowly added, and the resulting mixture was diluted with 200 mL water, 10 mL 1 M solution of HCl, and 250 mL of ethyl acetate. The separated organic layer was dried with Na2SO4, filtered, and concentrated in vacuo to afford 1.41 g of the crude product as an amorphous yellow solid. The crude product (1.4 g) at 0 °C under N2 atmosphere was added with 50 mL of HFIP followed by PIDA (1.4 g, 4.3 mmol). The resulting mixture was stirred for 1 min at 0 °C (PIDA was fully dissolved at this point), and then 30 mL of saturated solution of NaHCO3 was added. HFIP was removed in vacuo, and the remaining aqueous mixture was extracted with 100 mL × 3 ethyl acetate. The combined organic layers were dried over Na2SO4, filtered, and the filtrate was concentrated in vacuo. The crude product was purified with SiO2 flash column chromatography to afford the title compound 25 as an amorphous white solid (0.76 g, 54% over 2 steps). Spectral data for 25: 1H NMR (500 MHz, chloroform-d) δ 7.14 (d, J = 10.1 Hz, 1H), 6.28 (d, J = 10.1 Hz, 1H), 5.50 (s, 1H), 5.11 – 5.02 (m, 1H), 2.97 (ddd, J = 13.0, 6.0, 1.7 Hz, 1H), 2.71 (ddt, J = 16.5, 6.3, 2.0 Hz, 1H), 2.53 – 2.39 (m, 2H), 2.34 (app dd, J = 12.1, 6.5 Hz, 2H), 2.18 (ddtd, J = 18.6, 10.3, 4.1, 2.0 Hz, 1H), 1.95 (s, 3H), 1.82 (ddd, J = 12.6, 5.0, 1.4 Hz, 1H), 1.64 (s, 1H), 1.57 (td, J = 12.4, 5.6 Hz, 2H), 1.45 (s, 3H), 1.40 (dd, J = 12.1, 7.6 Hz, 1H), 0.88 (s, 3H); 13C NMR (150 MHz, chloroform-d) δ 185.4, 159.4, 151.9, 149.9, 137.9, 128.3, 127.6, 125.4, 124.3, 76.6, 51.7, 44.8, 43.3, 36.2, 28.8, 28.4, 25.4, 24.7, 23.6, 10.5; IR (thin film): 3404, 2923, 2851, 1659, 1625,1607, 1449, 1051, 833, 753 cm;−1 HRMS (ESI–TOF): calculated for C20H25O2[M+H]+ 297.1855, found 297.1856; \([\alpha ]_{\mathrm{D}}^{22} = + 156.27\) (c 0.0032, CHCl3).

Synthesis of tetracycle 26

To a stirring solution of S7 (2.9 g, 9.3 mmol, 1.0 equiv) and water (3.6 g, 200 mmol, 22 equiv) in 190 mL dimethoxyethane was added Co2(CO)8 (8.0 g, 23 mmol, 2.5 equiv). The resulting mixture was refluxed for 3 h at 90 °C, and for 1 h at 100 °C. The reaction mixture was cooled to rt, and another portion of Co2(CO)8 (3.2 g, 9.4 mmol, 1.0 equiv) was added. The reaction mixture was warmed to 90 °C again, continued to reflux for 1.7 h, and then cooled to rt. The resulting mixture was passed through a pad of silica gel using 850 mL of ethyl acetate, 100 mL 5% methanol/95% ethyl acetate, and 10% methanol/90% ethyl aceate as the eluent, and then the filtrate was concentrated in vacuo. The crude product was purified by dry column vacuum chromatography1 using 200 mL of dichloromethane and then a gradient elution (20% ethyl acetate/80% hexanes to 50% ethyl aceate/50% hexanes, 5% increase in ethyl acetate/ fraction, 500 mL/fraction) to afford 2.02 g of the title compound 26 as an amourphous white solid (69% yield). Spectral data for 26: 1H NMR (600 MHz, chloroform-d) δ 4.63 (td, J = 7.7, 4.0 Hz, 1H), 2.72 (ddd, J = 12.9, 4.9, 2.3 Hz, 1H), 2.51 (ddd, J = 17.3, 14.7, 5.1 Hz, 1H), 2.42 (ddd, J = 17.3, 4.8, 2.7 Hz, 1H), 2.24 – 2.07 (m, 4H), 1.94 (dt, J = 7.4, 3.5 Hz, 1H), 1.86 – 1.66 (m, 7H), 1.65 – 1.58 (m, 3H), 1.30 (s, 3H), 0.96 (dd, J = 12.5, 8.7 Hz, 1H), 0.88 (s, 3H), 0.82 (dd, J = 5.1, 3.1 Hz, 1H), 0.38 (dd, J = 7.5, 5.2 Hz, 1H); 13C NMR (150 MHz, chloroform-d) δ 198.7, 163.5, 133.3, 129.5, 127.4, 73.7, 41.4, 40.0, 38.9, 37.7, 34.0, 33.4, 31.6, 26.33, 26.26, 26.1, 23.4, 23.0, 21.7, 10.8, 10.1; IR (thin film): 3399, 2922, 1660, 1613, 1450, 1373, 1357, 1332, 1074, 1054, 1025 cm;−1 HRMS: calculated for C21H29O2 [M+H]+ 313.2168, found 313.2164; \([\alpha ]_{\mathrm{D}}^{22} = + 252.2\) (c 0.021, CHCl3).

Synthesis of tetracycle 27

To a stirring blue solution of Li (20 mg, 2.9 mmol, 10 equiv) in 10 mL NH3 (l) at −78 °C under N2 atmosphere was added a solution of 26 (89 mg, 0.28 mmol, 1.0 equiv) in 2 mL THF dropwise. The resulting mixture was stirred for 15 min at −78 °C, warmed to −35 °C, and then stirred for 50 min while maintaining the cold bath temperature between −35 °C and −25 °C. After this period, the reaction mixture was cooled to −78 °C, and then MeI (0.81 g, 5.7 mmol, 20 equiv) was added dropwise. The cold bath was removed, and NH3 (l) was evaporated under a stream of N2 over 30 min. In total, 5 -mL saturated solution of NH4Cl was added, and the resulting mixture was further diluted with 10 mL of ethyl acetate and 5 mL of water. The organic layer was separated, and the aqueous layer was extracted with 25 mL × 2 ethyl acetate. The combined organic layers were dried with Na2SO4, filtered, and the filtrate was concentrated in vacuo. SiO2 flash column chromatography afforded the title compound 27 as a pale yellow film (48 mg, 52%); Spectral data for 27: 1H NMR (500 MHz, chloroform-d) δ 4.69 – 4.59 (m, 1H), 2.57 (ddd, J = 15.9, 10.4, 7.5 Hz, 1H), 2.46 (ddd, J = 15.9, 7.5, 3.8 Hz, 1H), 2.20 – 2.07 (m, 2H), 2.07 – 2.00 (m, 1H), 1.94 (dt, J = 7.4, 3.5 Hz, 1H), 1.81 – 1.71 (m, 1H), 1.71 – 1.56 (m, 7H), 1.45 (dq, J = 18.4, 6.2 Hz, 1H), 1.36 (br s, 1H), 1.09 (s, 3H), 1.08 (s, 3H), 1.05 (s, 3H), 0.97 – 0.90 (m, 4H), 0.75 (dd, J = 5.0, 3.1 Hz, 1H), 0.32 (dd, J = 7.5, 5.0 Hz, 1H); 13C NMR (150 MHz, chloroform-d) δ 217.9, 134.8, 128.0, 73.9, 51.7, 47.4, 41.7, 39.1, 38.1, 37.5, 35.5, 34.6, 31.9, 26.79, 26.76, 26.3, 22.9, 21.9, 21.3, 19.9, 19.7, 10.2; IR (thin film): 3392, 2950, 2924, 1704, 1473, 1457, 1383, 1058, 1020, 754 cm;−1 HRMS: calculated for C22H31O2 [M–H]+ 327.2324, found 327.2325; \([\alpha ]_{\mathrm{D}}^{22} = + 82.0\) (c 0.0065, CHCl3).

Cell lines and reagents

Human GBM cells U87, U251 were obtained from American Type Culture Collection (ATCC, Manassas, VA) and were cultured as per ATCC guidelines. Adult human neural progenitor cells were a generous gift from Drs. Steindler & Tong, Tufts University. Human astrocytes were obtained from ThermoFisher (Catalog number: N7805100). Following standard laboratory protocols, all study model cells utilized were determined to be free of mycoplasma contamination, and were confirmed by using MycoSEQ™ Mycoplasma Detection Kit, with Discriminatory Positive Control (ThermoFisher; Catalog number: 4460623).

Cell survival/proliferation and IC50 assays

To determine growth inhibition, U87 and U251 cells plated in 96-well plates for 24 h were treated with varying doses of 28, ent-28, TMZ or vehicle control DMSO for 96 h. Cells were counted every 24 h after initiating treatment. This was done after the wells were first washed with PBS and the remaining adherent, viable cells in each well were trypsinized and counted using a hemocytometer following trypan blue exclusion. The data were normalized to untreated cell counts obtained from cells prior to initiating treatment at 0 h. The data were analyzed from four replicates and each experiment was repeated at least three times.

Flow-cytometric analysis of apoptotic cell death

U87 and U251 cells were seeded in six-well plates (300,000 cells/well) 24 h prior to treatment. Each well was treated with various concentrations of 28, ent-28, TMZ, or vehicle control DMSO for 24 h. DMSO was used as a negative control. The cells were then washed and stained with Annexin V-FITC and 7-AAD (BioLegend), according to the manufacturer’s instructions, and were analyzed on a MACSQuant® Analyzer 10 (Miltenyi Biotec Inc.). Staurosporine (STS) treatment at 1 μM served as a positive control for Annexin V staining and DMSO was used as a negative control. The files were converted to FCS compatible format and analyzed using FlowJo analysis software (Treestar). The quantitative analysis of the percentage of apoptotic cells was reported from three independent experiments.

Cell morphology studies of 28 and ent-28 versus TMZ

U87 and U251 cell lines, adult human neural progenitor cells (a generous gift from Drs. Steindler & Tong, Tufts University), and human astrocytes (ThermoFisher) were seeded in a 96-well plate format with a density of 1000 cells per well and incubated for 24 h before treatment. Each well was then supplemented with fresh media containing various concentrations of 28, ent-28, TMZ, or vehicle control DMSO. Phase-contrast images at ×10 magnification were acquired using the IncuCyte® Live-Cell Imaging System (Essen Instruments). Each well was imaged four times every 24 h over 5 days and image-based analysis of cell morphology was carried out using the Incucyte Software. The results are representative of four independent experiments.

Cell-viability assays

Cell viability after treatment in quadruplicate in 96-well plates was detected by the MTT assay. U87 and U251 cells plated as described above were treated with 28, ent-28, and TMZ with a DMSO control over 96 h. Every 24 h, the medium was removed and 20 μL of MTT (5 mg/mL) was added for 4 h. After removing supernatant, 100 μL of DMSO was added to resolve formazan crystals, and the plates were measured at 595 nm with a SpectraMax® i3x microplate reader (Molecular Devices, Sunnyvale, CA, USA). The data were collected using Softmax Pro 6.5.1 software (Molecular Devices). The results are representative of at least three independent experiments.

Evaluation of 28 and ent-28 in DU-145 prostate cancer cells

Human DU-145 prostate cancer cells were plated at a density of 10,000 cells per well (96-well plates) in RPMI + 10% fetal bovine serum (FBS) without phenol red. Two days after plating, cells were treated with compounds (5 µM) for 24 h in RPMI + 10% charcoal-stripped FBS without phenol red. ALP activity was determined using the ThermoFisher Scinetific 1-step PNPP assay according to the manufacturer’s protocol.

Reporting summary

Further information on research design is available in the Nature Research Reporting Summary linked to this article.

Data availability

All relevant data are available from the authors, and are included with the manuscript in the accompanying Supplementary Information. These include characterization for all new compositions of matter (organic molecules), as well as copies of relevant NMR spectra, experimental procedures, and supplemental figures associated with the biological evaluation of 28 and ent-28 (data associated with these evaluations are provided in the Manuscript and Supplementary Information; raw data is available upon request). X-ray crystallographic coordinates for compound 24 have been deposited at the Cambridge Crystallographic Data Centre (CCDC), under deposition number CCDC 1897943. These data can be obtained free of charge from The Cambridge Crystallographic Data Centre via www.cdc.cam.ac.uk/data_request/cif. The raw data underlying Figs. 4 and 5, as well as Supplementary Figs. 1, 2, 4, and 5 are provided in a Source Data File.

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Acknowledgements

We gratefully acknowledge financial support of this work by the National Institutes of Health—NIGMS (GM080266), and the Early Phase Trials—Clinical Oncology Group at the Norris Cotton Cancer Center (EPT-COG at NCCC). We also acknowledge Indigo Biosciences for performing the ERα and ERβ agonism assays depicted in Fig. 4a.

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W.S.K., Z.A.S., and G.C.M. contributed to the chemical experiments, W.S.K. and Z.A.S. performed all chemical reactions reported, E.S. and T.P.B. conducted the evaluation of 28 and ent-28 in human DU-145 cells, S.M.N. and A.B.G. designed and conducted the in vitro evaluation of 28 and ent-28, A.E. performed an early in vitro evaluation of the toxicity of 28 and ent-28 in glioblastoma, and G.C.M. wrote the paper with contributions from all authors.

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Correspondence to Arti B. Gaur or Glenn C. Micalizio.

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Kim, W.S., Shalit, Z.A., Nguyen, S.M. et al. A synthesis strategy for tetracyclic terpenoids leads to agonists of ERβ. Nat Commun 10, 2448 (2019). https://doi.org/10.1038/s41467-019-10415-6

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