HIV persists in latently infected CD4+ T cells during antiretroviral therapy (ART). Immune checkpoint molecules, including PD-1, are preferentially expressed at the surface of persistently infected cells. However, whether PD-1 plays a functional role in HIV latency and reservoir persistence remains unknown. Using CD4+ T cells from HIV-infected individuals, we show that the engagement of PD-1 inhibits viral production at the transcriptional level and abrogates T-cell receptor (TCR)-induced HIV reactivation in latently infected cells. Conversely, PD-1 blockade with the monoclonal antibody pembrolizumab enhances HIV production in combination with the latency reversing agent bryostatin without increasing T cell activation. Our results suggest that the administration of immune checkpoint blockers to HIV-infected individuals on ART may facilitate latency disruption.
Latently infected cells carrying integrated human immunodeficiency virus (HIV) genomes persist during antiretroviral therapy (ART) and represent the main barrier to a cure1,2,3. The establishment of latency may result from direct infection of resting CD4+ T cells4 or from infection of CD4+ T cells transitioning from an activated to a resting state5. Latently infected CD4+ T cells are rare both before and after ART initiation6,7, suggesting that HIV latency is established only in a small fraction of CD4+ T cells.
Programmed cell death-1 (PD-1) is an immune checkpoint molecule expressed at high levels on the surface of exhausted HIV-specific CD4+ and CD8+ T cells8,9,10,11,12. Its blockade enhances CD4+ T cells and CD8+ T cells functions during Simian immunodeficiency virus infection13,14.
In addition to its role in T-cell exhaustion, PD-1 and other immune checkpoint molecules such as lymphocyte activation gene-3 (LAG-3) and T-cell immunoreceptor with Ig and ITIM domains (TIGIT) are preferentially expressed at surface of persistently infected CD4+ T cells15,16,17. Of note, follicular helper T (Tfh) cells, which express high levels of PD-1, are major producers of viral particles in untreated HIV infection18 and serve as a preferential reservoir for HIV during ART19,20. In addition, PD-1 and LAG-3 measured prior to ART strongly predict time to return of viraemia upon treatment interruption21. However, whether these molecules play an active role in the establishment and maintenance of HIV latency remains unclear.
In an in vitro latency model, PD-1 blockade reduces the frequency of latently infected CD4+ T cells22. Because PD-1 induces T-cell quiescence and inhibits T-cell activation23, we hypothesized that the engagement of the PD-1 pathway may directly contribute to the establishment of viral latency by inhibiting viral transcription and production. We demonstrate that the engagement of PD-1 abrogates T-cell receptor (TCR)-induced HIV reactivation in latently infected cells isolated from HIV-infected individuals. Conversely, PD-1 blockade with the monoclonal antibody pembrolizumab enhances HIV production induced by the latency reversing agent bryostatin without increasing T-cell activation. These results suggest that the administration of immune checkpoint blockers to HIV-infected individuals on ART may facilitate latency reversal in vivo.
PD-1 marks HIV-infected cells in viremic individuals
To determine if PD-1 could play a role in the establishment of HIV latency, we first assessed the distribution of HIV in memory CD4+ T cells expressing high and low levels of PD-1 in HIV-infected individuals not receiving ART. We found that memory CD4+ T cells expressing PD-1 were preferentially infected, as demonstrated by the higher frequency of integrated HIV DNA in PD-1 expressing central (TCM), transitional (TTM), and effector memory (TEM) cells as compared to their PD-1 negative (PD-1−) counterparts (median fold-change: 6.5, 2.3, and 2.2, respectively, Supplementary Fig. 1a). Accordingly, flow cytometry sorted PD-1 positive (PD-1+) cells produced higher levels of viral particles, indicating that PD-1+ cells are major targets for productive HIV infection during untreated disease (Supplementary Fig. 1b).
PD-1 engagement inhibits viral production
To determine the impact of PD-1 engagement on HIV production, we stimulated productively infected CD4+ T cells isolated from untreated HIV-infected individuals in the presence or absence of PD-L1, one of the two ligands for PD-1. TCR stimulation led to a marked increase in the amount of the viral protein p24 measured in the culture supernatant and this induction was dramatically reduced in the presence of PD-L1 (98% inhibition, p < 0.0001, Fig. 1a and Supplementary Fig. 1c). Of note, this effect was maintained over time, since the inhibition was still observed after 6 and 9 days of culture (Fig. 1b, c and Supplementary Fig. 1d). Importantly, PD-L1 acted directly on productively infected cells and not by decreasing the permissiveness of bystander cells since the inhibition of viral production was also observed in the presence of antiretroviral drugs (ARVs) that block new infection events as well as in short-term cultures (69–86% inhibition, Supplementary Fig. 1e–g). To demonstrate the specificity of this mechanism, we sorted PD-1+ and PD-1− memory CD4+ T cells and observed that PD-L1− mediated inhibition was restricted to PD-1+ cells (p = 0.01), since the ligand did not significantly affect viral production in PD-1− cells (Fig. 1d).
To gain further insights into the mechanism by which PD-1 engagement inhibited HIV production, we transfected primary CD4+ T cells with an LTR-luciferase reporter construct. The engagement of PD-1 by PD-L1 resulted in a significant reduction in the LTR activity, indicating that PD-1 exerted its inhibitory effect on HIV production at the transcriptional level (p = 0.002, Fig. 1e).
PD-1 inhibits viral reactivation in latently infected cells
Having demonstrated that the engagement of PD-1 at the surface of productively infected cells inhibited the viral production induced by TCR stimulation, we next sought to determine if this phenomenon could abrogate viral reactivation from latently infected cells. Even after years of ART, PD-1 expression at the surface of CD4+ T cells from virally suppressed individuals was not normalized (Supplementary Fig. 2a), notably in the TTM and TEM subsets (Supplementary Fig. 2b–e). Similarly, both PD-1 ligands (PD-L1 and PD-L2) were expressed at higher levels at the surface of CD4+ T cells and monocytes from fully suppressed individuals when compared to uninfected controls (Supplementary Fig. 2f–i). Of note, higher frequencies of CD4+ T cells expressing PD-1 were detected in the rectum, colon, and terminal ileum of individuals on ART when compared to matched blood and lymph nodes (Supplementary Fig. 2j). These higher frequencies of CD4+ T cells expressing PD-1 was consistent with heightened frequencies of differentiated memory CD4+ T cells (TTM and TEM) in the gut compartment (Supplementary Fig. 2k). We further confirmed our and others previous observations16,19,24 indicating a strong positive correlation between the frequency of persistently infected CD4+ T cells (as measured by integrated HIV DNA) and PD-1 expression in CD4+ T cells during ART (r = 0.42, p < 0.0001, Supplementary Fig. 3a) as well as the preferential infection of PD-1+ TCM and TTM cells during ART by measuring integrated HIV DNA (median fold-enrichment: 1.7, p = 0.01 and 1.6, p = 0.04, respectively, Supplementary Fig. 3b). In addition, using the tat/rev induced limiting dilution assay (TILDA) and quantitative viral outgrowth assay, we observed that inducible and replication-competent proviruses were enriched in CD4+ T cells expressing PD-1 in the majority of the samples tested (Supplementary Fig. 3c, d).
To assess the effect of PD-1 engagement on viral reactivation from latency, we stimulated CD4+ T cells from virally suppressed individuals in the presence or absence of PD-L1. TCR stimulation led to readily detectable levels of viral production that were dramatically reduced in the presence of PD-L1 (89% inhibition, p < 0.0001, Fig. 2a), demonstrating that PD-1 engagement inhibits TCR-induced reactivation in latently infected CD4+ T cells. This inhibition was observed at the transcriptional level and restricted to PD-1+ cells (Supplementary Fig. 4a, b). Similarly, PD-1 engagement inhibited TCR-induced activation of the positive transcription elongation factor b (P-TEFb), a master regulator of HIV transcription (82% inhibition of CDK9 phosphorylation, p = 0.01, Fig. 2b and 61% inhibition of cyclin T1 expression, p = 0.02, Fig. 2c)25.
PD-1 blockade enhances viral production ex vivo
Since our results indicated that PD-1 engagement prevents HIV reactivation from latency, we sought to determine if PD-1 blockade could facilitate latency reversal. We stimulated large numbers of CD8- and CD56-depleted peripheral blood mononuclear cells (PBMCs) from HIV-infected individuals on suppressive ART with the latency reversing agent bryostatin in the presence of a PD-1 blocking antibody (pembrolizumab) or the corresponding isotype control. HIV production was greatly increased by PD-1 blockade (median fold-increase over isotype control (interquartile range (IQR)) 36.00 (7.90–47.25), Fig. 2d) without enhancing T-cell activation nor affecting cell viability (Fig. 2e). Of note, blocking PD-1 also enhanced viral production when latently infected CD4+ T cells were stimulated with suboptimal concentrations of the superantigens Staphylococcal Enterotoxins A and B (SEA and SEB) (median fold-increase over isotype control (IQR) 4.23 (2.04–71.96), Supplementary Fig. 5a). Interestingly, PD-1 blockade significantly increased the levels of cytokine production (Supplementary Fig. 5b, c) but had minimal effects on markers of T-cell homeostasis, proliferation, survival and activation upon TCR or IL-7 stimulations (Supplementary Fig. 6a–g). Together, these experiments indicated that blocking PD-1 increases viral production from latently infected cells in response to the latency reversing agent bryostatin and to suboptimal TCR stimulation without enhancing T-cell activation.
Our in vitro findings were further supported by the progressive decrease in total and integrated HIV DNA as well as in cell-associated HIV RNA (4-, 3-, and 19-fold decreases, respectively) observed in a virally suppressed individual who received pembrolizumab for metastatic melanoma (Supplementary Fig. 7). We acknowledge that this case report does not prove latency reversal in vivo since a reduction in HIV persistence markers could result from multiple mechanisms, including immune-mediated effects. Nonetheless, this observation supports the concept that PD-1 blockade can reduce the latent reservoir in vivo.
Latently infected cells represent the major obstacle to a cure for HIV infection26. Memory CD4+ T cells are constantly exposed to homeostatic signals required for their maintenance (IL-7, IL-15, and perhaps interactions with self-peptide MHCII complex). However, the long half-life of memory CD4+ T cells and the modest activity of the majority of latency reversing agents27 suggest that inhibitory mechanisms may continuously abrogate viral reactivation in latently infected cells. Our results indicate that HIV preferentially persists in PD-1+ cells and that the engagement of PD-1 potently inhibits viral reactivation. It was recently reported that PD-1 suppresses T-cell function primarily by inactivating CD28 signaling28,29, which provides a potential explanation for the preferential persistence of HIV in PD-1+ TCM and TTM cells, two subsets that express high levels of CD28.
The ability of TCR signaling to enhance HIV transcription has been associated to release of P-TEFb from an inactive complex through an ERK-dependent pathway30. In this study, we provide mechanistic evidence that PD-1 engagement inhibits the P-TEFb signaling pathway, which likely contributes to the inhibition of HIV production. In addition to its immediate effect on HIV production, PD-1 engagement may also lead to a prolonged silencing of the HIV promoter through metabolic and epigenetic reprogramming of T cell31,32,33.
Importantly, our observations demonstrate that HIV reactivation is enhanced by PD-1 blockade in CD4+ T cells from virally suppressed individuals ex vivo. Of note, we observed that PD-1 blockade potentiates latency reversal without inducing global T-cell activation, in spite of increased levels of TCR-induced cytokine production. These observations suggest that cytokine secretion and HIV reactivation can be uncoupled from the induction of T-cell activation. This is in line with studies reporting that reduced levels of IL-2 production is sensitive hallmark of PD-1 engagement. Since NF-κB is an important factor positively regulating both IL-2 and HIV transcription, it is possible that activation of this transcription factor is involved in the increased levels of viral production following PD-1 blockade. As such, increased IL-2 secretion following PD-1 blockade was also reported in vivo34. Since the level of PD-1 expression on distinct CD4+ T-cell subsets impacts the effect of PD-1/PD-L1 blockade11, it remains to be determined if HIV latency can be reverted in all cellular HIV reservoirs using this strategy. Of note, since additional co-inhibitory receptors have been associated with HIV persistence in different subsets16,17,35 combination of immune-checkpoint blockers may be necessary to achieve optimal latency reversal.
We observed a progressive decline of several markers of HIV persistence throughout treatment with pembrolizumab in an individual receiving this antibody for malignant melanoma. This case illustrates the ability of PD-1 blockade to reduce the size of the HIV reservoir in some individuals, which is consistent with other studies demonstrating an effect of PD-1 or CTLA-4 blockade on HIV reservoir dynamics22,36,37. Since this effect is not observed in all virally suppressed individuals38, results from larger ongoing studies enrolling HIV-infected participants receiving immunotherapy for the treatment of malignancies are warranted.
The potential toxicity of these immunotherapies and the risk benefit ratio for HIV-infected individuals on stable suppressive ART should be considered carefully. To our knowledge, safety data in HIV-infected noncancer patients are not yet available. However, several reports in individuals with HIV and cancer38,39,40 indicate that the frequency of immune related adverse events due to PD-1 blockade in the setting of HIV appears to be similar to non-HIV. Of note, in all these studies including ours, the impact of PD-1 blockade on the HIV reservoir was assessed in peripheral blood. Knowing that CD4+ T cells expressing PD-1 are found at high frequencies in preferential anatomical HIV reservoirs such as gut, lymph nodes and adipose tissue during ART19,41,42, assessing latency reversal and HIV reservoir dynamics after PD-1 blockade administration would require analyzing tissues in which this process is more likely to occur and collecting samples shortly after administration of the PD-1 blocking antibody.
To conclude, our findings suggest that PD-1 may provide latently infected cells with a selective advantage to persist during ART by continuously inhibiting viral reactivation and provide the rationale for evaluating immune checkpoint blockers in curative strategies.
HIV-seropositive individuals on stable suppressive ART, chronically infected individuals with no history of ART and uninfected controls enrolled in this study. All participants signed inform consent approved by the Martin Memorial Health Systems (FL, USA), the UCSF (CA, USA), the Royal Victoria Hospital and the CHUM hospital (QC, Canada) review boards (IRB #10-1320, Ref # 068192 and FWA #00004139, respectively). The participant with metastatic malignant melanoma who received pembrolizumab-based immunotherapy was virally suppressed for more than 3 years and signed inform consent. Blood was collected 8 days after the second administration of the antibody and 20 to 40 days after all subsequent treatment cycles over a period of 4 months. We complied with all relevant ethical regulations for work with human participants.
PBMCs were isolated from peripheral blood and leukapheresis using Ficoll–Paque density gradient centrifugation. Single-cell suspensions from lymph node, rectum, colon, and ileal biopsies were prepared as previously described41,43. Cryopreserved PBMCs were thawed, washed and stained for phenotyping or cell sorting. To measure the expression of PD-1 in subsets of memory CD4+ T cells, the following antibody panel was used: CD3-Alexa700 (clone UCHT1, BD#557943), CD4-QDot605 (clone S3.5, Invitrogen#Q10008), CD8-PB (clone RPA-T8, BD#558207), CD14-V500 (clone M5E2, BD#561391), CD19-AmCyan (clone SJ25C1, BD#339190), LIVE/DEAD Aqua marker (Invitrogen#L34957), CD45RA-APC-H7 (clone HI100, BD#560674), CD27-BV650 (clone O323, Biolegend#302828), and CCR7-PE-Cy7 (clone 3D12, BD#557648) and PD-1-APC (clone MIH4, eBioscience#17-9969-42). Gates were defined using fluorescence minus one controls. CD4+ T-cell subsets were identified by CD27, CD45RA, and CCR7 expression on CD4+ T cells after exclusion of dump positive cells (LIVE/DEAD, CD14 and CD19). PD-1 was measured in gated CD4+ T-cell subsets including naïve CD4+ T cells (CD3+ CD8-CD4+ CD45RA+ CCR7+ CD27+), central memory CD4+ T cells (CD3+ CD8− CD4+ CD45RA− CCR7+ CD27+), transitional memory CD4+ T cells (CD3+ CD8− CD4+ CD45RA− CCR7− CD27+), effector memory CD4+ T cells (CD3+ CD8-CD4+ CD45RA− CCR7− CD27−) and terminally differentiated CD4+ T cells (CD3+ CD8-CD4+ CD45RA+ CCR7− CD27−). To measure the expression of PD-1 ligands (PD-L1 and PD-L2) the following antibody panel was used: CD3-PB (clone UCHT1, BD#558117), CD4-Alexa700 (cloneRPA-T4, BD#557922), CD8-PerCPCy5.5 (clone RPA-T8, BD#560662), CD14-FITC (clone M5E2, BD#555397), LIVE/DEAD Aqua marker (Invitrogen#L34957), CD45RA-APC-H7 (clone HI100, BD#560674), PD-1-APC (clone MIH4, eBioscience#17-9969-42), PD-L1-PE-Cy7 (clone MIH1, BD#558017), and PD-L2-PE (clone MIH18, BD#558066). Data were acquired on a BD LSR II flow cytometer using the FACSDiva software (Becton Dickinson) and analyzed using Flow Jo version 9 (Treestar).
Central, transitional and effector memory CD4+ T cells were sorted based on their expression of PD-1. The antibodies used for sorting were similar than those used for phenotyping (Supplementary Fig. 8). In a second set of experiments, total memory CD4+ T cells (CD3+ CD4+ CD45RA−) were sorted based on their expression of PD-1. Sorted cells were collected using a FACSAria II cell sorter (Becton Dickinson).
Isolation of total CD4+ T cells
Total CD4+ T cells were isolated from cryopreserved PBMCs using magnetic negative selection as per the manufacturer’s protocol (Stem Cell Technologies, Vancouver, Canada).
Quantification of integrated HIV DNA
Total CD4+ T cells or sorted CD4+ T-cell subsets were used to measure the frequency of cells harboring integrated HIV DNA by real time nested polymerase chain reaction (PCR)44. Briefly, cells were lysed by proteinase K digestion. Cell lysates were directly used for HIV DNA quantifications. Integrated HIV-1 DNA was amplified with two Alu primers together with a primer annealing the LTR/gag region (Supplementary Table 1). In all PCR reactions, primers specific for the CD3 gene were added to precisely quantify the cell input. In a second round of PCR, appropriate primers and probes were used to amplify HIV sequences from the first round of amplification. Inner primers specific for the CD3 gene were used in a separate reaction to determine cell input. The number of copies of integrated HIV-1 DNA was calculated by using serial dilutions lysed ACH-2 cells as a standard curve. Results were expressed as numbers of HIV copies per million cells.
Quantification of Tat/rev inducible multiply spliced HIV RNA
The frequency of CD4+ T cells with inducible multiply spliced HIV RNA was determined using the TILDA6. Briefly, cells were stimulated with phorbol myristate acetate (PMA; 100 ng/mL) and ionomycin (1 µg/mL) for 12 h. Serial dilutions of the stimulated cells (18,000; 9000; 3000, and 1000 cells; 24 replicates per dilution) were distributed in a 96 well plate containing real time PCR (RT-PCR) buffer. Multiply spliced HIV RNA was quantified by semi nested real time PCR using primers specific for the tat/rev region (Supplementary Table 1). The frequency of positive cells was calculated using the maximum likelihood method and this number was then expressed as a frequency of cells with inducible msHIV RNA per million cells.
Quantification of cell-associated and cell-free HIV RNA
Cell-associated RNA were extracted using the RNeasy kit (Qiagen). Alternatively, freshly collected cell culture supernatants were ultracentrifuged for 1 h at 25,000g to pellet HIV particles. By using this procedure, only packed viral RNAs were quantified, excluding HIV RNAs that are passively released in the medium as a consequence of cell death. Viral RNAs were extracted using the Qiamp viral RNA kit (Qiagen) and quantified using an ultrasensitive semi-nested real time RT-PCR with a detection limit of a single copy of HIV RNA per reaction45.
Quantification of viral production by p24 ELISA
Viral production was measured by quantification of the p24 antigen by a sensitive in-house p24 ELISA46. In this enzymatic assay, 183-H12-5C (coating) and 31-90-25 (conjugated to biotin) antibodies were used in combination to quantify p24 levels.
Quantification of replication-competent virus
Sorted memory PD1+ and PD1− CD4+ T cells were activated for 3 days with coated anti-CD3 (clone OKT3) and soluble anti-CD28 (clone CD28.2) in presence of anti-PD-1 antibody (pembrolizumab 10 µg/mL) in limiting dilution (from 1.5 million cells to 0.05 million cells). After 3 days of stimulation, cells were washed 2 times with RPMI and co-cultured for an additional 12 days with PHA/IL-2 activated CD4+ T cells from two HIV negative donors at a ratio 1:3 in medium containing IL-2 (5 ng/mL) and anti-PD-1 antibody (pembrolizumab 10 µg/mL, Merck). Culture were split twice weekly; half of cell culture supernatants were collected for quantification of the viral production by p24 ELISA. Infectious Unit per Million cells was determined based on the number of wells positive for p24 using the maximum likelihood method (http://silicianolab.johnhopkins.edu/).
CD4+ T cells were cultured in RPMI 1640 complemented with 10% fetal bovine serum and 1% penicillin–streptomycin in the presence or absence of ARVs [100 nM efavirenz (EFV), 180 nM zidovudine (AZT), and 200 nM raltegravir (RAL)]. Cells were stimulated with CELLection Pan Mouse IgG (Invitrogen) coated with anti-CD3 (UCHT1), anti-CD28 (CD28.2), and PD-L1 chimeric protein (kindly provided by G.J. Freeman) or the appropriate isotype control (IgG2a). Beads were added to CD4+ T cells in a 1:2 (cell:bead) ratio and kept in culture for the entire duration of the experiment (up to 9 days).
Fifteen to 25 × 106 CD8-depleted PBMCs or CD56- and CD8-depleted PBMCs from virally suppressed individuals were stimulated with SEA/SEB (0.3 ng/mL), IL-7 (10 ng/mL), or bryostatin (10 nM, 18 h exposure) in the presence or absence of anti-PD-1 (pembrolizumab 10 µg/mL) or the appropriate isotype control, in the presence of ARVs. Viral production was measured in cell culture supernatants after 3 or 6 days of culture as described above. Samples with spontaneous viral production (>10 HIV RNA copies in the nonstimulated condition) and/or inconsistent replicate values attributed to low frequency of reservoir cells were excluded. Cytokine production was measured by electrochemiluminescence using multi-array technology (Meso Scale Discovery). Bcl-2, CD69, Ki67, CD25, and HLA-DR/CD38 expressions, phosphorylation of STAT5 and Akt and cell viability were measured by flow cytometry using the following antibodies: Bcl-2-V450 (clone Bcl-2/100, BD#560637), CD69-APC (clone FN50, BD#555533), CD25-BUV737 (clone 2A3, BD#564385), HLA-DR-BUV395 (clone G46-6, BD#564040), CD38-PE (clone HIT2, BD#555460), pSTAT5-AF647 (pY694, BD#612599), and pAkt-PE-CF594 (pS473, BD#562465). PD-1 receptor occupancy by the blocking antibody was measured in all experiments by flow cytometry and was constantly higher than 90%.
Phosphorylation of CDK9 (Ser175) (provided by Merck) and level of expression of CyclinT1 were measured by flow cytometry after 12 h stimulation of CD4+ T cells isolated from virally suppressed individuals upon PD-1 engagement25. Briefly, titrated antibodies (CD3-FITC, CD4-AF700, CD8-PB, CD45RA-APC-H7, PD-1-BUV737) were added to 2 million cells in 50 μl of PBS in the presence of Live/Dead Dye for 30 min at 4 °C. Cells were fixed with Formaldehyde 4% (methanol free) for 20 min at room temperature and then permeabilized with Perm/Wash Buffer (BD#554723) for 30 min at 4 °C. Intracellular stainings for pCDK9 (pCDK9-AF647) and CyclinT1 (CyclinT1-TRITC, SantaCruz#8127) were conducted in Perm/Wash Buffer for 20 min at room temperature.
Memory CD4+ T cells were isolated by negative magnetic selection (StemCell) from fresh PBMCs obtained from HIV-negative individuals. After 24 h of TCR stimulation (0.1 µg/mL coated anti-CD3 and 1 µg/mL soluble anti-CD28), cells were nucleofected with pBlue3′LTR-lucB (NIH AIDS Reagent Program) using Amaxa technology (Lonza). Twenty-four hour postnucleofection, cells were stimulated with CD3/CD28 ± PD-L1 beads, as described above. Luciferase activity was measured after 6 h of stimulation.
We performed Pearson’s correlation, t test and Mann–Whitney U tests with Prism 6.0 software. We considered two-sided p values of less than 0.05 significant.
Further information on experimental design is available in the Nature Research Reporting Summary linked to this article.
The data that support the findings of this study are available from the corresponding author upon reasonable request. Source data underlying all figures are provided as a Source Data file.
Finzi, D. et al. Identification of a reservoir for HIV-1 in patients on highly active antiretroviral therapy. Science 278, 1295–1300 (1997).
Wong, J. K. et al. Recovery of replication-competent HIV despite prolonged suppression of plasma viremia. Science 278, 1291–1295 (1997).
Chun, T. W. et al. Presence of an inducible HIV-1 latent reservoir during highly active antiretroviral therapy. Proc. Natl Acad. Sci. USA 94, 13193–13197 (1997).
Cameron, P. U. et al. Establishment of HIV-1 latency in resting CD4+ T cells depends on chemokine-induced changes in the actin cytoskeleton. Proc. Natl Acad. Sci. USA 107, 16934–16939 (2010).
Siliciano, R. F. & Greene, W. C. HIV latency. Cold Spring Harb. Perspect. Med. 1, a007096 (2011).
Procopio, F. A. et al. A novel assay to measure the magnitude of the inducible viral reservoir in HIV-infected Individuals. EBioMedicine 2, 872–881 (2015).
Siliciano, J. D. et al. Long-term follow-up studies confirm the stability of the latent reservoir for HIV-1 in resting CD4+ T cells. Nat. Med. 9, 727–728 (2003).
Day, C. L. et al. PD-1 expression on HIV-specific T cells is associated with T-cell exhaustion and disease progression. Nature 443, 350–354 (2006).
Trautmann, L. et al. Upregulation of PD-1 expression on HIV-specific CD8+ T cells leads to reversible immune dysfunction. Nat. Med. 12, 1198–1202 (2006).
Petrovas, C. et al. PD-1 is a regulator of virus-specific CD8+ T cell survival in HIV infection. J. Exp. Med. 203, 2281–2292 (2006).
Porichis, F. et al. Responsiveness of HIV-specific CD4 T cells to PD-1 blockade. Blood 118, 965–974 (2011).
Porichis, F. et al. High-throughput detection of miRNAs and gene-specific mRNA at the single-cell level by flow cytometry. Nat. Commun. 5, 5641 (2014).
Velu, V. et al. Enhancing SIV-specific immunity in vivo by PD-1 blockade. Nature 458, 206–210 (2009).
Mylvaganam, G. H. et al. Combination anti-PD-1 and antiretroviral therapy provides therapeutic benefit against SIV. JCI Insight 3, e122940 (2018).
Chomont, N. et al. HIV reservoir size and persistence are driven by T cell survival and homeostatic proliferation. Nat. Med. 15, 893–900 (2009).
Fromentin, R. et al. CD4+ T cells expressing PD-1, TIGIT and LAG-3 contribute to HIV persistence during ART. PLoS Pathog. 12, e1005761 (2016).
Chew, G. M. et al. TIGIT marks exhausted T cells, correlates with disease progression, and serves as a target for immune restoration in HIV and SIV infection. PLoS Pathog. 12, e1005349 (2016).
Perreau, M. et al. Follicular helper T cells serve as the major CD4 T cell compartment for HIV-1 infection, replication, and production. J. Exp. Med. 210, 143–156 (2013).
Banga, R. et al. PD-1(+) and follicular helper T cells are responsible for persistent HIV-1 transcription in treated aviremic individuals. Nat. Med. 22, 754–761 (2016).
Noto, A. et al. CD32(+) and PD-1(+) lymph node CD4 T cells support persistent HIV-1 transcription in treated aviremic individuals. J. Virol. 92, e00901–18 (2018).
Hurst, J. et al. Immunological biomarkers predict HIV-1 viral rebound after treatment interruption. Nat. Commun. 6, 8495 (2015).
Evans, V. A. et al. Programmed cell death-1 contributes to the establishment and maintenance of HIV-1 latency. AIDS 32, 1491–1497 (2018).
Freeman, G. J. et al. Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation. J. Exp. Med. 192, 1027–1034 (2000).
Hatano, H. et al. Cell-based measures of viral persistence are associated with immune activation and programmed cell death protein 1 (PD-1)-expressing CD4+ T cells. J. Infect. Dis. 208, 50–56 (2013).
Mbonye, U. R. et al. Phosphorylation of CDK9 at Ser175 enhances HIV transcription and is a marker of activated P-TEFb in CD4(+) T lymphocytes. PLoS Pathog. 9, e1003338 (2013).
Deeks, S. G. et al. International AIDS Society global scientific strategy: towards an HIV cure 2016. Nat. Med. 22, 839–850 (2016).
Bullen, C. K., Laird, G. M., Durand, C. M., Siliciano, J. D. & Siliciano, R. F. New ex vivo approaches distinguish effective and ineffective single agents for reversing HIV-1 latency in vivo. Nat. Med. 20, 425–429 (2014).
Hui, E. et al. T cell costimulatory receptor CD28 is a primary target for PD-1-mediated inhibition. Science 355, 1428–1433 (2017).
Kamphorst, A. O. et al. Rescue of exhausted CD8 T cells by PD-1-targeted therapies is CD28-dependent. Science 355, 1423–1427 (2017).
Kim, Y. K., Mbonye, U., Hokello, J. & Karn, J. T-cell receptor signaling enhances transcriptional elongation from latent HIV proviruses by activating P-TEFb through an ERK-dependent pathway. J. Mol. Biol. 410, 896–916 (2011).
Patsoukis, N. et al. PD-1 alters T-cell metabolic reprogramming by inhibiting glycolysis and promoting lipolysis and fatty acid oxidation. Nat. Commun. 6, 6692 (2015).
Pauken, K. E. et al. Epigenetic stability of exhausted T cells limits durability of reinvigoration by PD-1 blockade. Science 354, 1160–1165 (2016).
Ghoneim, H. E. et al. De novo epigenetic programs inhibit PD-1 blockade-mediated T cell rejuvenation. Cell 170, 142–157 e119 (2017).
Wang, C. et al. In vitro characterization of the anti-PD-1 antibody nivolumab, BMS-936558, and in vivo toxicology in non-human primates. Cancer Immunol. Res 2, 846–856 (2014).
McGary, C. S. et al. CTLA-4(+)PD-1(−) Memory CD4(+) T cells critically contribute to viral persistence in antiretroviral therapy-suppressed, SIV-infected Rhesus macaques. Immunity 47, 776–788 e775 (2017).
Guihot, A. et al. Drastic decrease of the HIV reservoir in a patient treated with nivolumab for lung cancer. Ann. Oncol. 29, 517–518 (2018).
Wightman, F. et al. Effect of ipilimumab on the HIV reservoir in an HIV-infected individual with metastatic melanoma. AIDS 29, 504–506 (2015).
Scully, E. P. et al. Inconsistent HIV reservoir dynamics and immune responses following anti-PD-1 therapy in cancer patients with HIV infection. Ann. Oncol. 29, 2141–2142 (2018).
Ostios-Garcia, L. et al. Safety and efficacy of PD-1 inhibitors among HIV-positive patients with non-small-cell lung cancer. J. Thorac. Oncol. 13, 1037–1042 (2018).
Uldrick, T. S. et al. Interim safety analysis of cancer immunotherapy trials network-12 (CITN-12): A phase 1 study of pembrolizumab in patients with HIV and cancer. In Eighth International Workshop on HIV Persistence during Therapy (Journal of Virus Eradication, 2017;3 supplement 5).
Khoury, G. et al. Human immunodeficiency virus persistence and T-cell activation in blood, rectal, and lymph node tissue in human immunodeficiency virus-infected individuals receiving suppressive antiretroviral therapy. J. Infect. Dis. 215, 911–919 (2017).
Damouche, A. et al. High proportion of PD-1-expressing CD4(+) T cells in adipose tissue constitutes an immunomodulatory microenvironment that may support HIV persistence. Eur. J. Immunol. 47, 2113–2123 (2017).
Hatano, H. et al. Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers. AIDS 27, 2255–2260 (2013).
Vandergeeten, C. et al. Cross-clade ultrasensitive PCR-based assays to measure HIV persistence in large-cohort studies. J. Virol. 88, 12385–12396 (2014).
Vandergeeten, C. et al. Interleukin-7 promotes HIV persistence during antiretroviral therapy. Blood 121, 4321–4329 (2013).
Bounou, S., Leclerc, J. E. & Tremblay, M. J. Presence of host ICAM-1 in laboratory and clinical strains of human immunodeficiency virus type 1 increases virus infectivity and CD4(+)-T-cell depletion in human lymphoid tissue, a major site of replication in vivo. J. Virol. 76, 1004–1014 (2002).
The authors thank the participants who have generously donated samples for this study. The authors thank Moti Ramgopal, Brenda Jacobs, Mario Legault, Guillaume Thériault, Catalin Mihalcioiu, and Josée Girouard for recruitment and clinical assistance with study participants, Gordon Freeman for providing the PD-L1 chimeric protein, Sheri Dubey and Danilo Casimiro for cytokines quantifications, Jonathan Karn, Curtis Dobrowolski, Marion Pardons for assistance with P-TEFb measurements and Mariam Lawani, Wendy Picano, and Hawley Rigsby for technical assistance. We also thank the flow cores at UCSF, VGTI Florida (Yu Shi and Kim Kusser) and at the CRCHUM (Dominique Gauchat and Annie Gosselin) for cell sorting and the NC3 core at the CRCHUM (Olfa Debbeche). The authors thank Marta Massanella and the members of the Cleveland Immunopathogenesis Consortium for advice and helpful discussions. This work was supported the Delaney AIDS Research Enterprise (DARE) to Find a Cure 1U19AI096109 and UM1AI126611, NIH grant 1R21AI113096, by the Foundation for AIDS Research (amfAR Research Consortium on HIV Eradication 108687-54-RGRL and 108928-56-RGRL), by the Canadian Institutes for Health Research (#364408), by The Canadian HIV Cure Enterprise Team Grant (Cancure) from the CIHR in partnership with CANFAR and IAS (HIG-133050), and by the Réseau SIDA maladies infectieuses (FRQ-S). The SCOPE cohort was supported the UCSF/Gladstone Institute of Virology & Immunology CFAR (P30 AI027763) and the CFAR Network of Integrated Systems (R24 AI067039). Additional support was provided by the Delaney AIDS Research Enterprise (DARE; AI096109, A127966) and the amfAR Institute for HIV Cure Research (amfAR 109301). S.R.L. is supported by a practitioner fellowship from the National Health and Medical Research Council (NHMRC) of Australia. J.P.R. holds the Louis Lowenstein Chair in Hematology & Oncology in the Faculty of Medicine at McGill University. N.C. is supported by a Research Scholar Career Award of the Quebec Health Research Fund (FRQ-S, #30950).
D.J.H. is an employee and shareholder of Merck & Co., Inc., manufacturer of pembrolizumab. S.G.D. has received grant support from Gilead, Merck, and ViiV. He has consulted from AbbVie, Janssen and Shionogi. He is a member of the scientific advisory boards for Enochian Biosciences and BryoLogyx. N.C. has received grant support from Merck. The remaining authors declare no competing interests.
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Fromentin, R., DaFonseca, S., Costiniuk, C.T. et al. PD-1 blockade potentiates HIV latency reversal ex vivo in CD4+ T cells from ART-suppressed individuals. Nat Commun 10, 814 (2019). https://doi.org/10.1038/s41467-019-08798-7
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