The preparation of α-functionalized organic acids can be greatly simplified by adopting a protocol involving the catalytic assembly of achiral building blocks. However, the enzymatic assembly of small amino acids and aldehydes to form numerous α-functionalized organic acids is highly desired and remains a significant challenge. Herein, we report an artificially designed chiral-group-resetting biocatalytic process, which uses simple achiral glycine and aldehydes to synthesize stereodefined α-functionalized organic acids. This cascade biocatalysis comprises a basic module and three different extender modules and operates in a modular assembly manner. The engineered Escherichia coli catalysts, which contained different module(s), provide access to α-keto acids, α-hydroxy acids, and α-amino acids with excellent conversion and enantioselectivities. Therefore, this biocatalytic process provides an attractive strategy for the conversion of low-cost achiral starting materials to high-value α-functionalized organic acids.
The preparation of multifunctional chiral small molecules such as unnatural α-amino acids and α-hydroxy acids has attracted extensive attention from organic chemists because they play prominent roles in chemical synthesis, cosmetics, and pharmaceutical manufacturing1,2,3,4,5. Therefore, a few elegant chemical and enzymatic synthesis methods have been elaborated to provide a sufficient number of chemicals to meet market demand6,7,8,9. Among them, one particularly attractive synthesis route is the C–H functionalization-mediated asymmetric assembly of small building blocks10,11.
However, modern chemical C–H functionalization methods heavily rely on precious and unsustainable transition metals (e.g., Pd, Rh, Ru, and Ir) and protected substrates12,13,14. Hence, some researchers have turned to biocatalytic processes using some key enzymes such as threonine aldolases (TAs)9,15,16,17, which could catalyze the aldol assembly of unprotected small amino acids (principally glycine) and aldehydes to produce small organic acids, providing a dramatically simplified method. Another representative example is pyruvate aldolase, which uses alanine and formaldehyde as starting materials for the biosynthesis of (S)- and (R)-2-amino-4-hydroxybutanoic acid9. Unfortunately, these methods only provide a limited range of products such as β-hydroxy-α-amino acids and L/D-homoserine9,18,19. Therefore, the assembly of small amino acids and aldehydes to form numerous α-keto acids and α-hydroxy acids is highly desired and remains a significant challenge. Fortunately, artificially designed multienzyme cascades, which offer a alternative to related chemical methods and protein engineering3,11,17,20, provide a useful tool for overcoming some challenging reactions21,22,23,24,25,26, such as the oxy- and amino-functionalization of alkenes21,24,27,28.
Here, we report an artificially designed chiral-group-resetting cascade biocatalysis protocol, which could transform unprotected achiral building blocks into prochiral α-keto acids and then to chiral α-hydroxy acids and α-amino acids. The platform operates in a modular assembly manner, enabling the tunable and predictable operation of cascade biocatalysis and retaining a high product diversity (including aliphatic, aromatic, heteroaromatic, and heterocyclic products) and modularity.
Designing enzyme cascades for chiral group resetting
The designed modular cascade catalysis platform has two parts (Fig. 1a): (i) a basic module (BM), in which achiral aldehyde and glycine are assembled to prochiral α-keto acid (including the introduction and deletion of chiral –OH/–NH2 groups), and (ii) extender modules (EMs), in which α-keto acid is converted into α-hydroxy/amino acid by reintroducing chiral –OH/–NH2 groups (Fig. 1b). When a BM is coupled with EMs, for chiral –OH/–NH2 groups, the whole process is presented as introduction–deletion–reintroduction, which can be seen as an –OH/–NH2-group resetting process (Fig. 2a, b). Using easily available glycine as a functionalized primer and nine aldehydes as extender units (Fig. 1c), nine prochiral α-keto acids, 18 chiral α-hydroxy acids, and 18 chiral α-amino acids (Supplementary Fig. 1) were obtained.
The BM starts with the TA-mediated asymmetric assembly (C–C bond formation while introducing chiral –OH/–NH2 groups) of the achiral functionalized primer glycine (2) with aldehyde extender units (1) to produce β-hydroxy-α-amino acids (3) (Fig. 1b). The subsequent threonine deaminase (TD)-catalyzed atypical deamination (PLP-dependent α,β-elimination reaction29, Supplementary Fig. 2) led to the elimination of the chiral group (Cα-NH2 and Cβ-OH) of 3 while producing prochiral α-keto acids (4).
On the basis of the BM, EMs were designed for reintroducing chiral –OH/–NH2 groups (Fig. 1b). Extender module 1 (EM1) was designed for the S- or R-selective reduction of 4 to (S)- or (R)-hydroxy acids [(S)- or (R)-5] catalyzed by L- or D-hydroxyisocaproate dehydrogenase (ʟ- or D-HicDH), coupled with NADH recycling by formate dehydrogenase (FDH). Extender module 2 (EM2) employed ʟ-amino acid dehydrogenase (ʟ-AADH) for converting 4 into (S)-α-amino acids [(S)-6] and FDH for NADH recycling. Since D-AADHs are not ubiquitous in nature, as opposed to ʟ-selective AADHs, and natural D-AADHs are membrane-bound enzymes dependent on NADPH, which are much more expensive than NADH30, we chose D-amino acid transaminase (D-AATA) for extender module 3 (EM3) for transforming 4 into (R)-α-amino acids [(R)-6] and glutamate dehydrogenase (GluDH), FDH, and glutamate racemase (GLR) for amino donor recycling.
The enzymes used in all of these modules were selected on the basis of literature reports on the abilities of specific enzymes and organisms to function in the presence of the required intermediates. For the further assembly of multiple modules and the optimization of enzyme expression in one E. coli strain, every module was cloned into four different but compatible plasmids (Supplementary Table 1)—pACYCDuet-1, pCDFDuet-1, pETDuet-1, and pRSFDuet-1—to generate four recombinant plasmids (Supplementary Table 2)28. The protein expressions from different plasmids harbored within the cells were induced with isopropyl-β-D-1-thiogalactopyranoside (IPTG), and an SDS-PAGE analysis confirmed the soluble expression of all of the enzymes used.
Production of prochiral α-keto acid
The validation of this system was initially focused on building a capable BM (Fig. 1b); therefore, the talented enzymes TA and TD were needed. First, Pseudomonas aeruginosa TA (PaTA) was selected owing to its high activity toward 1a and 231,32. Then, for TD screening, eight functionally known TDs were selected from the NCBI database via data mining33 and expressed in E. coli BL21 (DE3). In order to obtain a complete conversion of 3 to 4, the TD activities toward both threo- and erythro-ʟ-phenylserine (threo/erythro-3a) were tested, and only TD from Corynebacterium glutamicum (CgTD) showed activities toward both configurations. Therefore, PaTA and CgTD were overexpressed and purified (Supplementary Fig. 3). To identify the potential bottleneck in the BM, the effects of the TA and TD concentrations on the initial reaction rate of 1a to 4a were examined in vitro. As shown in Fig. 3a, the initial reaction rate of 1a to 4a was slightly increased (7%) when increasing the TA concentration by twofold, while it was increased by 1.6-fold when doubling the concentration of CgTD. These results indicate that CgTD was the rate-limiting enzyme in the C–C bond formation and α,β-elimination cascade reaction. Furthermore, the activity of CgTD toward different substrates was investigated, and the results are shown in Fig. 3b. It was found that the activity of CgTD significantly decreases as the substrate size increased. In comparison with the case using 3i as a substrate (a natural substrate of CgTD), the relative activity of CgTD toward bulky substrate 1a decreased to 5% and exhibited no activity toward larger substrate 3e (Fig. 3b). Therefore, to meet the needs of α-keto acid synthesis, it is imperative to improve the substrate specificity of CgTD toward bulky substrates by protein engineering strategies.
Then, we built a structural model of CgTD via homologous modeling by using E. coli TD (PDB ID: 1TDJ) as the template. To simulate the orientation of the external aldimine intermediate34, ligands (3a and 3i) were created with the nitrogen atom of 3 linked to the PLP cofactor (Fig. 3c, d). Docking these ligands into the cofactor-free structure yielded outputs with the PLP and substrates with approximately the same orientation, suggesting that bulky substrates show the correct orientation. The surface of the binding pocket (Fig. 3e) also indicated that the volume of the catalytic cavity is sufficiently large to accept bulky substrates. On the other hand, by analyzing the substrate channel of CgTD, steric hindrance was identified as a potential bottleneck (a small entrance, Fig. 3f) for the bulky substrates. Two residues (Phe114 and Arg229) were identified as potential hot spots for broadening the substrate channel. Semisaturated mutation was used for protein engineering, and the highest initial reaction rate (667 µM min−1) was observed for 1a with the variant CgTDF114A,R229T (with a larger substrate channel, Fig. 3g), thus representing a greater-than-18-fold increase when compared with that of CgTDWT for 1a (37 µM min−1).
Four BM plasmids containing the PaTA and CgTDF114A,R229T genes were transformed into the host strain that led to E. coli (OA01-04) (Supplementary Table 3). Among them, E. coli OA02 was found to be the best whole-cell catalyst in the biotransformation of 1a into 4a (Supplementary Fig. 4), which produced 380 mg l−1 4a. Furthermore, the reaction conditions for the conversion of 1a to 4a were optimized, including the pH, temperature, E. coli (OA02) concentration, and cosolvents (Supplementary Fig. 5). It was found that under the optimum conditions (pH 8.0, 25 °C, 10 g dcw l−1, and 10% DMSO), the titer of 4a was improved to 1494 mg l−1 with a conversion of 91%. However, low levels of unreacted substrate 1a (2%) and intermediate 3a (4%) still remained in the conversion system.
The synthetic potential of the BM was then tested with a range of aldehydes (1b–i), affording the corresponding α-keto acids (4b–i) at good conversions (68–91%) (Fig. 4). Overall, the conversion is higher than that in the case of PaTA alone (conversion < 80%, d.e. < 55%)31, which indicated that the BM could overcome the thermodynamically unfavorable equilibria of the TA reaction by coupling it with the irreversible TD reaction. However, even with the BM cascade system, it still cannot lead to complete conversion, which is possibly due to the reversible nature of the TA reaction and the relatively low activity of CgTD for the second reaction. For cascade processes, it is important that the second step is much faster than the first step to reach the theoretical conversion of 100%28. Another reason is the metabolic background of E. coli, which could convert aldehydes into carboxylic acids or the corresponding alcohols and cause a loss of the starting material35.
Production of α-hydroxy acid by reintroducing chiral –OH
To efficiently reintroduce chiral –OH for converting keto acids into α-hydroxy acids, the highly selective ʟ- or D-HicDH, (ʟ-HicDH obtained from Lactobacillus confusus DSM 201966 and D-HicDH obtained from Lactobacillus paracasei DSM 20008)36,37 was chosen and used to construct EM1. Then, four BM plasmids and eight EM1 plasmids were combinatorially combined and transformed into E. coli to engineer the catalysts for the conversion of glycine and aldehydes into α-hydroxy acids. Since plasmids with the same backbone are not compatible with each other, 24 different E. coli strains (OA05-28) were obtained (Supplementary Table 3), each coexpressing PaTA, CgTD, CbFDH, and highly selective ʟ- or D-LDH (E. coli OA05-16 and E. coli OA17-28 provide S- and R-selective reducing activities, respectively). Next, the capacities of those 24 types of E. coli strains (OA05-28) on the transformation of 1a into (S)- and (R)-5a were examined, and the results showed that E. coli (OA15) and E. coli (OA23) are the best whole-cell catalysts, which produced 1470 mg l−1 of (S)-5a (>99% e.e. and 89% conversion; Supplementary Fig. 6) and 1404 mg l−1 of (R)-5a ( > 99% e.e. and 85% conversion, Supplementary Fig. 7), respectively, in 36 h. The intermediate 4a was found at relatively low levels (<1%); this indicated that EM1 has a high conversion efficiency.
To further explore the potential of the engineered cascade, aldehydes 1b–i (10 mM) were biotransformed into α-hydroxy acids by using E. coli (OA15) and E. coli (OA23). As summarized in Table 1, (S)- and (R)-5 were obtained with 55–92% conversion with excellent enantioselectivities (96–99%), demonstrating that the enzymatic cascade comprising BM and EM1 has a high efficiency and broad applicability.
Production of α-amino acid by reintroducing chiral –NH2
In order to reintroduce the chiral –NH2 group, ʟ-AADH from Bacillus badius38 was selected to construct EM2 for converting α-keto acids into (S)-α-amino acids. The combinatorial assembly of four BM plasmids and four EM2 plasmids led to 12 different E. coli whole-cell catalysts (OA29-40) (Supplementary Table 3), each coexpressing PaTA, CgTD, CbFDH, and highly selective ʟ-AADH. Consequently, the 12 constructed E. coli strains were used for the transformation of 1a into (S)-6a, and E. coli (OA34) showed the best conversion capacities, which produced 1478 mg l−1 (S)-6a (90% conversion and 95% e.e., Supplementary Fig. 16). Furthermore, E. coli (OA34) was used to catalyze different aldehydes 1b–i (Table 2) to the corresponding (S)-amino acids, and excellent conversions of 65–93% (>89% e.e.) were detected.
Similarly, in order to produce (R)-α-amino acids, 12 different E. coli strains (OA41-52) were obtained, each coexpressing PaTA, CgTD, glucose dehydrogenase from Bacillus megaterium (BmGDH, for NADPH recycling), and D-AADH (a meso-2,6-D-diaminopimerate dehydrogenase mutant from Symbiobacterium thermophilum39, StDAPDHH227V). However, the best strain of E. coli (OA51) produced only 370 mg l−1 (R)-6a (Supplementary Fig. 17). In order to improve the conversion of (R)-α-amino acids, we recruited the transamination route in EM3 (expressing B. subtilis D-AATA) (Fig. 1b) since D-AADHs are not ubiquitous in nature as opposed to ʟ-selective AADHs (only meso-DAPDHs have been reported)30. Thus, four BM plasmids and four EM3 plasmids were combinatorially assembled to obtain 12 E. coli strains (OA53-64) (Supplementary Table 3). The 12 obtained types of E. coli strains were used for the conversion of 1a into (R)-6a. Finally, the highest titer of (R)-6a (1396 mg l−1 with 85% conversion and 99% e.e., Supplementary Fig. 18) was obtained with E. coli (OA59). The potential of E. coli (OA59) was examined with aldehydes 1b–i (Table 2), and (R)-6 was produced with conversions of 71–89%, except (R)-6e (32%) and (R)-6g (64%). Remarkably, all of the produced (R)-α-amino acids 6a–i were in their enantiopure forms (e.e. >98%). In conclusion, the enzymatic cascade coupling BM with EM2/EM3 exhibited great potential for the synthesis of (S)/(R)-α-amino acids from renewable glycine and aldehydes.
Synthesis of α-functionalized organic acids on a 100-ml scale
The synthesis of α-functionalized organic acids was scaled up to 100 ml to further evaluate the feasibility of the biocatalytic cascades. First, the tolerance of the key enzyme CgTD to 1a and the ratio of the catalyst to substrate 1a were optimized for the scaled-up biotransformations, and the optimum conditions were identified to be 50 mM 1a with catalyst-to-substrate ratios of 0.5–1:1 (g dcw l−1 mM−1) at 25 °C, pH 8.0, and 5% DMSO (Supplementary Fig. 27). Then, the preparative biotransformations of 1a (50 mM) with E. coli (OA02), E. coli (OA23), and E. coli (OA59) (30 g dcw l−l) were performed. An additional 40-mM 1a was added after 6 h when the remaining concentration of 1a was lower than 10 mM. Subsequently, a total of 12.5 g l−1 4a, 12.1 g l−1 (R)-5a (99% e.e.), and 11.9 g l−1 (R)-6a (99% e.e.) was produced at 85, 81, and 80% conversion, respectively, in 30–42 h. Further, the space–time yields (STYs) of 4a, (R)-5a, and (R)-6a reached 10.0, 6.9, and 6.8 g l−1 d−1, respectively. Finally, 4a, (R)-5a, and (R)-6a were isolated at 75, 69, and 61% yield by chromatography, extraction, and crystallization. Similarly, seven other valuable chemicals (4i, (S)-5e, (S)-5i, (R)-5i, (S)-6e, (S)-6g, and (R)-6h) with many applications (Supplementary Table 4)) were successfully synthesized using the corresponding E. coli whole-cell catalysts and were isolated in yields of 45–78%.
In this study, we report an artificially designed biocatalytic cascade, which comprises a BM and three different EMs, for the C–H functionalization-mediated asymmetric synthesis of α-functionalized organic acids. This biocatalytic cascade represents a chiral-group-resetting process that operates in a modular assembly manner, enabling the change of different catalyst modules and substrate units to facilitate the control of product species and configurations. Using this biocatalytic process with glycine as a primer and different aldehydes as extender units, 45 types of aliphatic, aromatic, heteroaromatic and heterocyclic products were obtained, including nine prochiral α-keto acids, 18 chiral α-hydroxy acids, and 18 chiral α-amino acids. Therefore, this biocatalytic process exhibited great potential for the production of a variety of α-functionalized organic acids.
The chiral group resetting process reported here provides a useful tool for the regulation of product configurations and species and helps to expand the application of unsatisfactory enzymes (such as TAs). During this process, the chiral information of each product was changed via three steps (Fig. 2): (i) introduce chiral –OH/–NH2 groups (low selectivity), (ii) delete chiral –OH/–NH2 groups, and (iii) reintroduce –OH/–NH2 groups (with the desired configurations and species). With this process, both optically pure α-amino acids and α-hydroxy acids were finally obtained. Furthermore, this artificially designed biocatalytic process also overcame the thermodynamic (reversible reaction) and kinetic limitations (low diastereoselectivity for β-carbon) associated with the use of TAs31,40,41 by cascading with an irreversible TD reaction. From an organic synthesis perspective, for the regulation of product configurations and species, the chiral-group-resetting process is unique and advantageous over the majority of other enzymatic processes, e.g., asymmetric reduction (from achiral to S/R-NH2/-OH)3,39, stereoinversion (from S-NH2/-OH to R-NH2/-OH)3,4,5, deracemization (from rac-NH2/-OH to R-NH2/-OH)3,4,5, and type conversion (from S-NH2 to S/R-OH)26,36,37. In related chemical methods, the product configurations and species are the same as the substrate since the active chiral functional groups (e.g., –OH and –NH2)10,11 were protected.
The achiral glycine and aldehydes, which are readily available and inexpensive, for the production of α-hydroxy acids and α-amino acids represent a sustainable alternative to other complementary methods. In contrast, in most enzymatic production processes of α-hydroxy acids and α-amino acids, the commonly used substrates are keto acids3,39, (S)-amino acids or their racemic mixtures3,26,36, and (S)-hydroxy acids or their racemic mixtures4,5; these are not readily available and much more expensive than the corresponding aldehydes. Regarding chemical methods, the protection and deprotection processes have the particular drawback of cost due to the protected substrates that are needed, such as N-phthalimido-protected L-α-alanine11 and methyl or benzyl-protected (S)-α-lactic acid10. Thus, this biocatalytic process provides an attractive strategy for the conversion of low-cost achiral starting materials to high-value α-functionalized organic acids.
The biocatalytic process in this study is elegant and nicely exploits the power of cascades: (i) it provides a universal, efficient, and renewable catalyst to replace precious transition metals such as Pd10,11 in the modern chemical C–H functionalization-mediated asymmetric assembly of α-functionalized organic acids and (ii) when compared to other enzymatic methods3,4,5,9,26,28,36,39, the universality and practicality of this biocatalytic cascade is more attractive owing to the more diverse product range that it supports, including liphatic, aromatic, heteroaromatic, and heterocyclic products from three classes (α-keto/hydroxy/amino acids). Furthermore, since enantiocomplementary ʟ- and D-specific TAs were identified to accept nucleophilic substrates other than glycine (such as alanine, serine, and cysteine)42,43, the expansion of this biocatalytic process could enable the synthesis of a vast array of α-functionalized products simply by combinatorically integrating different components such as other catalyst modules and substrate units.
Strains and plasmids
Wild-type E. coli BL21 DE3 was used for all molecular cloning and biocatalysis experiments. Plasmid based gene overexpression was achieved by cloning the desired gene(s) into a set of compatible plasmids pACYCDuet-1, pCDFDuet-1, pETDuet-1, and pRSFDuet-1 (Novagen, Darmstadt, Germany) (Supplementary Table 1) digested with appropriate restriction enzymes (see Supplementary Methods for genetic constructions). Further transformation of constructed plamid(s) into E. coli strain gave an E. coli strain containing one or two designed modules for the desired transformations. All resulting plasmids and strains used in this study are listed in Supplementary Tables 2 and 3.
The structure model of CgTD and its variants were built via SWISS-MODEL [https://swissmodel.expasy.org/] by using E. coli TD (PDB ID: 1TDJ [https://www.rcsb.org/structure/1TDJ]) as template. Docking simulations were performed using Autodock Vina and CgTD models. BioLuminate (Schrödinger) was used to draw ligands, and AutoDockTools was used to generate PDBQT files. To simulate the orientation of the external aldimine intermediate, ligands were created having the nitrogen atom linked to the PLP cofactor.
Site-directed mutagenesis was performed by PCR using mutagenic primers and plasmid pETduet-CgTD as template according to the manufacturer’s instructions of QuickChange (Stratagene) (see Supplementary Methods for directed evolution experiments). The primers used in this study are listed in Supplementary Table 5. The DpnI-digested PCR product of 3 μl was used to transform 80 μl of E. coli JM109 chemically competent cells and colonies after transformation were incubated for DNA sequencing until all the designed mutants were obtained. Subsequently the plasmid of each mutant was extracted and transformed into E. coli BL21(DE3) for enzyme expression.
Analytic transformation of 1a–i (10 mM) to 4a–i were conducted with resting cells of E. coli (OA02) (10 g dcw l−1) in 2 ml KP buffer (50 mM, pH 8.0, 100 µM PLP, and 10% DMSO) at 200 rpm and 25 °C for 24 h. Analytic transformation of 1a–i (10 mM) to (S)- and (R)-5a–i were conducted with resting cells of E. coli (OA15) and E. coli (OA23) (10 g dcw l−1) in 2 ml KP buffer (50 mM, pH 8.0, 100 µM PLP, and 10% DMSO) at 200 rpm and 25 °C for 36 h, respectively. Analytic transformation of 1a–i (10 mM) to (S)- and (R)-6a–i were conducted with and resting cells of E. coli (OA33) and E. coli (OA71) (10 g dcw l-1) in 2 ml KP buffer (50 mM, pH 8.0, 100 µM PLP, and 10% DMSO) at 200 rpm and 25 °C for 36 h, respectively. At the end of the reaction, 100 μl of supernatant was separated after centrifugation (13000 g, 2 min), diluted with MeCN/H2O solution (900 μl, 5:4) containing 0.1% TFA. The protein was removed by centrifugation and the solution was filtered through 0.22 µm membrane filters. The resulting solution was analyzed by HPLC for quantifying the products under the conditions stated below. To determine the e.e. of 5a–i, the sample (200 μl) at the end of reaction was separated after centrifugation (13,000 g, 2 min), acidified with HCl (2.0 M) and extracted with EtOAc. After evaporation of ethyl acetate, the residue was dissolved in solvent (n-hexane: IPA = 95:5) for chiral HPLC analysis (see Supplementary Methods for analysis methods).
Preparative transformation of 1a or 1i (50 mM initial concentration and 40 mM addition at 6 h; total 90 mM) to 4a or 4i was conducted with resting cells of E. coli (OA02) (30 g dcw l-1) in 100 ml KP buffer (50 mM, pH 8.0, 100 µM PLP, and 5% DMSO) at 200 rpm and 25 °C for 30 h. Preparative transformation of 1a, 1e, or 1i (50 mM initial concentration and 40 mM addition at 6 h; total 90 mM) to (S)-5e, (S)-5i, or (R)-5a was conducted with resting cells of E. coli (OA15) or E. coli (OA23) (30 g dcw l-1) in 100 ml KP buffer (50 mM, pH 8.0, 100 µM PLP, and 5% DMSO) at 200 rpm and 25 °C for 42 h, respectively. Preparative transformation of 1a, 1e, 1 g, or 1 h (50 mM initial concentration and 40 mM addition at 6 h; total 90 mM) to (S)-6e, (S)-6g, (R)-6a, or (R)-6h was conducted with and resting cells of E. coli (OA33) or E. coli (OA71) (30 g dcw l-1) in 100 ml KP buffer (50 mM, pH 8.0, 100 µM PLP, and 5% DMSO) at 200 rpm and 25 °C for 42 h, respectively. At the end of the reaction, the reaction mixtures were centrifuged at 6000 × g for 30 min, and the supernatants was collected for product isolation (see Supplementary Methods for isolation protocols). The isolated products were further identified by nuclear magnetic resonance (NMR) and high resolution mass spectrometry (HRMS) analysis (see Supplementary Figs 28–47 and Supplementary Notes).
The data supporting the findings of this study are available within the paper and its Supplementary Information files, or are available from the authors upon request.
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This work is supported by the National First-class Discipline Program of Light Industry Technology and Engineering (LITE2018-08), the Key Technologies R & D Program of Jiangsu Province (BE2018623), and the National Natural Science Foundation of China (21878126).