Autophagy emerges as an essential immunity defense against intracellular pathogens. Here we report that turnip mosaic virus (TuMV) infection activates autophagy in plants and that Beclin1 (ATG6), a core component of autophagy, inhibits virus replication. Beclin1 interacts with NIb, the RNA-dependent RNA polymerase (RdRp) of TuMV, via the highly conserved GDD motif and the interaction complex is targeted for autophagic degradation likely through the adaptor protein ATG8a. Beclin1-mediated NIb degradation is inhibited by autophagy inhibitors. Deficiency of Beclin1 or ATG8a enhances NIb accumulation and promotes viral infection and vice versa. These data suggest that Beclin1 may be a selective autophagy receptor. Overexpression of a Beclin1 truncation mutant that binds to NIb but lacks the ability to mediate NIb degradation also inhibits virus replication. The Beclin1–RdRp interaction further extends to several RNA viruses. Thus Beclin1 restricts viral infection through suppression and also likely autophagic degradation of the viral RdRp.
Macroautophagy (hereafter referred to as autophagy) is an evolutionarily conserved intracellular process in which double membrane-bound sack-like vesicles, termed autophagosomes, are biosynthesized to enclose and deliver cytoplasmic materials including macromolecular complexes and organelles to the lysosome/vacuole for degradation/recycling1,2,3,4,5. Autophagy may be divided into several major steps, including induction, nucleation and expansion of the phagophore, maturation of the autophagosomes, and docking/fusion with the vacuole or lysosome, followed by degradation and breakdown6,7,8. Extensive genetic screens of yeast mutants have led to the identification of ~40 autophagy-related genes (ATGs), and approximately half of them are indispensable for the formation of autophagosomes3, 5, 8. Most of these key ATGs in yeast have orthologs in mammals and plants1,2,5,6,9,10,11. During canonical autophagy, the corresponding gene products of the key ATGs form several functionally distinct groups to assemble the core autophagy machinery5,6,7,8. One of them is the phosphatidylinositol 3-kinase (PI3K) complex containing PI3K/VPS34, VPS15/p150, and Beclin1/ATG6/VPS30.
Initially, autophagy was assumed to be a rather unspecific, adaptive bulk catabolic process to provide energy and recycle nutrients for survival upon starvation stress12. It is now well established that autophagy can operate either in a non-selective or a highly selective mode, depending on the type of cargo to be degraded. Selective autophagy can target a variety of substrates ranging from individual proteins and protein complexes to entire organelles and invading microbes13,14. It involves the recruitment of specific receptor and adaptor proteins that simultaneously interact with specific cargoes and autophagy modifiers.
Autophagy is activated during infection by diverse viruses in metazoans15,16. Although autophagy has been suggested to play both antiviral and proviral roles, compelling evidence suggests that autophagy participates in innate and adaptive immune responses to eliminate pathogenic viruses17. For instance, in Drosophila, vesicular stomatitis virus infection induces autophagy, and depletion of ATGs by RNAi leads to increased virus replication and fly mortality18. In mice, ATG5 plays an essential role against lethal infection of the mouse central nervous system by Sindbis virus19. In plants, Liu and colleagues pioneered the research by showing that the autophagy pathway is required for N-gene mediated resistance to Tobacco mosaic virus (TMV)20. In contrast to the wild-type plants showing typical hypersensitive response (HR) upon TMV infection, plants deficient in the autophagy genes, Beclin1, PI3K/VPS34, ATG3, and ATG7, exhibit an unrestricted HR20. The suggestion of autophagy as an important component of plant immunity has been consolidated by two recent studies on the role of autophagy against two DNA viruses Cotton leaf curl Multan virus (CLCuMuV) and Cauliflower mosaic virus (CaMV)21,22,23. Hafréd and colleagues showed that autophagosomes are induced in CaMV-infected Arabidopsis, and the autophagy cargo receptor Neighbor of BRCA1 (NBR1) interacts with capsid proteins and mediates their degradation21. Consistently, Haxim el al. found that CLCuMuV infection induces autophagy in Nicotiana benthamiana22. In this case, the cargo acceptor is NbATG8f, an ATG8 family protein, which interacts with the satellite-encoded protein βC1, a major pathogenicity factor of CLCuMuV, and the interaction directs βC1 to autophagosomes for degradation22.
The majority of viruses infecting animals and plants are positive-sense RNA viruses. The molecular interactions between RNA viruses and plant autophagy still remain poorly understood. Potyviruses represent the largest group of known plant viruses with a positive-sense, single-stranded RNA as its genome and include many agriculturally important viruses such as Turnip mosaic virus (TuMV), Tobacco etch virus (TEV), Plum pox virus (PPV), and Soybean mosaic virus (SMV)24. A typical potyviral genome encodes a long open reading frame (ORF) and another relatively short ORF that results from RNA polymerase slippage in the P3 coding sequence. The resulting two polyproteins are proteolytically processed by three viral protease domains into 11 mature viral proteins, among which NIb is the only viral RNA-dependent RNA polymerase (RdRp), and HC-Pro and VPg are two known viral suppressors of RNA silencing (VSRs).
In this study, we show that TuMV infection activates autophagy and that Beclin1, one of the core ATGs that are upregulated by TuMV infection, interacts specifically with TuMV NIb, and targets NIb to autophagosomes for degradation likely via the key autophagic protein ATG8a. The interaction of NIb with Beclin1 also suppresses NIb RdRp activity in an autophagy-independent manner. Moreover, Beclin1 interacts with the RdRps of several other potyviruses as well as distinct RNA viruses via the GDD motif. These results suggest that Beclin1 restricts viral infection through suppression and also likely autophagic degradation of the viral RdRp.
TuMV infection activates autophagy
The autophagy pathway genes are constitutively expressed at the basal level to maintain homeostasis and are upregulated in response to abiotic and biotic stress13,14. To determine whether TuMV infection induces autophagy, we used yellow fluorescent protein (YFP)-tagged N. benthamiana ATG8a (YFP-NbATG8a) as an autophagosome marker to monitor autophagy25,26. Compared to mock-infiltrated N. benthamiana plants, the number of the punctate YFP fluorescent structures representing pre-autophagosomal or autophagosomal structures in TuMV-infected plants increased by more than two-folds (Fig. 1a, b and Supplementary Fig. 1a,b). A TuMV replication-defective mutant (TuMV-ΔGDD), in which the highly conserved GDD motif of NIb was deleted, was included in this assay. This deletion abolishes viral replication but still allows the production of viral proteins, expression of which is controlled by a 35S promoter. No significant changes were detected between the numbers of the punctate YFP-ATG8a fluorescent structures in mock- and TuMV-ΔGDD-inoculated plants (Fig. 1a, b and Supplementary Fig. 1a,b). These data indicate that TuMV infection activates autophagy.
We further examined the expression of ATG genes in these plants using quantitative real-time reverse-transcription PCR (qRT-PCR). The expression levels of all tested nine ATG genes, including NbBeclin1, NbPI3K, NbVPS15, NbATG2, NbATG9, NbATG3, NbATG5, NbATG7, and NbATG8a, were significantly upregulated in TuMV-inoculated leaves (Fig. 1c) or upper non-inoculated leaves (Fig. 1d), compared to their respective counterparts in mock- or TuMV-ΔGDD-inoculated plants. Transient expression of each of the 11 potyviral proteins alone under the control of 35S promoter failed to significantly upregulate these 9 ATG genes (Supplementary Fig. 2a, NIb used an example). As NIb is the only viral RdRp, we further confirmed that there were no significant differences in NbBeclin1 and NbATG8a induction and the amount of YFP-NbATG8a puncta at different NIb levels (Supplementary Fig. 2b–f). Consistently, high levels of Beclin1 and ATG8 proteins were detected only in TuMV-infected plants by immunoblotting (Fig. 1e, f). Transmission electron microscopy (TEM) was employed to further monitor autophagic activity induced by TuMV infection. In comparison with mock or TuMV-ΔGDD systemically infected leaves, TuMV infection induced an approximate eight-fold greater number of double-membrane structures typical of autophagosomes in the cytoplasm27 (Fig. 1g, h). Taken together, these results demonstrate that TuMV infection induces autophagy likely in a viral replication-dependent manner.
NbBeclin1 interacts with the viral RdRp
As NbBeclin1 (ATG6 in yeast or Arabidopsis) was the most upregulated gene (Fig. 1c, d) and its mammalian orthologous gene Beclin1 has been implicated in viral infection in mammalian cells28,29, possible interactions between NbBeclin1 and each of the 11 TuMV proteins30 were screened for by yeast two-hybrid (Y2H) assays. Positive protein–protein interaction was found between NbBeclin1 and TuMV NIb, and no interaction was evident between NbBeclin1 with any other TuMV proteins (Fig. 2a). The NbBeclin1–NIb interaction was confirmed by bimolecular fluorescence complementation (BiFC) in transgenic N. benthamiana leaves that express H2B-RFP, a nuclear marker (Fig. 2b). NIb-YFP was present in the cytoplasm and nucleus31,32,33. NbBeclin1-CFP was localized to a single or a few bright dots in the cytoplasm (Fig. 2c and Supplementary Fig. 3a), consistent with the distribution pattern of Arabidopsis AtATG6/VPS3034 (Supplementary Fig. 3a,b). When NIb-YFP and NbBeclin1-CFP were co-expressed, some NIb-YFP was re-distributed to the NbBeclin1-CFP-labeled punctate structures in the cytoplasm (Fig. 2c). Co-immunoprecipitation (Co-IP) assay showed that green fluorescent protein (GFP) antibody could specifically co-purify NbBeclin1-CFP and Myc-NIb (Fig. 2d), confirming the presence of the NbBeclin1-NIb complex in planta.
AtATG6/VPS30-labeled punctate structures are co-distributed with ATG8, an established autophagosome marker34. We thus tested the possible association of the NIb–NbBeclin1 complex with ATG8a. Co-expression and BiFC analyses showed that NbBeclin1-YFP and the NIb-NbBeclin1 complex indeed co-localized with NbATG8a-CFP to the punctate structure within the cytoplasm (Fig. 3a–c). These results indicate that the NbBeclin1-NIb complex is physically associated with autophagosomes.
Since NIb is an essential component of the viral replication complex (VRC), we hypothesized that NbBeclin1 is associated with the VRC through its interaction with NIb. We thus conducted a co-localization assay using NbBeclin1-YFP and a modified TuMV infectious clone TuMV-CFP-NIb35 (by insertion of cyan fluorescent protein (CFP) to the N-terminus of NIb in the infectious clone TuMV-6K2-mCherry36). The 6K2-induced structure derived from viral expression is an established marker of the VRC35. NbBeclin1-YFP co-localized with the 6K2-induced, CFP-NIb-containing irregular structures in the cytoplasm in TuMV-infected leaf cells (Fig. 4a). A dsRNA reporter35 that recognizes dsRNA such as replicative RNA intermediates also bound to the NbBeclin1-interacting, 6K2-induced structures, further supporting that they were active VRCs (Fig. 4b). To test whether the NbBeclin1-bound VRC interacts with autophagosomes, NbATG8a-YFP or NbATG8a-CFP was transiently co-expressed with NbBeclin1-CFP or the pair of YN-NbBeclin1/YC-NIb or YC-NbBeclin1/YN-NIb in N. benthamiana infected by TuMV-6K2-mCherry. NbATG8a-YFP or NbATG8a-CFP was indeed associated with the 6K2-induced VRC, which co-localized with NbBeclin1 or the NbBeclin1–NIb interaction complex (Fig. 4c–e). It was worthy to note that there was no co-localization between 6K2 and NbATG8a or NbBeclin1 when they were co-expressed together in the absence of viral replication in N. benthamiana leaves (Supplementary Fig. 4a–c). These results indicate that NbBeclin1 co-localizes with the VRC by binding to NIb.
NbBeclin1 mediates NIb degradation likely via autophagy
As NbBeclin1 may serve as a receptor protein for the autophagic clearance of viral proteins, we investigated whether NbBeclin1 has a role in the degradation of NIb. Immunoblotting analysis revealed that the levels of NIb-YFP markedly decreased when co-expressed with Myc-NbBeclin1 (Fig. 5a). A similar reduction was also observed when Myc-NIb was co-expressed with NbBeclin1-CFP (Fig. 5b). No obvious change in NIb or NbBeclin1 mRNA was observed when they were expressed alone or together (Supplementary Fig. 5a,b). To determine whether NbBeclin1-mediated NIb degradation holds true in the context of viral infection, we co-infiltrated N. benthamiana leaves with the infectious clone TuMV-CFP-NIb and an empty vector (Vec) or TuMV-CFP-NIb and Myc-NbBeclin1. Immunoblotting analysis confirmed that the levels of CFP-NIb from viral expression markedly decreased when co-expressed with Myc-NbBeclin1 (Fig. 5c). In contrast, overexpression of NbBeclin1 did not affect 6K2-GFP accumulation from non-viral expression (Fig. 5e). It is well known that virus intercellular movement does not occur for potyviruses until 72 hpi36. We thus determined the viral RNA level in the primarily infected cells at 60 hpi by qRT-PCR. We found that overexpression of NbBeclin1 significantly inhibited viral RNA accumulation (Fig. 5d). These results suggest that NbBeclin1-mediated degradation of NIb and its complexes including VRCs inhibits viral replication.
Drug treatment with 3-methyladenine (3-MA) and E64d, two well-known autophagy inhibitors, was performed to test whether the autophagy pathway is responsible for NbBeclin1-mediated degradation of NIb. 3-MA treatment enhanced the accumulations of NIb-YFP or CFP-NIb derived from viral expression (Supplementary Fig. 5c,d). In 3-MA-treated plants, the TuMV RNA level also increased (Supplementary Fig. 5e). E64d treatment showed similar results (Supplementary Fig. 5f,g). To observe the effect of impaired autophagy flux on the accumulation of autophagic bodies and viral genomic RNA, we conducted another drug treatment with the specific vacuolar ATPase inhibitor concanamycin A (Con A). Con A treatment led to the accumulation of significantly more autophagic bodies associated with VRCs and of enhanced levels of TuMV RNA at 72 hpi, compared to DMSO treatment, supporting the antiviral role of autophagy in viral infection (Supplementary Fig. 6).
Next, we determined whether NbBeclin1-mediated degradation of NIb was affected by autophagy inhibitors or by knockdown of NbATG8. Regardless of co-expression with NbBeclin1 or not, the levels of NIb-YFP or viral expression-derived CFP-NIb increased in 3-MA- or E64d-treated plants (Fig. 5f, g and Supplementary Fig. 5c,d,f,g). A TRV-based virus-induced gene silencing (VIGS) vector was employed to silence NbATG8a and NbATG8f, and knockdown of NbATG8a rather than NbATG8f remarkably inhibited NbBeclin1-mediated degradation of NIb-YFP or CFP-NIb (Fig. 5h, i and Supplementary Fig. 7a–c). A GFP protein that is relatively resistant to the autophagy pathway was used as a control (Fig. 5a, b and Supplementary Fig. 5h–k).
Mapping of the interacting domains
The protein domains required for the interaction between NbBeclin1 and NIb were mapped by Y2H assays (Fig. 6a and Supplementary Fig. 8a–d). The C-terminal portion (containing the APG6 domain) of NbBeclin1 (NbBeclin1-C) and the RdRp domain of NIb (NIb-M) were identified to be responsible for the NbBeclin1 and NIb interaction (Fig. 6a and Supplementary Fig. 8a–d). This observation was consistent with results from BiFC assays in planta (Supplementary Fig. 8e). The NIb-interacting domain NbBeclin1-C was also essential for the punctate localization of NbBeclin1 (Supplementary Fig. 8f).
Since the conserved GDD motif is located in NIb-M, we tested the importance of this motif with respect to the NIb–NbBeclin1 interaction. Deletion of GDD abolished the interaction (Supplementary Fig. 8b,d,g). As NIb-M is highly conserved among potyviruses32, we investigated the potential interaction between NbBeclin1 and NIbs from other potyviruses, including PPV, SMV, and TEV. Positive interactions were detected by Y2H assays (Supplementary Fig. 8g); consistently, GDD deletion extirpated the interaction (Supplementary Fig. 8g).
For selective autophagy, receptor proteins require binding to adaptors to facilitate docking of autophagy substrates to the autophagosomes37. To test whether NbBeclin1 binds to the autophagy adaptors ATG8 family proteins, the possible interaction between NbBeclin1 and NbATG8a or NbATG8f was analyzed. Y2H, BiFC, and Co-IP experiments consistently showed that NbBeclin1 interacted strongly with NbATG8a but not NbATG8f (Fig. 6b, c and Supplementary Fig. 9). The NbATG8-interacting domain was mapped to the N-terminal region of NbBeclin1 (NbBeclin1-N) (Fig. 6b, c and Supplementary Fig. 9). A web-based analysis (http://repeat.biol.ucy.ac.cy/iLIR)38 identified a potential ATG8 interacting motif (AIM) in NbBeclin1-N. The NbBeclin1-N AIM mutant (NbBeclin1-NΔAIM) resulting from the replacement of EESFVVL with EESAVVA lost the ability to interact with NbATG8a (Fig. 6b, c and Supplementary Fig. 9). NbBeclin1ΔAIM was still able to form bright granules, interact with, and co-localize with NIb in the cytoplasm (Supplementary Fig. 10a–c), consistent with the observation that the C-domain of NbBeclin1 (NbBeclin1-C) was required for NIb interaction and its punctate localization (Fig. 6a and Supplementary Fig. 8e,f). Autophagic structures were analyzed by TEM in N. benthamiana leaves transiently expressing NbBeclin1, NbBeclin1ΔAIM, NbBeclin1-N, NbBeclin1-NΔAIM, or NbBeclin1-C. Compared to buffer treatment (mock) and the expression of NbBeclin1ΔAIM, NbBeclin1-NΔAIM, or NbBeclin1-C, the expression of NbBeclin1 or NbBeclin1-N induced the formation of the typical double-membraned autophagosomes (Fig. 6d–f). Further, an autophagic flux assay on NbBeclin1 and its truncated mutants was conducted. Treatment of YFP:NbATG8a-expressing N. benthamiana leaves with Con A increased the number of NbATG8a-labeled puncta in the presence of NbBeclin1 or NbBeclin1-N but not other NbBeclin1 mutants (including NbBeclin1ΔAIM, NbBeclin1-NΔAIM, or NbBeclin1-C) (Supplementary Fig. 11a,b), indicating that NbBeclin1-N and its AIM was required to induce and enhance autophagy flux. These observations were also confirmed using western blot analyses (Supplementary Fig. 11c). The expression of NbBeclin1 or NbBeclin1-N, rather than NbBeclin1ΔAIM, NbBeclin1-NΔAIM, or NbBeclin1-C, decreased the levels of YFP-NbATG8a protein (Supplementary Fig. 11c). Treatment of these samples with E64d, an inhibitor of vacuolar cysteine proteases, blocked NbBeclin1- or NbBeclin1-N-mediated YFP-NbATG8a degradation (Supplementary Fig. 11c). These data suggest that NbBeclin1 induces autophagy through interacting with the autophagy adaptor NbATG8a via AIM (located at the N-terminus) and serves as an autophagy receptor by interacting with cargo proteins via its C-terminus.
NbBeclin1 also suppresses NIb independent of autophagy
NbBeclin1 co-localized and interacted with NbATG8a and NIb to form puncta and induce autophagy flux in the cytoplasm (Fig. 7a, b), and mediated their degradation (Fig. 7b and Supplementary Fig. 11d). The AIM deletion mutant NbBeclin1ΔAIM lost this capacity (Fig. 7a, b and Supplementary Fig. 11d). Western blots showed that NbBeclin1-mediated degradation of NbATG8a and NIb was obviously suppressed by the treatment of E64d or Con A (Supplementary Fig. 11d). These data support that NbBeclin1, as an autophagy cargo receptor to form autophagosomes and mediate cargo protein degradation, requires the AIM. Full-length NbBeclin1 (NbBeclin1-FL) and its truncated proteins (NbBeclin1-N and NbBeclin1-C) were compared for their ability to mediate NIb degradation. Both NbBeclin1-N and NbBeclin1-C lost this ability (Fig. 7d and Supplementary Fig. 11d), suggesting that NbBeclin1-N (which binds to NbATG8a via AIM to trigger autophagy) and NbBeclin1-C (which interacts with NIb) are both required for NbBeclin1-mediated NIb degradation. Consistent with the requirement of GDD for the NIb-NbBeclin1 interaction, NbBeclin1-FL failed to degrade NIb-ΔGDD-YFP (Fig. 7d). The effect of NbBeclin1, NbBeclin1-N and NbBeclin1-C on TuMV replication was analyzed. It was surprising that NbBeclin1-C failed to degrade NIb protein, but it was still able to suppress viral RNA replication (Fig. 7e). The expression of NbBeclin1 and NbBeclin1-C decreased viral RNA replication to approximately 8% and 25%, respectively (Fig. 7e). The fact that NbBeclin1-C is responsible for the NbBeclin1 and NIb interaction (Fig. 6a and Supplementary Fig. 8a–e) prompted us to consider the possibility that the interaction between NbBeclin1 and NIb may also interrupt viral replication independent of autophagy-mediated NIb degradation. Serial deletions of NbBeclin1-C were created and tested for the interaction with NIb by Y2H assays. As shown in Fig. 7f, the NIb-binding motif was mapped to the N-terminal region (C1) of NbBeclin1-C (strong interaction) or the N-terminal half (C4) of C1 (weak interaction). As expected, both C1 and C4 were unable to mediate NIb degradation (Fig. 7g). Overexpression of C1 or C4 rather than other NbBeclin1-C fragments inhibited viral replication (Fig. 7e), suggesting that NbBeclin1 binding to the RdRp domain of NIb suppresses RdRp activity in an autophagy-independent manner.
Beclin1 and ATG8a negatively regulate TuMV infection
Given that NbBeclin1-mediated NIb degradation requires NbATG8a, and NbBeclin1 inhibits RdRp activity by binding to the GDD motif, we next examined the effect of silencing NbBeclin1 or NbATG8a on viral replication. N. benthamiana plants were pre-inoculated with TRV-NbBeclin1 (to silence NbBeclin1), TRV-NbATG8a (to silence NbATG8a), or TRV-GUS (as a control) for 7 days and then agroinfiltrated with TuMV-GFP. GFP fluorescence in the inoculated leaves (at 3 dpi) and GFP progression in the upper non-inoculated leaves (at 6 and 30 dpi) were clearly enhanced in the NbBeclin1- or NbATG8a-silenced plants, compared to controls (Fig. 8a and Supplementary Fig. 12). In concordance, higher levels of TuMV genomic RNA were found in the NbBeclin1- or NbATG8a-silenced plants compared to controls (Fig. 8b). Protoplast transfection assays obtained similar results (Fig. 8c). In contrast, silencing of NbATG8f did not significantly affect TuMV infection (Supplementary Fig. 13). Overall, these results suggest that NbATG8a, and not NbATG8f, is required for NbBeclin1-mediated anti-TuMV defense.
To test whether this antiviral mechanism operates in another plant species, AtATG6, the ortholog of NbBeclin1 in Arabidopsis, was cloned. Y2H and BiFC analyses showed strong interactions between NIb and AtATG6 (Supplementary Fig. 14a,b). We obtained an atg6 heterozygous mutant (SALK_109281) from the Arabidopsis Biological Resource Center (ABRC) and generated AtATG6 overexpression transgenic Arabidopsis plants (35S:Myc-AtATG6) (Supplementary Fig. 15) for a TuMV infection assay. Compared to TuMV-infected wild-type Col-0, which showed typical symptoms as described39,40, the symptoms in 35S:AtATG6 were delayed for 3–4 days and were attenuated with mild chlorosis and slight stunting (Supplementary Fig. 14c). In contrast, atg6 plants showed more severe symptoms, including leaf necrosis and severe stunting (Supplementary Fig. 14c). TuMV RNA levels were significantly reduced in the 35S:AtATG6 plants but elevated in the atg6 mutants (Supplementary Fig. 14d). Similar results were obtained from the protoplast transfection assay (Supplementary Fig. 14e). We also obtained an Arabidopsis atg8a knockout mutant from ABRC and generated AtATG8a overexpression lines. Consistently, we found that knockout of AtATG8a enhanced viral RNA replication and symptom development, while overexpression of AtATG8a supressed viral infection (Supplementary Fig. 16).
NIb degradation mediated by Beclin1 requires ATG2
To further examine whether other key autophagy pathway genes participate in suppression of viral infection, we obtained Arabidopsis knockdown mutants of genes PI3K and VPS15 and Arabidopsis knockout mutants of genes ATG2, ATG5, and ATG7 and also knocked down these genes in N. benthamiana plants using the TRV vector. When PI3K or VPS15 was knocked down, the Arabidopsis or N. benthamiana plants became more susceptible (Supplementary Fig. 17), consistent with the results from Beclin1-deficient plants (Supplementary Fig. 14c,d). Knockout of AtATG2 in Arabidopsis enhanced TuMV RNA accumulation (Supplementary Fig. 18). In contrast, knockout of AtATG5 and AtATG7 did not affect TuMV infection (Supplementary Fig. 18). Similar results were observed when these genes were silenced in N. benthamiana (Supplementary Fig. 13). Western blot analyses revealed that Beclin1-mediated degradation of NIb was compromised in ATG2-deficient plants but not in ATG5- or ATG7-deficient plants (Supplementary Fig. 7). These data suggest that Beclin1-mediated NIb degradation requires ATG2 but independent of ATG5 and ATG7. Overexpression of NbBeclin1 in these ATG-deficient N. benthamiana plants (including NbATG8a-, NbATG8f-, NbATG2-, NbATG5-, or NbATG7-silenced plants) inhibited TuMV replication (Supplementary Fig. 7d), supporting that NbBeclin1 inhibits viral infection not only through NIb degradation (likely via ATG2/NbATGa-dependent autophagy) but also through degradation-independent suppression mechanisms.
NbBeclin1 targets other viral RdRps to restrict RNA viruses
The conserved GDD motif is present in almost all RdRps from diverse plant and animal viruses. In addition to TuMV NIb (Fig. 2), NbBeclin1 also interacted with NIbs from tested potyviruses including PPV, SMV, and TEV but not with their corresponding GDD deletion mutants (Supplementary Fig. 8g). To address whether NbBeclin1 also targets viral RdRps of viruses other than potyviruses, we cloned RdRps from Cucumber green mottle mosaic virus (CGMMV) of the Tobamovirus genus, and Pepino mosaic virus (PepMV) of the Potexvirus genus (Fig. 9a, b). NbBeclin1 strongly interacted with both RdRps (Fig. 9c). In both cases, the interacting domain was mapped to the GDD-containing RdRp2 domain and deletion of the GDD motif abolished the interaction (Fig. 9c). Co-IP and BiFC confirmed the data obtained from the Y2H assay (Fig. 9d, e).
The TRV VIGS vector was used to silence NbBeclin1- and the NbBeclin1-silenced plants were challenged with CGMMV and PepMV. The increased viral RNA levels of CGMMV or PepMV were detected in the inoculated leaves at 3 dpi and the upper systemically infected leaves at 14 dpi (Fig. 9f). Consistently, the NbBeclin1-silenced plants showed enhanced susceptibility to CGMMV and PepMV with more severe symptoms (Fig. 9g, h).
Successful viral infection results from molecular interplays between the invading virus and the host41,42,43,44. Viral infection activates autophagy, as shown during infection by many animal viruses15,16,17,18,19,45,46, and activated autophagy selectively degrades viral proteins as an important part of host immunity. A similar picture is also emerging in plants. As mentioned earlier, two recent publications have provided strong evidence that two ATGs, i.e., NBR1 and ATG8 can function as cargo receptor proteins to selectively interact with viral proteins to mediate their degradation and defend infection from two DNA viruses21,22. In this study, we found that infection by TuMV, a plant RNA virus, also significantly upregulated the autophagy pathway in plants (Fig. 1 and Supplementary Fig. 1). A TuMV RNA replication mutant, TuMV-ΔGDD, which could express all TuMV proteins including the replication-defective NIb, failed to activate autophagy and induce the high expression of ATGs (Fig. 1). Transient overexpression of individual potyviral proteins alone, including the wild-type NIb, failed to induce autophagy (Supplementary Fig. 2). These data lead us to suggest that autophagy induction is likely triggered by the viral replication process or viral RNA accumulation rather than individual potyviral proteins. We further found that Beclin1 interacted exclusively with NIb to mediate NIb degradation likely via the autophagy pathway (Figs. 2–5 and 7 and Supplementary Figs. 5–7, 11). Silencing of Beclin1 enhanced viral replication and symptom development, whereas overexpression of Beclin1 suppressed viral infection (Fig. 8). These data raise a possibility that Beclin1 is a selective receptor against TuMV infection. As a core component of the class III PI3K complex, Beclin1 may regulate autophagy by serving as a scaffold or interaction hub for interacting with diverse protein partners47. Genetic lesion studies demonstrate that Beclin1/ATG6 is a conserved requirement in autophagy in plants, humans, and other eukaryotes48. Consistent with this study, in their seminal work, Liu and colleagues reported that silencing of Beclin1 and several other ATGs enhances infection by TMV and autophagy is required for the timely HR to restrict TMV infection20. It is possible that Beclin1-mediated autophagy inhibits TMV infection via the interaction of Beclin1 and TMV RdRp, and autophagy regulates HR by a different mechanism. In mammalian cells, several viral proteins interact with Beclin1, which has been suggested to be an autophagy counteracting mechanism to promote viral infection49,50,51,52. It is not clear if any autophagy-mediated degradation also occurs on these Beclin1-interacting viral proteins.
Viral RdRp is absolutely required for positive-sense RNA virus replication and all viral RdRps have a conserved GDD motif53. We found that Beclin1 interacted with the RdRp domain of RdRps from all six test RNA viruses and the highly conserved motif GDD was essential for the interaction (Figs. 2, 6, and 9; Supplementary Fig. 8g). Overexpression of the partial NbBeclin1-C fragments (C1 or C4), losing the ability to mediate autophagy-dependent NIb degradation but competent for the interaction with GDD, inhibited TuMV replication (Fig. 7e), suggesting that Beclin1 binding to viral RdRp not only mediates its degradation but also suppresses its replication activity independent of Beclin1-mediated degradation.
ATG8 is a ubiquitin-like protein54 central to the autophagy pathway by binding to numerous cargo receptors and decorating autophagosomes5. In this study, we found that Beclin1 interacted with ATG8a but not ATG8f, Beclin1-mediated NIb degradation required ATG8a (Figs. 3–5; Supplementary Figs. 7 and 9), and the Beclin1-NIb complex or the Beclin1-NIb-VRC complex were co-localized with autophagosomes (Figs. 3 and 4). Beclin1 interacted with ATG8a using Beclin1’s AIM motif at the N terminus (Fig. 6b, c and Supplementary Fig. 9) and with NIb at its C-terminal region (Fig. 6a and Supplementary Fig. 8a–e). Thus it is very attempting to suggest that Beclin1 is a bridge to guide NIb to autophagosomes for degradation. Moreover, the AIM motif of Beclin1 was necessary for the formation of autophagosomes and induction of autophagy flux (Fig. 6d–f and Supplementary Figure 11a–c), supporting Beclin1 may play roles as an autophagy substrate and a functional receptor.
Silencing and overexpression of ATG8a affected plant susceptibility to infection in the same ways as Beclin1 (Supplementary Fig. 16). These findings revealed the biological importance of ATG8a in Beclin1-mediated selective autophagy in suppressing viral infection. However, knockdown of ATG8f did not significantly affect Beclin1-mediated NIb degradation and viral infection (Supplementary Figs 7 and 13). The fact that Beclin1 binds to NbATG8a rather than NbATG8f suggests that ATG8 family proteins may selectively bind to different autophagy receptors.
In addition to ATG8, we also examined the possible involvement of several other core autophagy proteins. Silencing or knockdown of PI3K or VPS15 promoted TuMV infection in N. benthamiana or Arabidopsis plants (Supplementary Fig. 17), which is not surprising as PI3K and VPS15, together with Beclin1, form the PI3K complex that initiates autophagy1,2,3,4,5. Knockout of another core autophagy gene ATG2, known to be involved in the early steps of autophagosome biogenesis, also increased plant susceptibility to TuMV infection (Supplementary Figs 13 and 18). In contrast, silencing of ATG5 or ATG7 did not affect viral infection (Supplementary Figs 13 and 18). We further found that it is not ATG5/ATG7 but ATG2 that is essential for the functionality of Beclin1-mediated degradation of NIb (Supplementary Fig. 7). The fact that NbBeclin1-mediated NIb degradation is dependent on ATG2 and independent of ATG5/ATG7 leads us to suggest that there is an alternative autophagy pathway in plants. In mammalian cells, it has been shown that ATG5 or ATG7 deficiency fails to block the formation of autophagosomes/autolysosomes for autophagy-mediated protein degradation upon induction by certain stressors, and thus macroautophagy can take place via at least two pathways: an ATG5/ATG7-dependent conventional pathway and an ATG5/ATG7-independent non-canonical pathway55. Our data raise the possibility that this might also hold true for plants and further study is needed to clarify this non-canonical pathway in plants.
Supporting the concept of the antiviral role of autophagy in potyviral infection, Nakahara and colleagues showed that the calmodulin-like protein in N. tabacum, rgs-CaM, counterattacks TEV HC-Pro and other VSRs by binding to the dsRNA-binding domain to inhibit its RNAi capacity and the resulting interaction complex is degraded by the autophagy pathway to enhance host antiviral defense56. Conversely, rgs-CaM in N. benthamiana suppresses RNA silencing and promotes geminivirus infection by autophagy-dependent degradation of suppressor of gene silencing 3 (SGS3), a key component of the RNA silencing pathway57. Recently, we also found that VPg, the second potyviral VSR, interacts with SGS3 and mediates the degradation of SGS3 and its intimate partner RNA-dependent RNA polymerase 6 (RDR6) via the ubiquitin–proteasome and autophagy pathways, suggesting a possible mechanism by which VPg sabotages host antiviral RNA silencing to promote viral infection58. The proviral role of autophagy has also been demonstrated during viral infection in animals15. For example, knockdown of ATG7 or Beclin1 inhibits the replication of Hepatitis C virus (HCV), demonstrating that they are proviral factors in HCV infection59,60. These data suggest that, during the co-evolutionary arms race, viruses have also developed strategies to subvert autophagy for their own benefit. Obviously, this increases the complexity of outcomes of induced autophagy in response to viral infection.
Based on the discussion above, we propose a model summarizing the possible role of autophagy in potyviral infection (Fig. 10). Viral infection activates autophagy, leading to the accumulation of high levels of ATGs. Beclin1 interacts with NIb at the GDD motif and directly suppresses its RdRp activity to restrict viral infection. The Beclin1–NIb complex is targeted for autophagic degradation possibly through the interaction of Beclin1 and ATG8a via AIM. In addition, the activated autophagy pathway also targets two VSRs, e.g., HC-Pro and VPg56,57. As an antiviral mechanism, the former is recognized by rgs-CaM to induce autophagy-dependent degradation56. The latter binds to SGS3 to mediate the degradation of SGS3 and RDR6 via autophagy, which promotes viral infection57. The mechanism(s) by which autophagy is recruited is not clear. Based on this study, targeting viral RdRp by Beclin1 is likely a general antiviral mechanism in plants. This opens up a novel exciting avenue for the control of plant RNA viruses through upregulation of Beclin1 and/or ATG8a or overexpression of the GDD-binding domain of Beclin1. It would be interesting to determine whether Beclin1 also interacts with RdRps of RNA viruses in mammalian cells and confers broad-spectrum resistance to viral infection therein.
Plant materials and growth conditions
N. benthamiana and Arabidopsis ecotype Col-0 seedlings were potted in soil and placed in an insect-free growth chamber under constant conditions of 60% relative humidity and a day/night regime of 16 h in the light at 22 °C followed by 8 h at 18 °C in the dark. The transgenic H2B-RFP line was a gift from Michael M. Goodin (University of Kentucky, USA). Arabidopsis mutants were obtained from Arabidopsis Stock Centre (ABRC at Ohio State University, Columbus, Ohio, USA): atg6 (SALK_109281), atg8a (SALK_045344c), vps15 (SALK_004719), pi3k (CS355912), atg2 (SALK_006994C), atg5 (CS39993), and atg7 (CS39995). AtATG6 overexpression (35S:Myc-AtATG6) and AtATG8a overexpression (35S:Myc-AtATG8a) transgene Arabidopsis seeds were obtained by using the flower-dip method61.
The recombinant viruses TuMV, TuMV-GFP, TuMV-ΔGDD, TuMV-CFP-NIb-6K2-mCherry, TuMV-6K2-mCherry, and PPV-GFP-mCherry were previously described35,36,62, and PePMV and CGMMV were isolated from tomato and cucumber in a greenhouse in Ontario, Canada.
GenBank accession numbers of genes and viral sequences analyzed in this study are as follows: NbBeclin1 (AY701316), NbPI3K (KX120977), NbVPS15 (KU561371), NbATG2 (KU561373), NbATG9 (KX369399), NbATG3 (KX369396), NbATG5 (KX369397), NbATG7 (KX369398), NbATG8a (KX120976), NbATG8f (KU561372), AtATG6 (NM_202746), TuMV (NC002509), TEV (NC001555), SMV (EU871724), PPV (KP998124), NbActin (AY179605), and AtActinII (AT3G18780). Gateway technology (Invitrogen, Burlington, Ontario, Canada) was used to generate all the plasmid constructs used in this study, unless otherwise stated. Gene sequences were amplified by PCR using Phusion® High-Fidelity DNA Polymerase (New England Biolabs, Pickering, ON, Canada) for cloning purposes. GoTaq® Flexi DNA Polymerase (Promega, Madison, WI, USA) was employed for regular PCR analysis. The full-length TuMV P1, HC-Pro, P3, P3N-PIPO, 6K1, CI, 6K2, VPg, NIa-Pro, NIb and CP, PPV NIb, SMV NIb, and TEV NIb were cloned previously30,32,33,62,63,64. NbBeclin1-, NbATG8a-, and NbATG8f-coding sequence were generated from cDNA derived from N. benthamiana leaves and AtATG6-coding sequence was generated from cDNA derived from Arabidopsis leaves (primers are listed in Supplementary Table 1). The GDD mutants of TuMV NIb and PPV NIb were cloned from the infectious clone TuMV-ΔGDD30 and PPV-ΔGDD36. Coding cDNA sequences of RdRp and its domains Met, Hel, and RdRp2 of CGMMV (GenBank accession: AB015146) and PepMV (KY031324) were cloned by RT-PCR. CGMMV RdRp2-ΔGDD, PepMV RdRp2-ΔGDD, TEV NIb-ΔGDD, and SMV NIb-ΔGDD were cloned by overlapping PCR using the specific primers (Supplementary Table 1). One AIM that matches the consensus amino acid sequence X-3X-2X-1-W/F/Y-X1X2-L/I/V was located in the N-terminal of NbBeclin1: EESFVVL. The mutant of AIM motif (NbBeclin1-NΔAIM) was produced by replacing EESFVVL with EESAVVA using overlapping PCR primers (Supplementary Table 1). The resulting DNA fragments were purified and transferred into the entry vector pDONR221 (Invitrogen) by recombination using BP Clonase® (Invitrogen) following the standard instruction by the supplier65. Insertions in the resulting pDONR clones were verified by DNA sequencing. For Y2H assays, inserts of the resulting intermediate pDONR221 clones were further transferred into modified Gateway-compatible vectors pGADT7-DEST (prey) or pGBKT7-DEST (bait)66 by recombination using LR Clonase® (Invitrogen) to yield pGAD-NbBeclin1, -AtATG6, -NbBeclin1-N, -NbBeclin1-C, NbBeclin1-NΔAIM, and pGBK-P1, -HC-Pro, -P3, -P3N-PIPO, -6K1, -CI, -6K2, -VPg, -NIa-Pro, -NIb, -NIb-N, -NIb-M, -NIb-C, NIb-ΔGDD, -CP, -PPV NIb, -PPV NIb-ΔGDD, -SMV NIb, SMV NIb-ΔGDD, -TEV NIb, -TEV NIb-ΔGDD, -NbATG8a, -NbATG8f, CGMMV RdRp, CGMMV Met, CGMMV Hel, CGMMV RdRp2, CGMMV RdRp2- ΔGDD, PepMV RdRp, PepMV Met, PepMV Hel, PepMV RdRp2, and PepMV RdRp2-ΔGDD, respectively. For the BiFC assay and transient expression analysis in plant cells, the full-length coding sequences of NbBeclin1, AtATG6, NbATG8a, NbATG8f, NIb, P3N-PIPO, CGMMV RdRp, CGMMV Met, CGMMV Hel, CGMMV RdRp2, PepMV RdRp, PepMV Met, PepMV Hel, and PepMV RdRp2, the partial coding sequences of NbBeclin1 (NbBeclin1-N and NbBeclin1-C) and NIb (NIb-N, NIb-M and NIb-C), and the mutants of NbBeclin1ΔAIM, NbBeclin1-NΔAIM, NIb-ΔGDD, CGMMV RdRp2-ΔGDD, and PepMV RdRp2-ΔGDD were introduced into the BiFC vectors pEarleygate201-YN or pEarleygate202-YC66 or binary destination vectors pEarleyGate101 (YFP in the C terminal), pEarleyGate104 (YFP in the N terminal), pEarleyGate102 (CFP in the C terminal)67, or pBA-Flag-Myc4 (Myc tag in the N terminal)68 to generate the corresponding plant expression vectors, respectively. To construct a TRV-based recombinant VIGS vector containing NbBeclin1, NbATG8a, NbATG8f, NbPI3K, NbVPS15, NbATG2, NbATG5, or NbATG7, a partial fragment of each gene was generated by PCR amplification with the respective primer pair (Supplementary Table 1) and then cloned into the pTRV2 vector using different restriction enzyme sites listed in Supplementary Table 1.
Agroinfiltration and viral inoculation
For transient expression analysis in N. benthamiana leaves, constructs were generated in Gateway-compatible binary vectors and transformed into A. tumefaciens strain GV3101 via electroporation. The 4–5 leaf stage wild-type N. benthamiana or H2B transgene N. benthamiana plants were used for Agrobacterium-mediated transient expression with minor modifications of our previous procedures62,63,64. For viral infection analysis, Agrobacterium cultures carrying different viral infectious virus clone(s) were infiltrated into N. benthamiana or Arabidopsis leaves and inoculated plants were photographed with a Canon 400D digital camera at different time periods. The negative control plants were agroinfiltrated with infiltration buffer as mock inoculations. The TuMV infection assay was repeated at least three times. For the TRV-VIGS assay, Agrobacterium cultures harboring pTRV1 and pTRV2-VIGS (TRV2-GUS, TRV2-NbBeclin1, TRV2-NbATG8a, TRV2-NbATG8f, TRV2-NbPI3K, TRV2-NbVPS15, TRV2-NbATG2, TRV2-NbATG5, or TRV2-NbATG7) were re-suspended in infiltration buffer (10 mM MgCl2 and 10 mM MES (pH5.6 and 100 μM acetosyringone) and mixed at a 1:1 ratio. After 3 h incubation at room temperature, the mixed Agrobacterium cultures were infiltrated into leaves of N. benthamiana plants at the 4–5 leaf stage. N. benthamiana plants infiltrated with TRV-based VIGS vectors targeting phytoene desaturase as an indicator, and silenced phenotype appeared in the upper leaves at 7 dpi. TRV-GUS- or TRV-VIGS-treated plants were agroinfiltrated with TuMV-GFP, CGMMV, or PepMV infectious clone at 7 dpi.
Y2H, BiFC, subcellular localization
Y2H was performed following the Clontech yeast protocol handbook. In brief, yeast cells (strain Y2H Gold, Clontech catalog number: 630498) carrying the co-transformed plasmids were plated onto a selective medium lacking tryptophan and leucine (SD/-Trp-Leu) to confirm the right transformation and a high-stringency selective medium lacking tryptophan, leucine, histidine, and adenine (SD/-Trp-Leu-His-Ade) to analyze the interaction. BiFC and subcellular localization experiments were performed as described32,35,62,63,64. At 36–72 h after infiltration, 1–2 cm2 leaf explants were excised for examining fluorescence in epidermal cells by confocal microscopy (Leica Microsystems, Wetzlar, Germany), equipped with a 63× water-corrected objective in multitrack mode. Light emitted at 680–720 nm was used to record chlorophyII autofluorescence. CFP was excited at 458 nm and captured at 470–500 nm, YFP was excited at 514 nm and captured at 565–585 nm, GFP was excited at 488 nm and captured at 510–550 nm, and mCherry or RFP was excited at 543 nm and captured at 590–630 nm. The sequential scanning mode was applied for co-imaging of different fluorescent proteins. Collected images were analyzed with the Leica Application Suite for Advanced Fluorescence (LAS AF, version 2.35) software (Leica Microsystem). Unless otherwise indicated, confocal images reported in the same figure panel were taken and processed with the same settings except otherwise indicated.
Protoplast isolation and TuMV replication assay
Mesophyll protoplasts were isolated and transfected essentially as described69. In brief, mesophyll protoplasts were prepared from 4-week-old wild type, atg6 (SALK_109281), 35S:Myc-AtATG6, atg8a (SALK_045344c), or 35S:Myc-AtATG8a Arabidopsis leaves or TRV-GUS-, TRV-NbBeclin1-, and TRV-NbATG8a-silenced newly emerged N. benthamiana leaves as described previously69. About 1×105 protoplasts were transfected with 20 µg pCambia2300-TuMV-GFP plasmid in 40% PEG 4000 in 0.8 M mannitol and 1 M CaCl2 at room temperature for 20 min. Transformed protoplasts were then washed and resuspended in W5 buffer (154 mM NaCl, 125 mM CaCl2, 5 mM KCl, and 2 mM MES, pH 5.7) and incubated for the virus replication. At 16, 24, and 48 h posttransfection, the protoplasts were harvested for the extraction of RNA. The protoplast transfection assay was repeated at least three times, and one representative result was used to plot a histogram.
RNA extraction and qRT-PCR analysis
Total RNA was extracted from mock (Agrobacterium-carrying empty vector)-, TuMV-ΔGDD-, or TuMV-infiltrated N. benthamiana leaves or Agrobacterium-carrying empty vector (Vec), NIb-YFP, Myc-NbBelin1 or Myc-NbBeclin1- and NIb-YFP-infiltrated N. benthamiana leaves or TRV-VIGS-treated N. benthamiana leaves or TuMV-GFP-, CGMMV-, or PepMV-infiltrated TRV-VIGS-treated N. benthamiana leaves or mock, TuMV-ΔGDD, TuMV, TuMV-GFP, CGMMV, or PepMV systemically infected Arabidopsis, N. benthamiana, or TRV-VIGS-treated N. benthamiana leaves at different time periods using the RNeasy Plant Mini Kit (Qiagen) and treated with DNase I following the manufacturer’s instructions. cDNA synthesized from reverse transcription of RNA samples was used to determine the mRNA expression levels of target genes as well as for quantifying TuMV accumulation levels at the date indicated. NbActin or AtActin II was used as an internal control for N. benthamiana and Arabidopsis, respectively. First-strand cDNA was synthesized from 1 µg total RNA by using Oligo(dT)12–18 primer or specific primers and SuperScript® III reverse transcriptase (Invitrogen) following the recommended protocol. A list of primers used in this study is provided (Supplementary Table 1). qRT-PCR was conducted and analyzed as described previously57,70.
Chemical treatments and TEM
Phosphate-buffered saline containing dimethyl dulfoxide (DMSO; control) or an equal volume of DMSO with 5 mM 3-MA, 100 μM E64d, or 1 μM Con A (Sigma) for inhibition of autophagy was infiltrated into leaves 8–12 h before samples were collected. For TEM observation, detailed information has been described previously57. Upper non-inoculated leaves of N. benthamiana plants infected with buffer (mock), TuMV, and TuMV-ΔGDD at 7 dpi or agro-infiltrated N. benthamiana leaves with mock, NbBeclin1, NbBeclin1ΔAIM, NbBeclin1-N, NbBeclin1-NΔAIM, or NbBeclin1-C at 60 hpi were pretreated with 10 mM 3-MA for 8 h and then were cut into small pieces (1 × 4 mm2). The treatments and the examination of the sampled tissues were performed as described57.
Immunoblotting and immunoprecipitation
Total protein was extracted from infiltrated leaf patches or mock-, TuMV-, and TuMV-ΔGDD-infiltrated or systemically infected N. benthamiana leaves as described previously32,33,57. Immunoblotting was performed with the primary rabbit polyclonal antibodies: GFP polyclonal antibody (1:5000; catalog number: ab6556), Myc polyclonal antibody (1:5000; catalog number: ab9106), and Beclin1 polyclonal antibody (1:1000; catalog number: ab62557) obtained from Abcam (Massachusetts, USA), ATG8 polyclonal antibody obtained from Millipore (1:2000; catalog number: ABC974, Millipore, Massachusetts, USA), and HA polyclonal antibody obtained from Sigma (1:10,000; catalog number: H6908, Sigma-Aldrich Canada Co., Oakville, ON, Canada), followed with goat anti-rabbit (1:10,000; catalog number: ab6721) secondary antibody conjugated to horseradish peroxidase (Abcam, Massachusetts, USA). Blotted membranes were washed thoroughly and visualized using chemiluminescence according to the manufacturer’s protocol (ECL; GE Healthcare). Immunoprecipitation was done on protein exacts from N. benthamiana leaves at 2 dpi by using GFP-Trap®_MA (Chromotek) according to the manufacturer’s instructions with some modifications. Briefly, about 1 g of leaf tissues were ground in liquid nitrogen and extracted with 2 ml lysis buffer [10% glycerol, 25 mM Tris HCl (pH 7.5), 1 mM EDTA, 150 mM NaCl, 2% w/v PVPP, 10 mM DTT, 1× EDTA-free protease inhibitor cocktail (Roche), 0.1% IGEPAL CA-630 (Sigma)]. After incubation on ice for 30 min with gentle shaking, the mixtures were centrifuged at 3000 × g at 4 °C for 10 min. The supernatants were then centrifuged with full speed at 4 °C for 10 min. Extracts were passed through a 0.45 µm filter and then incubated with GFP-Trap®_MA beads (ChromoTek) for 3 h at 4 °C with gentle shaking. The precipitations were washed four times with ice-cold immunoprecipitation buffer (10% glycerol, 25 mM Tris HCl (pH 7.5), 1 mM EDTA, 150 mM NaCl, 1× EDTA-free protease inhibitor cocktail (Roche), 0.1% IGEPAL CA-630) at 4 °C and analyzed by immunoblotting with Myc, HA, or GFP antibodies. Uncropped images of immunoblots are shown in Supplementary Figs. 19 through 26.
The authors declare that all data supporting the findings of this study are available in the manuscript and its Supplementary Information files or are available from the corresponding author upon request.
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We thank Michael M. Goodin (University of Kentucky) for the transgenic H2B-RFP line; Yule Liu (Tsinghua University) for the TRV VIGS vector; Yuhai Cui [Agricultural and Agri-Food Canada (AAFC)] for the modified gateway vectors of pGADT7, pGBKT7, p35S-YN, and p35SYC; Xiaoyun Wu (the Wang lab) for providing the PepMV infectious clone; Xiaofei Cheng and Hongguang Cui (AAFC) for helpful discussion and suggestions; Alex Molnar (AAFC) for photography and artwork; and Jamie McNeil (AAFC) for technical support. This work was supported in part by a GRDI grant and an A-base grant from AAFC and a discovery grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) to A.W.