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Three new phthalide derivatives from culture broth of Dentipellis fragilis and their cytotoxic activities

The Journal of Antibiotics volume 77, pages 338–344 (2024)Cite this article

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Abstract

Three new phthalide derivatives (1‒3) together with two known compounds, erinaceolactone B (4) and hericerin III (5), were isolated from the culture broth of Dentipellis fragilis. The chemical structures of 1‒5 were determined by analyses of their 1D-, 2D-NMR, and MS. The absolute configuration of 1 was determined by CD analysis. The isolated compounds were assessed for their cytotoxic activities against A549, DU145, HCT116, and HT1080 cancer cell lines. Compounds 1‒5 showed strong cytotoxic activities against DU145, with IC50 values ranging from 14.3 to 16.1 µM. Additionally, all compounds showed moderate or weak cytotoxic activities against all cell lines except for compounds 4 and 1 which showed no cytotoxic activities against A549 and HCT116 cancer cell lines, respectively. Against HT1080 cancer cell line, only compound 2 displayed moderate cytotoxic activity.

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Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2021R1I1A1A01055657). This paper was supported by research funds of Jeonbuk National University in 2023 and authors thank Ms. Ji-Young Oh, Center for University-wide Research Facilities (CURF) at Jeonbuk National University, for performing NMR measurements.

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Authors and Affiliations

  1. Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Jeonbuk National University, Iksan-si, 54596, Korea

    Dae-Won Ki, Dae-Cheol Choi, Young-Hee Kim, In-Kyoung Lee & Bong-Sik Yun

  2. Functional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, 56212, Korea

    Yeong-Seon Won & Seung-Jae Lee

  3. Division of Research Management, Department of Bioresource Industrialization, Honam National Institute of Biological Resource, Mokpo, 58762, Republic of Korea

    Yeong-Seon Won

  4. Department of Applied Biotechnology, University of Science and Technology (UST), Daejeon, 34113, Korea

    Seung-Jae Lee

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  1. Dae-Won Ki
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Correspondence to Dae-Won Ki or Bong-Sik Yun.

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Ki, DW., Choi, DC., Won, YS. et al. Three new phthalide derivatives from culture broth of Dentipellis fragilis and their cytotoxic activities. J Antibiot 77, 338–344 (2024). https://doi.org/10.1038/s41429-024-00720-8

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