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Actinopolymorphols E and F, pyrazine alkaloids from a marine sediment-derived bacterium Streptomyces sp

The Journal of Antibiotics volume 75, pages 619–625 (2022)Cite this article

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Abstract

HPLC-UV-guided fractionation of crude extract from the marine sediment-derived bacterium Streptomyces sp. CNP-944 has yielded two new pyrazine alkaloids, actinopolymorphols E and F (1 and 2), in addition to the previously reported biosynthetic product, actinopolymorphol G (3), and the known actinopolymorphol D (4). The chemical structures of 1−3 were determined based on the interpretation of the 1D, 2D NMR, and MS spectroscopic data. Compound 2 displayed weak antibacterial activities against Kocuria rhizophila, Bacillus subtilis, and Staphylococcus aureus with minimum inhibitory concentration (MIC) values of 16, 64, and 64 μg ml−1, respectively.

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Acknowledgements

This research was supported by the National Research Foundation of Korea grant funded by the Korean Government (Ministry of Science and ICT; no. 2021R1A4A2001251 to S-JN) and in part by the project titled “Development of Potential Antibiotic Compounds Using Polar Organism Resources” (15250103, KOPRI grant PM21030 to S-SC) funded by the Ministry of Oceans and Fisheries, Korea. Isolation of the bacterium strain Streptomyces sp. CNP-944 was a result of financial support from the US National Cancer Institute (grant CA R37044848 to WF).

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Author notes

  1. These authors contributed equally: Sohee Kim, Prima F. Hillman, Ji Young Lee.

Authors and Affiliations

  1. The Graduate School of Industrial Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul, 03760, Republic of Korea

    Sohee Kim

  2. Laboratories of Marine New Drugs, REDONE SEOUL, Seoul, 08594, Republic of Korea

    Sohee Kim & Jihye Lee

  3. Department of Chemistry and Nanoscience, Ewha Womans University, Seoul, 03760, Republic of Korea

    Prima F. Hillman, Ji Young Lee, Juri Lee, Jihye Lee, Sun-Shin Cha & Sang-Jip Nam

  4. Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul, 08826, Republic of Korea

    Dong-Chan Oh

  5. Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA, 92093-0204, USA

    William Fenical

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  1. Sohee Kim
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Kim, S., Hillman, P.F., Lee, J.Y. et al. Actinopolymorphols E and F, pyrazine alkaloids from a marine sediment-derived bacterium Streptomyces sp. J Antibiot 75, 619–625 (2022). https://doi.org/10.1038/s41429-022-00562-2

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