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Reply to ‘Comment to: Single-cell profiling reveals the trajectories of natural killer cell differentiation in bone marrow and a stress signature induced by acute myeloid leukemia’

Cellular & Molecular Immunology volume 18, pages 1350–1352 (2021)Cite this article

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We thank Melsen et al. for their interest in our study. Their remarks allow us to clarify key aspects of our work on human bone marrow NK cell biology.1

Our study reports the unsupervised single-cell analysis of NK cells from eight healthy donors and eight acute myeloid leukemia (AML) patients.1 First, we detected an adaptive NK cell subset that correlated with the cytomegalovirus (CMV) positivity status of the donors. In agreement with previous studies, not all CMV-seropositive donors possessed this additional NK cell population, at least at the transcriptomic level.2 We further showed that healthy human bone marrow NK cells encompassed three transcriptionally distinct populations in all donors, one CD56dim (hNK_Bm1) subset and two CD56bright (hNK_Bm2 and hNK_Bm3) subsets. Machine learning algorithms supported the robustness of our analysis and further validated this segregation.

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References

  1. Crinier A. et al. Single-cell profiling reveals the trajectories of natural killer cell differentiation in bone marrow and a stress signature induced by acute myeloid leukemia. Cell. Mol. Immunol. 18, 1290–1304 (2021).

  2. Schlums, H. et al. Cytomegalovirus infection drives adaptive epigenetic diversification of NK cells with altered signaling and effector function. Immunity 42, 443–456 (2015).

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Authors and Affiliations

  1. Aix Marseille University, CNRS, INSERM, Centre d’Immunologie de Marseille-Luminy, Marseille, France

    Adeline Crinier, Bertrand Escalière, Emilie Narni-Mancinelli & Eric Vivier

  2. APHM, Hôpital de la Timone, Marseille-Immunopôle, Marseille, France

    Eric Vivier

  3. Innate Pharma Research Laboratories, Innate Pharma, Marseille, France

    Eric Vivier

Authors
  1. Adeline Crinier
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  2. Bertrand Escalière
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  3. Emilie Narni-Mancinelli
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  4. Eric Vivier
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Corresponding authors

Correspondence to Emilie Narni-Mancinelli or Eric Vivier.

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Competing interests

E.V. is a cofounder and employee of Innate Pharma. The authors declare no competing interests.

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Crinier, A., Escalière, B., Narni-Mancinelli, E. et al. Reply to ‘Comment to: Single-cell profiling reveals the trajectories of natural killer cell differentiation in bone marrow and a stress signature induced by acute myeloid leukemia’. Cell Mol Immunol 18, 1350–1352 (2021). https://doi.org/10.1038/s41423-021-00678-9

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