Abstract
Pancreatic cancer is a malignant tumor of the digestive system. It is highly aggressive, easily metastasizes, and extremely difficult to treat. This study aimed to analyze the genes that might regulate pancreatic cancer migration to provide an essential basis for the prognostic assessment of pancreatic cancer and individualized treatment. A CRISPR knockout library directed against 915 murine genes was transfected into TB 32047 cell line to screen which gene loss promoted cell migration. Next-generation sequencing and PinAPL.py- analysis was performed to identify candidate genes. We then assessed the effect of serine/threonine kinase 11 (STK11) knockout on pancreatic cancer by wound-healing assay, chick agnosia (CAM) assay, and orthotopic mouse pancreatic cancer model. We performed RNA sequence and Western blotting for mechanistic studies to identify and verify the pathways. After accelerated Transwell migration screening, STK11 was identified as one of the top candidate genes. Further experiments showed that targeted knockout of STK11 promoted the cell migration and increased liver metastasis in mice. Mechanistic analyses revealed that STK11 knockout influences blood vessel morphogenesis and is closely associated with the enhanced expression of phosphodiesterases (PDEs), especially PDE4D, PDE4B, and PDE10A. PDE4 inhibitor Roflumilast inhibited STK11-KO cell migration and tumor size, further demonstrating that PDEs are essential for STK11-deficient cell migration. Our findings support the adoption of therapeutic strategies, including Roflumilast, for patients with STK11-mutated pancreatic cancer in order to improve treatment efficacy and ultimately prolong survival.
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Introduction
According to estimates from the Global Cancer Statistics [1], pancreatic malignancies claimed 466,003 lives in 2020, making it the sixth most common cause of cancer-related death among both sexes. The incidence of this disease has increased rapidly owing to its inconspicuous early symptoms, lack of diagnostic means, poor specificity of tumor markers, and frequent occurrence of early lymph node and distant metastases. Pancreatic cancer is highly metastatic even in the early stages, and local or metastatic recurrence can eventually lead to death. Indeed, approximately 50% of patients with pancreatic cancer already have distant metastases at the time of diagnosis, and the subsequent median survival is less than one year [2]. The most common types of pancreatic cancer therapy include surgery, chemotherapy, radiation therapy, targeted therapy, and supportive/palliative care. Surgery is still the only effective way to cure pancreatic cancer; however, the treatment eligibility rates are low.
Serine/threonine kinase 11 (STK11), also known as liver kinase B1 (LKB1), is associated with pancreatic cancer. STK11 was first shown to be mutated in Peutz–Jeghers syndrome and was later discovered to be an essential tumor suppressor [3]. An increasing amount of evidence suggests that inactivated STK11 somatic mutations contribute to the pathogenesis of numerous cancers, such as gastrointestinal cancer [4], non-small cell lung cancer (NSCLC) [5,6,7], pancreatic cancer [8], cervical cancer [9, 10], and melanoma [11], which are influenced by inactivated STK11 somatic mutations. To date, the mechanisms underlying STK11 loss-dependent migration are limited. However, some studies have highlighted the possible involvement of the STK11 pathway in metastasis as well as potential drugs and inhibitors. For example, STK11 increases the interaction of Snail with the E3 ligase FBXL14, thereby increasing ubiquitin-mediated Snail degradation in response to metformin treatment [8]. However, investigations of the mechanisms underlying STK11 loss-dependent migration are limited.
The primary treatment for metastatic pancreatic cancer is a combination of cytotoxic chemotherapies. The clinical significance of most genetic variants remains unclear, which limits the application of targeted therapy in the clinical diagnosis and treatment of pancreatic cancer. Targeted drugs commonly used for the treatment of pancreatic cancer include epidermal growth factor receptor (EGFR) inhibitors in patients with KRAS WT, poly (adenosine diphosphate-ribose) polymerase (PARP) inhibitors in patients with BRCA mutations and neurotrophic tyrosine receptor kinase (NTRK) inhibitors [12, 13]. The advent of targeted drugs has provided new treatment options for many patients with pancreatic cancer, significantly increasing their overall survival. STK11, a classic tumor suppressor, is mutated in pancreatic cancer and many other types of cancers. Somatic STK11 mutations have been observed in approximately 4% of sporadic PDAC [14,15,16,17]. However, there are still no effective targeted drugs for patients with STK11 mutations. Here, we identified PDE4, as potential drug targets in STK11-mutated pancreatic cancer.
PDE4, an enzyme that breaks down cyclic AMP in cells, influences various cellular processes such as inflammation, immune response, and cell proliferation [18]. These processes are critical in the development and progression of cancer, including pancreatic cancer. Roflumilast, as a PDE4 inhibitor, primarily known for its role in treating chronic obstructive pulmonary disease (COPD), could be a valuable therapeutic agent in cancer treatment, especially in specific types like B-cell malignancies and lung cancer [19, 20]. In summary, the application of Roflumilast on STK11-mutant pancreatic cancer cell lines, as explored in this study, lays a foundational platform for future clinical research. This could potentially offer substantial benefits to patients with such specific cancer profiles. The insights gained from these investigations are crucial for developing novel therapeutic strategies for treating STK11-mutated pancreatic cancers, where innovative and effective treatments are urgently needed.
Results
Functional selection of cell migration regulators in PDAC
To investigate cell migration regulators in PDAC, we prepared 450,000 library cells in serum-free medium, distributed over three Transwell FluoroBlok inserts. After 6 hours, we isolated a subset (1% of the total) from the lower compartments, which showed accelerated migration (Fig. 1a). This method allowed focused analysis of cells with enhanced migratory behavior in PDAC. In the supplemental table of full sgRNA screening results and Fig. 1b, protein kinase cAMP-dependent type I and type II regulatory subunit alpha (Prkar1a and Prkar2a) and Stk11 were identified as the top positive genes following Transwell selection (Avg logFC > 1, analyzed using PinAPL-). After screening, only 125 genes had one or more good sgRNAs (Fig. 1c). Three of the five Stk11-targeting sgRNAs were found to be highly enriched after screening (Fig. 1d). The basal expression of STK11 was detected using western blotting (Fig. 1f). STK11 was almost completely lost in TKCC-10 and Mayo4636 cell lines, whereas it was highly expressed in the PANC-1 cells.
STK11 knockout promotes cell migration in vitro
Cellular migration was significantly increased in association with low STK11 expression in TB 32047 and PANC-1 cells (Supplementary Fig. 1). To further determine the role of STK11 in PDAC migration, STK11 knockout (KO) was generated in five PDAC cell lines (Fig. 2a–d; Supplementary Fig. 5o, p, q). All knockouts were verified by Sanger sequence to determine the type of mutation (Supplementary Table 5). IncuCyte® scratch wound assay showed that STK11KO significantly promoted PDAC cell motility (Fig. 2e, f). For the TB 32047 cell line, TB KO1.5, and TB KO2.2 healed 73.39% and 98.84% of the wound, respectively, after 24 h, whereas WT and NC1.4 cells healed less than 35% of the wound area (Fig. 2g). In the PANC-1 cell line, PANC KO1.2 and PANC KO4.4 healed 72.81% and 85.76% of the wound, respectively, after 24 h, whereas control cells healed less than 60% of the wound area (Fig. 2h).
STK11-deleted clones show pronounced tumor aggressiveness in vivo
A chicken CAM xenograft assay was performed to confirm the more aggressive and invasive phenotype of TB 32047 cells (Fig. 3). Representative overview images of the different growth patterns of tumors are shown in Fig. 3a. Only TB KO1.5, showed a significantly larger tumor than the control group (Supplementary Fig. 2). However, tumors formed by TB KO1.5 and TB KO2.2 also showed aggressive growth, which could be observed by simple macroscopic and microscopic investigation of CAM microtumors (Fig. 3b–e). A transparent or intact CAM layer was observed in the control group when tumor sections stained with H&E and Pan-cytokeratin were evaluated. Compared to controls, TB KO1.5 and TB KO2.2 cells significantly damaged the CAM layer, with highly infiltrative growth at the invasive tumor front and considerable interaction/mixing with the CAM tissue throughout.
STK11 inhibited PDAC metastasis in vivo
An orthotopic pancreatic cancer model was used to investigate the effects of STK11 on the formation of pancreatic tumors and liver metastases in C57/BL6 mice [21]. Loss of STK11 significantly increased the tumor volume and weight (Fig. 4a–c). Metastases were macroscopically observed in 1 of 15 mice injected in the control group (TB 32047 WT and NC1.4) and in 9 of 11 mice injected with STK11KO cells (TB KO1.5 and TB KO2.2) (Fig. 4d, p = 0.0007). The presence of metastatic tumors was confirmed by H&E staining of sectioned liver tissues obtained from the mice (Fig. 4e).
Loss of STK11 preferentially targets blood vessel morphogenesis, while also altering the expression of PDEs in TB 32407 and PANC-1 cells
RNA sequencing was utilized to compare the STK11KO group, encompassing three distinct knockout variants, with the control group that comprises the wild type and two different negative control cells. Principal component analysis (PCA) based on the expression of the differentially expressed genes (DEGs) confirmed that most of the variance in the data of TB 32047 cells (37%, PC1) and PANC-1 cells (43%, PC1) was associated with changes in expression between STK11KO and control (WT and NC) samples (Supplementary Fig. 3a, b). All differentially expressed genes (adj p < 0.05) were visualized to assess sample consistency (Supplementary Fig. 3c–h). GO analysis revealed a significant enrichment of genes involved in blood vessel morphogenesis, angiogenesis, and inflammatory response in STK11KO TB 32047 cells (Fig. 5a), blood vessel morphogenesis, response to wounding, and blood vessel development in STK11KO PANC-1 cells (Fig. 5b).
The most upregulated genes in murine STK11KO cells were SRY box transcription factor 17 (Sox17), interleukin 33 (Il33), and decay-accelerating factor (Cd55) (Fig. 5c, f and Supplementary Fig. 4a–d). Eph receptor B3 (Ephb3), cellular communication network factor 2 (Ccn2), integrin alpha 7 (Itga7), and integrin subunit beta 3 (Itgb3) are also essential genes involved in blood vessel morphogenesis and angiogenesis signaling and play a critical role in the metastasis of PDAC. Ephb3, Itga7, and Itgb3 mRNA levels increased significantly in STK11KO TB 32047 cells (Supplementary Fig. 4e, f, h). STK11 expression was positively correlated with Ccn2 (Supplementary Fig. 4g). The most upregulated genes in human STK11KO cells were interleukin 11 (IL11), microtubule-associated protein 7 (MAP7), and CD55 (Fig. 5d and Supplementary Fig. 4k–n). Murine and human STK11KO cells shared a significant proportion of 220 transcripts, including 42 upregulated and 78 downregulated transcripts including CD55, PDE4D, and PDE10A (Fig. 5e). Gene ontology enrichment score analysis showed that the loss of STK11 promoted PDAC migration and metastasis through blood vessel morphogenesis and angiogenesis pathways.
Integrated analysis of the transcriptomes of TB 32047 and PANC-1 cell lines revealed STK11-driven regulation of PDEs. STK11 knockout was selectively effective in increasing the expression of PDE4D (Fig. 5f and Supplementary Fig. 4i, o). In STK11 knockout cells, the mRNA level of PDE4D changed more than four times, but the mRNA level of PDE10A changed more than a thousand times (Supplementary Fig. 4i, j). The basal expression of PDE10A in TB 32047 cells was below the detection limit and STK11 deletion may activate PDE10A transcription via an unknown mechanism. However, PDE4B expression was not detected in WT or STK11KO TB 32047 cells. In PANC-1 cells, the expression of PDE4B in STK11KO cells at the mRNA and protein levels increased significantly, whereas PDE10A expression did not change significantly (Supplementary Fig. 4p, q).
Next, the above-mentioned targets were validated in additional PDAC cell lines (Supplementary Fig. 5). The expression levels of Sox17, IL33, CD55, and PDE4D were also significantly elevated after STK11 knockout in KPC 792 cell line (Supplementary Fig. 5a–e, o). PDE4B, but not MAP7 or CD55, was highly expressed in STK11-deleted SUIT2 cells (Supplementary Fig. 5f–i, p). PDE4D, PDE4B, PDE10A, and CD55 were highly expressed in STK11-deleted SU86.86 cells (Supplementary Fig. 5j–n, q). These results indicate that PDEs are the most promising targets among several STK11 mutant PDAC cell lines.
STK11 KO cell migration regulation by PDE inhibitors in PDAC
The viability of WT and STK11KO cells was not affected by the PDE4 inhibitors (Fig. 6a, c and Supplementary Fig. 6c). However, cell migration decreased significantly in STK11KO cells but not in WT TB 32047 cells after treatment with Roflumilast (50 μM) (Fig. 6b, e). In PANC-1 cells, after treatment with Rolipram and Roflumilast, cell migration was significantly decreased in STK11KO cell lines, whereas not in PANC-1 WT cells (Fig. 6d, f and Supplementary Fig. 6d). Because of the high expression of PDE10A in STK11KO TB 32047 and SU86.86 cell lines, Mardepodect (a PDE10A inhibitor) was used (Supplementary Fig. 6a, b, i, j). In the TB 32047 and SU86.86 cell lines, Mardepodect not only reduced the migratory ability of STK11KO cells, and showed more toxic to STK11KO cells than WT and NC. The PDE4 inhibitor also exerted an inhibitory effect on the migration of STK11KO SUIT2 and SU86.86 cells (Supplementary Fig. 6e–h, j–l). This suggested that PDEs play an essential role in the migration of STK11KO PDAC cells.
Based on these observations, we examined the effects of Roflumilast in C57BL/6 mice. Relative pancreatic weight and representative images of H&E staining of pancreatic tissues showed that Roflumilast hampered PDAC progression in the STK11KO group (Fig. 6g, h and Supplementary Fig. 7a, c), although liver metastasis was not significantly reduced (Fig. 6i and Supplementary Fig. 7b).
Discussion
Large-scale CRISPR/Cas9 screening efforts to inhibit gene expression have revolutionized genetic screening, enabling previously impossible discoveries [22]. Herein, we present a protein-kinase-wide screening method that reveals known and novel mechanisms of metastasis in PDAC. A lentivirus-based sgRNA library targeting mouse protein kinase was used to analyze protein-kinase-wide loss-of-function by stable gene knockout. Although screening for cell migration regulators by Transwell compartments has previously been reported [23], in this study, we used CRISPR/Cas9 technology instead of RNAi-based functional selection. Several cell migration-regulating genes were identified in the present study have been reported previously, thus validating the selection approach. For example, Prkar1a knockout in the pancreas leads to neuroendocrine tumorigenesis. Δ-Prkar1a mice showed local invasion, neoplastic emboli in the lymphatic vessels, and occasional metastases to the locoregional lymph nodes in histological analyses [24]. Several studies have highlighted a possible involvement of the STK11 pathway in metastasis. In lung cancer, STK11 knockdown has been reported to increase cell motility and invasiveness and influence many epithelial–mesenchymal transition (EMT) pathway marker proteins, such as ZEB1 and E-cadherin [25]. Vimentin, a marker of the EMT pathway, was analyzed in STK11KO cells (Supplementary Fig. 8) and its expression was found to be significantly higher in STK11-deficient PANC-1 and SUIT2 cells than in the WT and NC groups. Moreover, STK11 deficient cells have been reported to exhibit impaired DNA damage [26]. Consistent with this information, the absence of STK11 enhanced cell sensitivity to IR and decreased colony formation and cell survival compared with wild-type STK11 cells (Supplementary Fig. 9). Therefore, STK11 mutation might be a potential biomarker for the selection of patients for radiation therapy.
In lung cancer, STK11 loss causes a metastasis-like subpopulation of cancer cells in primary tumors and metastases to activate the early endodermal transcription factor, Sox17. In STK11-deficient cells, Sox17 expression is essential and sufficient to trigger a second wave of epigenetic alterations that improve metastatic potential [27], which is consistent with the elevation of Sox17 expression in STK11KO PDAC mouse cell lines (Supplementary Figs. 4b and 5b, o). However, this correlation has only been observed in mouse PDAC cell lines, with inconsistent results in human PDAC cell lines. The same applies to the effect of STK11 deletion on IL33 (Supplementary Figs. 4c and 5c, o). The deletion of STK11 significantly increased IL33 mRNA and protein levels in mouse PDAC cell lines. However, in the PANC-1 cell line, the mRNA level of IL11 not IL33 increased in STK11KO (Supplementary Fig. 4n). The difficulty in targeted STK11 therapy is well illustrated by the specificity of the downstream targets of STK11 in different cell lines. The different genetic backgrounds, gene expression profiles, and mutational profiles of different cell lines add to the complexity of this study.
PDE4D, PDE4B, and PDE10A are the PDEs. PDE enzymes with 11 isoforms catalyze the hydrolysis of cyclic nucleotides cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP). PDE4 was the largest and one of the first discovered PDE families. It contains four genes (PDE4A, PDE4B, PDE4C, and PDE4D) and is a cAMP-specific phosphodiesterase [28]. Among several STK11-specific signatures, PDE4B, PDE4D, and PDE10A were upregulated, depending on whether the STK11 gene was a mutant. A significant increase in PDE10A caused by STK11 knockout was observed in TB 32047 and SU86.86 cell lines, a significant increase in PDE4B caused by STK11 knockout was observed in three human PDAC cell lines, and a significant increase in PDE4D caused by STK11 knockout was observed in two mouse and two human PDAC cell lines. Owing to the variability in different cell lines, STK11 knockout regulates different PDE isoforms in different PDAC cell lines, which may be related to the basal expression of PDEs in different cell lines.
Understanding the complicated mechanisms that drive the spread of cancer will provide crucial information for therapeutic interventions. Recently, an association between PDE4 inhibitors and cancer therapy was reported. PDE4 inhibitors can overcome tumor resistance and decrease human glioblastoma cell survival [29] and lung cancer. PDE4-specific inhibitors inhibit proliferation and growth of STK11-mutated cells [30]. Roflumilast was the first PDE4 inhibitor approved by the FDA for the prevention of chronic obstructive pulmonary disease (COPD) exacerbations [31] and effectively inhibits tumor proliferation and growth in ovarian cancer [32]. Roflumilast may be related to the prevention of enhanced cAMP-mediated inflammatory processes and angiogenesis indicators in tumor tissues [19]. In this study, because of the high expression of PDE4B and PDE4D in STK11-mutated PDAC cells, two different PDE4 inhibitors, Rolipram and Roflumilast, were used to treat the cells. After treatment, cell migration decreased significantly in the three STK11 mutant human PDAC cell lines, and no significant decrease in cell migration was observed compared with most vehicle controls. Mardepodect, a specific PDE10A inhibitor, significantly decreased cell migration in STK11 mutant TB 32047 and SU86.86 cell lines. In mice with STK11 mutant tumors, Roflumilast treatment reduced the number of liver metastases; however, a significant reduction was not achieved compared to that in the non-treated group. However, there was a significant reduction in the tumor weight in the Roflumilast-treated group (Fig. 6g, h). Considering the better metastasis suppression effect of Roflumilast on STK11-mutated human-derived PDAC cell lines than on mouse-derived PDAC cell lines in vitro (Fig. 6b, d and Supplementary Fig. 6g, j), maybe better results can obtain in NSG mice (NOD scid gamma mouse). Recent research has discovered that targeting PDE4D can inhibit the development of pancreatic cancer tumors in NOD mouse models, further confirming our hypothesis [33].
Although more mechanistic studies are required to understand the relationship between STK11 and PDEs in the progression of PDAC, the results reported herein also indicate a potential path for therapeutic intervention with Roflumilast for STK11 mutant pancreatic cancer patients. The pharmacological profile of Roflumilast makes it a potential targeted drug with promising clinical applications.
Materials and methods
Cell culture
PANC-1 (CRL-1469TM, RRID: CVCL_0480) cell line was purchased from ATCC® (American Type Culture Collection) and grown in Roswell Park Memorial Institute Medium 1640 supplemented with 10% (v/v) fetal bovine serum (FBS). SUIT2 cell line was purchased from the Japanese Collection of Research Bioresource Cell Bank (JCRB1094, RRID: CVCL_3172) and grown in a minimum essential medium supplemented with 10% (v/v) FBS. SU86.86 (CRL-1837TM, RRID: CVCL_3881), Miapaca2 (RM-CRL-1420TM, RRID: CVCL_0428), Aspc1 (CRL-1682TM, RRID: CVCL_0152), and Panc 05.04 (CRL-2557TM, RRID: CVCL_1637) cell lines were obtained from ATCC®. The TKCC-10 [34] and Mayo4636 [35] cell lines were gifts from David Chang and Debabrata Mukhopadhyay, respectively. TB 32047 [36], obtained courtesy of Prof. David Tuveson, Cold Spring Harbor Laboratory, was grown in Dulbecco’s modified Eagle’s medium with 10% (v/v) FBS. KPC 792 [37], was obtained from Marc P. Stemmler. HEK293TN cells (CRL-3216TM, RRID: CVCL_UL49), also obtained from ATCC®, were grown in DMEM supplemented with 10% of inactivated FBS. Cells were maintained at 37 °C in a 5% CO2. Additional information regarding the cell culture media is provided in Supplementary Table 1. All the media were free of penicillin/streptomycin. DNA fingerprinting using highly polymorphic short tandem repeat (STR) analysis was used to authenticate the cells, and mycoplasma testing was performed regularly to ensure that the cells were free of mycoplasma contamination.
Generation of the CRISPR/Cas9 lentiviral library and screening
The mouse ACF CRISPR knockout library included a pooled mouse Brie kinome library, which was a gift from John Doench and David Root (Addgene plasmid #75316), and additional sgRNAs designed for genes involved in pancreatic ductal adenocarcinoma (PDAC). The library contains 3466 unique sgRNAs targeting 915 genes. ACF CRISPR knockout library virus particles were generated in T75 flasks. HEK293TN cells were co-transfected with the 4.3 µg sgRNA library plasmid, 2.8 µg pMDLg/pRRE (Addgene plasmid #12251), 1.4 µg pRSV-REV (Addgene plasmid #12253), and 1.4 µg pMD2.G (Addgene plasmid #12259) using Lipofectamine 3000 transfection reagent, and viral supernatants were collected after 24 h. TB 32047 Cas9 cells were transduced with the virus at an MOI of 0.3. After a few days of steady growth, 450,000 library cells resuspended in serum-free medium were divided and placed on three Transwell FluoroBlok culture inserts (Corning, #351152, 8.0 μm), and the lower Transwell chambers were filled with medium containing 10% FBS. After 6 h of incubation, cells that migrated to the lower faces of the inserts were collected by trypsinization. To identify integrated sgRNAs in the migrated cells, 10 million cells were collected for genomic DNA isolation.
RNA sequencing and Gene Ontology enrichment analysis
Total RNA was extracted from the TB 32047 and PANC-1 cells. RNA quality check and then libraries were pooled and sequenced on an Illumina Hiseq 2500 platform. The reads were aligned to the GRCm38.6 mouse reference genome or the GRCh38.6 human reference genome using STAR aligner (RRID: SCR_004463, version 2.7.8. a). Unique mappings were counted using featureCounts (version 2.0.1) when overlapping exons were from the Ensembl gene model (Mus musculus, version 102). Counting of reads per gene also uses strand information about the library molecule alignments to separately count overlapping genes with different strands. Normalized read counts were used for downstream analysis. Statistical analysis to identify differentially expressed genes between the compared groups was performed using the DESeq2 package (RRID: SCR_000154, version 1.30) in the R statistical environment (version 4.0.3) using default parameters. Heat maps were used to visualize the results. Gene ontology (GO) describes gene products with three independent categories: biological processes, cellular components, and molecular functions. GO was determined using R package clusterProfiler (RRID: SCR_016884, version 3.16.1). Differentially expressed protein-coding genes (|log2(Fold Change)| >1) were included, and p < 0.05 was considered significant for the enrichment of biological processes.
CAM assay
Fertilized and pathogen-free eggs (VALO BioMedia) were used in the CAM assay. The eggs were opened on the flattened pole on day 8 of embryonic development and the eggshell membrane was removed. On day 9, the TB 32047 cells were resuspended in a Matrigel–medium (1:1) mixture. Pipetted drops of the Matrigel/cell solution were placed in sterile culture dishes. One Matrigel/cell pellet was carefully placed in the CAM of each egg. The eggs were then incubated for another five days. The samples were further incubated at RT in 4% formalin solution more than 24 h and processed to create formalin-fixed, paraffin-embedded (FFPE) blocks. FFPE sections were stained with hematoxylin and eosin (H&E) and pan-cytokeratin. Five randomly selected high-power fields of each sample were used to analyze the characteristics of the morphological cells. The sample volume was calculated using the following equation: Volume = length×width×height×0.52 [38].
Genomic DNA isolation and PCR amplification
Genomic DNA was extracted using NucleoSpin® Blood XL (Machery Nagel, #740950.50), according to the manufacturer’s protocol. Next, 10 μg of genomic DNA isolated from the migrated and control cells (mouse ACF CRISPR knockout library cells) was subjected to PCR. sgRNA segments were amplified as described previously [39]. The PCR reactions (100 μL) included 10 μg of DNA, 50 μL of Q5® Hot Start High-Fidelity 2X Master Mix (NEB, #M0494L), 3 μL of a 10 μM solution of P5 (forward primer: ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNNTCTTGTGGAAAGGACGAAACACCG), and 3 μL of a 10 μM solution of P7 (reverse primer: GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTTCTACTATTCTTTCCCCTGCACTGT). The diluted PCR product was prepared on an Illumina Hiseq 2500 platform at the Technische Universität Dresden (TU Dresden) deep sequencing facility. Raw FASTQ files were analyzed using PinAPL-Py software [40].
Construction of stable knockout cell lines by CRISPR/Cas9
STK11 knockout cell lines were generated using the SpCas9 (BB)-2A-Puro vector (Addgene plasmid #48139), which includes the Cas9 protein. First, two good sgRNAs targeting STK11 were selected from screening data for synthetic oligos. sgRNA oligos in SpCas9 (BB)-2A-Puro were used to generate the pSpCas9(BB)-2A-Puro-STK11. pSpCas9 (BB)-2A-Puro-STK11-specific plasmids were transfected into TB 32047, KPC 792, PANC-1, SUIT2, or SU86.86 cells using Lipofectamine 3,000 reagent according to the manufacturer’s protocol. After 24 h of transfection, cells were selected using 10 µg/ml puromycin for 3 days. Oligonucleotides targeting STK11 are listed in Supplementary Table 2. The number of transfected cells was counted and 80 cells were diluted in 12 ml of medium in each 96-well plate. The correct single-clone cells were selected under a microscope after 5–10 day. The selected clones were verified using RT-qPCR, western blotting, and Sanger sequencing.
Wound-healing assay
An appropriate volume of working cell solution was prepared at a density of 3–5 × 105 cells/ml and seeded at a volume of 100 μl/well in 96-well plates, 500 μl/well in 24-well plates, and 1 ml/well in 12-well plates. When the cells reached 98% confluence, the medium was exchanged with free serum medium (TB 32047 or SU86.86 cell line), 1–2% serum medium (PANC-1, KPC 792, or SUIT2 cell line), or PDE inhibitors for 6–36 h for starvation or inhibition, as described previously [41]. A wound was then created using a vacuum pump, wound-maker, or pipette tip. Any remaining medium was removed from the edges of the wells and the cells were washed with PBS. PBS was then replaced with free FBS medium (TB 32047 or SU86.86 cell line), 1–2% serum medium (PANC-1, KPC 792, or SUIT2 cell line), or medium with inhibitor for 24—36 h. Using EVOS microscopy, images of each complete wound were captured centered in the field of view.
RNA extraction and RT-qPCR analysis
Total RNA was extracted from the cell lines using the NucleoSpin® RNA Plus kit (MACHEREY-NAGEL, #740984.250). cDNA was synthesized using a high-capacity cDNA reverse transcription kit (Applied BiosystemsTM, #4368814). GAPDH or β-actin was used as an internal control to quantify the mRNA levels of other genes. The sequences of gene-specific primers are listed in Supplementary Table 3. Relative mRNA levels were determined by RT-qPCR on a Light Cycler 480 II using the SYBR Green method (Applied BiosystemsTM, #4367659). Three biological replicates were used for each experiment. The relative mRNA expression levels were calculated using the 2 − ∆∆Ct method.
Establishment of the PDAC 3D organoid model
A total of 5000 cells were resuspended in 50 μl Matrigel, seeded in 6-well plates as a dome, and solidified at 37 °C for 30 min. A maximum of six domes was plated in each well. A feeding medium was added, and the cells were incubated at 37 °C for 7–10 days. After sphere formation, the supernatant was discarded. The domes were collected using a cell recovery medium (Corning, # 342053) and placed on ice for 30 min. Enzyme digestion was then performed with a mixture of organoid digestion medium (Supplementary Table 1; 1 ml per six domes). The domes were then incubated for 5 min at 37 °C and centrifuged at 250 ×g at 4 °C. Next, 2 ml of washing medium was rinsed over the cell pellets to digest as many spheroids as possible. The cells were counted using an automated cell counter and centrifuged at 250 ×g for 5 min at 4 °C. The supernatant was removed and 40,000 cells were resuspended in 20 μl phenol-red-free Matrigel (Corning, #356231).
Sanger sequencing
Sanger sequencing can be used to detect the targeted genomic modifications. Genomic DNA was extracted using NucleoSpin® tissue (Machery Nagel, #740952.50) according to the manufacturer’s protocol for cultured cells. Two oligos were designed to target the modified regions using Primer Premier 5. The primers designed for the knockout check are listed in Supplementary Table 4. Amplicons were subcloned into a plasmid using the NEB® PCR Cloning Kit (NEB, #E1203S) for transformation and several individual colonies were selected and sequenced to determine the clonal genotype (Supplementary Table 5).
X-irradiation
cells were divided into 100, 200, 300, 800, 1500, 2500, and 5000 per well. After 24 h, cells were separately exposed to 0, 1, 2, 4, 6, 8, or 10 Gy X-rays using a Siemens Primus Accelerator machine (6 Mv; Siemens AG, Munich, Germany) and cultured for 7–12 days. The colonies were fixed with methanol and stained with 0.5% crystal violet. More than 50 cells were counted as a colony. The efficiency of clone formation was calculated and a survival curve was generated by comparison with the untreated group.
Immunofluorescence imaging
Cells were grown in 4-well chamber slides, fixed with 4% paraformaldehyde, and incubated overnight with primary antibodies or Isotype Normal Rabbit IgG (R&D System, AB-105-C). Proteins were visualized by incubation with Alexa FlourTM 488 goat anti-rabbit IgG (Invitrogen, #A11034; Invitrogen). Nuclei were visualized by incubation with DAPI (1:5000). The coverslips or slides were covered with a mounting medium (Dako, #S302380). Fluorescence was monitored with a Leica TCS SP8 confocal laser microscope (Manheim, Germany) and processed using Leica Application Suite X v2.0.1.14392. The antibodies used are listed in Supplementary Table 6.
Western blot
Cells were lysed using radioimmunoprecipitation assay buffer (RIPA) and phosphatase inhibitor cocktail (1:100). Protein concentration was determined photometrically using a Pierce® BCA Protein Assay Kit (Thermo Fisher, #23225). Gel electrophoresis was performed using BoltTM 4–12% Bis-Tris Plus gels (Thermo Fisher, #NW04122BOX), and the proteins were transferred to a nitrocellulose membrane. The membrane was incubated in a blocking solution for 1 h at room temperature. The primary antibody was diluted with 5% milk or bovine serum albumin (BSA), and the membrane was incubated in the primary antibody solution overnight at 4 °C with gentle rocking. The membrane was further incubated with 5% milk-diluted secondary antibodies for 1 h at room temperature, with gentle rocking (Supplementary Table 6). Signals were detected using an AmershamTM Imager 600 with Signal FireTM (Elite) ECL reagent (Cell Signaling Technology, #6883 or #12757). All western blotting assays were performed at least thrice to validate the reliability of the results.
Establishment of an orthotopic murine pancreatic cancer model and Roflumilast treatment
All animal studies met the relevant ethical considerations and were monitored according to the German legislation on animal protection and the Guide for the Care and Use of Laboratory Animals [42]. C57BL/6JRj (Black 6) mice, without gender selection and aged 7 weeks, were acclimated to the animal facility for one week. Animals were randomly divided into groups (n = 5 per group). The pancreas was extracted and held in the tail to extend it, and then 4×104 spheres resuspended in 20 μl Matrigel were injected directly into the body of the pancreas [43], superior to the visible large blood vessels. After three weeks, the pancreas and livers were excised for further pathological examination.
In all, 4 × 104 spheres mixed with 20 μl Matrigel were injected directly into the body of the pancreas for treatment with Roflumilast (Hycultec, #HY-15455). After one week, mice were treated intraperitoneally with Roflumilast (5 mg/kg/day) or vehicle as indicated. After 16 days of treatment, the mice were killed and the pancreas and livers were collected for further evaluation. Tumor volume was measured with calipers and calculated using the following formula: volume = length × width × height × 0.52 [38].
Statistical analysis
Statistical tests were performed using GraphPad software (RRID:SCR_002798, v.7) to compare groups under different conditions with replicates. In all tests, statistical significance was established as *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. T-test and one-way and two-way ANOVAs were used for cell culture experiments. Fisher’s exact test was used for mouse experiments.
Data availability
Raw sequencing files were deposited at the SRA under accession number PRJNA960707. All other data are available in the article and Supplementary Information. Source data were provided in this study.
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Acknowledgements
The present work was performed in fulfillment of the requirements for obtaining a degree from Dr. rer. biol. hum. of Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU) by Shuman Zhang. This research was funded by the EU (MSCA-ITN 1705 861196, PRECODE; CP) and the Chinese Scholarship Council (CSC No. 201806880012, SZ; CSC No. 202108080198, YZ). PC and PNG are supported by Halls Møller grant from Novo Nordisk and by the Interdisziplinäre Zentrum für Klinische Forschung, Erlangen. CP thanks Ronald Belford Scott for his support.
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CP supervised the study, conceived the project, and designed the experiments; SZ performed the majority of experiments, acquired the data, and conducted data analyses and interpretation; DY contributed to bioinformatic analysis; GD, ABE, and CB contributed to RNA sequencing analysis; ME, ID, YZ, YL, HH, JZ, and EL assisted in the mouse experiments and pathological analysis; HY contributed to the deep sequence analysis; HY, CH, and RSS contributed to the CAM assay; BF contributed to the radiation assay; PC and PNG contributed to the IncuCyte® migration assay; ID, SP, SZ, BL, and NBL contributed technical assistance; MPS, AD, DM, DC, AB gifted the cells; SZ wrote the draft; CP revised the manuscript; supervision by RG and CP. All authors discussed and commented on the manuscript. All authors have read and approved the final manuscript.
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Zhang, S., Yun, D., Yang, H. et al. Roflumilast inhibits tumor growth and migration in STK11/LKB1 deficient pancreatic cancer. Cell Death Discov. 10, 124 (2024). https://doi.org/10.1038/s41420-024-01890-y
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DOI: https://doi.org/10.1038/s41420-024-01890-y