Abstract
Long non-coding RNAs (LncRNAs) have played very important roles in the malignancy behaviors of hepatocellular carcinoma (HCC). LncRNA LOC554202 (LOC554202) was a newly identified tumor-related lncRNA. However, its expression and function in HCC remained unknown. In this study, we firstly reported that LOC554202 expression was distinctly upregulated in HCC specimens and cell lines. Clinical assays indicated that increased LOC554202 expression had a diagnostic value for HCC patients and was positively associated with advanced stages and poor clinical prognosis. Additionally, forkhead box O3(FOXO3) could bind directly to the LOC554202 promoter region and activate its transcription. Functionally, we observed that knockdown of LOC554202 suppressed the proliferation, migration, invasion, and epithelial–mesenchymal transition (EMT) progress of HCC cells, and promoted apoptosis. Mechanistically, LOC554202 competitively bound to miR-485-5p and prevented the suppressive effects of miR-485-5p on its target gene basigin (BSG), which finally led to HCC metastasis, EMT, and docetaxel chemoresistance. Our data demonstrated that FOXO3-induced LOC554202 contributed to HCC progression by upregulating BSG via competitively binding to miR-485-5p, which suggested that the regulation of the FOXO3/LOC554202/miR-485-5p/BSG axis may have beneficial effects in the treatment of HCC.
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Data availability
The data used to support the findings of this study are available from the corresponding author upon request
Change history
21 April 2022
A Correction to this paper has been published: https://doi.org/10.1038/s41417-022-00468-z
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Acknowledgements
This work was supported by the Natural Science Foundation Youth Project of Shaanxi Province (No. 2018JQ8014) and the Subject Innovation Team of Shaanxi University of Chinese Medicine (2019-YL06).
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LY, W-lD, and H-jX: designed the experiment, interpreted the data, and prepared the manuscript. B-gZ, YX, X-wW, YF, and LY conducted the experiment, collected the data, and reviewed the manuscript.
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Our study was approved by the ethics review board of the Affiliated Hospital of the Shaanxi University of Traditional Chinese Medicine.
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Yang, L., Deng, Wl., Zhao, Bg. et al. FOXO3-induced lncRNA LOC554202 contributes to hepatocellular carcinoma progression via the miR-485-5p/BSG axis. Cancer Gene Ther 29, 326–340 (2022). https://doi.org/10.1038/s41417-021-00312-w
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DOI: https://doi.org/10.1038/s41417-021-00312-w
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