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CRISPR/Cas9 genome editing technology significantly accelerated herpes simplex virus research

Cancer Gene Therapy volume 25, pages 93–105 (2018)Cite this article

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Abstract

Herpes simplex viruses (HSVs) are important pathogens and ideal for gene therapy due to its large genome size. Previous researches on HSVs were hampered because the technology to construct recombinant HSVs were based on DNA homology-dependent repair (HDR) and plaque assay, which are inefficient, laborious, and time-consuming. Fortunately, clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9 (CRISPR/Cas9) recently provided the possibility to precisely, efficiently, and rapidly edit genomes and indeed is successfully being used in HSVs. Importantly, CRISPR/Cas9 technology increased HSV HDR efficiency exponentially by a 10,000–1,000,000 times when making recombinant HSVs, and its combination with flow cytometric technology made HSV recombination practically automatic. These may have a significant impact on virus and gene therapy researches. This review will summarize the latest development and molecular mechanisms of CRISPR/Cas9 genome editing technology and its recent application in HSVs.

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Acknowledgements

We thank Hubei province for funding HWX as a Chutian Scholar Distinguished Professor, the National Natural Science Foundation of China (31700736 to XWW), Hubei Province Natural Science Foundation of China (2016CFB180 to XWW), and Yangtze University for fellowship to DW as a graduate student.

Funding

This study was funded by 20160527 (Hubei province of China for funding to HWX as a Chutian Scholar Distinguished Professor), the National Natural Science Foundation of China (31700736 to XWW), and Hubei Province Natural Science Foundation of China (2016CFB180 to XWW).

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  1. The Second Clinical Medical School, Yangtze University, 434023, Jingzhou, Hubei Province, China

    Dong Wang, Xian-Wang Wang, Xiao-Chun Peng, Ying Xiang, Shi-Bao Song, Ying-Ying Wang, Zhao-Wu Ma & Hong-Wu Xin

  2. Center for Oncology, Yangtze University Health Science Center, 434023, Jingzhou, Hubei Province, China

    Dong Wang, Xian-Wang Wang, Xiao-Chun Peng, Ying Xiang, Shi-Bao Song, Ying-Ying Wang, Zhao-Wu Ma & Hong-Wu Xin

  3. Department of Biochemistry and Molecular Biology, School of Basic Medicine, Changsha Medical University, 410219, Changsha, Hunan Province, China

    Lin Chen

  4. Montgomery Blair High School, Silver Spring, MD, 20901-2451, USA

    Victoria W. Xin

  5. Institute for Infectious Diseases and Endemic Diseases Prevention and Control, Beijing Center for Diseases Prevention and Control, 100013, Beijing, China

    Yan-Ning Lyu

  6. Department of Gastrointestinal Surgery, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital and Institute, 100142, Beijing, China

    Jiafu Ji

  7. Department of Laboratory Medicine, Jingzhou Central Hospital, the Second Clinical Medical School, Yangtze University, 434023, Jingzhou, Hubei Province, China

    Cheng-Bin Li

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Wang, D., Wang, XW., Peng, XC. et al. CRISPR/Cas9 genome editing technology significantly accelerated herpes simplex virus research. Cancer Gene Ther 25, 93–105 (2018). https://doi.org/10.1038/s41417-018-0016-3

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