Abstract
Background
There is a need to prognosticate the severity of cystic fibrosis (CF) detected by newborn screening (NBS) by early assessment of CF transmembrane conductance regulator (CFTR) protein function. We introduce novel instrumentation and protocol for evaluating CFTR activity as reflected by β-adrenergically stimulated sweat secretion.
Methods
A pixilated image sensor detects sweat rates. Compounds necessary for maximum sweat gland stimulation are applied by iontophoresis, replacing ID injections. Results are compared to a validated β-adrenergic assay that measures sweat secretion by evaporation (evaporimetry).
Results
Ten healthy controls (HC), 6 heterozygous (carriers), 5 with CFTR-related metabolic syndrome (CRMS)/CF screen-positive, inconclusive diagnosis (CFSPID), and 12 CF individuals completed testing. All individuals with minimal and residual function CFTR mutations had low ratios of β-adrenergically stimulated sweat rate to cholinergically stimulated sweat rate (β/chol) as measured by either assay.
Conclusions
β-Adrenergic assays quantitate CFTR dysfunction in the secretory pathway of sweat glands in CF and CRMS/CFSPID populations. This novel image-sensor and iontophoresis protocol detect CFTR function with minimal and residual function and is a feasible test for young children because it is insensible to movement and it decreases the number of injections. It may also assist to distinguish between CF and CRMS/CFSPID diagnosis.
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Introduction
Cystic fibrosis (CF) is a genetically transmitted disease caused by a defective anion channel, CF transmembrane conductance regulator (CFTR). Defective CFTR results in abnormal mucus secretions causing obstruction in the lungs and pancreas, leading to life-threatening lung infections and malnutrition. Thousands of infants in the United States are identified annually through newborn screening (NBS) to be at risk for developing CF and are referred to CF Foundation-accredited centers for confirmation of diagnosis (www.cff.org). The standard sweat chloride test (SCT) uses quantitative pilocarpine iontophoresis to diagnose CF.1 This test is based on the pathophysiology of defective chloride reabsorption in sweat ducts that causes elevated chloride concentrations in sweat from patients with CF2 (Supplemental Fig. S1). The SCT is accepted worldwide as the gold standard diagnostic test for CF; however, it was established with patients with severe disease vs. healthy subjects.3 It assesses CFTR protein function through analysis of the level of chloride content in the sweat collected after 30 min of electrical–chemical stimulation of the skin.1,4 Sweat chloride ≥60 mmol/L confirms the diagnosis; <30 mmol/L is considered negative or “CF unlikely”; and 30–59 mmol/L is considered intermediate.5 Intermediate sweat chloride results (and even negative results with two identified CFTR mutations detected by NBS) represent a significant challenge to physicians when determining prognosis and the appropriate level of management, because those individuals can potentially develop moderate to severe disease and conversely may not develop CF symptoms at all. Treatment is only initiated upon confirmed diagnosis or presentation of symptoms. Currently, positive NBS children with intermediate range will be followed and standard SCT repeated multiple times along the child’s life. Signs and symptoms and confirmed diagnosis have been reported in individuals with negative and intermediate SCT in specific genotypes: D1152H and 3849 + 10kbC>T.4,6,7,8
Since the CF gene was discovered in 1989, >2000 different CFTR variants have been identified (http://genet.sickkids.on.ca), and the disease is now better understood as a spectrum expressing mild to severe phenotypes. A broader use of genetic testing in NBS revealed a subset of subjects genetically screen positive, who are asymptomatic, and have negative or intermediate SCT results. These individuals are given a diagnosis of CFTR-related metabolic syndrome (CRMS) or CF screen-positive, inconclusive diagnosis (CFSPID) and they are at risk to develop disease associated with CFTR protein dysfunction in the future.9 Genotyping is now part of most NBS programs and an important component of confirmation of diagnosis in the United States and in other parts of the world.5,10 The focus on genotyping is justified by the fact that there are three recently approved drugs capable of partially improving function of the defective protein.1,11,12 With this shift in paradigm, the standard SCT has become limited in defining diagnosis. Thus, there is a clear need to develop an assay that can better assess CFTR function in vivo, not only to define diagnosis and prognosis in patients with mild to severe disease but also to define potential responses to CFTR-targeted drugs.
The rate of adrenergically stimulated sweat secretion test provides a unique, essentially non-invasive, physiological approach to determine CFTR function in vivo. Human sweat glands secrete sweat via stimulation of two apparently independent neural pathways: cholinergic and adrenergic. The cholinergic pathway controls thermoregulation and the β-adrenergic pathway is thought to be related to a “fight or flight” response.13 The cholinergic pathway appears to be independent of CFTR, while the β-adrenergic pathway is highly CFTR-dependent.14,15 Consequently, β-adrenergic sweat rates can be used as a physiological marker of CTFR activity (Fig. 1). The initial sweat secretion test was developed in adults by measuring the ratio of the rate of evaporation of sweat with an evaporimeter (cyberDERM Inc.) after sequentially stimulating the two pathways independently. Figure 1 shows how the standard SCT differs from the β-adrenergic sweat secretion test. We applied the evaporimeter technology to test 30 children who were screen positive for CF. Although safe and well tolerated, measurements in infants (n = 5) were inaccurate due to movement artifacts, and in the pre-school age group, the sweat rate response to β-adrenergic stimulation (CFTR function) was not statistically different among those with symptoms and deleterious mutations (CF n = 16) vs. those who were asymptomatic and with benign variants of the gene (CRMS/CFSPID n = 10). Thus, sweat secretion assays as measured by the evaporimeter did not adequately discriminate between verified CF subjects and the CRMS/CFSPID group.16
The present work presents new technology and protocol that determine in vivo CFTR function as reflected by β-adrenergically stimulated eccrine sweat secretion. We hypothesize that this new technology and protocol are minimally invasive, suitable to be applied to young children, and sensitive to detect minimal and residual CFTR function. The technology may be used to develop a clinical test to confirm the diagnosis of CF after a positive NBS, to define prognosis of CF screen-positive infants to personalize care, and/or to quantitate responses to CFTR-targeted therapy in vivo.
Methods
Subjects
The Institutional Review Boards from Children’s Hospital Los Angeles (CHLA) and the University of Southern California (USC) approved the study. Written informed consent was obtained from all subjects ≥18 years old, and from the subjects’ parents for all minors (<18 years old). Assent was obtained from subjects 7–18 years old. Only non-CF healthy controls (HCs) ≥18 years old were recruited during protocol development. HCs were recruited based on self-reporting of no chronic respiratory symptoms and no family history of CF; they did not have SCT or genotyping. CF and CRMS/CFSPID patients were diagnosed based on accepted CF Foundation (CFF) criteria and recruited from CHLA CF center.5 The parents of patients who were previously genotyped were also recruited for testing.
Instrumentation
The measurement is based on the change in local electrical capacitance that results from the generation of sweat by eccrine sweat glands stimulated by cholinergic or adrenergic agonists. The capacitive fingerprint module (HF-EM401, HuiFan Technology Co., China) senses the capacitance on each pixel of the detector to produce an image of the fingerprint ridges and valleys, the air gaps in the valleys having a higher capacitance than the ridges. Sweat production decreases the local capacitance and modifies the image. A thin piece of filter paper placed on the sensor masks the texture of the skin surface. The resulting image is generated as sweat wets the paper non-uniformly as a function of local sweat secretion from individual glands.
To conduct the sweat test, the capacitive sensor module is positioned on the ventral surface of the forearm with a 3D-printed holder that maintains the module in tight contact with the skin with a Velcro strap wrapped around the forearm. Since the image intensity varies with the pressure with which the module is held on the skin, a resistive pressure sensor (FSR 400, Interlink Electronics, Westlake Village, CA) was installed behind the fingerprint module. A cushion of stiff plastic foam was used to normalize the contact pressure between the skin and the sensor module and standardize the contact pressure of the sensor module on all subjects (Fig. 2).
Two types of filter paper (GE Healthcare, Pittsburgh, PA) were used: (1) Whatman AE 99 (120 μm thickness, 8 μm pore size) was used to measure sweat production after cholinergic stimulation. (2) Whatman Cyclopore polycarbonate membrane (7 μm thickness, 0.1 μm pore size) was used after adrenergic stimulation. Two different filter papers were used because the thinner Cyclopore paper allowed detection of small amounts of sweat observed in CF subjects after adrenergic stimulation. However, the thinner paper saturated quickly with the larger amounts of sweat observed in HCs after cholinergic stimulation and therefore required thicker paper.
Image acquisition and analysis
Custom software written in LabVIEW (National Instruments, Austin, TX) was used to transfer the sensor image every 5 s from the fingerprint module controller to the computer using serial communication. The gray-scale images (200 × 153 pixels) coded on 8 bits were displayed on the computer monitor to visualize the production and accumulation of sweat on the sensor surface. Analysis of the image sequence during a sweat stimulation protocol and evaluation of the sweat rate was carried out as shown in Fig. 3.
Study protocol
Protocol development
Iontophoresis of pilocarpine 1%
Iontophoresis was performed to decrease the number of intradermal (ID) injections. Initial measurements were performed comparing the standard Pilogel 0.5% (ELITechGroup) and pilocarpine 1% (USC Compounding pharmacy). Maximum sweat rate was measured by a evaporimeter (Model RG1, cyberDERM Inc., Broomall, PA). The subject was seated comfortably with both arms resting on a table. A small area of skin on the ventral surface of the forearms (about 4 × 4 cm2) was gently cleaned with distilled water, dried, and covered by a thin layer of mineral oil to minimize transepidermic water loss. Evaporimeter probes were placed on these cleaned areas and secured with Velcro bands. Baseline activity was measured for 5 min. Sweat secretion was then stimulated by iontophoresis of Pilogel® on one arm and subsequently by pilocarpine 1% on the opposite arm. Circular-shaped gels (Pilogel®) or circular-shaped felt patches soaked in pilocarpine 1% solution were mounted on the sweat-inducer iontophoresis discs (Model 3700, Wescor Inc., Logan, UT), which applies 1.5 mA current for 5 min between electrodes to iontophorese drugs transcutaneously into the ID space (Fig. 2).
ID injections compared to iontophoresis
Quinton et al.14 validated the evaporimeter protocol using subsequent ID injections of carbachol, atropine, and a β-adrenergic cocktail. Serial injections were well tolerated by adults in the validation study, but would be less manageable in a pediatric population. We then compared ID carbachol 0.1 ml = 0.01 µg (Miostat intraocular, Alcon®) and iontophoresed pilocarpine 1% measured by evaporimeter in a similar fashion as described for Pilogel 0.5% and pilocarpine 1% experiments. Similarly, ID atropine 0.2 ml = 8.8 μg (Bausch & Lomb) and atropine 5% (USC Compounding Pharmacy) were compared. Several attempts were made to iontophorese the β-adrenergic cocktail; however, sweat rate results were not reproducible when atropine, aminophylline, and isoproterenol were iontophoresed concurrently or sequentially.
Sweat gland potentiation
We evaluated the use of the ID β-adrenergic cocktail in HCs (n = 2) with and without prior “sweat gland potentiation” with ID carbachol followed by ID atropine measured by both methods.17
Reproducibility of image sensor
Reproducibility of the sweat response measured by the image sensor was assessed in three HCs who received three ID carbachol at neighboring sites on the ventral surface of the arm. We also map the darkened spots visualized in response to pilocarpine and β-adrenergic cocktail to demonstrate that the same sweat glands were stimulated.
Final protocol
In the final protocol, sweat rate was measured on both arms concurrently (Fig. 2). The subject was seated and skin preparation of the forearms was as described above. Evaporimeter was applied to the right arm and the image sensor to the left arm. Baseline secretion was measured for 5 min. Sweat secretion was then stimulated simultaneously on both arms by iontophoresing pilocarpine 1%, followed by Atropine 5%. The skin was marked with a pen to reposition the probes in the same site. The effect of blocking cholinergic response with atropine was confirmed by the evaporimeter only on the right arm. Once the evaporimeter signal stabilized (usually 2–5 evaporimeter units above baseline), an ID injection of 0.2 ml β-adrenergic cocktail was administered containing: 8.8 μg of atropine, 940 μg of aminophylline (Regent Laboratories Inc.), and 8 μg of isoproterenol (Hospira Inc.). After injecting both sides, the evaporimeter and the image-sensor probes were repositioned over the sites of ID injections on the right and left arms, respectively.
Statistical analysis
Patient characteristics and distribution of maximum sweat rate were described by summary statistics. Mean and standard deviation (SD) were used to describe the variables with a normal distribution, while median and interquartile range was used for the variables with non-normally distributed. Frequency and percentages were used to summarize categorical variables. Maximum sweat rate measurements were natural log-transformed to appropriately adjusting for the non-normal distribution and analysis of variance was used to examine the differences in these measurements for both evaporimeter and image-sensor assays across HCs, carriers, CRMS/CFSPID, and CF groups. Then, multiple comparisons with Tukey’s adjustment was used to further assess which of the pairwise comparisons differ in maximum sweat rate measurements. Taking into account that the two methods have different units, z-scores were calculated based on total sample mean for each method in order to assess β/chol ratio difference for all groups. The difference in the z-scores between two methods was assessed by repeated-measure analysis of variance. Statistical significance was set at 5% level with two-tailed test throughout the analyses. All statistical computations were done in Stata/SE 15.1 (StataCorp, College Station, TX).
Results
Initial results during protocol development
Iontophoresis of pilocarpine 1% and Pilogel 0.5% were equivalent
Iontophoresis of Pilogel 0.5% and pilocarpine 1% on the same HC individuals were equivalent (104 ± 7.4 and 104 ± 13.4 mg H2O/m2/h, respectively, n = 5; p = 0.986).
ID injections compared to iontophoresis
ID carbachol 0.1 ml = 0.01 µg and iontophoresed pilocarpine 1% measured by evaporimeter had similar results: 84 ± 11.5 and 82 ± 12.7 mg H2O/m2/h (p = 0.49, HCs n = 33, CF n = 13). atropine-injected vs. iontophoresed blocked 95 and 83%, respectively, of the cholinergic sweat rate (n = 11, p = 0.345).
Sweat gland potentiation with cholinergic stimulation is necessary for maximum β-adrenergic stimulated rate
When evaluating the use of the ID β-adrenergic cocktail in HC (n = 2) with and without prior “sweat gland potentiation,” maximum sweat rate in response to adrenergic stimulation without potentiation was about half the rate with potentiation, when measured by both evaporimeter and image sensor: evaporimeter—no potentiation: 40 (36–44) median (95% confidence interval (CI)) and with potentiation: 77 (66–83) (p = 0.004). Image sensor—no potentiation: 36 (33–38) and with potentiation: 65 (49–112) (p = 0.001), confirming previous findings.17,18
Sweat rates measured by the image sensor were reproducible
Sweat rates after injection of carbachol in three different locations on the volar aspect of the arm of three individual subjects showed: subject 1 = 102.57 ± 1.89, subject 2 = 69.66 ± 16.60, and subject 3 = 184.03 ± 19.03 intensity/time/mm2. The sequence of sensor images gathered after stimulation of the sweat glands demonstrated the production and gradual accumulation of sweat at the stimulated site. Mapping the darkened spots visualized in response to pilocarpine and β-adrenergic cocktail indicated that the same sweat glands were measured with each repeated stimulation (Fig. 4).
Evaporimeter and image-sensor measurements were comparable
A total of 10 HC, 6 heterozygous (carriers), 5 CRMS/CFSPID, and 12 CF individuals completed testing using the final protocol (Table 1). Group characteristics were as follows: age in years (SD): HC = 32 (13), carrier = 38 (8), CRMS/CFSPID = 8 (1), and CF = 16 (4); race/ethnicity: HC = 100% white/10% Hispanic, carrier = 100% white/67% Hispanic and 33% Caucasian, CRMS/CFSPID = 100% white/60% Hispanic and 40% Caucasian, and CF = 83% white and 17% black/33% Hispanic, 50% Caucasian, and 17% African descent. CFTR mutations/variants were identified by NBS and parent DNA testing. Evaporimeter and image-sensor measurements had a linear relationship when quantifying maximum sweat rates in response to β-adrenergic stimulation in HC and CF (Spearman’s correlation = 0.8379, Supplemental Fig. S2). There is no significant difference in the sweat rate measurements between evaporimetry and image sensor according to z-scores analyses (Table 2).
Minimal and residual function CFTR mutations yield low β-adrenergic/cholinergic sweat rate ratios
Table 1 shows individual β/chol ratios measured by both assays. Figure 5 shows individual image-sensor-β/chol ratio plotting and genotype with corresponding CFTR I–VI classes and functional classification. All CRMS/CFSPID subjects had one or both CFTR mutations classified as residual function as did 2 of the 12 CF subjects (siblings with the same genotype: 3849 + 10kbC>T/935delA). All minimal and residual function CFTR mutation combinations had low β/chol ratios when measured by both assays.
β-Adrenergic/cholinergic ratio in natural log from evaporimetry and image-sensor measurements were able to differentiate between CRMS/CFSPID and CF
β/Chol ratio (after natural log transformed) from evaporimetry and image-sensor measurements were equally successful to show significant differences between HC and CRMS/CFSPID, HC and CF, carrier and CRMS/CFSPID, carrier and CF, and CRMS/CFSPID and CF (Fig. 6).
Discussion
The image-sensor and Iontophoresis protocol presented here illustrate initial development of a novel application of technology to detect minimal and residual CFTR function in vivo. The image-sensor assay detects sweat rates in response to β-adrenergic stimulation in individuals diagnosed with CF and CRMS/CFSPID that can be performed on pediatric subjects. As seen previously with the evaporimeter, both groups had low β-adrenergic sweat rate,16 which suggests CFTR dysfunction in CFTR-dependent sweat secretion in glands of both CF and CRMS/CFSPID children. The advantages of this technology and proposed iontophoresis protocol over other proposed assays are that: (a) it can be completed in <30min, (b) it requires only one ID injection, as iontophoresis of pilocarpine and atropine are applied prior to the β-cocktail injection; (c) the apparatus is insensitive to subject movement, making it more feasible for use in a younger population; and (d) it detects β-induced sweat secretion over the full range of CFTR function, which may assist in differentiating between CF and CRMS/CFSPID diagnosis.
Since the mid-1980s when Sato and Sato15,18 discovered that CF glands do not secrete sweat in response to β-adrenergic stimuli, but secrete normally after exposure to cholinergic secretagogues, there have been several attempts to develop an assay with detection limits that are low enough to capture differences in β-adrenergic sweat rate responses among CF subjects with different CFTR mutations.17 A ratiometric assay comparing β-adrenergic-to- cholinergic sweat rates using the evaporimeter was validated several years ago for differentiating heterozygotes from CF genotypes.14 However, evaporimetry was not sufficiently sensitive to differentiate among CF subjects with different levels of disease severity such as pancreatic sufficient vs. insufficient subjects. As there was a significant difference between CFTR-related disorder (which is a single organ disease caused by two CFTR mutations) and CF, our group attempted to apply evaporimetry to determine differences between CF and CRMS/CFSPID.16 To our surprise, the CFTR function in CRMS/CFSPID assayed by this method appeared just as low as in CF individuals. The current image-sensor protocol shows no significant difference with the evaporimeter measurements, but with a slight advantage of a lower detection limit for the CFTR-related response. The image sensor detected β-adrenergic sweat rate responses in two individuals who had zero response (consequently zero ratios) when measured by the evaporimeter (genotypes: G330X/G480C and F508del/W1282X). Since these genotypes are known to express very little protein, these results may be an artifact and not the actual β-adrenergic sweat secretion. However, in view of the cautious phases of protocol development including reproducibility and mapping of the sweat glands, this possibility seems unlikely. The β-adrenergic-induced sweat secretion was low regardless of genotype, CFTR class, or category of residual/minimal function (Fig. 5). Yet, the image sensor detected some level of β-adrenergic sweat rate responses in 100% of CF subjects, while the evaporimeter detected sweat rate in 80% of subjects with residual/minimal CFTR function (Table 1).
An optical ratiometric technology assay also comparing β-adrenergic-to-cholinergic sweat rates has been developed to measure CFTR function in persons with CF-pancreatic sufficiency/insufficiency and with CFTR-related disorder.17,19 This assay was used to measure the effect of a CFTR modulator (Ivacaftor) on single, individual sweat glands in subjects carrying G551D and R117H-5T.17,20 β-Adrenergic response was seen in 16–21% of sweat glands of individuals treated with the drug and in no glands in subjects on placebo. This finding suggested that the optical ratiometric method is more sensitive than the evaporimeter to measure sweat rate response to CFTR-targeted therapy.21 Although highly sensitive, the optical ratiometric assay does not detect β-adrenergic-induced sweat secretion in individuals carrying CFTR mutations classified as minimal function and in others with CFTR-related disorder measurements were highly variable (0–0.6).17,19 Additionally, it requires maintaining the same position without any movement for a prolonged period of time along with several ID injections, making it less suitable for children.
The image sensor and the evaporimeter were equally successful to differentiate between groups (HC, carrier, CRMS/CFSPID, and CF). Interestingly, the natural log of β/chol ratios showed significant difference between CRMS/CFSPID and CF, suggesting that both methods and this iontophoresis protocol may complement standard SCT in defining the diagnosis of screen-positive children carrying CFTR mutations/variants of unknown clinical significance. Another possible application in this NBS population would be to use this assay to monitor CRMS/CFSPID to CF reclassification over time. The current findings are different than our previous work, which showed no difference between CF and CRMS/CFSPID pre-school age children when sweat rate was measured by evaporimetry after carbachol and β-cocktail injections given on different days. The difference may be explained by several factors: (1) different protocol (sweat glands were not potentiated); (2) different age groups (sweat glands may not be fully developed in 4–6-year-old children); and (3) small samples in both studies. Also, in our experiments the evaporimeter (or the image sensor) did not detect a significant difference between HC and heterozygous (carriers) as previously seen by other groups.14,17,19 Thus, it is important to plan for a larger study using this new technology and iontophoresis protocol, including all four groups.
The present protocol is better tolerated because it is less invasive, as it uses 1 vs. 3 ID injections. The iontophoresis of pilocarpine and atropine was developed after our group repeated and confirmed that pre-stimulation of the sweat gland with cholinergic drugs potentiates β-adrenergic-induced sweat secretion.17 Ideally, we would eliminate all injections, but iontophoresis of β cocktail were not reproducible when testing the same individual on the same or different days. A possible explanation is the fact that the three drugs in the cocktail (atropine, aminophylline, and isoproterenol) are not pharmacologically compatible. These compounds have different charges, pH, and aminophylline accelerates degradation of isoproterenol. As in previous protocols β cocktail drugs were mixed immediately before injecting or iontophoresing so that the drug effect would not be compromised. For iontophoresis more promising results were achieved when drugs were iontophoresed sequentially. However, three sequential iontophoresis prolonged the protocol to about one hour and caused skin irritation, which created measurement artifacts and was judged to be more uncomfortable than injections.
Limitations to this work include: (1) Small sample size that completed the final protocol, which can be justified by the fact that this is an introduction to this new technology and protocol; validation studies will require bigger samples (precisely n = 15 in each of the four groups for 80% power). On the other hand, within the CRMS/CFSPID group we recruited a broad representation of genotypes: from individuals carrying benign variants (F508del and (TG)11–5T) to others carrying variants of varying clinical consequence (F508del and D1152H).22,23 (2) The image sensor does not yield results expressed in physical units. To accommodate the different units, we used z-scores to compare the two methods. (3) There was no direct comparison to the standard SCT. We considered this step irrelevant to the proof of concept of this protocol as these two assays measure different functions of CFTR in the sweat gland (secretory vs. absorptive).
In conclusion, the β-adrenergic assays remain as a valid method to complement the standard SCT, since CFTR secretory rather than absorptive function is assayed. The advantages of the image-sensor and this iontophoresis protocol over previously proposed assays are the detection of CFTR function with minimal and residual function and its practical utility for measurement in young children. The fact that both image-sensor and the evaporimeter assays detect β-adrenergic sweat in CRMS/CFSPID almost as low as in CF suggests CFTR dysfunction in the secretory pathway of sweat glands in both populations. The β/chol ratio (after natural log transform) successfully differentiates between CRMS/CFSPID and CF groups, when measured by both evaporimetry and image sensor. Once these findings are validated in a larger trial and by other groups, the image-sensor assay and iontophoresis protocol can assist in defining CF and CRMS/CFSPID diagnosis after a positive NBS and monitoring for CRMS/CFSPID to CF reclassification over time. Future directions include testing in a larger sample of young children from NBS, and also before and after use of CFTR-targeted drugs.
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Acknowledgements
Paul Quinton, Ph.D. (University of California, San Diego), for outstanding mentorship, conceptual support, and critical revision of the article for important intellectual content; Dennis Briscoe (Ellitech) for conceptual and technical support; and Lilit Grigoryan, Nadine Afari, Michael Bauschard, Alexandra Franquez, Alex Katz and Dipayon Roy for their support in recruiting, coordinating, and executing the experiments. This project received funding from NIH/NCRR/NCATS: Grant KL2TR000131; SC-CTSI Pilot Grant 8UL1TR000130; and Cystic Fibrosis Research Incorporated (CFRI Grant# Salinas15-SC01).
Authors contributions
D.B.S., J.-M.M., Y.-H.P., B.H., and C.P.W. had full access to all the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis. Study concept and design: D.B.S, J.-M.M., Y.-H.P., B.H. and E.F.; data acquisition and analysis: D.B.S., Y.-H.P., B.H. and J.-M.M.; interpretation: D.B.S., J.-M.M., Y.-H.P., B.H. and E.F.; drafting of the article: D.B.S., Y.-H.P., B.H., J.-M.M. and C.P.W.; critical revision of the article for important intellectual content: D.B.S., Y.-H.P., B.H., C.P.W. and J.-M.M.; obtained funding: D.B.S., Y.-H.P. C.P.W. and J.-M.M.; statistical analysis: C.P.W.
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Salinas, D.B., Peng, YH., Horwich, B. et al. Image-based β-adrenergic sweat rate assay captures minimal cystic fibrosis transmembrane conductance regulator function. Pediatr Res 87, 137–145 (2020). https://doi.org/10.1038/s41390-019-0503-8
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DOI: https://doi.org/10.1038/s41390-019-0503-8
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