To the Editor:
Patients with polycythemia vera (PV), an incurable Philadelphia-negative myeloproliferative neoplasm, have shortened survival due to thrombohemorrhagic complications and evolution to myelofibrosis or acute myeloid leukemia [1, 2]. Clinical management of PV has primarily addressed the thrombotic risk, whereas prevention of disease progression remains a significant unmet need [3]. Interferon alfa is recognized as a disease-modifying treatment for PV that may reduce the risk of myelofibrotic progression and potentially prolong overall survival according to retrospective data, but prospective studies to confirm these findings have been lacking [4]. Final results from the large PROUD-PV trial and its extension CONTINUATION-PV are now available comparing ropeginterferon alfa-2b (BESREMi®) with hydroxyurea or best available treatment (BAT) over a total of 6 years.
PROUD-PV (#NCT01949805) was a phase three, randomized, controlled, open-label trial conducted in 48 centers in Europe enrolling patients diagnosed with PV (WHO 2008 criteria), who regardless of conventional risk status required cytoreduction, and were hydroxyurea-naïve or pre-treated for <3 years [5, 6]. Patients gave informed consent and were randomized 1:1 (stratified by age, thrombotic history, and hydroxyurea pretreatment) to receive ropeginterferon alfa-2b or control treatment (hydroxyurea). Dosing was escalated until blood counts normalized (ropeginterferon alfa-2b: from 50–100 µg to a maximum of 500 µg every two weeks; hydroxyurea: from 500 mg to 3000 mg daily). After one year, patients could roll over into CONTINUATION-PV (#NCT02218047) for 5 further years of treatment. Patients allocated to the control arm could switch to any standard cytoreductive therapy (i.e. BAT), those in the ropeginterferon alfa-2b arm could extend the dosing interval to three or four weeks. Detailed design and methodology have been published elsewhere [5, 6].
Efficacy outcomes over 6 years were analyzed in the full analysis set for CONTINUATION-PV; all safety data were evaluated regardless of inclusion in the extension study. Time to first risk event (thromboembolic events, disease progression or death [all causes]) was evaluated by Kaplan-Meier analyses and the groups compared by log-rank test and by Cox proportional hazards model. Complete hematologic response (CHR) defined according to modified European LeukemiaNet (ELN) criteria [5] was compared between the groups using a log-binomial regression model; rate ratio (RR) and 95% confidence intervals (CI) were calculated from estimates of regression coefficients. Discontinued patients were considered non-responders for CHR in the primary analysis; a sensitivity analysis utilizing imputation of the last observation carried forward (LOCF) was conducted. Comparison of molecular response defined by ELN criteria [7] between the arms employed the same method as for CHR. LOCF is reported for all molecular analyses. AEs were analyzed descriptively.
In PROUD-PV, 127 patients were treated in each arm of whom 95 in the ropeginterferon alfa-2b arm and 74 in the control arm rolled over into CONTINUATION-PV (Fig. S1). Baseline characteristics (at screening in PROUD-PV) in the extension study population were comparable between the study arms regarding age, time since diagnosis, JAK2V617F allele burden, non-driver mutations, spleen size, presence of PV-related symptoms and the history of thromboembolic events and cardiovascular risk factors.
Individualized dose-titration of ropeginterferon alfa-2b resulted in a median cumulative 4-weekly dose in the 6th year of treatment of 499 µg (IQR: ±268–782 µg), administered at an extended 3- or 4-week interval in most patients (61.9%). In the control arm, 87.8% of patients remained on hydroxyurea as of the 72-month assessment with a median dose of 1000 mg/day (IQR: 750–1500 mg). The study ended on 29 April, 2021 when all patients had been treated for ≥6 years; the maximum duration was 7.3 years. Cumulative exposure was 568 patient-years (ropeginterferon alfa-2b) and 451 patient-years (control treatment). A total of 67/95 patients in the ropeginterferon alfa-2b arm and 52/74 in the control arm completed the study, giving rise to equivalent discontinuation rates.
Clinical events during PROUD-PV/CONTINUATION-PV were rare; median event-free survival was not reached (Fig. 1). The probability of event-free survival was significantly higher in the ropeginterferon alfa-2b arm compared with the control treatment group (0.94 versus 0.82; log-rank test; p = 0.04) as shown by analysis of the time to first risk event. The Cox proportional hazard ratio was 0.34 (95% CI: 0.12–0.97; p = 0.04). Risk events occurred in 5/95 patients (5.3%) in the ropeginterferon alfa-2b arm (first events: thromboembolic events [n = 2]; myelofibrosis [n = 1]; death [n = 2]) compared with 12/74 patients (16.2%) allocated to hydroxyurea/BAT (thrombotic events [n = 5]; myelofibrosis [n = 2]; acute leukemia [n = 2]; death [n = 3]).
These results align with potentially improved survival in interferon-treated PV patients compared with hydroxyurea treatment or phlebotomy in prior observational studies [4, 8]. Supporting a treatment effect in the current prospective study, risk factors associated with a poor prognosis in PV were balanced between the treatment arms at study start, largely due to stratification during randomization. Furthermore, no selection bias due to the roll-over was detected in analyses of patient characteristics and response rates comparing patients who enrolled or did not enroll into CONTINUATION-PV [5]. The final results of PROUD-PV/CONTINUATION-PV therefore indicate that the disease-modifying potential of ropeginterferon alfa-2b suggested by prior analyses [6] can now be quantified using hard clinical endpoints.
JAK2V617F allele burden is utilized as a surrogate endpoint for disease modification due to its central role in the pathophysiology of PV, and recent findings substantiate the notion that molecular response to treatment can alter the natural course of the disease [9].
Ropeginterferon alfa-2b profoundly diminished the JAK2V617F allele burden long-term: after 6 years, 62/94 patients (66.0%) achieved a molecular response (ELN criteria) compared with 14/72 (19.4%) in the control arm (risk ratio: 3.23 [2.01 to 5.19]; p < 0.0001). In line with our data published previously [6], median JAK2V617F allele burden at Month 72 was 8.5% versus 50.4% in the ropeginterferon alfa-2b and control arms, respectively (p < 0.0001) (Fig. S2). Moreover, allele burden >50%, which is presumed to reflect homozygosity for the JAK2V617F mutation and is associated with increased risks of thrombosis and disease progression [9,10,11], was found in only 11.6% of patients in the ropeginterferon alfa-2b arm in the 6th year of treatment compared with 50.0% of patients receiving hydroxyurea/BAT (p < 0.0001).
Regarding hematologic parameters conventionally used to determine therapeutic efficacy, the significantly higher CHR rate in the ropeginterferon alfa-2b arm compared with the control arm reported at 3 years persisted after 6 years of treatment (month 72: 48/88 [54.5%] versus 22/63 [34.9%]; RR:1.55 [95% CI: 1.07 to 2.26; p = 0.02) [5]. A sensitivity analysis utilizing imputation of LOCF confirmed the higher response rate in the ropeginterferon alfa-2b arm (69/95 [72.6%] versus 35/74 [47.3%]; RR: 1.54 (95% CI: 1.18 to 2.00); p = 0.001) (Fig. S3). Despite the more gradual onset of CHR to ropeginterferon alfa-2b compared with control treatment (mainly hydroxyurea), patients treated with ropeginterferon alfa-2b spent a higher proportion of time in CHR than control treated patients (mean: 60.9% versus 41.2%, respectively; p = 0.04), underpinned by less fluctuation in responses according to ELN-defined targets for hematocrit, leukocyte count and platelet count (Figs. 2, S4).
While normalization of blood counts principally serves to minimize thrombotic risk in PV, leukocytosis has also been identified as a biomarker for disease evolution and overall survival [2, 12]. Longitudinal analysis of leukocytosis is essential, since a persistently elevated leukocyte trajectory is significantly associated with increased risk of disease progression (p = 0.0002) [12]. Patients receiving ropeginterferon alfa-2b in the current studies not only achieved a greater reduction in leukocyte count compared to baseline versus control (mean absolute changes: −6.67 × 109/L versus −3.59 × 109/L respectively [p < 0.0001] at Month 72), but spent significantly more time with normal leukocyte counts compared with hydroxyurea/BAT treated patients (mean proportion of time in response: 93.7% vs 80.9%; p = 0.02).
Final safety data from up to 7.3 years’ treatment in PROUD-PV and CONTINUATION-PV confirm the established positive safety profile of ropeginterferon alfa-2b [5, 6]. Only 14/127 (11.0%) of patients in the ropeginterferon alfa-2b arm and 3/127 (2.4%) in the hydroxyurea/BAT arm discontinued due to drug-related toxicity; the higher discontinuation rate in the ropeginterferon alfa-2b arm may reflect the intensive monitoring of certain interferon-alfa class effects, which mandated drug withdrawal according to the protocol. AEs were predominantly of mild or moderate severity, with comparable rates of grade ≥3 treatment-related AEs between the groups over the entire period (ropeginterferon alfa-2b: 15.7%; control: 16.5%). Furthermore, PV-associated AEs declined during ropeginterferon alfa-2b treatment, occurring in 7.1% of patients in the 6th year compared with 12.1% in the control arm.
Normal life expectancy may be achievable in PV based on comparable overall survival in interferon-treated patients and a matched US population (p = 0.3), contrasting with shortened survival (p = 0.03) in non-interferon treated patients at the same academic center [3]. Although overall survival was not evaluated, our findings lend support to this view, providing the first evidence that the durable hematologic and molecular responses observed with long-term ropeginterferon alfa-2b therapy are accompanied by improved event-free survival. This potential advantage should be considered when evaluating the individual risk-benefit relationship for ropeginterferon alfa-2b treatment in patients with PV.
Data availability
The datasets generated during and/or analyzed during the current study are not publicly available to protect patient anonymity in this rare disease population but are available from the corresponding author on reasonable request.
References
Cuthbert D, Stein BL. Polycythemia vera-associated complications: pathogenesis, clinical manifestations, and effects on outcomes. J Blood Med. 2019;10:359–71.
Tefferi A, Rumi E, Finazzi G, Gisslinger H, Vannucchi AM, Rodeghiero F, et al. Survival and prognosis among 1545 patients with contemporary polycythemia vera: an international study. Leukemia. 2013;27:1874–81.
Abu-Zeinah G, Silver RT, Abu-Zeinah K, Scandura JM. Normal life expectancy for polycythemia vera (PV) patients is possible. Leukemia. 2022;36:569–72.
Abu-Zeinah G, Krichevsky S, Cruz T, Hoberman G, Jaber D, Savage N, et al. Interferon-alpha for treating polycythemia vera yields improved myelofibrosis-free and overall survival. Leukemia. 2021;35:2592–601.
Gisslinger H, Klade C, Georgiev P, Krochmalczyk D, Gercheva-Kyuchukova L, Egyed M, et al. Ropeginterferon alfa-2b versus standard therapy for polycythaemia vera (PROUD-PV and CONTINUATION-PV): a randomised, non-inferiority, phase 3 trial and its extension study. Lancet Haematol. 2020;7:e196–208.
Kiladjian J-J, Klade C, Georgiev P, Krochmalczyk D, Gercheva-Kyuchukova L, Egyed M, et al. Long-term outcomes of polycythemia vera patients treated with ropeginterferon Alfa-2b. Leukemia. 2022;36:1408–11.
Barosi G, Birgegard G, Finazzi G, Griesshammer M, Harrison C, Hasselbalch HC, et al. Response criteria for essential thrombocythemia and polycythemia vera: result of a European LeukemiaNet consensus conference. Blood. 2009;113:4829–33.
Crisà E, Cerrano M, Beggiato E, Benevolo G, Lanzarone G, Manzini PM, et al. Can pegylated interferon improve the outcome of polycythemia vera patients? J Hematol Oncol. 2017;10:15.
Moliterno AR, Kaizer H, Reeves BN. JAK2V617F allele burden in polycythemia vera: burden of proof. Blood. 2023;141:1934–42.
Passamonti F, Rumi E, Pietra D, Elena C, Boveri E, Arcaini L, et al. A prospective study of 338 patients with polycythemia vera: the impact of JAK2 (V617F) allele burden and leukocytosis on fibrotic or leukemic disease transformation and vascular complications. Leukemia. 2010;24:1574–9.
Guglielmelli P, Loscocco GG, Mannarelli C, Rossi E, Mannelli F, Ramundo F, et al. JAK2V617F variant allele frequency >50% identifies patients with polycythemia vera at high risk for venous thrombosis. Blood Cancer J. 2021;11:199.
Ronner L, Podoltsev N, Gotlib J, Heaney ML, Kuykendall AT, O’Connell C, et al. Persistent leukocytosis in polycythemia vera is associated with disease evolution but not thrombosis. Blood. 2020;135:1696–703.
Acknowledgements
This study was supported by research funding from AOP Health. Special thanks to the patients and their families, to study support staff. Statement of prior presentation: Presented in part in abstract form at Congresses of the European Hematology Association, 9–17 June 2022 and 8–11 June 2023.
Author information
Authors and Affiliations
Consortia
Contributions
HG, CK, KK, and JJK contributed to the study design, analysis, interpretation of data and preparation of the manuscript. VE contributed to the analysis and interpretation of data and wrote the manuscript. PG, DK, LGK, ME, VR, PD, AI, HP, LS, JM, VY, and JJK and the PROUD-PV Study Group collected clinical data. RK provided critical analytical tools, oversaw analyses and interpreted the data. HH contributed to interpretation of the data and preparation of the manuscript. All authors had full access to the primary study data and reviewed the manuscript.
Corresponding author
Ethics declarations
Competing interests
HG reports grants and personal fees from AOP Health and Novartis, and personal fees from PharmaEssentia and BMS-Pharma. JM reports grants from AOP Health. RK reports personal fees from AOP Health, PharmaEssentia, Qiagen and Novartis and stock ownership in MyeloPro Diagnostics and Research. HH reports Data Monitoring Board honoraria from AOP Health and grants from Novartis. JJK reports grants and personal fees from AOP Health, and personal fees from Novartis, BMS/Celgene, AbbVie and PharmaEssentia. CK, KK, and VE report that they are employees of AOP Health. The remaining authors declare no competing financial interests.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Gisslinger, H., Klade, C., Georgiev, P. et al. Event-free survival in patients with polycythemia vera treated with ropeginterferon alfa-2b versus best available treatment. Leukemia 37, 2129–2132 (2023). https://doi.org/10.1038/s41375-023-02008-6
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41375-023-02008-6
This article is cited by
-
Association of JAK2V617F allele burden and clinical correlates in polycythemia vera: a systematic review and meta-analysis
Annals of Hematology (2024)
-
ROP-ET: a prospective phase III trial investigating the efficacy and safety of ropeginterferon alfa-2b in essential thrombocythemia patients with limited treatment options
Annals of Hematology (2024)