Event-free survival in patients with polycythemia vera treated with ropeginterferon alfa-2b versus best available treatment

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TO THE EDITOR:
Patients with polycythemia vera (PV), an incurable Philadelphianegative myeloproliferative neoplasm, have shortened survival due to thrombohemorrhagic complications and evolution to myelofibrosis or acute myeloid leukemia [1,2].Clinical management of PV has primarily addressed the thrombotic risk, whereas prevention of disease progression remains a significant unmet need [3].Interferon alfa is recognized as a disease-modifying treatment for PV that may reduce the risk of myelofibrotic progression and potentially prolong overall survival according to retrospective data, but prospective studies to confirm these findings have been lacking [4].Final results from the large PROUD-PV trial and its extension CONTINUATION-PV are now available comparing ropeginterferon alfa-2b (BESREMi®) with hydroxyurea or best available treatment (BAT) over a total of 6 years.
PROUD-PV (#NCT01949805) was a phase three, randomized, controlled, open-label trial conducted in 48 centers in Europe enrolling patients diagnosed with PV (WHO 2008 criteria), who regardless of conventional risk status required cytoreduction, and were hydroxyurea-naïve or pre-treated for <3 years [5,6].Patients gave informed consent and were randomized 1:1 (stratified by age, thrombotic history, and hydroxyurea pretreatment) to receive ropeginterferon alfa-2b or control treatment (hydroxyurea).Dosing was escalated until blood counts normalized (ropeginterferon alfa-2b: from 50-100 µg to a maximum of 500 µg every two weeks; hydroxyurea: from 500 mg to 3000 mg daily).After one year, patients could roll over into CONTINUATION-PV (#NCT02218047) for 5 further years of treatment.Patients allocated to the control arm could switch to any standard cytoreductive therapy (i.e.BAT), those in the ropeginterferon alfa-2b arm could extend the dosing interval to three or four weeks.Detailed design and methodology have been published elsewhere [5,6].
Efficacy outcomes over 6 years were analyzed in the full analysis set for CONTINUATION-PV; all safety data were evaluated regardless of inclusion in the extension study.Time to first risk event (thromboembolic events, disease progression or death [all causes]) was evaluated by Kaplan-Meier analyses and the groups compared by log-rank test and by Cox proportional hazards model.Complete hematologic response (CHR) defined according to modified European LeukemiaNet (ELN) criteria [5] was compared between the groups using a log-binomial regression model; rate ratio (RR) and 95% confidence intervals (CI) were calculated from estimates of regression coefficients.Discontinued patients were considered nonresponders for CHR in the primary analysis; a sensitivity analysis utilizing imputation of the last observation carried forward (LOCF) was conducted.Comparison of molecular response defined by ELN criteria [7] between the arms employed the same method as for CHR.LOCF is reported for all molecular analyses.AEs were analyzed descriptively.
In PROUD-PV, 127 patients were treated in each arm of whom 95 in the ropeginterferon alfa-2b arm and 74 in the control arm rolled over into CONTINUATION-PV (Fig. S1).Baseline characteristics (at screening in PROUD-PV) in the extension study population were comparable between the study arms regarding age, time since diagnosis, JAK2V617F allele burden, non-driver mutations, spleen size, presence of PV-related symptoms and the history of thromboembolic events and cardiovascular risk factors.
Individualized dose-titration of ropeginterferon alfa-2b resulted in a median cumulative 4-weekly dose in the 6th year of treatment of 499 µg (IQR: ±268-782 µg), administered at an extended 3-or 4-week interval in most patients (61.9%).In the control arm, 87.8% of patients remained on hydroxyurea as of the 72-month assessment with a median dose of 1000 mg/day (IQR: 750-1500 mg).The study ended on 29 April, 2021 when all patients had been treated for ≥6 years; the maximum duration was 7.3 years.Cumulative exposure was 568 patient-years (ropeginterferon alfa-2b) and 451 patientyears (control treatment).A total of 67/95 patients in the ropeginterferon alfa-2b arm and 52/74 in the control arm completed the study, giving rise to equivalent discontinuation rates.
Clinical events during PROUD-PV/CONTINUATION-PV were rare; median event-free survival was not reached (Fig. 1).The probability of event-free survival was significantly higher in the ropeginterferon alfa-2b arm compared with the control treatment group (0.94 versus 0.82; log-rank test; p = 0.04) as shown by analysis of the time to first risk event.The Cox proportional hazard ratio was 0.34 (95% CI: 0.12-0.97;p = 0.04).Risk events occurred in 5/95 patients (5.3%) in the ropeginterferon alfa-2b arm (first events: thromboembolic events ) compared with 12/74 patients (16.2%) allocated to hydroxyurea/BAT (thrombotic events These results align with potentially improved survival in interferon-treated PV patients compared with hydroxyurea treatment or phlebotomy in prior observational studies [4,8].Supporting a treatment effect in the current prospective study, risk factors associated with a poor prognosis in PV were balanced between the treatment arms at study start, largely due to stratification during randomization.Furthermore, no selection bias due to the roll-over was detected in analyses of patient characteristics and response rates comparing patients who enrolled or did not enroll into CONTINUATION-PV [5].The final results of PROUD-PV/CONTINUATION-PV therefore indicate that the disease-modifying potential of ropeginterferon alfa-2b suggested by prior analyses [6] can now be quantified using hard clinical endpoints.
JAK2V617F allele burden is utilized as a surrogate endpoint for disease modification due to its central role in the pathophysiology of PV, and recent findings substantiate the notion that molecular response to treatment can alter the natural course of the disease [9].
While normalization of blood counts principally serves to minimize thrombotic risk in PV, leukocytosis has also been identified as a biomarker for disease evolution and overall survival [2,12].Longitudinal analysis of leukocytosis is essential, since a persistently elevated leukocyte trajectory is significantly associated with increased risk of disease progression (p = 0.0002) [12].Patients receiving ropeginterferon alfa-2b in the current studies not only achieved a greater reduction in leukocyte count compared to baseline versus control (mean absolute changes: −6.67 × 10 9 /L versus −3.59 × 10 9 /L respectively [p < 0.0001] at Month 72), but spent significantly more time with normal leukocyte counts compared with hydroxyurea/BAT treated patients (mean proportion of time in response: 93.7% vs 80.9%; p = 0.02).
Final safety data from up to 7.3 years' treatment in PROUD-PV and CONTINUATION-PV confirm the established positive safety profile of ropeginterferon alfa-2b [5,6].Only 14/127 (11.0%) of patients in the ropeginterferon alfa-2b arm and 3/127 (2.4%) in the hydroxyurea/ BAT arm discontinued due to drug-related toxicity; the higher discontinuation rate in the ropeginterferon alfa-2b arm may reflect the intensive monitoring of certain interferon-alfa class effects, which mandated drug withdrawal according to the protocol.AEs were predominantly of mild or moderate severity, with comparable rates of grade ≥3 treatment-related AEs between the groups over the entire period (ropeginterferon alfa-2b: 15.7%; control: 16.5%).Furthermore, PV-associated AEs declined during ropeginterferon alfa-2b treatment, occurring in 7.1% of patients in the 6th year compared with 12.1% in the control arm.
Normal life expectancy may be achievable in PV based on comparable overall survival in interferon-treated patients and a matched US population (p = 0.3), contrasting with shortened survival (p = 0.03) in non-interferon treated patients at the same academic center [3].Although overall survival was not evaluated, our findings lend support to this view, providing the first evidence that the durable hematologic and molecular responses observed with long-term ropeginterferon alfa-2b therapy are accompanied by improved event-free survival.This potential advantage should be considered when evaluating the individual risk-benefit relationship for ropeginterferon alfa-2b treatment in patients with PV.

Fig. 2
Fig. 2 Time spent in complete hematologic response by treatment arm.Time spent in complete hematologic response based on ELNdefined targets for peripheral blood cell counts (hematocrit <45% without phlebotomy for ≥3 months, platelet count <400 × 109/L and leukocyte count <10 × 109/L) for individual patients with PV in the CONTINUATION-PV full analysis set (ropeginterferon alfa-2b arm: panel A; control arm: panel B).Patients are ordered by proportion of time in response (highest to lowest).Blue bars indicate a complete hematologic response at the latest available assessment; yellow bars indicate no response at the latest assessment.