Abstract
Dynamic interactions between organelles are responsible for a variety of intercellular functions, and the endoplasmic reticulum (ER)–mitochondrial axis is recognized as a representative interorganelle system. Several studies have confirmed that most proteins in the physically tethered sites between the ER and mitochondria, called mitochondria-associated ER membranes (MAMs), are vital for intracellular physiology. MAM proteins are involved in the regulation of calcium homeostasis, lipid metabolism, and mitochondrial dynamics and are associated with processes related to intracellular stress conditions, such as oxidative stress and unfolded protein responses. Accumulating evidence has shown that, owing to their extensive involvement in cellular homeostasis, alterations in the ER–mitochondrial axis are one of the etiological factors of tumors. An in-depth understanding of MAM proteins and their impact on cell physiology, particularly in cancers, may help elucidate their potential as diagnostic and therapeutic targets for cancers. For example, the modulation of MAM proteins is utilized not only to target diverse intracellular signaling pathways within cancer cells but also to increase the sensitivity of cancer cells to anticancer reagents and regulate immune cell activities. Therefore, the current review summarizes and discusses recent advances in research on the functional roles of MAM proteins and their characteristics in cancers from a diagnostic perspective. Additionally, this review provides insights into diverse therapeutic strategies that target MAM proteins in various cancer types.
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Introduction
An understanding of the cooperation between organelles is crucial for revealing the mechanisms that modulate cellular functions and homeostasis. Among interorganellar networks, the connection between the endoplasmic reticulum (ER) and mitochondria has been extensively studied owing to its diverse functions and impact on the pathogenesis of multiple diseases. The concept of a functional unit comprising the ER and mitochondria was first proposed in 19501. The adjacent membrane sites that physically tether the ER and mitochondria are called mitochondria-associated ER membranes (MAMs); technological advances in microscopy have enabled the elucidation of the physiological features of the tethering structures of the MAMs. The ER and mitochondria are separated by a 6–15 nm gap, and the average surface area percentage of mitochondria covered by MAMs was calculated to be 3–5% in mammalian cells2.
MAMs represent an etiological and therapeutic target in cardiovascular diseases3, neurodegenerative diseases4, metabolic disorders5,6, and cancers. In this review, we discuss the associations between alterations in MAM proteins and cancers and present recent advances in research on these associations. Additionally, we discuss the contribution of MAM proteins to tumorigenesis and cancer progression as well as their possible applications as diagnostic and therapeutic targets.
Structure and functional role of ER–mitochondria contact sites
Calcium regulation
Maintenance of Ca2+ homeostasis is one of the most important functions of MAMs, as the ER functions as the main regulator and storage organelle of calcium ions within living cells7. The resting levels of Ca2+ in mitochondria are similar to those in the cytosol; however, they can increase to 100 times the cytosolic levels under specific stimulation conditions8. A contributing factor to this drastic increase has been identified and subsequently confirmed by the Ca2+ microdomain hypothesis, which states that the outer membrane of mitochondria contains hotspots for Ca2+ shuttling from the ER9,10,11. As the affinity of the mitochondrial calcium uniporter (MCU) located in the inner mitochondrial membrane is dependent on the local Ca2+ concentration, these microdomains facilitate Ca2+ influx through the MCU12,13.
The translocation of Ca2+ in MAMs is mediated by several proteins. The inositol 1,4,5-trisphosphate (IP3) receptor (IP3R) is a representative Ca2+ channel located in the ER14 (Fig. 1). The opening of this receptor and subsequent Ca2+ transport occur when the binding site of each tetrameric subunit of IP3R is concatenated with IP315. The IP3 binding affinity and Ca2+ influx activity of IP3R vary depending on its subtype16, phosphorylation17, and interactions with other regulatory proteins. Additionally, recent research has shown that the localization of mobile IP3R on MAMs is important for Ca2+ signaling between the ER and mitochondria18.
The canonical microdomain of the Ca2+ regulator in MAMs consists of IP3R located in the ER, voltage-dependent anion channel 1 (VDAC1) in the outer mitochondrial membrane (OMM), and glucose-regulated protein 75 (GRP75), which acts as a physical link between IP3R and VDAC1 and directly affects mitochondrial Ca2+ accumulation19. The formation of these complexes brings the ER and mitochondria into close proximity, resulting in the formation of microdomains with high Ca2+ levels20,21. Recent evidence has indicated the importance of another protein component within this microdomain. DJ-1 was recognized as the fourth component of the MAM complex through the observation that DJ-1 ablation induced IP3R3 aggregation, which prevented the tethering of the IP3R-GRP75-VDAC microdomain22.
In addition to the IP3R-GRP75-VDAC1 complex, the interaction of ER-integrated protein vesicle-associated membrane protein B (VAPB) with the OMM protein called protein tyrosine phosphatase-interacting protein-51 (PTPIP51) is also involved in Ca2+ regulation. Depletion of either VAPB or PTPIP51 leads to the disruption of MAMs and perturbation of Ca2+ transport23.
Lipid metabolism
Because lipid synthesis is compartmentalized, lipids must be transferred between organelle compartments. Lipids shuttle between specific organelles through vesicle trafficking; however, lipid influx into mitochondria through vesicles is not possible even when lipids are needed24. Thus, several MAM proteins regulate the nonvesicular trafficking of lipids from the ER to mitochondria (Fig. 1). Phosphatidylserine synthase 1/2 (PSS1/2) is a representative synthetic enzyme that is enriched in MAMs and mediates phosphatidylserine (PS) synthesis25. Specifically, PSS1 and PSS2 convert phosphatidylcholine (PC) and phosphatidylethanolamine (PE), respectively, into PS. PE import relies on the conversion of transported PS in MAMs to PE by PS decarboxylase (PSD) in mitochondria rather than direct import26. Disruption of this process and the mitochondrial PE level impairs mitochondrial dynamics and bioenergetics27,28. Mitochondrial PE can be traced back to MAMs and is converted into PC by PE-N-methyltransferase29. This transfer system is the rate-limiting step in lipid biogenesis and further contributes to the maintenance of phospholipid homeostasis.
The complex consisting of Mdm10 and Mdm34 is located in the OMM, and Mmm1 in the ER and Mdm12 in the cytosol exhibit features of ER–mitochondria tethering proteins and phospholipid exchangers30. Mdm34, Mmm1, and Mdm12 physically interact with phospholipids via their synaptotagmin-like mitochondrial lipid-binding domains31,32,33. The direct binding of Mmm1 and Mdm12 forms a hydrophobic cavity that mediates the transport of glycerophospholipids except for PE34. However, as the depletion of this complex only exerts minor effects on the lipidome, more unknown lipid regulatory proteins and mechanisms may exist in MAMs30.
Regulation of mitochondrial dynamics
Mitochondrial quality control is a defense mechanism against mitochondrial insult. In the early stages of quality control, translocation and recruitment of dynamin-related protein (DRP1) in mitochondria occurs in MAMs and facilitates mitochondrial fission35. In contrast, mitofusin 1 (MFN1), another MAM protein, forms puncta in the ER and facilitates mitochondrial fusion36. Physical tethering of the ER to mitochondria by MFN1/2 indicates the importance of MAMs as key sites for regulating mitochondrial dynamics37,38 (Fig. 1).
In addition to fission and fusion, self-degradation of mitochondria upon severe injury, a process called mitophagy, is also influenced by MAM proteins. The PTEN-induced putative kinase (PINK)/parkin pathway is the main signaling pathway for mitophagy. PINK is degraded by mitochondria-resident enzymes and further degraded in lysosomes under normal conditions; however, mitochondrial dysfunction leads to the formation of uncleaved PINK and its accumulation in the OMM through an interaction with TOM39. The accumulated PINK proteins recruit parkin, which induces mitophagy through its E3 ligase activity39,40. A recent study reported that PINK1/Parkin mediate MFN2 phosphorylation, resulting in the dissociation of the MFN2 complex via the p97-dependent pathway. This indicates a relationship between a decrease in ER–mitochondrial contact and mitophagy41. Additionally, assembly of the autophagosome marker ATG14 occurs in MAMs under starvation conditions, and disruption of the ER–mitochondria interaction inhibits ATG14 localization and autophagosome formation42.
Interaction between the ER–mitochondrial axis and calcium homeostasis
Most ER proteins are involved in regulating Ca2+ homeostasis43. For instance, the sigma-1 receptor (Sig-1R), a MAM protein, is enriched in the ER vesicles involved in this process. In the resting state, Sig-1R binds to another chaperone in the ER, GRP78. However, this complex dissociates under ER stress conditions, including Ca2+ depletion. The dissociated Sig-1R then binds to IP3R, mediating its stabilization and Ca2+ influx44. PDZ domain-containing protein 8 (PDZD8) in the ER is another example of a Ca2+-regulating protein in MAMs. PDZD8 knockdown impairs ER–mitochondria tethering and further inhibits mitochondrial Ca2+ uptake in the MAMs of mammalian neurons45. Other proteins modulate calcium homeostasis in MAMs, as shown in Table 1.
As Ca2+ participates in diverse cellular processes, disrupted homeostasis and improper regulation of Ca2+ dynamics in MAMs can negatively affect cellular function. The influx of Ca2+ into mitochondria is essential for bioenergetics because several intramitochondrial enzymes associated with glycolysis and the tricarboxylic acid cycle are activated in a calcium-dependent manner46. The lack of constitutive Ca2+ influx through IP3 reduces the enzymatic activity of pyruvate dehydrogenase and thus the production of adenosine triphosphate (ATP), resulting in the activation of autophagy via the AMPK pathway47. Translocase of mitochondrial outer membrane 70 (TOM70) also affects constitutive Ca2+ shuttling by mediating the linkage between IP3R3 and VDAC, and the depletion of TOM70 results in impaired mitochondrial respiration48.
MAM proteins stimulate Ca2+-dependent apoptotic pathways. Ca2+ overload in the mitochondrial matrix increases mitochondrial permeability by opening mitochondrial permeability transition pores (mPTPs)49. One mechanism of permeability transition is Ca2+-inducible conformational alteration of F-ATP synthases that bind to and show activity toward mPTPs50. The opening of mPTPs disrupts the osmotic balance in mitochondria due to nonselective permeabilization, resulting in an influx of water that induces the release of caspase cofactors51. Furthermore, alterations in Ca2+ levels are closely associated with responses to multiple intracellular stresses, such as ER and oxidative stress.
ER–mitochondria contacts modulate oxidative stress
Oxidative stress results from an imbalance between the production and accumulation of reactive oxygen species (ROS) in cells and is a hallmark of the ability to detoxify or repair reactive products52. ROS are produced primarily in mitochondria and play important roles in cell growth, differentiation, and death53,54. Although low levels of ROS play an essential role in intracellular signaling and pathogen defense, elevated levels can have detrimental effects on cells, such as decreasing the efficiency of mitochondrial respiration and inducing oncogenic stress55. Imbalances in ROS accumulation can contribute to the development and progression of several diseases, including cancer, metabolic disorders, diabetes, and cardiovascular diseases56,57.
The ER and mitochondrial axes play essential roles in the detection of and response to stress conditions, including oxidative stress, and form interconnected networks58. Furthermore, the simultaneous induction of ER stress and overproduction of ROS in several diseases highlights the importance of this axis59. The roles of ROS-related MAM proteins, including endoplasmic reticulum oxidoreductase 1 (ERO1), Sig-1R, p66Shc, and MFN2, have been reported (Fig. 2).
ERO1 is located entirely on the MAMs close to the ER surface and is an essential factor in the ER oxidative folding mechanism through co-localization with protein disulfide isomerase (PDI)60. PDI catalyzes the formation of disulfide bonds in unfolded proteins during oxidative protein folding and is then converted to a reduced form61. Reduced PDI is subsequently oxidized by ERO1 to participate in the catalytic reaction cycle, where reduced ERO1 transfers electrons to an oxygen molecule via flavin adenine dinucleotide, releasing H2O260. ERO1α, an ERO1 isoform, is overexpressed in various cancers, and its expression is increased by chronic ER stress, resulting in excessive H2O2 production and an increased ROS burden62. ERO1 also affects ROS production by regulating other MAM proteins. Under stress conditions, ERO1 oxidizes IP3R1 and induces detachment of the disulfide isomerase–like protein ERp44 from IP3R163. ERp44 has an inhibitory effect on IP3R164, leading to massive influx of Ca2+ through IP3R, which ultimately results in upregulated mitochondrial metabolism and excessive ROS production65,66.
Sig-1R regulates Ca2+ homeostasis and is involved in ROS-related signaling pathways. Although the ROS-regulatory mechanisms of Sig-1R are not fully understood, previous studies have shown that Sig-1R knockdown leads to ROS accumulation67,68. Furthermore, some Sig-1R agonists exhibit antioxidant activity under pathological conditions69.
p66Shc is located in MAMs, mitochondria, and the cytosol and tetramerizes in response to oxidative stress70. Under oxidative stress conditions, its Ser36 residue is phosphorylated by p38MAPK, ERK, and JNK1/2, and phosphorylation of other residues, namely, Ser54 and Thr386, occurs to prevent p66Shc degradation by ubiquitination71,72,73. Activated p66Shc translocates through MAMs into mitochondria, where it binds to cytochrome c to generate ROS and ultimately induce cell death74. The generation of ROS by activated p66Shc is supported by previous studies showing that both p66Shc knockout mice and cells exhibit reduced oxidative stress levels and a decreased incidence of diseases such as atherosclerosis75,76.
As previously described, both MFN1 and MFN2 are involved in the promotion of mitochondrial fusion. However, the fusion process, which relies primarily on MFN1 and MFN2, is speculated to have additional distinct functions77. The possible effects of MFN2 on ROS generation have been suggested to be due to other functions of MFN2. Munoz et al.78 reported the possible inhibitory effects of MFN2 on ROS production. MFN2 directly interacts with an ER stress branch, the pancreatic endoplasmic reticulum kinase (PERK) pathway, and inhibits ER stress pathways and ROS production. Other studies have shown that MFN2 overexpression activates the PERK/activating transcription factor 4 (ATF4) pathway and reduces ROS levels in cardiac fibroblasts79. However, a recent study showed that MFN2 facilitates the adaptation of macrophages to mitochondrial respiration and ROS generation in response to inflammatory stimuli80. Thus, further research is required to fully understand the different functions of MFN2 in different cell types and under specific stress conditions.
Interaction between ER stress and the ER–mitochondria axis
Protein folding is the main function of the ER. Various conditions, such as disruption of Ca2+ homeostasis, inhibition of degradation of unfolded proteins due to proteasome blockade, and genetic mutations, can cause the accumulation of unfolded proteins81. Under these stress conditions, the unfolded protein response (UPR) is activated by three ER transmembrane proteins: activating transcription factor 6 (ATF6), inositol-requiring enzyme 1α (IRE1α), and PERK82. Under normal conditions, the ER chaperone GRP78/BiP binds to the ER lumen region of these transmembrane proteins and inhibits their activity. However, under stress conditions, GRP78 binds to misfolded proteins and induces the activation of these three transmembrane proteins83.
In the ATF6 pathway of the ER stress response, sensors mediate the UPR, and ATF6 translocates to the Golgi complex after GRP78 is released. ATF6 is first cleaved by site-1 protease, and one half remains at the NH2-terminus before being cleaved by site-2 protease84. Regarding the IRE pathway, GRP78 is normally bound to IRE1α or its homolog, IRE1p, and maintains its inactivation. When GRP78 dissociates from IRE1 in ER-stressed cells, IRE1 is phosphorylated and dimerizes85. Finally, activated PERK phosphorylates eIF2α and further increases the translation of selected mRNAs, including ATF4, which then promotes the expression of transcription factors, such as C/EBP homologous protein (CHOP), leading to growth arrest and DNA damage86. CHOP overexpression causes apoptosis by translocating B-cell lymphoma 2 (BCL2)-associated X (a proapoptotic protein) to mitochondria and decreasing the expression of BCL2 (an antiapoptotic protein)87.
The associations between MAM components and ER stress have been widely reported (Table 2), and some UPR-related proteins also function as MAM components. The interaction between PERK and MFN2 is a representative example of the UPR-related MAM pathway. Additionally, some MAM proteins are regulated by ER stress; for instance, Sig-1R is transcriptionally upregulated via the PERK/eIF2α/ATF4 pathway88, while another MAM protein, Rab32, is upregulated via the UPR pathway. Rab32 belongs to the Ras-like small GTPase family and is involved in mitochondrial fission via interaction with DRP189. In SH-SY5Y cells, Rab32 expression is elevated upon induction of ER stress (thapsigargin treatment), leading to mitochondrial dysfunction and neuronal death90. Furthermore, the ER chaperone GRP78 binds to IP3R1 during the ER stress response, releasing Ca2+ for influx into mitochondria and inducing cell death due to mitochondrial dysfunction91.
Further evidence has also revealed that several MAM proteins affect UPR pathways. The ER protein VAPB is an important protein involved in UPR activity, and VABP loss inhibits IRE1/XBP1 activity in response to ER stress92. Furthermore, VAPB interacts with ATF6 in response to ER stress, and the terminal domain of ATF6 senses protein accumulation in the ER lumen. VAPB, with no luminal structure, is not directly regulated by ATF6 activation but is indirectly inhibited93. VAPB-induced ER stress has been implicated in inducing mitochondrial dysfunction by releasing Ca2+ through interactions with PTPIP51 in the mitochondrial membrane23.
Characteristics and diagnostic role of ER–mitochondria contact sites in cancers
Cancer cells require a substantial amount of energy for their rapid proliferation and acquisition of malignant phenotypes and use various methods, such as increases in glucose uptake and glycolytic activity (a phenomenon known as the Warburg effect), lipid synthesis and lipolysis, and modulation of Ca2+ signaling, to meet these requirements94,95,96. Therefore, MAMs play important roles in cancer cell function and metabolism, as they regulate the aforementioned pathways (Fig. 3).
The regulation of Ca2+ signaling is crucial in cancers, as it is involved in cancer progression, epithelial-to-mesenchymal transition, invasion, and resistance to apoptosis97. Therefore, Ca2+-regulating proteins in MAMs play various roles in cancer development (Table 3). The IP3R-GRP75-VDAC-MCU complex, which plays an important role in Ca2+ transport, is regulated by oncoproteins such as PTEN, BRCA1, and BCL298. In MAMs, PTEN binds to IP3R and prevents its degradation, thereby promoting Ca2+ transport to mitochondria, which is important for apoptosis98,99. However, in various cancers, PTEN loses functionality and triggers inappropriate Ca2+ transport, leading to apoptosis resistance100,101. BCL2, another oncoprotein in MAMs, interacts with IP3R and VDAC and prevents the translocation of Ca2+ from the ER to mitochondria. Furthermore, the interaction between BCL2 and VDAC1 interferes with the export of cytochrome c from mitochondria and thus hinders apoptosis98,102. Therefore, BCL2 overexpression in cancers results in resistance to apoptosis.
BRCA1-associated protein 1 (BAP1), a tumor suppressor protein in MAMs, facilitates Ca2+ influx into mitochondria by interacting with IP3R103. Abnormalities in the function of BAP1 can induce inappropriate Ca2+ influx into mitochondria, which may affect the regulation of apoptosis and lead to carcinogenesis104. Mutations in BAP1 have been observed in various cancers, including renal cell carcinoma, cutaneous melanoma, and uveal melanoma104. GRP75 also plays an important role in the regulation of Ca2+ homeostasis2. Transglutaminase type 2 modulates GRP75 function by binding to GRP75 and increasing Ca2+ flux between the ER and mitochondria, which affects cancer growth and metastasis. Upregulation of transglutaminase type 2 is a hallmark of breast cancer105,106. Additionally, TOM70, a protein that links IP3R3 to VDAC, exhibits notably high expression levels in breast cancer cells, and its potential as a therapeutic target has been duly recognized in previous studies48,107.
In addition to its proapoptotic role in mitochondria, Ca2+ is important for energy production, progression, and metastasis in cancer108,109. Ca2+ influx into mitochondria mediated by MCU promotes mitochondrial biogenesis and colon cancer proliferation108, and impairment of Ca2+ uptake by MCU knockdown inhibits the proliferation of embryonal rhabdomyosarcoma110. Other types of cancers with high MCU expression include prostate, ovarian, and breast cancers, indicating the diagnostic utility of MCU expression in cancer111. Moreover, PDZD8, another Ca2+-regulating protein in MAMs, was found to exhibit increased expression levels in stomach cancer tissue compared with normal tissue and is involved in the proliferation and metastasis of stomach cancer112.
Although research on the exact role of ROS in cancers is still underway, ROS are known to be involved in cancer progression and metastasis98. Several MAM proteins, including p66hsc, are regulated by ROS, and p66hsc and the oncoprotein p53 regulate each other113. Furthermore, p66hsc can be activated by steroid hormones, and activated p66hsc interacts with cytochrome c to increase ROS production. These alterations, including oxidative stress, have been reported to result in poor prognosis in patients with prostate cancer72,114,115,116. These characteristics of p66hsc have also been observed in other cancers, including breast and lung cancers, indicating its potential as a diagnostic and therapeutic target117,118,119. Furthermore, ERO1, which controls ROS production through the regulation of MAM proteins, is overexpressed in cholangiocarcinoma and is involved in proliferation and metastasis, leading to poor prognosis in patients120. Notably, ERO1 is also overexpressed in various other cancers, including breast cancer, lung cancer, and hepatocellular carcinoma, in which it ultimately results in poor prognosis121,122,123.
Activated lipid metabolism and the accumulation of lipid droplets are hallmarks of various cancer cells95. Elevated lipid levels in cancer cells promote proliferation and serve as energy reserves and messengers in oncogenic pathways95,124. Furthermore, various enzymes involved in lipid synthesis are upregulated in various cancers, including lung, ovarian, and prostate cancers95,125,126. Various enzymes involved in lipid synthesis, such as fatty acid CoA ligase, which catalyzes the ligation of triacylglycerols and ceramide, and acyl-coenzyme A:cholesterol acyltransferase-1 (ACAT-1), which catalyzes the synthesis of cholesterol, are mainly located in MAMs98,127,128. Therefore, alterations in the expression of these enzymes in MAMs are strongly associated with cancer. For example, after passing through mitochondria, ceramide plays an important role as an apoptosis inducer and can inhibit cancer growth and cell death129,130. Cholesterol metabolism is strongly associated with cancer. ACAT-1 in MAMs converts cholesterol to cholesteryl esters, which accumulate in the lipid droplets of cancer cells98,131. These accumulated cholesteryl esters have a considerable impact on the proliferation and metastasis of cancer cells132. Caveolin-1, located in MAMs, is involved in cholesterol efflux, and its overexpression has been identified in a variety of cancers, such as lung, liver, kidney, and colon cancers133. These expression patterns of caveolin-1 are closely related to cancer progression, metastasis, and drug resistance134,135,136. Therefore, various MAM proteins play major roles in cancer and can potentially be used in diagnosis and treatment. The association between ER stress and cancer has been established137. Sig-1R, a MAM protein regulated by ER stress, has been reported to be overexpressed in myelogenous leukemia and colon cancer138. This increased expression promotes angiogenesis and facilitates cancer cell migration, resulting in poor prognosis in patients. Consequently, Sig-1R is considered a promising therapeutic target138. Another MAM protein associated with ER stress, VAP-B, has been reported to play a key role in breast cancer progression, highlighting its potential as a diagnostic marker for this malignancy139.
The ER–mitochondrial axis as a therapeutic target
Targeting Ca2+ signaling
The characteristic functions of MAMs, including those in Ca2+ and ROS signaling, lipid metabolism, autophagy, and mitochondrial fission, enable their use as diagnostic markers and therapeutic targets for cancer (Fig. 3). Different methods can be used to trigger cancer cell apoptosis by promoting Ca2+ transport through modulation of MAM proteins. One of the most widely used anticancer drugs, cisplatin, is used to treat various cancers, including ovarian, breast, lung, and bladder cancers140. In ovarian cancer (SKOV3) cells, cisplatin promotes Ca2+ translocation from the ER to mitochondria and cytosol, causing ER stress-mediated apoptosis141. Other cancer therapeutics, such as adriamycin and mipsagargin, target Ca2+ signaling. In MAMs, p53 regulates the activity of SERCA by binding to it, leading to Ca2+ influx into the ER and resulting in increased apoptosis142. p53 mutations have been detected in various types of cancers, and adriamycin can increase p53 levels in MAMs, which promotes Ca2+ signaling and apoptosis in cancer cells through the activation of SERCA111,142,143. Mipsagargin inhibits SERCA function, resulting in an increase in intracellular Ca2+, which induces apoptosis in cancer cells144. Another component of the Ca2+ transport complex, VDAC, can potentially serve as a biomarker and therapeutic target for breast cancer, as its overexpression was detected in a previous study145. Furthermore, VDAC1 inhibition by siRNA induces cancer cell apoptosis, suggesting that siRNAs could be a target for cancer therapy146,147. Previous studies have shown that PDZD8, which is highly expressed in stomach cancer and is involved in cancer cell proliferation and metastasis, can also be used as a therapeutic target112. Notably, sunitinib, a kinase inhibitor, attenuates the proliferation of stomach cancer cells, as demonstrated in the human gastric cancer cell lines TMK1 and MKN74, by decreasing the PDZD8 protein level112.
Targeting lipid metabolism and ER stress
Targeting the lipid metabolism-related functions of MAMs could aid in cancer treatment. For example, mitotane, which targets ACAT-1, converts cholesterol to CE and causes lipid droplet formation in various cancers148. In adrenocortical carcinoma, mitotane-induced ACAT-1 suppression induces free cholesterol and fatty acid accumulation in the ER, leading to apoptosis111,148,149. Modulation of ER stress also constitutes a potential therapeutic approach for cancer. In prostate cancer, corosolic acid modulates IRE1 and PERK signaling and induces ER stress, which promotes apoptosis and inhibits cell proliferation150. In hepatocarcinoma, 20(S)-protopanaxadiol can increase UPR activity and enhance the ER stress response by phosphorylating components of the PERK cascade, subsequently leading to increases in the expression of associated genes151. Moreover, previous studies have shown that panaxydol induces Ca2+ release from the ER through IP3R and activates the JNK pathway, causing ER stress, which is mediated by PERK152,153. These effects trigger apoptosis in renal carcinoma and prostate cancer cells152,153. Evodiamine is another therapeutic candidate that affects the JNK and PERK pathways. By modulating both pathways, evodiamine can induce apoptosis in ovarian cancer cells and reduce the extent of metastasis in colon cancer153,154,155.
Increasing the sensitivity of cancer cells to chemotherapeutic compounds
Repeated use of chemotherapeutic drugs can result in resistance to them; therefore, other MAM proteins can be targeted to reduce this resistance. For example, cisplatin is widely used to treat ovarian cancer; however, its long-term use can induce cisplatin resistance in ovarian cancer cells, which is highly correlated with GRP75156. GRP75 knockdown increases cisplatin-induced apoptosis in ovarian cancer cells, suggesting a decrease in resistance157,158. Blocking the function of MAM-localized BCL2, which interacts with IP3R, via a BCL2 inhibitor disrupts Ca2+ translocation and leads to an increase in the cellular Ca2+ level in cisplatin-resistant ovarian cancer cells. Furthermore, a study demonstrated that ABT737, a BCL2 inhibitor, reduces the cisplatin resistance of SKOV3 ovarian cancer cells by modulating Ca2+ signaling159,160. These changes in cellular Ca2+ signaling lead to cisplatin-induced apoptosis, indicating that the regulation of MAM proteins could lower resistance to anticancer agents159,160. Targeting MAM-localized PERK is also a potential approach for treating resistant forms of cancers. PERK regulates ER stress, ROS production, and Ca2+ levels, all of which affect the apoptotic process59,161. Previous studies have reported that modulating PERK can induce apoptosis in endocrine-resistant breast cancer cells161,162,163. Another example of increased anticancer treatment sensitivity is that occurring after combination treatment with bortezomib, a proteasome inhibitor, and cisplatin. In pancreatic cancer, bortezomib can maximize the anticancer effects of cisplatin by activating JNK cascades and subsequently inducing apoptosis159,164.
Reducing cancer metastasis
Emerging evidence suggests that MAM proteins and mitochondrial calcium dynamics may affect the migratory ability of cells165, and several studies have revealed the roles of MAM proteins in tumor invasion and metastasis, offering valuable perspectives for both diagnostic and therapeutic approaches. In triple-negative breast cancer cells, blocking MCU function can inhibit Ca2+ influx into mitochondria and ROS generation, resulting in reduced migration and progression159,166. Moreover, overexpression of FUN14 domain-containing 1 (FUNDC1), a MAM protein, could be a diagnostic and prognostic marker for breast cancer, as it triggers cell proliferation, migration, and invasion167. FUNDC1 knockdown by siRNA alters NFATC1 activity and inhibits the proliferation and metastasis of breast cancer cells147,168. A similar example is the targeting of MFN2 by miR-761 in hepatocellular carcinoma discovered in a previous study165, wherein miR-761 was shown to be upregulated in the tissues of patients with hepatocellular carcinoma, thereby confirming its role in modulating MFN2 expression. Additionally, miR-761 inhibition resulted in reduced migration and invasion of human cancer cell lines, as well as suppression of tumor metastasis in nude mice165.
Increasing immune cell activity
Modulation of MAMs may aid in cancer treatment by increasing the accessibility of immune cells. For example, interactions between the ER and mitochondria regulate the expression of glycans, which can reduce immune cell accessibility in glioblastoma169. A previous study proposed the modification of glycan expression in glioblastoma through modulation of ER–mitochondria contact sites to enhance immune cell recognition as a potential approach for glioblastoma treatment169. Furthermore, regulation of MAM proteins in immune and cancer cells can aid in treatment. In memory T cells, promoting AKT signaling can inhibit the expression of MAM-localized GSK3b and strengthen the interaction between VDAC and HK-1, resulting in increased cellular respiration and functional acquisition170. These alterations play a significant role in the differentiation of memory T cells into effector T cells170. These studies suggest that the modulation of MAM proteins to increase immune cell activity offers various therapeutic benefits.
Conclusion
Interactions between organelles are involved in many cellular functions. This review focuses specifically on the contact sites between the ER and mitochondria, known as MAMs. Various MAM proteins play important roles in the regulation of Ca2+ signaling, lipid metabolism, mitochondrial dynamics, oxidative stress, and ER stress. Therefore, alterations in MAM proteins can lead to changes in these mechanisms, resulting in the inhibition of apoptosis and increased resistance to anticancer drugs. Several therapeutic agents targeting MAM proteins have been reported to induce apoptosis and reduce antibiotic resistance and metastasis in cancer cells by modulating Ca2+ signaling and lipid metabolism. Owing to these diverse effects in cancers, research on MAM-targeting therapeutics should be ongoing. Moreover, as alterations in MAM proteins are characteristic of various cancers, they can potentially serve as diagnostic markers and therapeutic targets; however, further research is needed to determine whether they can be used as accurate biomarkers for specific cancers.
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Acknowledgements
This work was supported by National Research Foundation of Korea (NRF) grants funded by the Korean government (MSIT) (grant numbers: 2021R1A2C2005841 and 2021R1C1C1009807). Additionally, this research was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI22C1424).
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G.A., J.P. J.S., and T.H. wrote the manuscript and prepared the figures and tables. G.A. and J.P. edited the manuscript and figures. G.S. and W.L. conceived, structured and edited the manuscript.
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An, G., Park, J., Song, J. et al. Relevance of the endoplasmic reticulum-mitochondria axis in cancer diagnosis and therapy. Exp Mol Med 56, 40–50 (2024). https://doi.org/10.1038/s12276-023-01137-3
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DOI: https://doi.org/10.1038/s12276-023-01137-3