Abstract
The ultrasonic scission was studied for deoxyribonucleic acid (DNA) and five polyribonucleotide duplexes; three antiparallel-stranded helices (Poly(G)·Poly(C), Poly(A)·Poly(U), Poly(I)·Poly(C)) and two parallel-stranded helices (Poly(C)·Poly(C+) and Poly(A)·Poly(A+)). The sonication was carried out in 0.1—0.2 M NaCl solutions at 0°C and 200 watts for totally 20 minutes under helium gas atmosphere. The sonicated sample was fractionated by successive precipitation with acetone as the precipitant. For each duplex, several fractions were obtained in large quantities. The weight-average molecular weight Mw, the degree of polydispersity Mw/Mn, and the molecular weight distribution were determined for unsonicated, sonicated, and fractionated samples by the gel-permeation-chromatography/low-angle-laser-light-scattering method. The Mw values were reduced to a limit of 105 and the Mw/Mn values were narrowed to a 2—1.5 range by sonication. These values were further refined by fractionation. The molecular weight distribution profiles of well-defined fractions could be fitted by the logarithmic-normal function. The relationship between intrinsic viscosity and Mw was evaluated for each duplex. The result showed that the rigidity or flexibility of polymer chain depends on the molecular weight of fractionated samples and the kind of duplex examined.
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Tanigawa, M., Mukaiyama, N., Shimokubo, S. et al. Molecular Weight Distribution and Intrinsic Viscosity of Sonicated and Successively Fractionated Double-Stranded Deoxyribonucleic Acid and Polyribonucleotides. Polym J 26, 291–302 (1994). https://doi.org/10.1295/polymj.26.291
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DOI: https://doi.org/10.1295/polymj.26.291