Cell culture infection models help to develop antiviral agents, but animal models are required to understand complex virus–host interactions and the development of immune therapies. Although identification of the HBV uptake receptor enabled establishing cell lines that replicate HBV from its natural transcription template, animal models supporting the full HBV life cycle are still lacking.
This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
-
Clinical stage drugs targeting inhibitor of apoptosis proteins purge episomal Hepatitis B viral genome in preclinical models
Cell Death & Disease Open Access 23 June 2021
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$29.99 / 30 days
cancel any time
Subscribe to this journal
Receive 12 print issues and online access
$209.00 per year
only $17.42 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Lempp, F. A. et al. Sodium taurocholate cotransporting polypeptide is the limiting host factor of hepatitis B virus infection in macaque and pig hepatocytes. Hepatology http://dx.doi.org/10.1002/hep.29112 (2017).
Yan, Z. et al. HBVcircle: a novel tool to investigate hepatitis B virus covalently closed circular DNA. J. Hepatol. http://dx.doi.org/10.1016/j.jhep.2017.02.004 (2017).
Yan, H. et al. Viral entry of hepatitis B and D viruses and bile salts transportation share common molecular determinants on sodium taurocholate cotransporting polypeptide. J. Virol. 88, 3273–3284 (2014).
Allweiss, L. & Dandri, M. Experimental in vitro and in vivo models for the study of human hepatitis B virus infection. J. Hepatol. 64, S17–S31 (2016).
Dandri, M. & Petersen, J. Chimeric mouse model of hepatitis B virus infection. J. Hepatol. 56, 493–495 (2012).
Guidotti, L. G., Matzke, B., Schaller, H. & Chisari, F. V. High-level hepatitis B virus replication in transgenic mice. J. Virol. 69, 6158–6169 (1995).
Sprinzl, M., Dumortier, J. & Protzer, U. Construction of recombinant adenoviruses that produce infectious hepatitis B virus. Methods Mol. Med. 96, 209–218 (2004).
Dion, S., Bourgine, M., Godon, O., Levillayer, F. & Michel, M. L. Adeno-associated virus-mediated gene transfer leads to persistent hepatitis B virus replication in mice expressing HLA-A2 and HLA-DR1 molecules. J. Virol. 87, 5554–5563 (2013).
Yang, P. L. et al. Immune effectors required for hepatitis B virus clearance. Proc. Natl Acad. Sci. USA 107, 798–802 (2010).
Huang, L. R. et al. Transfer of HBV genomes using low doses of adenovirus vectors leads to persistent infection in immune competent mice. Gastroenterology 142, 1447–1450.e3 (2012).
Acknowledgements
U.P. receives funding from the Institute for Advanced Study at Technische Universität München, Germany, supported via the German Excellence Initiative and EU 7th Framework Programme under grant agreement number 291763. Research in the Protzer laboratory is funded by the German Research Foundation through the collaborative research centres TRR36 and TRR179 and by individual grant PR 618/7, by EU H2020 grant Hep-CAR, by the German Center for Infection Research and by the Helmholtz Validation Fund.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The author declares no competing financial interests.
Related links
FURTHER INFORMATION
PowerPoint slides
Rights and permissions
About this article
Cite this article
Protzer, U. The bumpy road to animal models for HBV infection. Nat Rev Gastroenterol Hepatol 14, 327–328 (2017). https://doi.org/10.1038/nrgastro.2017.44
Published:
Issue Date:
DOI: https://doi.org/10.1038/nrgastro.2017.44
