Using autofluorescence imaging (AFI) either alone or in tandem with magnification narrow-band imaging had limited clinical accuracy in detecting high-grade dysplasia and early oseophageal adenocarcinoma in a prospective study of 42 patients with Barrett oesephagus. The two techniques had moderate interobserver agreement. Therefore, AFI does not seem to be useful for the detection of neoplastic lesions in patients with Barrett oesophagus.