Concentrations of liver enzymes in plasma are used as indicators of liver disease. A genome-wide association study of 61,089 individuals identified 42 loci (of which 32 were new) associated with plasma liver enzyme concentrations. Functional genomic approaches identified 69 candidate genes involved in mulitple cellular processes including biliary transport, inflammation, immunity and lipid, glucose and carbohydrate metabolism as well as several genes of unknown function.