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Elucidating the inosinome: global approaches to adenosine-to-inosine RNA editing

Nature Reviews Genetics volume 12, pages 81–85 (2011)Cite this article

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Abstract

Catalysed by members of the adenosine deaminase acting on RNA (ADAR) family of enzymes, adenosine-to-inosine (A-to-I) editing converts adenosines in RNA molecules to inosines, which are functionally equivalent to guanosines. Recently, global approaches to studying this widely conserved phenomenon have emerged. The use of bioinformatics, high-throughput sequencing and other approaches has increased the number of known editing sites by several orders of magnitude, and we now have a greater understanding of the control and the biological significance of editing. This Progress article reviews some of these recent global studies and their results.

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Figure 1: Adenosine-to-inosine editing and bioinformatics.
Figure 2: Padlock capture, amplification and sequencing of editing-site candidates.
Figure 3: Verification of editing sites by the inosine chemical erasing (ICE) method.

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Acknowledgements

B.-E.W. is a recipient of a University of Pennsylvania Vagelos scholarship. M.S. is supported in part by a fellowship from the Japan Society for the Promotion of Science. K.N. is supported by grants from the US National Institutes of Health, the Ellison Medical Foundation and the Commonwealth Universal Research Enhancement Program, Pennsylvania Department of Health.

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Authors and Affiliations

  1. Bjorn-Erik Wulff, Masayuki Sakurai and Kazuko Nishikura are at The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA.,

    Bjorn-Erik Wulff, Masayuki Sakurai & Kazuko Nishikura

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  1. Bjorn-Erik Wulff
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  2. Masayuki Sakurai
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  3. Kazuko Nishikura
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Correspondence to Bjorn-Erik Wulff or Kazuko Nishikura.

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Database of RNA Editing (DARNED)

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Wulff, BE., Sakurai, M. & Nishikura, K. Elucidating the inosinome: global approaches to adenosine-to-inosine RNA editing. Nat Rev Genet 12, 81–85 (2011). https://doi.org/10.1038/nrg2915

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