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Simultaneous measurement of sleep and feeding in individual Drosophila

Nature Protocols volume 12, pages 23552366 (2017) | Download Citation

Abstract

Drosophila is widely used for the dissection of genetic and neuronal mechanisms of behavior. Recently, flies have emerged as a model for investigating the regulation of feeding and sleep. Although typically studied in isolation, increasing evidence points to a fundamental connection between these behaviors. Thus, a system for measuring sleep and feeding simultaneously in a single integrated system is important for interpreting behavioral shifts of either state. Here, we describe the construction and use of the Activity Recording Capillary Feeder or CAFE (ARC), a machine-vision (automated image tracking)-based system for the integrated measurement of sleep and feeding in individual Drosophila. Flies feed on liquid food from a microcapillary, and consumption is measured by tracking the liquid meniscus over time. Sleep measurements are obtained from positional tracking of individual animals, and arousal threshold can be determined by vibrational stimulus response. Using this system, a single computer and experimenter can track diverse behaviors from up to 60 individual flies in a single integrated system. The ARC is efficiently assembled with minimal training, and each experiment can be run for up to 7 d, with a total setup and breakdown time of 2 h.

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Acknowledgements

We thank J. Jacobs and A. Sehgal for comments on the manuscript. This work was funded by the National Institutes of Health (R21DK092735 to W.W.J.).

Author information

Affiliations

  1. Department of Neuroscience, The Scripps Research Institute, Jupiter, Florida, USA.

    • Keith R Murphy
    • , Jin Hong Park
    •  & William W Ja
  2. Center on Aging, The Scripps Research Institute, Jupiter, Florida, USA.

    • Keith R Murphy
    • , Jin Hong Park
    •  & William W Ja
  3. Program in Integrative Biology and Neuroscience, Florida Atlantic University, Jupiter, Florida, USA.

    • Keith R Murphy
  4. Radcliffe Institute for Advanced Study, Harvard University, Cambridge, Massachusetts, USA.

    • Robert Huber
  5. J.P. Scott Center for Neuroscience, Mind and Behavior, Bowling Green State University, Bowling Green, Ohio, USA.

    • Robert Huber

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Contributions

K.R.M., J.H.P., R.H., and W.W.J. contributed to the conception, development, and testing of the ARC; K.R.M., R.H., and W.W.J. wrote the manuscript; K.R.M., J.H.P., R.H., and W.W.J. revised the manuscript.

Competing interests

The authors declare no competing financial interests.

Corresponding authors

Correspondence to Robert Huber or William W Ja.

Integrated supplementary information

Supplementary information

PDF files

  1. 1.

    Supplementary Text and Figures

    Supplementary Figures 1 and 2.

Zip files

  1. 1.

    Supplementary Data

    Compressed archive of ARC software. Archive contains seven .stl files for 3D printing of ARC components, jArduino firmware, the JavaGrinders framework and the ARC modules (ARCController and ARCControllerMultiCam), the Noah analysis program (Python-based), and a sample ARC data set of 30 Canton-S males over 2 d.

Videos

  1. 1.

    Video guide to using the ARC.

    Overview of the procedure.

About this article

Publication history

Published

DOI

https://doi.org/10.1038/nprot.2017.096

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