Abstract
The goal of many current studies of neovascularization is to define the phenotype of vascular cell populations of different origins and to determine how such cells promote assembly of vascular channel. Here, we describe a protocol to immunophenotype vascular cells by high-resolution imaging and by fluorescence-activated flow cytometry in an in vivo rodent model of pulmonary microvascular remodeling. Analysis of cells by this combined approach will characterize their phenotype, quantify their number and identify their role in the assembly of vascular channels.
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Acknowledgements
Supported by NIH R01HL 070866 (to RJ), NIH R01CA 115767 (to RKJ) and by a Career Development Award to DGD (from the American Association for Cancer Research-Genentech BioOncology).
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Jones, R., Capen, D., Cohen, K. et al. A protocol for phenotypic detection and characterization of vascular cells of different origins in a lung neovascularization model in rodents. Nat Protoc 3, 388–397 (2008). https://doi.org/10.1038/nprot.2007.537
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DOI: https://doi.org/10.1038/nprot.2007.537
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A protocol for a lung neovascularization model in rodents
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