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Preparative scale Baeyer–Villiger biooxidation at high concentration using recombinant Escherichia coli and in situ substrate feeding and product removal process

Abstract

An efficient biocatalytic process based on the use of adsorbent resin (in situ substrate feeding and product removal) makes experiments at high substrate concentration possible by overcoming limitations due to substrate and product inhibition. This process was successfully applied to the preparative scale Baeyer–Villiger biooxidation of (−)-(1S,5R)-bicyclo[3.2.0]hept-2-en-6-one (25 g). Whole cells of recombinant E. coli (1 liter) overexpressing cyclohexanone monooxygenase were used as a biocatalyst and the substrate was preloaded onto the adsorbent resin. The corresponding lactone was obtained in 75–80% yield. Time for cell growth and biotransformation is about 24 h each and oxygen supply can be improved by using a tailor-made bubble column.

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Figure 8: Kinetic profile of the BV oxidation of 25 g of (−)-(1S,5R)-1 in the 1-liter bubble column over two cycles: exchange of biocatalyst after 10 h.
Figure 9: GC analysis (Chirasil-Dex column, 110 °C, 1.2 bar He) of (−)-(1R,5S)-2 after bulb-to-bulb distillation.

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Acknowledgements

This work was supported by European Community Fifth Framework Program (QLK3-CT2001-00403). The Swiss Federal Office for Education and Science (BBW) and the Foundation Ramón Areces are acknowledged for postdoctoral fellowships.

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Correspondence to Véronique Alphand.

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One of the authors (RW) is employed by Sigma Aldrich Ldt. Another one (IH) was employed at that time. We collaborated via a European “demonstration” program (EC Framework 5). This company also provided us with most of chemicals and resins.

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Hilker, I., Gutiérrez, M., Furstoss, R. et al. Preparative scale Baeyer–Villiger biooxidation at high concentration using recombinant Escherichia coli and in situ substrate feeding and product removal process. Nat Protoc 3, 546–554 (2008). https://doi.org/10.1038/nprot.2007.532

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