Real-time mapping and manipulation of electrophysiology in three-dimensional (3D) tissues could have important impacts on fundamental scientific and clinical studies, yet realization is hampered by a lack of effective methods. Here we introduce tissue-scaffold-mimicking 3D nanoelectronic arrays consisting of 64 addressable devices with subcellular dimensions and a submillisecond temporal resolution. Real-time extracellular action potential (AP) recordings reveal quantitative maps of AP propagation in 3D cardiac tissues, enable in situ tracing of the evolving topology of 3D conducting pathways in developing cardiac tissues and probe the dynamics of AP conduction characteristics in a transient arrhythmia disease model and subsequent tissue self-adaptation. We further demonstrate simultaneous multisite stimulation and mapping to actively manipulate the frequency and direction of AP propagation. These results establish new methodologies for 3D spatiotemporal tissue recording and control, and demonstrate the potential to impact regenerative medicine, pharmacology and electronic therapeutics.
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The authors thank R. Liao and D. Zhang for the inspiring discussion on cardiac electrophysiology and tissue engineering. The authors thank J. L. Huang for the assistance on instrumentation. This work was supported by National Institutes of Health Director's Pioneer and National Security Science and Engineering Faculty Fellow awards (to C.M.L.).
The authors declare no competing financial interests.
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Dai, X., Zhou, W., Gao, T. et al. Three-dimensional mapping and regulation of action potential propagation in nanoelectronics-innervated tissues. Nature Nanotech 11, 776–782 (2016). https://doi.org/10.1038/nnano.2016.96
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