Abstract
Although neuronal stress circuits have been identified, little is known about the mechanisms that underlie the stress-induced neuronal plasticity leading to fear and anxiety. Here we found that the serine protease tissue-plasminogen activator (tPA) was upregulated in the central and medial amygdala by acute restraint stress, where it promoted stress-related neuronal remodeling and was subsequently inhibited by plasminogen activator inhibitor-1 (PAI-1). These events preceded stress-induced increases in anxiety-like behavior of mice. Mice in which the tPA gene has been disrupted did not show anxiety after up to three weeks of daily restraint and showed attenuated neuronal remodeling as well as a maladaptive hormonal response. These studies support the idea that tPA is critical for the development of anxiety-like behavior after stress.
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Acknowledgements
This study was supported by National Institutes of Health grants NS-35704 and NS-38472. We thank Z-L. Chen for sharing his expertise in immunohisto-chemistry, P. Mercado and Y. Keptsi for technical assistance, and the members of Strickland Lab for discussion.
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Pawlak, R., Magarinos, A., Melchor, J. et al. Tissue plasminogen activator in the amygdala is critical for stress-induced anxiety-like behavior. Nat Neurosci 6, 168–174 (2003). https://doi.org/10.1038/nn998
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DOI: https://doi.org/10.1038/nn998
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