Abstract
The GTPase dynamin is involved in endocytosis in many cell types1, as first revealed by temperature-sensitive paralytic mutations in the Drosophila dynamin gene, shibire (shi), which disrupt synaptic vesicle endocytosis and deplete synaptic terminals of vesicles2,3,4,5,6. Here we report that shi synapses exhibit a fast synaptic fatigue phenotype within 20 ms of repetitive stimulation, which cannot be explained by vesicle depletion, as we confirmed by electron microscopy. These results suggest that, in addition to its well-characterized role in synaptic vesicle recycling, dynamin may be required for short-term maintenance of the readily releasable pool of synaptic vesicles.
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Acknowledgements
Electron microscopy was done in the Penn State Electron Microscopy facility. The shiTS2 mutant was provided by the Bloomington Stock Center. Supported by the National Science Foundation.
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Kawasaki, F., Hazen, M. & Ordway, R. Fast synaptic fatigue in shibire mutants reveals a rapid requirement for dynamin in synaptic vesicle membrane trafficking. Nat Neurosci 3, 859–860 (2000). https://doi.org/10.1038/78753
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DOI: https://doi.org/10.1038/78753
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