Brief Communication | Published:

Volitional modulation of optically recorded calcium signals during neuroprosthetic learning

Nature Neuroscience volume 17, pages 807809 (2014) | Download Citation

Abstract

Brain-machine interfaces are not only promising for neurological applications, but also powerful for investigating neuronal ensemble dynamics during learning. We trained mice to operantly control an auditory cursor using spike-related calcium signals recorded with two-photon imaging in motor and somatosensory cortex. Mice rapidly learned to modulate activity in layer 2/3 neurons, evident both across and within sessions. Learning was accompanied by modifications of firing correlations in spatially localized networks at fine scales.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    Nature 409, 403–407 (2001).

  2. 2.

    et al. PLoS Biol. 1, e42 (2003).

  3. 3.

    et al. Nature 485, 372–375 (2012).

  4. 4.

    et al. Lancet 381, 557–564 (2013).

  5. 5.

    & Trends Neurosci. 34, 61–75 (2011).

  6. 6.

    et al. PNAS 105, 19486–19491 (2008).

  7. 7.

    , , & Nat. Neurosci. 14, 662–667 (2011).

  8. 8.

    , & Neuron 79, 865–872 (2013).

  9. 9.

    , , , & Nature 483, 331–335 (2012).

  10. 10.

    , , & Front. Integr. Neurosci. 1, 6 (2007).

  11. 11.

    et al. Science 325, 621–625 (2009).

  12. 12.

    , , & Neuron 67, 1048–1061 (2010).

  13. 13.

    & Trends Neurosci. 35, 345–355 (2012).

  14. 14.

    , & J. Comp. Physiol. A Neuroethol. Sens. Neural Behav. Physiol. 192, 777–784 (2006).

  15. 15.

    et al. Nature 464, 1182–1186 (2010).

  16. 16.

    , , , & Neuron 77, 361–375 (2013).

  17. 17.

    Curr. Opin. Neurobiol. 21, 579–586 (2011).

  18. 18.

    & Nature 503, 51–58 (2013).

  19. 19.

    et al. Neuron 75, 121–132 (2012).

  20. 20.

    et al. Nature 499, 295–300 (2013).

  21. 21.

    et al. Cold Spring Harb. Protoc. 2012, 694–701 (2012).

  22. 22.

    , & Biomed. Eng. Online 2, 13 (2003).

  23. 23.

    , , , & Nat. Neurosci. 10, 1191–1197 (2007).

  24. 24.

    , & Opt. Lett. 33, 156–158 (2008).

Download references

Acknowledgements

We thank V. Jayaraman, R. Kerr, D. Kim, L. Looger and K. Svoboda for generously sharing gCaMP material, and V. Athalye and K.D. Smith for technical assistance. This work was supported by US National Institutes of Health grant 1R01NS072416-01 to D.E.F., National Science Foundation grant CBET-0954243 to J.M.C., European Research Council grant 243393 to R.M.C. and National Science Foundation Graduate Research Fellowship grant DGE 1106400 to K.B.C.

Author information

Author notes

    • Kelly B Clancy
    •  & Aaron C Koralek

    These authors contributed equally to this work.

Affiliations

  1. Biophysics Program, University of California Berkeley, Berkeley, California, USA.

    • Kelly B Clancy
  2. Helen Wills Neuroscience Institute, University of California Berkeley, Berkeley, California, USA.

    • Aaron C Koralek
    • , Daniel E Feldman
    •  & Jose M Carmena
  3. Champalimaud Neuroscience Programme, Champalimaud Center for the Unknown, Lisbon, Portugal.

    • Rui M Costa
  4. Department of Molecular & Cell Biology, University of California Berkeley, Berkeley, California, USA.

    • Daniel E Feldman
  5. Department of Electrical Engineering and Computer Sciences, University of California Berkeley, Berkeley, California, USA.

    • Jose M Carmena
  6. Joint Graduate Group in Bioengineering UCB/UCSF, University of California Berkeley, Berkeley, California, USA.

    • Jose M Carmena

Authors

  1. Search for Kelly B Clancy in:

  2. Search for Aaron C Koralek in:

  3. Search for Rui M Costa in:

  4. Search for Daniel E Feldman in:

  5. Search for Jose M Carmena in:

Contributions

K.B.C., A.C.K., R.M.C., D.E.F. and J.M.C. designed the experiments. K.B.C. and A.C.K. built the experimental apparatus, performed the experiments and analyzed the data. K.B.C. performed surgical procedures. A.C.K. and K.B.C. wrote the paper. K.B.C., A.C.K., R.M.C., D.E.F. and J.M.C. revised the paper.

Competing interests

The authors declare no competing financial interests.

Corresponding authors

Correspondence to Rui M Costa or Daniel E Feldman or Jose M Carmena.

Integrated supplementary information

Supplementary information

PDF files

  1. 1.

    Supplementary Figures

    Supplementary Figures 1–9

  2. 2.

    Supplementary Methods Checklist

    Reporting Checklist for Nature Neuroscience

Videos

  1. 1.

    Supplementary Video 1

    Imaging field from an M1-implanted animal performing the neuroprosthetic task.

  2. 2.

    Supplementary Video 2

    Imaging field from an S1-implanted animal performing the neuroprosthetic task.

About this article

Publication history

Received

Accepted

Published

DOI

https://doi.org/10.1038/nn.3712

Further reading