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Regulation of fast-spiking basket cell synapses by the chloride channel ClC-2

Nature Neuroscience volume 13, pages 10471049 (2010) | Download Citation

Abstract

Parvalbumin-expressing, fast-spiking basket cells are important for the generation of synchronous, rhythmic population activities in the hippocampus. We found that GABAA receptor–mediated synaptic inputs from murine parvalbumin-expressing basket cells were selectively modulated by the membrane voltage– and intracellular chloride–dependent chloride channel ClC-2. Our data reveal a previously unknown cell type–specific regulation of intracellular chloride homeostasis in the perisomatic region of hippocampal pyramidal neurons.

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Acknowledgements

We thank R. Zhu for excellent technical assistance, K. Ding and D. Szabadics for camera lucida reconstructions, M. Case for genotyping, J.E. Melvin for the ClC-2 knockout mouse and L. Hilgenberg, M.A. Smith, M. Cahalan, M.V. Jones, Z. Nusser and R.L. McDonald for discussions. This work was supported by US National Institutes of Health grant NS38580 (to I.S.) and the Epilepsy Foundation through the generous support of the Eric W. Lothman Training Fellowship (to S.-H.L.).

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Author notes

    • Csaba Földy

    Present address: Department of Molecular and Cellular Physiology, Stanford University, Palo Alto, California, USA.

Affiliations

  1. Department of Anatomy and Neurobiology, University of California, Irvine, California, USA.

    • Csaba Földy
    • , Sang-Hun Lee
    • , Robert J Morgan
    •  & Ivan Soltesz

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Contributions

C.F. and S.-H.L. designed the experiments. C.F., S.-H.L. and R.J.M. performed the experiments and analyzed the data. C.F. and I.S. wrote the manuscript.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Csaba Földy.

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DOI

https://doi.org/10.1038/nn.2609

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