Abstract
The hippocampus is an important brain circuit for spatial memory and the spatially selective spiking of hippocampal neuronal assemblies is thought to provide a mnemonic representation of space. We found that remembering newly learnt goal locations required NMDA receptor–dependent stabilization and enhanced reactivation of goal-related hippocampal assemblies. During spatial learning, place-related firing patterns in the CA1, but not CA3, region of the rat hippocampus were reorganized to represent new goal locations. Such reorganization did not occur when goals were marked by visual cues. The stabilization and successful retrieval of these newly acquired CA1 representations of behaviorally relevant places was NMDAR dependent and necessary for subsequent memory retention performance. Goal-related assembly patterns associated with sharp wave/ripple network oscillations, during both learning and subsequent rest periods, predicted memory performance. Together, these results suggest that the reorganization and reactivation of assembly firing patterns in the hippocampus represent the formation and expression of new spatial memory traces.
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Acknowledgements
We thank J.N.P. Rawlins and D.M. Bannerman for discussions about the behavioral procedures; J.R. Huxter and K. Allen for discussions about data analysis and the manuscript; P. Somogyi, M. Capogna, C. Lever, O. Paulsen and T. Bienvenu for comments on a previous version of the manuscript and N. Campo-Urriza for technical assistance. This work was supported by the Medical Research Council. D.D. was successively funded by fellowships from the Institut de France-Fondation Louis D. and the International Brain Research Organization (Research Fellowship), and currently holds a Junior Research Fellowship in Neurosciences from Saint Edmund Hall College, University of Oxford.
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D.D. conducted the experiments. D.D., J.O. and B.P.-B. analyzed data. D.D. and J.C. wrote the manuscript. J.C. supervised the project. All of the authors discussed the results and commented on the manuscript.
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Dupret, D., O'Neill, J., Pleydell-Bouverie, B. et al. The reorganization and reactivation of hippocampal maps predict spatial memory performance. Nat Neurosci 13, 995–1002 (2010). https://doi.org/10.1038/nn.2599
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DOI: https://doi.org/10.1038/nn.2599
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