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Acquisition frame rate affects microtubule plus-end tracking analysis

Nature Methods volume 11, pages 219–220 (2014)Cite this article

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The plusTipTracker software package originally reported by Matov et al.1,2 automates the identification, tracking and analysis of fluorescently labeled microtubule plus-end growth markers, quantifying hundreds or thousands of microtubule polymerization events in live-cell time-lapse images1,2. This algorithm infers microtubule plus-end positions during intermittent nonvisible pause and catastrophe states by spatiotemporally connecting disjointed tracks of visible plus-end labels that occur along the same microtubule vector. Pause and catastrophe states are categorized on the basis of the speed of inferred microtubule ends during the nonvisible period.

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Figure 1: Sampling frequency affects plusTipTracker results.

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Acknowledgements

This work was supported by the grant from the US National Institutes of Health (R01NS064288) to F.-Q.Z.

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  1. fzhou4@jhmi.edu

Authors and Affiliations

  1. Department of Orthopaedic Surgery, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

    Philip R Nicovich & Feng-Quan Zhou

  2. Biomedical Imaging Facility, Mark Wainwright Analytical Centre, University of New South Wales, Sydney, New South Wales, Australia

    Philip R Nicovich

  3. The Solomon H. Snyder Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

    Feng-Quan Zhou

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  1. Philip R Nicovich
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  2. Feng-Quan Zhou
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Correspondence to Philip R Nicovich or Feng-Quan Zhou.

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The authors declare no competing financial interests.

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Supplementary Figures 1–3, Supplementary Table 1 and Supplementary Methods (PDF 492 kb)

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Nicovich, P., Zhou, FQ. Acquisition frame rate affects microtubule plus-end tracking analysis. Nat Methods 11, 219–220 (2014). https://doi.org/10.1038/nmeth.2846

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