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Total internal reflection microscopy is a light microscopy technique that relies on total internal reflection of illumination light to generate an evanescent wave that extends less than 200 nm from the microscope coverslip. This allows selective excitation of fluorophores immediately adjacent to the coverslip or monitoring of the distance between the coverslip and a microscopic sphere.
Holographic microscopy with independent control of the signal and reference fields enables the holographic imaging of a single protein with mass below 100 kDa and estimation of their polarizability.
Here, the authors elucidate how sequential activities of opposite-polarity microtubule motors are coordinated on a cargo, by showing how the Tm1 protein inhibits kinesin-1 during delivery of oskar RNA into the Drosophila oocyte by dynein.
Native-nanoBleach, a single-molecule imaging technique with a spatial resolution of ~10 nm, quantifies the oligomeric distribution of membrane proteins directly from native membranes at endogenous expression levels with their proximal native membrane environment using amphipathic copolymer nanodiscs.
Integration of curved GaAs metagratings onto VCSELs enables a compact on-chip illumination solution for high-contrast microscopy, making it a versatile choice for light sources in lab-on-a-chip devices.
Single-particle tracking (SPT) has revolutionised studies of protein interactions but is often limited by photobleaching. Here, the authors evolve DNA-PAINT-SPT to enable simultaneous dual-colour detection for the quantification of protein dimerization and live cell membrane protein tracking.
GABAB receptors mediate the effects of the main inhibitory neurotransmitter in the brain. Here, authors identify the cytoskeletal protein filamin A as a key player that controls the exact location and function of GABAB receptors at the cell surface.
Researchers use an in vitro assay in combination with a polymer model to study protein–DNA condensation and how condensates bring distant DNA elements into close proximity.
