Stem cell–based regenerative medicine is a promising approach in tissue reconstruction. Here we show that proinflammatory T cells inhibit the ability of exogenously added bone marrow mesenchymal stem cells (BMMSCs) to mediate bone repair. This inhibition is due to interferon γ (IFN-γ)–induced downregulation of the runt-related transcription factor 2 (Runx-2) pathway and enhancement of tumor necrosis factor α (TNF-α) signaling in the stem cells. We also found that, through inhibition of nuclear factor κB (NF-κB), TNF-α converts the signaling of the IFN-γ–activated, nonapoptotic form of TNF receptor superfamily member 6 (Fas) in BMMSCs to a caspase 3– and caspase 8–associated proapoptotic cascade, resulting in the apoptosis of these cells. Conversely, reduction of IFN-γ and TNF-α concentrations by systemic infusion of Foxp3+ regulatory T cells, or by local administration of aspirin, markedly improved BMMSC-based bone regeneration and calvarial defect repair in C57BL/6 mice. These data collectively show a previously unrecognized role of recipient T cells in BMMSC-based tissue engineering.
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We thank X. Duan and T. Zhou of the Fourth Military Medical University for generating microCT images. This work was supported by grants from the US National Institute of Dental and Craniofacial Research, US National Institutes of Health, Department of Health and Human Services (R01DE017449, R01DE019932 and R01DE019413 to S.S.), a grant from California Institute for Regenerative Medicine (RN1-00572 to S.S.) and the Intramural Program of the US National Institute of Dental and Craniofacial Research, US National Institutes of Health, Department of Health and Human Services.
The authors declare no competing financial interests.
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