Date palms (Phoenix dactylifera) are the most significant perennial crop in arid regions of the Middle East and North Africa. Here, we present a comprehensive catalogue of approximately seven million single nucleotide polymorphisms in date palms based on whole genome re-sequencing of a collection of 62 cultivars. Population structure analysis indicates a major genetic divide between North Africa and the Middle East/South Asian date palms, with evidence of admixture in cultivars from Egypt and Sudan. Genome-wide scans for selection suggest at least 56 genomic regions associated with selective sweeps that may underlie geographic adaptation. We report candidate mutations for trait variation, including nonsense polymorphisms and presence/absence variation in gene content in pathways for key agronomic traits. We also identify a copia-like retrotransposon insertion polymorphism in the R2R3 myb-like orthologue of the oil palm virescens gene associated with fruit colour variation. This analysis documents patterns of post-domestication diversification and provides a genomic resource for this economically important perennial tree crop.
The last decade has been an intensive time for studying the genetic basis of crop domestication and diversification1,2,3. Much of the work has focused on annual crop species1,3, particularly the cereal crops where patterns of genome diversity have been characterized. Genomic resources in annual crops, such as development of single nucleotide polymorphism (SNP) maps of genomes, have led to the identification of numerous loci underlying phenotypic diversity4,5. In contrast, there has been relatively little attention paid to perennial crops, including fruit tree species. Perennial crops, given their diverse life histories and breeding systems, are likely to evolve in distinct ways from annual crop species6. Moreover, the availability of genomic resources in these long-lived crop taxa will be necessary if we are to fully exploit phenotypic diversity and accelerate breeding to improve global food security, particularly in tree species7.
The date palm (Phoenix dactylifera L.) is a dioecious, perennial diploid (2n=36) tree in the Arecaceae family and is the most important fruit-bearing crop in arid regions of the Middle East and North Africa8,9,10. Date palms grow primarily in hot, arid habitats including desert oases, river valleys and well-irrigated farms or plantations. Individual varieties are valued primarily for fruit-related traits including moisture and sugar content, and as many as 3,000 varieties are recognized worldwide10. The recent completion of two assemblies of the 690 Mb genome of the Khalas variety by two independent groups11,12 has enabled new discoveries, including identification of the sex determination locus11 and characterization of pathways active during fruit maturation12. Both assemblies are presently in the draft stage with the more complete assembly12 consisting of 82,354 scaffolds with an N50 of 329.9 kb and a maximum scaffold size of ∼4.5 Mb. This assembled sequence covers 90.2% of the genome and contains 41,660 gene models.
The origin of P. dactylifera remains enigmatic, and no clear wild ancestor has been identified9. The sugar palm, P. sylvestris, has been considered to be a likely progenitor of domesticated date palm. This species currently grows wild in the Indus Valley and parts of India, and its historical range may have extended as far west as the Gulf region13. However, genetic data have not established a clear relationship between P. dactylifera and P. sylvestris, or any of several peri-patrically distributed species with which it is known to hybridize14. Recent work suggests the origin of domesticated dates to be in the Middle East15,16, which is supported by archaeological data in the region15,16,17. The Gulf region holds the oldest evidence of date palm exploitation, with seeds dating to 7100 BP excavated in site remains in Dalma Island, Abu Dhabi and Kuwait15,16,17. Although alternate hypotheses propose a domestication centre of date palm in North Africa18, this crop appears ∼3,000 years later in the archaeological records of this region19.
Domesticated date palms exhibit a wide range of phenotypic diversity in fruit colour, sugar content, flowering time and other agronomic traits. Much of this diversity is likely the result of evolutionary diversification that drove adaptation of date palm cultivars across its native range in Asia and Africa. Characterization of genome diversity in date palm cultivars and discovery of genes controlling traits of interest will improve the prospects of date palm breeding for yield and other agronomic traits while providing a means to answer long-standing questions about date palm diversity and the history of domestication.
Despite the central importance of date palm as a traditional crop in the Middle East and North Africa and the agronomic challenges to their continued sustainable cultivation, very little is known about the genomic diversity of this species. In this article, we present analyses of whole genome re-sequencing of 62 varieties of P. dactylifera. The sequenced cultivars originate from locations spanning the traditional range of date palm cultivation from North Africa to the Middle East, as well as newer production areas in Pakistan. Our samples include common commercial varieties from all of the major date-producing countries in the region, and our analysis provides the first comprehensive catalogue of molecular variation in this species.
Variation in the date palm genome
We sequenced the genomes of 62 varieties of date palm from 12 countries spanning the traditional range of cultivation of this species. Seventeen of the cultivars are from Africa (North Africa, Egypt and Sudan), 36 from the Middle East (the Arabian peninsula, Iraq and Iran) and nine from South Asia (Pakistan; Supplementary Table 1), with the largest sample of 17 varieties in our data set originating from Iraq. Approximately, 10 of the samples are popular varieties that are commercially important and cultivated widely and, include Medjool, Deglet Noor, Barhee and Zahidi. The rest are grown in more restricted areas and in smaller numbers. Although date palms are primarily propagated vegetatively, many of the cultivars may be landraces20 and have not been subjected to scientific breeding.
Using paired-end (2 × 100 bp) Illumina sequencing, we obtained a mean sequencing depth of 20.8 × per sample when aligned to the cv. Khalas reference genome12 (Supplementary Table 2). After aligning the re-sequencing reads, we processed the alignments to remove duplicate reads, realigned reads around indels21,22, and applied a series of quality control filters with the intent of limiting false-positive variants23 (see Methods section). This procedure yielded 7,176,238 SNPs (excluding those found in transposable elements (TEs)), or ∼12 SNPs per kb, representing the most common sequence polymorphisms in cultivated date palm (Fig. 1a). An additional 4,933,882 SNPs are found in repetitive sequences, which we annotated as TE sequences24, and are excluded from our final SNP call set. To evaluate the quality of SNPs, we examined 37 genotypes by PCR amplification and Sanger sequencing and confirmed 36 of 37 (Supplementary Table 3), which represents a concordance rate of 97.2%.
The majority of non-TE SNPs in date palms (5,222,681 SNPs or 72.77%) are located in intergenic regions (Fig. 1b). SNPs in genic regions excluding TEs include 201,160 synonymous, 234,740 nonsynonymous (Fig. 1b), 1,479,953 intronic and 7,811 and 17,622 SNPs in 5′ and 3′ UTRs, respectively. The site-frequency spectrum for the various functional SNP classes indicates that both nonsynonymous and nonsense polymorphisms are skewed towards lower frequencies, suggesting they are enriched for slightly deleterious mutations (Fig. 1c).
We estimate the population mutation parameter defined as Watterson’s theta (θW) and nucleotide diversity (π) to be 0.01000±0.00003 and 0.0092±0.00002 (±represents standard error [s.e.], n=62), respectively (Supplementary Table 4). This indicates that pairs of randomly selected sequences on average differ at ∼1% of nucleotide sites. This estimate of nucleotide variation in date palm is higher than those for other perennial fruit crops, such as cassava (π=0.0026)25, peach (π=0.0015)26 and grapevine (π=0.0051)27.
Linkage disequilibrium (LD) decays relatively rapidly in date palms. LD measured as the squared correlation coefficient (r2) between SNPs decays to ∼50% of its maximum at ∼6 kb and 90% of its maximum at ∼40 kb (Fig. 1d). The majority of SNP pairs with r2 in near complete disequilibrium (>0.8) are found at physical distances less than 10 kb (Supplementary Fig. 1). This relatively rapid decay of LD suggests that genome-wide association studies (GWASs) should enable high-resolution mapping of genes associated with traits of agricultural significance.
Population structure of date palms
GWA mapping studies and modern breeding strategies, benefit from an understanding of population structure, LD and the genome-wide distribution of genetic variation28. Previous studies have suggested population differentiation within domesticated date palm29,30, and our genome-wide analysis confirms the presence of distinct North African and Middle Eastern/South Asian populations. This subdivision is apparent in the first axis of a principal component (PC) analysis31 (Fig. 2a) and in a neighbour-joining tree32 based on genetic distances from the whole genome SNP data (Fig. 2b). Using the population-based clustering method STRUCTURE33, we fit a model of population stratification in which an individual’s genome is inferred to be composed of sites from up to K ancestral populations. We ran STRUCTURE for K=1–10 using the admixture model with correlated allele frequencies. Application of the Evanno method34 indicates that K=2 has the highest ΔK and therefore represents the best fit to the data (Fig. 2c; Supplementary Table 5), which is consistent with the distance-based and PC results. In the STRUCTURE analysis, the Middle Eastern and South Asian cultivars form a separate group, while the North African cultivars have a majority contribution of their genome from a population distinct from that found in the Middle East (Fig. 2c).
Our analysis provides evidence of genetic admixture between the predominant North African and Middle East populations in multiple varieties (Fig. 2c) consistent with hybridization between these two populations. The extent of admixture between the two regional populations ranges between <1 and 99% (Fig. 2c), with a number of samples appearing to be admixed between western and eastern subpopulations. Samples from the geographically intermediate areas of Egypt and Sudan are among the most admixed samples with 55–65% of their genomes being derived from the Middle Eastern population in the STRUCTURE analysis and appear as intermediate on the first axis of the PC analysis and a neighbour-joining tree. Admixture in samples from Egypt is consistent with a previous report30 and may indicate a hybrid origin of varieties cultivated in areas where the two subpopulations come into contact. Admixed samples from outside this region are also apparent (Fig. 2c), and may represent recent transfer of cultivars away from their country of origin30.
The North African population (excluding admixed samples such as those from Egypt and Sudan) has higher levels of nucleotide diversity compared with the Middle Eastern/South Asian population (πNorth Africa=0.01080±0.00003; ±s.e., n=10 versus πMiddle East/South Asia=0.00810±0.00002; ± s.e., n=42; Supplementary Table 4). Higher diversity in Africa is also supported by estimates of θ from single diploid genomes35, which provides a more granular view of how diversity varies across the range of P. dactylifera. With one exception, all of the highest nucleotide diversity estimates from single genomes are from African samples (Supplementary Table 6), while single genome estimates outside of Africa have consistently lower levels of genetic diversity. These observations suggest that North African varieties are derived from a distinct North African population with a larger effective size (Ne) than the Middle Eastern population.
Genomic evidence of inbreeding in date palms
Date palms are obligate outcrossers, although cultivars show varying degrees of inbreeding. The individual inbreeding coefficient Find in different varieties ranges from completely outcrossed (Find=1.2 × 10−5) to mildly inbred (Find=0.125; Supplementary Table 6). More inbred cultivars such as cv. Ajwa and cv. Medjool have a large percentage (∼25%) of genomic segments that are almost completely homozygous, reflected in the bimodality in the distribution of heterozygosity in single genomes, with a peak near 0% heterozygosity (Fig. 3a). In contrast, outbred samples such as cv. Fagous and cv. Mazafati, have a more uniform genome-wide distribution, a higher average over-all heterozygosity, and relatively few genomic intervals that are predominantly homozygous (Fig. 3a).
In the most inbred samples, homozygous intervals are frequently found in long (spanning >500 kb) runs of homozygosity (ROH) with few heterozygous genotypes. This is evident in genomic regions where the percentage of heterozygous genotypes is reduced to approximately zero (Fig. 3b; Supplementary Fig. 2). In some cases, ROH are found in tracts spanning the length of the longest scaffolds in the genome assembly and therefore can exceed 1 Mb (Supplementary Fig. 2). Inferring ROH lengths is constrained by the quality of the draft assembly12, but the presence of long ROH implies that homozygous regions may be prominent features of some cultivars. This pattern of identity-by-descent may reflect a history of inbreeding in some varieties due to differences in the intensity of breeding, or conscious selection, for desired traits.
Geographic selection in date palms
Strong population subdivision between North African and Middle Eastern/South Asian populations provides an opportunity to find genes associated with geographic adaptation by identifying genomic regions with reduced levels of nucleotide diversity in one population compared with the other36,37,38. We separately estimated θW in 5 kb windows for varieties from Africa and from the Middle East/South Asia, and for each interval calculated the ratio of diversity in the two populations.
We constructed a empirical distribution of this ratio (Supplementary Fig. 3), and identified outliers in the tails of a Z-score transformed distribution as regions that may harbour candidate adaptive genes36,37,38. By using this method, and applying the additional criteria that the Z-scores for two 5-kb windows within 10 kb of each other had to both be in the tail of the distribution (see Methods section), we identified ∼36 genomic regions that may have been subject to positive selection in the Middle East, and 20 regions in North Africa (Supplementary Tables 7 and 8). The sizes of these putative selective sweep regions range from ∼10–70 kb. We also estimated Tajima’s D for each of these 5-kb windows across the genome, and find that genomic regions with outlier Z-scores 3 or more s.d. from the mean are enriched for negative Tajima’s D value, consistent with recent selective sweeps in these regions (χ2 test, P<0.0001 for Middle East and P<0.0175 for North Africa; Supplementary Table 9).
As an example, one outlier region on scaffold S000007 is depleted of nucleotide variation and has a ∼93% reduction in nucleotide diversity in Middle Eastern varieties relative to North African cultivars (θNorth Africa=0.01027 versus θMiddle East=0.00077, Z=−8.088)5. Consistent with a history of recent positive selection, this region has a site-frequency spectrum skewed towards low frequency alleles (Tajima’s D=−2.2780) in Middle Eastern/South Asian varieties, which is in the lower 0.1% of values in a genome-wide scan. This area of low diversity spans ∼10 kb, and includes a gene (KacstDP.mRNA.S000007.21) that encodes a pectin lyase (Fig. 4). This gene is implicated in cell wall degradation and fruit softening39, and points to geographic selection for differing fruit ripening characteristics.
Dates can be classified as being dry, semi-dry or soft when ripe. To examine whether fruit texture has a geographic association consistent with the selective sweep at the pectin lyase locus, we compiled published data on the fruit characteristics of 107 date varieties8,40. We find that North African varieties (n=64) are more variable in texture, with ∼52% of varieties producing soft dates and ∼31% dry. In contrast, a sample of Middle Eastern and South Asian date varieties (n=43) indicated that varieties producing soft dates account for ∼77% of the sample while dry date-producing varieties account for ∼7%. This predominance of soft dates in Middle Eastern/South Asian varieties is statistically significant (Fisher’s exact test, P<0.0026), and is consistent with selection at the pectin lyase locus in Middle Eastern/South Asian varieties associated with cultural selection for fruit texture. Further studies can provide clearer mechanistic connections between specific mutations at this locus and fruit characteristics.
Candidate loss-of-function polymorphisms
Major effect mutations, including gene deletions and nonsense polymorphisms that pre-maturely truncate encoded proteins, are classes of mutation predicted to impair gene function and represent the best candidates for functional variation among cultivars. We characterized candidate mutations in pathways associated with important agronomic traits including disease resistance, fruit ripening, fruit colour, flowering time and sugar metabolism (Fig. 5a). In total, we discovered 4,162 nonsense polymorphisms affecting 3,288 genes (excluding TE-related genes). Of these genes, 2,720 (65%) truncate the protein by at least 25% and are thus good candidates for loss-of-function alleles. Predicted major effect mutations including splice site and nonsense polymorphisms are dependent on the quality of the genome annotation and lower quality annotations are expected to inflate the number of genes impacted by these classes of damaging mutation. A comparable study of Arabidopsis thaliana, which has a well-curated structural annotation, found more than 4,000 genes affected by nonsense mutations in a set of 80 re-sequenced accessions41, which is comparable to the number in date palm.
In addition to characterizing major effect classes of SNPs, we identified cases of presence/absence variation in gene content among cultivars. By using a coverage criterion (see Methods section) to characterize gene deletions, we predicted 1,402 gene loss events. Many of these gene losses (27%) were found in only one cultivar, while ∼25% were found at moderate to high frequency (>20% of cultivars).
Both nonsense polymorphisms and gene deletions segregate in members of almost all large gene families and functional pathways we examined (Fig. 5a; Supplementary Data 1 for genes assigned to pathways). We find that only a small fraction of candidate flowering time (8%), sugar metabolism (13%) and fruit ripening (10%) genes are segregating for putative nonsense or splice site mutations that could affect gene function (Fig. 5a). Moreover, large gene families in the date palm genome including helix-loop-helix and myb-like DNA-binding proteins, cytochrome P450s and protein kinases, have putative nonsense mutations in ∼13% of the genes in these families (Fig. 5a). The fraction of observed gene losses is smaller, with a mean of ∼1.4% of genes segregating for a deletion in these families.
Variation in candidate disease resistance genes
Disease resistance is an important agronomic trait in date palms where crop yields have been devastated by pathogens including the fungus Fusarium oxysporum responsible for Bayoud disease42. Resistance genes including the nucleotide-binding site/leucine rich repeat (NBS–LRR)43, receptor-like kinases (RLKs)44 and receptor-like proteases (RLPs)45 are conserved members of the plant innate immunity system and represent excellent candidates for variation in susceptibility to disease observed in date palm46. We evaluated if these conserved gene classes show evolutionary genetic patterns consistent with their presumed role in the immune response. Regions with CC–NBS–LRR (24 genes) and NBS–LRR genes (95 genes) are more polymorphic than other genomic regions (Fig. 5b; PCC–NBS–LRR<1.0 × 10−16; PNBS–LRR<1.0 × 10−21), consistent with long-term balancing selection acting on pathogen resistance loci as reported in other plants (Fig. 5b)47. RLPs, while not as diverse as the NBS–LRR containing proteins, also show significantly elevated nucleotide diversity (P<1.0 × 10−7), while RLKs are not found in unusually polymorphic regions of the date palm genome (Fig. 5b).
In addition to elevated levels of diversity, all classes of date palm resistance genes (with the exception of the NBS–LRR class) have a significant skew in the site-frequency spectrum towards higher frequency alleles as evidenced by more positive Tajima’s D estimates compared with the genome-wide mean of D=−0.272 (Tajima’s DNBS–LRR=−0.291 (P=0.545); DCC–NBS–LRR=−0.003 (P=0.00733); DRLK=−0.145 (P=0.00019); DRLP=0.0003 (P=4.7 × 10−5); Supplementary Fig. 4). Moreover, date palm varieties in our sample are frequently polymorphic for gene content in these gene classes (Fig. 5c). Together, these observations suggest that these classes of genes are evolving in a manner similar to what has been observed in disease resistance genes in other plant species, and are therefore candidates for variety-specific disease resistance reported in P. dactylifera46.
Fruit colour polymorphism and the virescens gene
Date palm varieties are valued for a diversity of fruit characteristics including colour, sugar composition, and texture. Fruit colour is central to varietal identity in date palms where khalal stage fruit varies in colour from dark red to light yellow (Fig. 6a). In the related oil palm Elaeis guineensis, the red and yellow fruit colour polymorphism is controlled by the virescens (VIR) gene, which encodes an R2R3 myb-like transcription factor48. Nonsense alleles of oil palm VIR act as dominant negative mutations that suppress the production of anthocyanins present in the epicarp of red fruit and thus leads to a yellow-coloured fruit.
We identified the date palm ortholog of VIR and present phylogenetic analysis that indicates it is most closely related to the VIR gene in oil palm (Fig. 6b). Interestingly, we observed that the VIR allele present in the cv. Khalas reference genome (NCBI Gene ID LOC103717680) has a copia-like long terminal repeat (LTR) retrotransposon insertion in the third exon, which truncates the VIRcopia allele relative to the oil palm gene (Fig. 6c,d; Supplementary Fig. 5). This is intriguing as cv. Khalas has yellow fruit and truncated alleles of VIR are responsible for yellow fruit colour in oil palm48.
By using a combination of approaches, we successfully designed PCR primers and amplified across the copia-like insertion and confirmed the existence of a 397-bp insertion in the cDNA isolated from the retrotransposon insertion allele VIRcopia of yellow-fruited cultivars. (Fig. 6d). This VIRcopia allele contains a T169* mutation located near the 5′ end of the copia LTR insertion, which truncates the wild-type (VIR+) protein by 62 amino acids (∼30% of the protein) that spans a transcriptional activation domain of the R2R3 myb-like transcription factor. By using a similar PCR-based approach, we successfully sequenced a complete VIR+ allele from both cDNA and genomic DNA from a cultivar with red fruit. VIR+ contains an open reading frame that is homologous to wild-type VIR in oil palm and includes an intact exon 3 (Fig. 6c,d).
We genotyped the data palm VIR orthologue in 36 varieties for which we had fruit colour information (Supplementary Table 10). We find that varieties that produce red fruit are found exclusively as VIR+/VIR+ homozygotes (n=8), while yellow-fruited varieties are either heterozygous (VIR+/VIRcopia; n=10) or homozygous (VIRcopia/VIRcopia; n=18) for the copia-like insertion allele (P<5.0 × 10−7; Fig. 6e). This association between genotype at VIR and fruit colour phenotype suggests that VIR controls fruit colour in date palms and the pattern of dominance is consistent with VIRcopia acting as a dominant negative inhibitor of anthocyanin production comparable to the nonsense alleles reported in oil palm48. The genetic pattern we observe in date palm therefore parallels what has been observed in oil palms, where VIR acts monogenically to control fruit colour. Together, these results provide evidence that we have likely identified a causal allele for fruit colour polymorphism in date palms, and that yellow fruit colour in date and oil palms represents a genetic parallelism in this trait.
We report a comprehensive catalogue of genome-wide polymorphism in date palms, one of the most culturally and economically important crops of the Middle East and North Africa. The re-sequencing data provides essential information on the population structure, evolutionary history, and diversification of P. dactylifera, offers markers for varietal identification and GWAS approaches to mapping agriculturally important genes, and yields candidate mutations that present new opportunities for crop improvement.
Discovery of extensive variation within date palms and evidence of genetic differentiation between regional populations will be an important factor in structured association mapping28, as well as planning future breeding programs that capture the full range of diversity in date palm. In addition to discovery of extensive diversity, the relative rapid decay of LD in date palms suggests that GWAS should enable high-resolution mapping of genes associated with natural phenotypic variation. Interestingly, there is evidence for greater levels of inbreeding in some date palm cultivars despite the outcrossing nature of this species, which may allow for homozygosity mapping of recessive genes49,50.
Our finding that North African and Middle Eastern/South Asian date palm varieties are genetically distinct, as well as the late appearance of date palms in the North African archaeological data18,19, may indicate that date palms were domesticated in the Middle East with the rise of oasis agriculture15,16,17 and subsequently spread westward. We find, however, higher nucleotide diversity in North Africa, suggesting that this population is not the product of recent colonization and founder bottleneck following a hypothesized spread of date palms from a centre of origin in the Middle East.
We are unable to establish a clear understanding of the domestication history of date palms given that wild ancestral populations have never been identified14. Nevertheless, the evidence of geographic structure between regional North African and Middle Eastern populations coupled with the known archaeological record can be explained in two ways. One is that this species has arisen from two domestication events, one in the Middle East and a second late domestication in North Africa, possibly from separate gene pools that had diverged before the onset of domestication in either locale. Alternatively, one can posit the spread of domesticated Middle Eastern date palms and subsequent introgression with a wild or semi-cultivated population in North Africa. The latter is consistent with the elevated diversity of North African date palms, as well as the late appearance of dates in the archaeological record of the region; further detailed evolutionary demographic analysis may help discriminate between these alternative scenarios.
This catalogue of polymorphism provides a foundational resource that will assist with addressing challenges faced in date palm agriculture and developing hypotheses for genotype/phenotype relationships for trait diversity. For example, we have identified possible selective sweeps within P. dactylifera that promote geographic diversification. Fifty-six regions of the genome show a pattern consistent with independent histories of selection in African and Middle Eastern populations. Several of these regions harbour genes associated with fruit traits or response to abiotic stress, and provide material for further genetic studies of phenotypic diversification in this crop.
Aside from documenting the nature of perennial crop diversification, this study offers the possibility of marker-assisted selection, targeted breeding for specific traits, and discovery of candidate mutations in pathways associated with important agronomic traits7. Evidence that fruit colour variation in date and oil palms arise from mutations in the same gene suggests that, despite the evolutionary divergence between these two species, it may be possible to share genetic and molecular information across them to facilitate crop improvement. The availability of genome-wide SNPs, and the growing availability of comparative genome resources in plants, provides the ability to accelerate efforts to both uncover the nature of genetic and phenotypic diversity, but also to apply this to agronomic improvement in date palm7.
Sample collection and library preparation
Samples were obtained from either leaf or fruit tissue from 61 female and 1 male (cv. Fard4) cultivar of P. dactylifera from sources around the Middle East, North Africa, South Asia and the United States (Supplementary Table 1). Samples from the United States have a well-documented history of transplanting from locations in North Africa and the Middle East and their origins can therefore be traced to their original source8 country listed in Supplementary Table 1. DNA was extracted using plant DNeasy mini kit (Qiagen, Venlo, Netherlands) DNA extraction columns, and libraries prepared using Illumina TruSeq or Nextera (San Diego, CA) kits; 2 × 100 paired-end sequencing was conducted on an Illumina HiSeq 2500 sequencer (San Diego, CA) at the Center for Genomics and Systems Biology, New York University Abu Dhabi with one to four libraries per lane.
Read alignment and SNP calling
Reads passing Illumina’s quality control filter were aligned using Burroughs-Wheeler Aligner (v0.6.2)51 to the cv. Khalas reference genome12 that was modified to include the 158,462 bp chloroplast genome (Genbank GU811709.2)52. This modified assembly consisted of 558,181,296 bp in 82,355 scaffolds. Alignments for each sample were processed by removing duplicate reads using Picard-tools (version 1.82) MarkDuplicates and then merged using Picard-tools MergeSamFiles. Reads in insertion/deletion (indel) regions were then globally realigned using the Genome Analysis Toolkit (GATK) RealignerTargetCreator/IndelRealigner protocol (version 2.7–4)21,22.
SNP calling was performed using the GATK Unified Genotyper configured for diploid genomes. Sequencing reads with mapping quality of zero and low base quality were filtered before SNP calling per the GATK default settings. Base qualities were capped at the mapping quality of the read and bases close to indels adjusted downward during the SNP-calling step using the base alignment quality method to reduce false positives near indels53. Improperly paired reads were removed by applying the BadMateFilter per the Unified Genotyper default settings. SNPs were filtered to remove variants with total depth across samples of <345 (that is, less than an average of 5 × per sample) and depth >2,836 (that is, approximately twice the mean depth at variant sites), those with more than 5% missing genotypes and those found in repetitive sequences identified as low-complexity regions23 by mdust (ftp://occams.dfci.harvard.edu/pub/bio/tgi/software/seqclean/) or as TEs by REPET24. Three additional filters (MQRankSum<−42, ReadPosRankSum<−10, BaseQRankSum<−7)21,22 were applied based on low transition/transversion ratio suggesting enrichment for false positives below the cutoff thresholds.
SNP effects were assigned to each SNP using snpEff (version 2.0.5)54 based on gene models from the cv. Khalas reference genome12 and multiple effects collapsed to the most damaging effect using GATK VariantAnnotator21,22. Codons with two or three SNPs in the same codon were annotated separately as multiple-substitution codons and not considered further owing to potential mis-assignment of the effect by snpEff. All SNP effect classifications are dependent on published date palm gene models12 and future refinement of gene models may alter predicted effects. Raw read alignments and SNP/Indel polymorphisms in the re-sequenced genomes can be visualized in JBrowse55 at http://jbrowsephoenix.abudhabi.nyu.edu.
Homozygous gene deletions were inferred using a coverage breadth criterion. Gene models in which one or more samples were covered by at least one read at fewer than 15% of sites were considered as candidates for gene deletions. To reduce false positives associated with regions refractory to read mapping, inferred gene deletions were also required to be covered at 90% or more of sites in at least one re-sequenced sample. Samples with lower than 15 × coverage (Supplementary Table 2) were excluded from the gene deletion analysis.
Gene expression in fruit
RNA-seq data from fruit was used to confirm that candidate genes (for example, VIR) are actively transcribed in the fruit. Gene expression data (FPKM) from fruit was obtained by mapping publicly available RNA-seq data56 to the reference assembly with Tophat (v2.0.6) using the -G and --no-novel-juncs arguments57. Read counts for each genome feature were determined using HTseq (v0.5.3p9; http://www-huber.embl.de/users/anders/HTSeq).
PC analysis31 and STRUCTURE33 analyses were conducted on a reduced SNP data set. Given that most scaffolds in the draft assembly12 have unknown physical locations and the linkage relationships of most SNPs are therefore unknown, we randomly selected ∼25 000 SNPs to reduce the impact of LD on the clustering results33. PC was conducted on the reduced data set with SNPRelate31.
STRUCTURE (version 2.3.4)33 was implemented using the admixture and no linkage models with a burnin length of 100,000 and 200,000 MCMC replicates following the burnin phase. The analysis was repeated 14 times for each value of K=1–10 and the program CLUMPP (version 1.1.2)58 used to permute the clusters generated from independent STRUCTURE runs. Analysis was run with the admixture model allowing for correlated allele frequencies among populations (Supplementary Table 5). K=2 was chosen as the best fit for the data based on a higher ΔK34 as calculated by Structure Harvester (v0.6.93)59.
Genetic distance estimation
Neighbour-joining trees were constructed for the 62 P. dactylifera genome sequences using sites passing quality control filters (see above). Distances were calculated using a custom perl script that applies the following equation60 to the filtered whole genome SNP data:
where X and Y represent two sample genomes, L is the total number of variable (that is, SNP) sites, ai and bi are the two allele copies in sample X, ci and di are the two alleles copies in sample Y. Trees were then constructed from the distance matrix using the neighbour-joining method implemented in MEGA v6.06 (ref. 61).
Population genetic parameter estimation
Population genetic statistics θW, π, and Tajima’s D were calculated directly from short-read alignments using ANGSD with non-overlapping 10 kb intervals (version 0.609)62. Regional estimates of θW and π are based African (n=10) or Middle Eastern (n=42) samples with little or no evidence of admixture in the STRUCTURE33 analysis. Single genome-based estimates of the population mutation rate θ were obtained directly from each of the sample alignments using mlRho (version 2.7)35; (Supplementary Table 6). Per-individual inbreeding coefficients were estimated using ngsF63 with genotype likelihoods generated by ANGSD62 provided as input. Differences in θW and Tajima’s D between genomic intervals with and without R genes were tested with a two-tailed Wilcoxon rank-sum test. FST was calculated for non-overlapping genomic intervals in 500 bp windows using pypgen version 0.2.1 (https://pypi.python.org/pypi/pypgen).
LD was calculated using VCFtools (version 0.1.13)64 with the settings—geno-r2—ld-window-bp 100000—maf 0.1 to exclude SNPs with minor allele frequency <10%. The genome-wide LD decay rate (Fig. 1d) was estimated by binning r2 values in 1 kb intervals based on the physical distance between SNPs in the reference assembly and calculating the mean r2 for each interval. The analysis presented in Supplementary Fig. 1 was conducted using the same LD outputs as above, but binning r2 values into five categories based on the extent of LD. The cumulative count of SNP pairs in each category was then plotted as a function of the physical distance between SNPs in each pair. All LD analyses are based on the 390 scaffolds in the genome assembly that exceed 100 kb in length.
Analysis of candidate selective sweep regions
Nucleotide diversity across the genome varies due to a combination of variation in the neutral mutation rate, the effects of linked selection and stochasticity of the coalescent process. Distinguishing neutral from selective processes responsible for patterns of polymorphism is challenging and increasingly so without an appropriate outgroup. We therefore adopted a comparative approach to identify regions of the genome where one of the two subpopulations identified by STRUCTURE33 is depleted of nucleotide variation with respect to the other, which may indicate the presence of a selective sweep in one population. Outlier regions were identified using this empirical distribution approach by calculating the log-transformed ratio of θW estimated for African (n=10) and Middle Eastern (n=42) for each 5 kb interval in the genome. The log-transformed ratios were then Z-score transformed so that differences in θW between geographic regions can be compared across genomic intervals with respect to s.d. from the mean as follows:
where xi is log2(θpopulation1/θpopulation2) in each genome interval and s.d.(x) is the s.d. of x. The lower half of the Z-score distribution was then evaluated for both θMiddle East/θAfrica and θAfrica/θMiddle East. Values in the lower tail of each distribution and at least five s.d. from the mean were considered outlier regions and candidates for selective sweeps.
Heterozygosity in individual samples
The numbers of homozygous and heterozygous genotypes per sample were counted in 20 and 50 kb intervals on scaffolds with at least one complete interval. The proportion of heterozygous genotypes (that is, number heterozygote genotypes/total genotype calls) were then tabulated and density distributions and heatmaps constructed using ggplot2 (http://ggplot2.org). ROH were identified manually as extended regions (>500 kb) with zero, or near zero, heterozygosity in samples with at least 15 × coverage.
Gene functional annotation and gene set analysis
Genes were annotated with PFAM terms using InterProScan 5 (http://www.ebi.ac.uk/Tools/pfa/iprscan5/), Gene Ontology (GO) terms using AutoFact65 and Kyoto Encyclopedia of Genes and Genomes (KEGG) identifiers using the KEGG Automated Annotation Server (KAAS; http://www.genome.jp/tools/kaas/). Sugar metabolism and fruit ripening gene classes were identified based on a combination of InterProScan 5 (http://www.ebi.ac.uk/Tools/pfa/iprscan5/) and homology to A. thaliana genes using Proteinortho V5.11 (ref. 66; (Supplementary Data 1). Candidate resistance R genes were identified using the PRGdb database 2.0 (ref. 67). Candidate flowering-time loci were obtained based on homology to flowering-time genes in A. thaliana68 using Proteinortho V5.11 (ref. 66). Statistical analysis was conducted in the R Statistical Programming Language (http://www.R-project.org).
Repeats identification and annotation
Identification and annotation of repeats was performed with the REPET package24 on the cv. Khalas genome assembly.
Characterization of VIR
The orthologue to the oil palm R2R3 myb-like transcription factor encoded by the VIR locus was determined using proteinortho V5.11 (ref. 66) and was found to encode a conserved protein that is expressed in date palm fruit56. Alignment of the orthologous protein (NCBI Gene ID LOC103717680) on scaffold S000271 (ref. 12) to the orthologous oil palm sequence and other R2R3 myb-like transcription factors (Supplementary Fig. 5) suggested a truncated allele in the reference genome of cv. Khalas relative to the wild-type oil palm protein. Phylogenetic analysis was conducted by downloading plant sequences identified as homologues to oil palm VIR48, aligning with MUSCLE69 and reconstructing the phylogeny from a 105 amino acid conserved region spanning the R2R3 domain with the Wheelan and Goldman substitution model (gamma parameter=4) in PhyML (version 3.0; Fig. 6b).
Annotation of TEs in the R2R3 myb-like gene region of the reference assembly with CENSOR (http://www.girinst.org/censor) identified a copia-like LTR retrotransposon that interrupts the homologous sequence in oil palm (Fig. 6b) in exon 3 of the date palm gene. The possibility of a reference genome assembly error in exon 3 of the copia-like insertion allele (VIRcopia) in cv. Khalas was excluded by PCR+Sanger sequencing of genomic DNA from two varieties with yellow fruits (cv. Horra and cv. Lulu), which both confirmed the junction between the copia-like element and the 5′ end of exon 3. Characterization of the C-terminal end of VIR was complicated by the absence of sequence homologous to the oil palm VIR downstream of the copia-like element due to an apparent mis-assembly of scaffold S000271 in the vicinity of the copia-like element. However, we identified a scaffold (S022998) in the reference assembly with sequence homologous to exon 3 of oil palm VIR. Using a primer in this region paired with primers at the 5′ end of the gene on scaffold S000271, we PCR amplified and Sanger sequenced the 5′ and 3′ breakpoints between the copia-like insertion and the exon 3 from cDNA of a yellow-fruited variety (cv. Lulu). Inspection of the Sanger-based sequencing traces revealed a simple insertion of the element into exon 3 (Fig. 6d). Comparison of cDNA and genomic DNA sequences from the 5′ UTR through the copia-like insertion in cv. Lulu confirmed the existence of a stop codon at position 169 of the VIRcopia allele and confirmed the intron–exon structure of NCBI Gene ID LOC103717680, but suggest a mis-annotation of the corresponding gene (KacstDP.gene.S000271.14) in the annotation used throughout this study12. Although we were unable to amplify across the entire copia-like element from genomic DNA, these observations suggest that the annotated terminal stop in cv. Khalas LOC103717680 (Supplementary Fig. 5) is introduced by a nonsense mutation in a copia-like element, which truncates the protein relative to wild-type VIR in oil palm48.
Inspection of the short-read alignments suggested the copia-like sequence in the cv. Khalas assembly was absent in cultivars that produce red fruit. To characterize wild-type (VIR+) in date palm, we initially used Velvet70 to de novo assemble unmapped reads from a red-fruited variety (cv. Ajwa) and confirmed the existence of a contig with an exon 3 sequence homologous to oil palm VIR. By using the primer downstream of the copia-like insertion in VIRcopia in a yellow-fruited cultivars (see above), we successfully PCR amplified and sequenced a complete wild-type allele from both genomic DNA and cDNA in a red-fruited variety (cv. Khenezi). This confirmed the existence of a complete open reading frame of VIR+ in date palm that is predicted to encode a protein 231 amino acids in length. The intron–exon structure of VIR+ includes two introns and three exons including a complete exon 3, which is the same as VIRcopia with the exception of the copia-like insertion in exon 3. An intact third exon was subsequently confirmed by PCR+Sanger sequencing of genomic DNA from a second cv. with red fruit (cv. Um al Blaliz). Reverse transcription PCR of cDNA from each VIRcopia and VIR+ homozygotes (cv. Lulu and cv. Khenezi) confirm that both alleles are expressed at 105 days post-pollination in khalal stage fruit (Supplementary Fig. 6).
Genotypes at the VIR locus (Supplementary Table 10) were determined through manual inspection of the short-read alignments. A 2 × 3 contingency chi-square test was conducted to test the null hypothesis of independence between genotype and khalal stage fruit colour (Fig. 6e).
SNP and deletion validation
Nineteen SNPs predicted by the SNP-calling and filtering approach above were randomly chosen for validation by PCR+Sanger sequencing (Supplementary Table 3). Primers were designed flanking the focal SNP and one sample predicted to be homozygous for the reference allele and one sample predicted to be homozygous for the alternate was sequenced. Gene deletions relative to the reference assembly were validated using a PCR-based approach. Primers were designed in genes predicted to be deleted and amplifications attempted in a sample predicted to have the insertion allele and a sample predicted to be homozygous for the deletion. PCR products were then visualized using agarose gel electrophoresis (Supplementary Fig. 7).
Accession codes: The sequence data have been deposited into NCBI Sequence Read Archive (SRA) under project number PRJNA296800. Sequences for Sanger-based sequencing of the VIR gene have been deposited in the GenBank database under accession numbers KT734804 and KT734805.
How to cite this article: Hazzouri, K. M. et al. Whole genome re-sequencing of date palms yields insights into diversification of a fruit tree crop. Nat. Commun. 6:8824 doi: 10.1038/ncomms9824 (2015).
Meyer, R. S. & Purugganan, M. D. Evolution of crop species: genetics of domestication and diversification. Nat. Rev. Genet. 14, 840–852 (2013).
Purugganan, M. D. & Fuller, D. The nature of selection during plant domestication. Nature 457, 843–848 (2009).
Larson, G. et al. Current perspectives and the future of domestication studies. Proc. Natl Acad. Sci. USA 111, 6139–6146 (2014).
Xu, X. et al. Resequencing 50 accessions of cultivated and wild rice yields markers for identifying agronomically important genes. Nat. Biotechnol. 30, 105–111 (2012).
Wang, M. et al. The genome sequence of African rice (Oryza glaberrima) and evidence for independent domestication. Nat. Genet. 46, 982–988 (2014).
Miller, A. J. & Gross, B. From forest to field: perennial fruit crop domestication. Am. J. Bot. 98, 1389–1414 (2011).
McClure, K. et al. Genomics: a potential panacea for the perennial problem. Am. J. Bot. 101, 1780–1790 (2014).
Popenoe, P. The Date Palm Field Research Projects (1973).
Chao, C. T. & Krueger, R. R. The date palm (Phoenix dactylifera L.): overview of biology, uses, and cultivation. HortScience 42, 1077–1082 (2007).
Johnson, D. V. in: Date Palm Biotechnology (eds Jain S. M., Al-Khayri J. M., Johnson D. V. 1–11Springer: Netherlands, (2011).
Al-Dous, E. K. et al. De novo genome sequencing and comparative genomics of date palm (Phoenix dactylifera). Nat. Biotechnol. 29, 521–527 (2011).
Al-Mssallem, I. S. et al. Genome sequence of the date palm Phoenix dactylifera L. Nat. Commun. 4, 2274, doi:10.1038/ncomms3274 (2013).
Barrow, S. A revision of Phoenix L. (Palmae: Coryphoideae). Kew Bull. 53, 513–575 (1998).
Pintaud, J. C. et al. in: Diversity, Phylogeny, and Evolution in the Monocotyledons (eds. Seberg O., Petersen G., Barfod A. S., Davis J. I. 267–285Aarhus University Press (2010).
Tengberg, M. Beginnings and early history of date palm garden cultivation in the Middle East. J. Arid Environ. 86, 139–147 (2012).
Boivin, N. & Fuller, D. Shell middens, ships and seeds: exploring coastal subsistence, maritime trade and the dispersal of domesticates in and around the ancient Arabian Peninsula. J. World Prehist. 22, 113–180 (2009).
Beech, M. Archaeobotanical evidence for early date consumption in the Arabian Gulf. in: The Date Palm: From Traditional Resource to Green Wealth The Emirates Center for Strategic Studies and Research (2003).
Pelling, R. Garamantean agriculture and its significance in a wider North Africa context: the evidence of plant remains from the Fazzan Project. J. North Afr. Stud. 10, 397–411 (2005).
Nixon, S., Murray, M. A. & Fuller, D. Q. Plant use at an early Islamic merchant town in the West African Sahel: the archaeobotany of Essouk-Tadmakka (Mali). Veg. Hist. Archaeobot. 20, 223–239 (2011).
Elhoumaizi, M. A., Devanand, P. S., Fang, J. G. & Chao, C. C. T. Confirmation of ‘Medjool’ date as a landrace variety through genetic analysis of ‘Medjool’ accessions in Morocco. J. Am. Soc. Hortic. Sci. 131, 403–407 (2006).
DePristo, M. A. et al. A framework for variation discovery and genotyping using next-generation DNA sequencing data. Nat. Genet. 43, 491–498 (2011).
McKenna, A. et al. The Genome Analysis Toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data. Genome Res. 20, 1297–1303 (2010).
Li, H. Towards better understanding of artifacts in variant calling from high-coverage samples. Bioinformatics 20, 2843–2851 (2014).
Flutre, T., Duprat, E., Feuillet, C. & Quesneville, H. Considering transposable element diversification in de novo annotation approaches. PLoS ONE 6, e16526 (2011).
Kawuki, R. S., Ferguson, M., Labuschagne, M., Herselman, L. & Kim, D. J. Identification, characterisation and application of single nucleotide polymorphisms for diversity assessment in cassava (Manihot esculenta Crantz). Mol. Breeding 23, 669–684 (2009).
International Peach Genome Initiative. The high-quality draft genome of peach (Prunus persica) identifies unique patterns of genetic diversity, domestication and genome evolution. Nat. Genet. 45, 487–494 (2013).
This, P., Martinez-Zapater, J., Peros, J. P. & Lacombe, T. Natural variation in Vitis. edited by Francoise A., Blondon A., Martinez-Zapater J. In Genetics, Genomics, and Breeding of Grapes CRC Press (2011).
Hamblin, M. T., Buckler, E. S. & Jannink, J.-L. Population genetics of genomics-based crop improvement methods. Trends Genet. 27, 98–106 (2011).
Pintaud, J. C. et al. Biogeography of the date palm (Phoenix dactylifera L., Arecaceae): insights on the origin and on the structure of modern diversity. Acta Hortic. 994, 19–38 (2013).
Mathew, L. S. et al. A genome-wide survey of date palm cultivars supports two major subpopulations in Phoenix dactylifera. G3 5, 1429–1438 (2015).
Zheng, X. et al. A high-performance computing toolset for relatedness and principal component analysis of SNP data. Bioinformatics 28, 3326–3328 (2012).
Saitou, N. & Nei, M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425 (1987).
Pritchard, J. K., Stephens, M. & Donnelly, P. Inference of population structure using multilocus genotype data. Genetics 155, 945–959 (2000).
Evanno, G., Regnaut, S. & Goudet, J. Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol. Ecol. 14, 2611–2620 (2005).
Haubold, B., Pfaffelhuber, P. & Lynch, M. mlRho—a program for estimating the population mutation and recombination rates from shotgun-sequenced diploid genomes. Mol. Ecol. 19, 277–284 (2010).
Kaplan, N. L., Hudson, R. & Langley, C. H. The ‘hitchhiking effect’ revisited. Genetics 123, 887–899 (1989).
Nielsen, R. Molecular signatures of natural selection. Annu. Rev. Genet. 39, 197–218 (2005).
Kim, Y. & Stephan, W. Detecting a local signature of genetic hitchhiking along a recombining chromosome. Genetics. 160, 765–777 (2002).
Jimenez-Bermudez, S. et al. Manipulation of strawberry fruit softening by antisense expression of a pectate lyase gene. Plant Physiol. 128, 751–759 (2002).
Jain S. M., Al-Khayri J. M., Johnson D. V. (eds) Date Palm Biotechnology Springer (2011).
Cao, J. et al. Whole-genome sequencing of multiple Arabidopsis thaliana populations. Nat. Genet. 43, 956–963 (2011).
El Hassni, M. et al. Biological control of bayoud disease in date palm: selection of microorganisms inhibiting the causal agent and inducing defense reactions. Environ. Exp. Bot. 59, 224–234 (2007).
McHale, L., Tan, X., Koehl, P. & Michelmore, R. W. Plant NBS-LRR proteins: adaptable guards. Genome Biol. 7, 212 (2006).
Shiu, S. H. & Bleecker, A. B. Plant receptor-like kinase gene family: diversity, function, and signaling. Sci. STKE 2001, re22 (2001).
Kruijt, M., DE Kock, M. J. & de Wit, P. J. Receptor-like proteins involved in plant disease resistance. Mol. Plant Pathol. 6, 85–97 (2005).
Sedra, MyH. in: Date Palm Biotechnology (eds Jain S. M., Al-Khayri J. M., Johnson D. V. 533–550Springer (2011).
Tian, D., Araki, H., Stahl, E., Bergelson, J. & Kreitman, M. Signature of balancing selection in Arabidopsis. Proc. Natl Acad. Sci. USA 99, 11525–11530 (2002).
Singh, R. et al. The oil palm VIRESCENS gene controls fruit colour and encodes an R2R3-MYB. Nat. Commun. 5, 4106 (2014).
Hildebrandt, F. et al. A systematic approach to mapping recessive disease genes in individuals from outbred populations. PLoS Genet. 5, e1000353 (2009).
Lander, E. S. & Botstein, D. Homozygosity mapping—a way to map human recessive traits with the DNA of inbred children. Science 236, 1567–1570 (1987).
Li, H. & Durbin, R. Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics 25, 1754–1760 (2009).
Yang, M. et al. The complete chloroplast genome sequence of date palm (Phoenix dactylifera L.). PLoS ONE 5, e12762 (2010).
Li, H. Improving SNP discovery by base alignment quality. Bioinformatics 27, 1157–1158 (2011).
Cingolani, P. et al. A program for annotating and predicting the effects of single nucleotide polymorphisms, SnpEff: SNPs in the genome of Drosophila melanogaster strain w1118; iso-2; iso-3. Fly 6, 80–92 (2012).
Skinner, M. E., Uzilov, A. V., Stein, L. D., Mungall, C. J. & Holmes, I. H. JBrowse: a next-generation genome browser. Genome Res. 19, 1630–1638 (2009).
Bourgis, F. et al. Comparative transcriptome and metabolite analysis of oil palm and date palm mesocarp that differ dramatically in carbon partitioning. Proc. Natl Acad. Sci. USA 108, 12527–12532 (2011).
Trapnell, C., Pachter, L. & Salzberg, S. TopHat: discovering splice junctions with RNA-Seq. Bioinformatics 25, 1105–1111 (2009).
Jakobsson, M. & Rosenberg, N. A. CLUMPP: a cluster matching and permutation program for dealing with label switching and multimodality in analysis of population structure. Bioinformatics 23, 1801–1806 (2007).
Earl, D. A. & Vonholdt, B. M. STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conserv. Genet. Resour. 4, 359–361 (2012).
Gronau, I., Hubisz, M. J., Gulko, B., Danko, C. G. & Siepel, A. Bayesian inference of ancient human demography from individual genome sequences. Nat. Genet. 43, 1031–1034 (2011).
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Mol. Biol. Evol. 30, 2725–2729 (2013).
Nielsen, R., Korneliussen, T., Albrechtsen, A., Li, Y. R. & Wang, J. SNP calling, genotype calling, and sample allele frequency estimation from new-generation sequencing data. PLoS ONE 7, e37558 (2012).
Vieira, F. G., Fumagalli, M., Albrechtsen, A. & Nielsen, R. Estimating inbreeding coefficients from NGS data: impact on genotype calling and allele frequency estimation. Genome Res. 23, 1852–1861 (2013).
Danecek, P. et al. The variant call format and VCFtools. Bioinformatics 27, 2156–2158 (2011).
Koski, L. B., Gray, M. W., Lang, B. F. & Burger, G. AutoFACT: an automatic functional annotation and classification tool. BMC Bioinformatics 6, 151 (2005).
Lechner, M. et al. Proteinortho: detection of (co-)orthologs in large-scale analysis. BMC Bioinformatics 12, 124 (2011).
Sanseverino, W. et al. PRGdb 2.0: towards a community-based database model for the analysis of R-genes in plants. Nucleic Acids Res. 41, D1167–D1171 (2013).
Flowers, J. M., Hanzawa, Y., Hall, M. C., Moore, R. C. & Purugganan, M. D. Population genomics of the Arabidopsis thaliana flowering time gene network. Mol. Biol. Evol. 26, 2475–2486 (2009).
Edgar, R. C. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32, 1792–1797 (2004).
Zerbino, D. R & Birney, E. Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res. 18, 821–829 (2008).
We thank Marc Arnoux and Brianna Haining for technical assistance in the laboratory, Walter Sanseverino for providing R gene predictions, Sarah Seifi for helping compile the R genes, Florian Maumus for providing REPET predictions of repeats in the date palm genome, Robert J. Williamson for providing the script for heterozgosity calculations, Thomas M. Cahill for the fruit photos, Katherine Dorph for compiling fruit data and Dorian Fuller for illuminating discussions. We also thank Benoit Marchand and Muataz Barwani at New York University Abu Dhabi for assistance with high performance computing, and Nizar Drou for setting up JBrowse. This research was carried out on the High Performance Computing resources at New York University Abu Dhabi. This work was funded in part by grants from the NYU Abu Dhabi Research Institute and the US National Science Foundation Plant Genome Research Program (NSF PGRP), as well as a Human Frontier Science Program Postdoctoral Fellowship to U.R. and an NSF PGRP Postdoctoral Fellowship to R.S.M. J.A.M.'s contribution to this work was supported by a grant from Qatar Foundation National Research Priorities, NPRPX-014-4-001.
The authors declare no competing financial interests.
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Hazzouri, K., Flowers, J., Visser, H. et al. Whole genome re-sequencing of date palms yields insights into diversification of a fruit tree crop. Nat Commun 6, 8824 (2015). https://doi.org/10.1038/ncomms9824
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