The field of molecular electronics aims at using single molecules as functional building blocks for electronics components, such as switches, rectifiers or transistors. A key challenge is to perform measurements with atomistic control over the alignment of the molecule and its contacting electrodes. Here we use atomic force microscopy to examine charge transfer between weakly coupled pentacene molecules on insulating films with single-electron sensitivity and control over the atomistic details. We show that, in addition to the imaging capability, the probe tip can be used to control the charge state of individual molecules and to detect charge transfers to/from the tip, as well as between individual molecules. Our approach represents a novel route for molecular charge transfer studies with a host of opportunities, especially in combination with single atom/molecule manipulation and nanopatterning techniques.
The functionality of molecules1,2,3,4 for use as electronics components5 is usually probed by conductance measurements, with the molecule sandwiched between metal electrodes6,7. Because the metal/single molecule/metal junction is sensitive to every microscopic detail, control over the atomistic configuration of the junction is essential for obtaining meaningful results. Using the tip of a scanning tunnelling microscope (STM), this degree of control could be achieved in a few cases8. In general, however, the atomistic details are unknown, and conductance histograms of thousands of measurements are statistically analysed to find the most probable configurations of the molecular junction. Today, a well-established scheme for measuring conductance as a function of gap separation and voltage is the break junction technique9,10,11, in which a molecular junction is repeatedly formed/broken, and a single molecule is located between the electrodes.
We circumvent the issue of dealing with stochastically defined junctions by investigating molecular charge transport in a planar geometry (Fig. 1). In our approach, single molecules and molecular assemblies are arranged on a bulk-like insulating film, and the tip of an atomic force microscope (AFM) is used to control and detect their charge state. Owing to the high sensitivity of the AFM, intermolecular charge transfer can be measured with single-electron sensitivity12,13,14, and charge state detection is also possible at large tip-molecule separations. While with our approach we demonstrate molecular charge transfer only in the weak-coupling regime, it does not preclude the investigation of molecular assemblies including also molecules more strongly coupled to electrodes. The combination of our method with atom/molecule manipulation15 and top-down nanopatterning techniques might open up the prospect of studying atomically defined molecular junctions in the future.
Charge state control by AFM
Pentacene molecules adsorbed on 13 monolayer (ML) and as well as >20-ML thick NaCl films were selected as a model case, because well-ordered, defect-free NaCl films with large average terrace widths can be grown using standard epitaxy methods in ultra-high vacuum. At the larger film thicknesses (>20 ML )used, charge transfer to/from the substrate via the film is suppressed, and transport of single-electron charges is only possible between molecules as well as to/from the tip. At sufficiently large tip-molecule separations, charge exchange with the tip is also suppressed, and, hence, charges are conserved on the NaCl surface. Pentacene adsorbed on bilayer NaCl films has been intensively studied by STM16,17 and AFM18, and is readily identified via high-resolution AFM images obtained with pentacene-decorated tips18.
By positioning the AFM tip above a pentacene molecule and ramping the sample bias, we could reversibly switch the pentacene's charge state between negative, neutral and positive by attaching/detaching single electrons to/from the lowest unoccupied molecular orbital and highest occupied molecular orbital levels via the probe tip19, as schematically shown in Fig. 2a. Manipulation of the charge state entails a change of the local contact potential difference. Kelvin probe parabolas (dashed curves) corresponding to different charge states are shifted both horizontally and vertically. The direction of the horizontal shift of the parabola is a direct measure of the change of the local contact potential difference and enables discrimination of charge states20. The amount of the vertical and horizontal shift of the Kelvin probe parabolas depends also on the tip properties, that is, depending on the tip, we observe, for example, the vertical jump upon charging the lowest unoccupied molecular orbital to be also in the upward direction. In the experiments shown herein, charge state detection was also possible by the distinct frequency shifts at zero bias [Δf(V=0 V)], which allowed us to acquire charge-distribution maps by recording constant-height Δf-images at large tip-sample separations (z) with vanishing tunnelling probability across the vacuum gap. As an example Fig. 2g–j shows the sequential charging of four pentacenes spaced more than 50 Å apart including positive and negative charge states.
Figure 2b depicts an experimental Δf(V) charging curve for the positive charging of a pentacene molecule by detachment of an electron from the highest occupied molecular orbital level. The electron’s passage from the molecule to the tip appears as a jump-like feature in the Δf(V) signal19. The reverse process, formation of the neutral pentacene, is shown in Fig. 2c. The jumps in Δf(V) are observed at significantly different voltages in Fig. 2b,c which we attribute to the relaxation of the molecule-substrate system. We would like to point out that the observed voltage difference between the jump positions is not a direct measure of the relaxation energy, but varies strongly with the tip-pentacene distance and the bias ramp velocity. Moreover, the observed jump positions fluctuated statistically within a bias range of ±0.15 V, which depends also strongly on the tip-pentacene distance and the bias ramp velocity. Furthermore, for certain tip terminations, we detect a systematic shift of the jump voltages by more than 0.2 V, which we assign to different tip work functions20. Analogous charge state manipulation spectra for the formation and neutralization of a pentacene anion are shown in Fig. 2d,e, respectively.
Coulomb repulsion between charged molecules
For assemblies of pentacene molecules, as shown in Fig. 3a–c, we could observe the effect of Coulomb interaction during charging. For a single pentacene molecule (Fig. 3d), the Δf(V)-charging curve reveals detachment of an electron from the highest occupied molecular orbital orbital at V=−2.8 V (ref. 16). In the case of two adjacent pentacene molecules (8 Å centre to centre distance), as shown in Fig. 3e, the bias voltage for detaching the first electron remains the same, but the bias voltage has to be about 0.6 V more negative to detach a second electron. This value includes a voltage drop across the 13 ML NaCl film which we estimate to be at least 35% of the applied bias21,22. For comparison we calculated the corresponding Coulomb energy using a simple point charge model with charges located 2 Å above the NaCl surface and including the image charges in the NaCl to be 0.32 eV. The observation of Coulomb interaction can also reveal some useful information about the localization of charges within a molecular assembly. For example, for the three adjacent pentacene molecules shown in Fig. 3f, the measured Coulomb repulsion allowed us to infer the charge configuration. We attribute the first change in Δf to charging of the middle or one of the outer pentacene molecules as the first step. From basic electrostatic considerations we deduce that the additional small decrease (compared with Fig. 3e) of the bias voltage necessary for detaching the second electron is only consistent with the two positive charges being on the outer pentacene molecules. This assignment is further confirmed by the larger voltage shift of 0.7–0.8 V for detaching the third electron. As this value is significantly smaller than the value of 1.2 V, that is, two times the charging value observed for the previous case of two pentacenes, it might be that screening by the neighbouring pentacenes has to be taken into account and is subject of further investigations. We would like to point out that for some of the transitions, within a small energy window, apparently repeated transfer of electrons between the tip and molecule is observed and we attribute this to a close tip-sample distance, that is, a small tunnelling barrier, in Fig. 3. This effect is not observed at larger distance. The small artifacts at higher voltages cannot be explained at the moment. The charging curves of the assembly of three pentacene molecules for three different tip positions is shown in Supplementary Fig. 2.
Lateral charge transfer between individual molecules
By investigating a pair of pentacenes with a larger spacing, 18 Å centre to centre distance, and therefore a much lower intermolecular tunnelling rate compared with the case of Fig. 3 we can directly observe lateral charge transfer of single electrons (Fig. 4). These experiments were performed on a film with a thickness higher than 20 ML. While we could not experimentally determine the exact thickness of the film, we experimentally confirmed that charge transfer to the substrate was suppressed for more than 24 h.
The lateral charge transfer between the two pentacene molecules is exemplary shown by the Δf(V) trace in Fig. 4a. Each of the four dotted Kelvin probe parabolas in this graph corresponds to a different charge configuration (00, +0, 0+ and ++) and can be assigned with a few simple considerations: (i) from the measurements on a single molecule we can deduce that the Kelvin probe parabolas shift upwards and further to the left upon each electron detachment (Fig. 2a) and (ii) the absolute value of the frequency shift sensed by the tip monotonically decreases with increasing distance between the charged object and the tip. By means of (i), the outermost Δf traces are directly identified as (00) and (++). Because of the position of the tip throughout the measurement, on top of the left molecule (see the inset of Fig. 4a), a change of the charge state of the left molecule (right molecule) in the pair will have the biggest (lowest) impact on Δf. Thereby the Δf traces corresponding to (+0) and (0+) are identified. To further confirm this assignment of the four charge configurations we have also directly imaged them using constant-height mode AFM, as shown in Fig. 4b. These images are obtained by ramping the bias voltage and performing the same Δf(V) as in Fig. 4a, but manually stopping the ramp whenever a particular parabola has been reached. Such an experiment is possible because the average residence time in each parabola is at least on the order of a few seconds. Doing so enables us to perform constant-height Δf maps, similar as in Fig. 2g–j. The imaging procedure consists of retracting the tip by 20 Å from the Δf set point of 0.5 Hz at 0 V and imaging in the detection region of Fig. 1. Each image in Fig. 4b takes ∼20 min but provided that the tip is sharp enough yields enough resolution as to clearly determine where an electron has been detached from the pentacene pair.
With the charge states assigned, we can directly follow the lateral charge transfer by analysing the time dependence of Δf shifts. First the pair is in the (00) state. At about −3 V the charge configuration switches to the (+0) state by detaching an electron from the left molecule to the tip. Further ramping the voltage to more negative values, we observe a shift to the (0+) state near −3.3 V. As we can rule out transfer of charge to the substrate, this evidences that the charge transfer from one molecule to the other has occurred. Finally at slightly more negative sample voltage another electron is detached from the left molecule and both molecules are charged now positively (++).
Apart from the simple two-molecule case described above, we have also studied an assembly of three molecules, resulting in a total of eight charge configurations, of which two are degenerate. Correspondingly six different Kelvin probe parabolas can be clearly identified, as shown in Supplementary Fig. 3, but as loss of charge to the substrate could unfortunately not always be ruled out, the interpretation of the data with regard to lateral charge transfer is not as well defined as in Fig. 4a.
We find that the tunnelling of charges to the substrate is suppressed on thicker films (Fig. 4). The fact that on these bulk-like NaCl films charges are stabilized is an essential prerequisite to study lateral charge transfer and more universal compared with energetic charge state stabilization of adsorbates using suitable substrate systems23,24. The observed transfer rate is determined by the tunnelling barrier between the molecules and the relaxation energy, which significantly shifts the energy levels of the orbitals and strongly reduces the transfer rate in this case25,26. We attribute the dominant part of the relaxation energy to the interaction with the ionic substrate. While we cannot provide an experimental value for the relaxation energy in the case of pentacene on NaCl, we would like to point out that on the same substrate but for Cl surface vacancies a relaxation energy close to 1 eV was calculated and a phonon mediated level broadening of 350 meV was measured26. This would result in a very strong decrease of the transfer rate due to the relaxation energy. Furthermore, due to the relaxation energy the transfer rate depends critically on the electric field of the tunnelling tip, as it shifts the energy of a molecular state directly below the tip more than the ones of neighbouring molecules. To demonstrate the importance of this local level shift we have also studied the transfer rate without influence of the tip. Under such conditions the charge transfer rate decreased by many orders of magnitude as compared to the situation in the presence of the field of the tip, as for example shown in (Fig. 4) (Tip-sample distance 12 Å, film thickness about 30 ML). This very small transfer rate for already closely spaced molecules made a reliable measurement of the distance dependence of the transfer rate without field not feasible in the present case.
In summary, we have shown that by placing the AFM tip in suitable positions above a molecular assembly, we are able to distinguish and control different charge configurations and follow the development of the charge states in time, identifying lateral charge transfer between the molecules, or between the molecules and substrate/tip. We expect that further experiments and more detailed modelling will allow us to get more quantitative information on the parameters controlling the lateral charge transport on an ionic substrate, in particular on the relaxation energy, the tunnelling barrier height and width, and the lateral distribution of the electrical field from the tip.
Multilayer NaCl/Cu(111) films were prepared by evaporation of NaCl from a molecular beam evaporator onto a Cu(111) single crystal at room temperature. The NaCl/Cu(111) films were post annealed at 500 K for 5 min. Thirteen to twenty ML and thicker NaCl films were prepared. The Cu(111) single crystal was cleaned by repeated cycles of Ne+ sputtering and short annealing periods at 900 K before film preparation. Pentacene molecules were deposited at 5 K by vacuum sublimation from a direct current heated Si wafer. An overview image of the pentacene molecules on the 13 ML NaCl film is shown in Supplementary Fig. 4.
Charge state manipulation by AFM
AFM experiments were performed at 5 K in a custom-built low-temperature STM/AFM based on the qPlus sensor design27, in which the vertical oscillation of a quartz cantilever is measured piezoelectrically (resonance frequency f0=30.1 kHz, spring constant k=1,800 Nm−1, quality factor Q=13,700). For charge state manipulation, the tip was first positioned at a distance ≈15 Å away from the surface. At this distance, the tunnelling probability through the gap vanishes, and charge states are inherently stabilized because tunnelling through the film is also suppressed19. Next, the tip was positioned above a pentacene molecule, and the tip-pentacene distance and the sample bias were varied following the sequence A→B→C→D for charging, or B→A→D→C for decharging, depending on the desired charge state manipulation. In step A, the tip was approached to the surface by 6 Å to increase the tunnelling probability across the gap. In step B, the sample bias was ramped from 0 V to a certain voltage (depending on the desired charge state). In step C, the tip was retracted by 6 Å from the surface to suppress charge transfer across the gap. In step D, the sample bias was ramped back to 0 V. All images have been taken at 0 V sample bias.
How to cite this article: Steurer, W. et al. Probe-based measurement of lateral single-electron transfer between individual molecules. Nat. Commun. 6:8353 doi: 10.1038/ncomms9353 (2015).
We thank R. Allenspach for helpful comments. Financial support by the EU project ‘ARTIST’ (Contract No. 243421), ‘PAMS’ (Contract. No. 610446), EU project ‘ACRITAS’ (Contract No. 317348) and the ERC Advanced Grant ‘CEMAS’ is gratefully acknowledged.
Supplementary Figures 1-4