Better antibiotics capable of killing multi-drug-resistant Mycobacterium tuberculosis are urgently needed. Despite extensive drug discovery efforts, only a few promising candidates are on the horizon and alternative screening protocols are required. Here, by testing a panel of FDA-approved drugs in a host cell-based assay, we show that the blockbuster drug lansoprazole (Prevacid), a gastric proton-pump inhibitor, has intracellular activity against M. tuberculosis. Ex vivo pharmacokinetics and target identification studies reveal that lansoprazole kills M. tuberculosis by targeting its cytochrome bc1 complex through intracellular sulfoxide reduction to lansoprazole sulfide. This novel class of cytochrome bc1 inhibitors is highly active against drug-resistant clinical isolates and spares the human H+K+-ATPase thus providing excellent opportunities for targeting the major pathogen M. tuberculosis. Our finding provides proof of concept for hit expansion by metabolic activation, a powerful tool for antibiotic screens.
The constant spread of drug-resistant pathogens requires the development of innovative drug-screening methods that generate a substantial amount of lead compounds1. The prototype of a difficult-to-treat pathogen with hampered control due to multi-drug resistance (MDR) is Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB). Despite greatly increased efforts to identify and develop new therapeutic agents, the TB pandemic continues to be a major cause of morbidity and mortality worldwide. In 2013, TB was responsible for an estimated 1.5 million deaths. In the same year, 480,000 people developed MDR-TB2. The outcome data for patients starting MDR-TB treatment in 2011 show a success rate of only 48%, due to lack of effective treatment regimens2. To combat this global health problem, the rapid development of safe drugs with new mechanisms of action is required.
In the past decade concerted screening of millions of small molecules for growth inhibition of Mtb led to the identification of a few promising compounds, some of which are now in clinical trials3. However, due to limitations in both the diversity of chemical libraries and the seemingly limited number of druggable targets, remarkably few truly novel hits were identified in these basic whole-cell screens4. Host cell-based phenotypic assays that better reflect the biology of the pathogen may overcome this bottleneck by avoiding the bias of synthetic media-driven target selection5,6. Another efficient approach to fight notoriously drug-resistant pathogens such as Mtb is repurposing of existing drugs, and their analogues, which reduces drug development costs and saves precious time7.
When testing Food and Drug Administration (FDA)-approved drugs in an innovative high-throughput screen selecting for compounds that abrogate Mtb-induced cytotoxicity8, we focused on non-antibiotic hits of clinically approved pharmacophores active against MDR-TB. This screen identified the gastric proton-pump inhibitor (PPI) lansoprazole (LPZ) as a compound with intracellular anti-mycobacterial activity. PPIs, selective inhibitors of the H+K+-ATPase of the gastric parietal cell, are used extensively for the treatment of acid-related disorders of the stomach9. As non-prescription drugs, PPIs have an excellent safety profile and are among the most widely sold drugs in the world10.
In this work, we show that LPZ is a prodrug that requires the host’s cytoplasm for conversion into a LPZ analogue with antituberculous activity. Successful target identification studies and the fact that the active compound fails to inhibit the human gastric H+K+-ATPase make this metabolite an attractive lead compound for the TB drug pipeline. Our findings establish host-cell-driven prodrug activation as an additional strategy for successful lead identification, which has a great potential of broadening the spectrum of existing small-molecule libraries.
LPZ inhibits growth of intracellular Mtb
We have recently generated a host cell-based high-throughput screen that selects for compounds that protect MRC-5 lung fibroblasts from Mtb-induced cytotoxicity8. In this assay, we infect fibroblasts with high multiplicities of infection (MOI of 10) in the presence of screening compounds (Supplementary Fig. 1). After 72 h of co-incubation, the majority of infected fibroblasts are killed by wild-type Mtb strains and killing can be quantified by fluorescent staining. Using this assay, we screened 1,280 FDA-approved drugs of the Prestwick chemical library at a concentration of 10 μM thus identifying the gastric PPI LPZ as a potent hit compound that protected fibroblasts at levels comparable to those of well-established anti-mycobacterial drugs (Fig. 1a; Supplementary Table 1).
When confirming LPZ as a hit in the primary screen, we also tested other widely used PPIs such as omeprazole and pantoprazole. Interestingly, these drugs failed to protect fibroblasts at concentrations up to 50 μM (Fig. 1a). This observation made a host-directed immunomodulatory effect leading to better intracellular clearance of Mtb, an unlikely mechanism since most PPIs show comparable activity on their eukaryotic target. However, testing 5-hydroxy-LPZ, which was equally inactive at 50 μM (Fig. 1a), suggested that substitutions on the benzimidazole ring may account for the inactivity of these close LPZ analogues (Supplementary Fig. 2).
Hit compound LPZ was tested for growth inhibition of intracellular Mtb cells expressing green fluorescent protein (GFP), at different drug concentrations. LPZ reduced the Mtb-GFP signal in a dose-dependent manner with a half-maximal inhibitory concentration (IC50) of 1.47 μM and this corresponded well with the protection of MRC-5 cells quantified in the same well (Fig. 1b). To rule out a cell-line-specific effect, we performed similar infection experiments using RAW264.7 macrophages in which growth of intracellular bacteria was inhibited with an IC50 of 2.2 μM (Fig. 1c,d). In contrast to these results, the anti-mycobacterial activity of LPZ in common mycobacterial broth was 22-fold higher (IC50 of 32.8 μM) than its activity in MRC-5 cells (Supplementary Fig. 3).
LPZ is converted to LPZS intracellularly
LPZ is a relatively unstable compound that can be modified in both enzymatic and non-enzymatic reactions11. Its activity on the human H+K+-ATPase requires the prodrug LPZ to be converted to a sulfenic acid or sulfenamide intermediate in the acidic environment of the stomach9. We reasoned that LPZ may be converted to an active antibiotic primarily in an intracellular environment, thereby explaining the discrepancy between the ex vivo and in vitro activity. Thus, we quantified intracellular LPZ and possible metabolites over a period of 48 h using liquid chromatography–electrospray ionization/mass spectrometry (LC-ESI/MS) and observed a rapid intracellular decay of LPZ and its near-quantitative conversion to a molecule of lower mass (m/z 354.0884, g mol−1) (Fig. 2a,b; Supplementary Table 2; Supplementary Fig. 4a). Using analogues as standards, we identified this molecule as lansoprazole sulfide (LPZS), a highly stable LPZ metabolite (Fig. 2c,d; Supplementary Fig. 4b)11. LPZS is a precursor for LPZ production that fails to form the sulfenic acid necessary for binding the gastric H+K+-ATPase9,12.
Rapid decay of LPZ was also observed in broth; however, LPZS was not the major product (Fig. 2e; Supplementary Table 2). Assuming that LPZS has anti-mycobacterial activity, this differential pattern of LPZ metabolism explains the better activity of the compound during intracellular infection. This hypothesis was confirmed by testing LPZS for growth inhibition of Mtb in broth and in intracellular assays. Strikingly, LPZS had a 71-fold improvement of activity compared with LPZ in broth (IC50 of 0.46 μM) (Fig. 2f) and showed similar intracellular activity (IC50 of 0.59 μM) (Fig. 2g). Thus, intracellular sulfoxide reduction converts LPZ to the potent anti-mycobacterial agent LPZS.
Having established LPZS as a compound with antibacterial activity, we were interested in its antibiotic spectrum. Intriguingly, LPZS showed a highly Mtb-selective antibiotic profile with good activity against drug-resistant isolates (Tables 1 and 2). Growth of several Gram-negative and Gram-positive bacteria was not affected by LPZS (Table 1). To determine the physiological significance of these findings, we tested the compound in the murine model of acute TB. Oral administration of LPZS significantly reduced the bacterial burden of Mtb-infected mice (Fig. 2h; in vivo pharmacokinetic data can be found in Supplementary Fig. 5). There were no signs of toxicity in mice treated with doses as high as 300 mg kg−1 b.i.d., owing to the favourable cytotoxicity profile of LPZS (Supplementary Table 3). We also performed in vitro drug combination studies with LPZS and several first- and second-line anti-TB drugs, where we observed additive effects for the tested combinations (Supplementary Table 4).
LPZS targets cytochrome b
To identify the target of LPZS, we raised drug-resistant mutants of Mtb and identified three that displayed stable phenotypic resistance (Fig. 3a). Whole-genome sequencing revealed a single-nucleotide polymorphism (SNP) that changed leucine-176 to proline in the b-subunit of the cytochrome bc1 complex (qcrB, Rv2196) in all three mutants (Fig. 3b). Reintroduction of this SNP into wild-type Mtb by recombineering confirmed causality (Fig. 3a, rMtb-L176P strain). As an essential respiratory chain component, cytochrome bc1 (complex III) is required for ATP production. Consistent with QcrB inhibition, we observed massive and rapid ATP depletion in treated Mtb (Fig. 3c). ATP levels of the L176P mutant strain were hardly affected by LPZS treatment (Supplementary Fig. 6a). To provide further proof for LPZS targeting cytochrome bc1, we measured ADP levels in LPZS-treated and untreated bacteria, which allowed us to calculate the ADP/ATP ratio. In untreated wild-type Mtb, this ratio was 3.5, LPZS treatment led to a 7-fold increase of intracellular ADP levels upon treatment and an ADP/ATP ratio of 25.8. Most importantly, this effect was not observed when the LPZS-resistant L176P mutant strain was tested (Supplementary Fig. 6b). Thus we provide strong evidence that QcrB is indeed the target protein of the LPZ metabolite LPZS, which is a highly unexpected finding since the gastric H+K+-ATPase and cytochrome bc1 are structurally unrelated protein complexes.
LPZS represents a novel class of QcrB inhibitors
QcrB is an emerging and highly vulnerable drug target of Mtb. Recently identified imidazopyridine amides (IPAs) display potent in vitro and in vivo activity against Mtb13,14. Many data for LPZS are similar to findings obtained with IPA compounds. Both classes share a highly Mtb-selective activity profile; they are bacteriostatic in broth and inactive against streptomycin-starved 18b (SS18b), a viable but conditionally non-replicating strain of Mtb (Supplementary Fig. 7a,b)15. Inactivity against SS18b is most likely due to upregulation of the cytochrome bd-oxidase that replaces QcrB, thus avoiding the effect of inhibitors such as LPZS16.
To visualize both the L176P mutation and the T313A mutation, which confers IPA resistance, we modelled the mycobacterial protein on the published QcrB structure of Rhodobacter sphaeroides17. Both mutations localized to the ubiquinol oxidation (Qp) site close to the Qp-inhibitor stigmatellin, which was co-crystalized with the R. sphaeroides protein (Fig. 4a). This indicates that both compounds target the same QcrB-active site; however, L176P mutants remained susceptible to several IPA compounds (Fig. 4b; Supplementary Table 5). Conversely, growth of the highly IPA-resistant T313A mutant was fully inhibited by LPZS, indicating distinct drug-binding mechanisms for the two drugs (Fig. 4b).
In this report, we present the combined use of host cell-based drug screens and ex vivo pharmacokinetics, which identified a novel class of antibiotic active against multi-drug-resistant Mtb. Our findings have several important implications: first of all and most intriguingly, LPZS is a highly attractive and safe antituberculous lead compound with in vivo activity that fails to inhibit the gastric H+K+-ATPase (Fig. 5). Future structure–activity relationship studies will clearly benefit from 40 years of intense research on PPIs and their analogues. Substantial amounts of in vitro and in vivo pharmacological data are available for PPI metabolites, thereby providing a rich source for repurposing these substituted benzimidazoles. Analogues with antituberculous activity will have predictable side effects and pharmacokinetic profiles, thereby accelerating translation into pre-clinical and clinical development.
Furthermore, we have confirmed QcrB as a highly specific and druggable Mtb target capable of accommodating chemically unrelated compounds with distinct binding mechanisms. The commercially available cytochrome b inhibitor LPZS provides a useful tool to further explore this multienzyme complex as a drug target and to study the mycobacterial respiratory chain that is not well understood despite its key role in adaptive processes during intracellular growth.
Several antimalarial drugs such as atovaquone bind the same pocket of plasmodial cytochrome b, which indicates that targeting the pathogen’s cytochrome bc1 complex is safe despite the presence of human mitochondrial orthologues18. Intriguingly, a recent report identified LPZ-like PPIs as potent inhibitors of plasmodial growth only upon infection of metabolically active liver cells19. The authors discuss metabolic transformation of the compounds as a possible cause for their observation, although the underlying mechanism or a drug target was not identified19. It is very likely that intracellular sulfoxide reduction, as described in our report, leads to antiplasmodial activity. Thus our findings can improve the future control of both malaria and TB, two leading causes of death.
The prodrug LPZ represents an excellent example of a valuable hit compound in an existing library that was missed by conventional drug screens. Using an innovative screen, we found a new activity for an old drug that supports the notion that novel screening platforms may uncover new antibiotics in old libraries1. We provide evidence that intracellular assays are capable of broadening the spectrum of existing small-molecule libraries by identifying drugs that require the host cell environment for their conversion to a bioactive metabolite. Up to 10% of drugs approved worldwide can be classified as prodrugs that are transformed by eukaryotic enzymes, such as esterases and phosphatases, or by non-enzymatic reactions driven by biochemical properties of the intracellular environment20. Using host cells to transform approved drugs into derivatives with antibacterial activity may render the drug inactive against its original human target, as is the case for LPZ and LPZS (Fig. 5)9. A substantial number of prodrugs can also be expected in small-molecule libraries containing compounds of unknown function. These molecules provide an untapped pool for antimicrobial drug discovery. Our strategy of host-mediated prodrug activation is generally applicable to other drug-resistant bacterial pathogens, thereby enabling inhibitors with novel targets to be found in compound libraries that have been interrogated previously in standard phenotypic screens.
Drugs used in this study
LPZ, omeprazole and pantoprazole were purchased from Sigma-Aldrich, LPZS and other LPZ analogues were purchased from Santa Cruz biotechnology, Toronto Research Chemicals Inc. and Alfa Aeser. Q203 was kindly provided by Kevin Pethe of the Institut Pasteur Korea. Other IPA compounds were derived from a GlaxoSmithKline library kindly provided by Lluis Ballell21.
Culture conditions of Mtb and eukaryotic cell lines
Mycobacterial strains were routinely grown in Middlebrook 7H9 broth (supplemented with 0.2% glycerol, 10% albumin dextrose catalase (ADC) and 0.05% Tween-80) or 7H10 agar plates (supplemented with 0.5% glycerol and 10% oleic ADC). MRC-5 human lung fibroblasts were provided by the Coriell Institute for Medical Research and grown in MEM supplemented with 10% heat-inactivated fetal bovine serum, 1% non-essential amino acids and 1 mM sodium pyruvate. RAW264.7 macrophages derived from the EPFL strain collection were grown in RPMI medium supplemented with 10% fetal bovine serum. Both cell lines were grown at 37 °C with 5% CO2.
High-throughput drug screen and intracellular assays
Compounds of the Prestwick chemical library were preplated into 384-well microplates (Corning) at a concentration of 100 μM in 5 μl of 5% dimethyl sulfoxide. MRC-5 cells grown to late-log phase were harvested and seeded at 4,000 cells per well in a volume of 35 μl using an automated microplate dispenser (multidrop combi, Thermo Scientific). Cells were allowed to adhere for 3 h and then were infected with washed logarithmic-phase Mtb Erdman cells at an MOI of 10 in 10 μl of MEM medium. Plates were sealed and incubated at 37 °C in 5% CO2. After 72 h, the plates were left at room temperature for 1 h and 5 μl of Prestoblue cell viability reagent (Life Technologies) were added. After 1 h at room temperature, fluorescence was measured in a Tecan Infinite M200 plate reader (excitation 570 nm and emission 590 nm). Mtb in infected macrophages or fibroblasts was quantified using an Mtb Erdman strain expressing EGFP and a Tecan plate reader (excitation 480 nm and emission 510 nm).
For fluorescence microscopy, RAW264.7 macrophages were seeded on round 9-mm coverslips in 24-well plates (105 cells per well). To quantify intracellular Mtb, macrophages were infected at an MOI of 2 for 12 h. Cells were washed several times to remove unphagocytosed bacteria and fresh medium containing compounds or dimethyl sulfoxide was added. After incubation for 4 days, the cells were washed and fixed with 4% paraformaldehyde/PBS and stained with Dapi-Fluoromount-G (Southern Biotech). Images were acquired on a Zeiss LSM 700 using ZEN imaging software and Fiji processing software. Resazurin reduction microplate assays (REMAs) were performed in 7H9 broth using a starting OD600 of 0.0001, a 7-day (or 14 day) incubation period and a final volume of 10% resazurin (0.025% w/v). After incubation, fluorescence of the resazurin metabolite resorufin was measured (excitation at 570 nm and emission at 590 nm, gain 80) using a TECAN Infinite M200. For OD600-based MIC determination, bacterial OD600 was adjusted to 0.01 and bacteria were exposed to LPZS for 7 days followed by OD readings. For reading after 14 days of exposure, initial OD600 was adjusted to 0.0001. Drug testing against streptomycin-starved 18b was performed as described above for REMA assays using an SS18b culture maintained in 7H9 medium without streptomycin for 2 weeks and a final OD600 of 0.1 (ref. 15).
ATP-depletion assay and ADP quantification
Log-phase Mtb cultures were exposed to drugs for 24 h and mixed with BacTiter-Glo reagent (v/v 4:1) (Promega) followed by incubation in the dark for 10 min. Luminescence (relative light units, RLUs) was read in a TECAN Infinite M200. For ADP detection and calculation of the ADP/ATP ratio, we exposed log-phase Mtb (OD600 of 0.01) to LPZS for 24 h in a white 96-well plate. BacTiter-Glo reagent (v/v 4:1) was added and luminescence was determined after 2 min (RLUA). Plates were incubated in the dark for 15 min and read again, which provided background ATP signals prior to ADP measurements (RLUB). Five microlitre of an ADP-converting enzyme (Sigma Alrich, MAK135E) were added, followed by a third measurement after 1 min of incubation (RLUC). The ADP/ATP ratio was calculated using the following formula: ADP/ATP=(RLUC−RLUB)/RLUA.
Culture conditions and REMA assay of other microorganisms
Mycobacterium strains were grown in 7H9 broth (Difco) supplemented with Middlebrook ADC enrichment, 0.2% glycerol, 0.05% Tween-80. Bacillus subtilis, Candida albicans, Corynebacterium glutamicum, Escherichia coli, Micrococcus luteus, Pseudomonas putida, Salmonella typhimurium and Staphylococcus aureus were grown in Luria broth base (Sigma). C. diphtheriae, Enterococcus faecalis, Listeria monocytogenes and P. aeruginosa were grown in brain heart infusion broth (Difco). Twofold serial dilutions of each test compound were prepared in 96-well plates containing bacteria in a total volume of 100 μl and then incubated at 37 or 30 °C (as required) before addition of 10 μl of 0.025% resazurin. After incubation, fluorescence of the resazurin metabolite resorufin was determined as described above. Colony-forming unit (c.f.u.) counts and checkerboard assays for drug combination studies were performed using REMA assays and twofold serial dilutions of LPZS in combination with the drugs mentioned in Supplementary Table 5. The fractional inhibitory index (ΣFIC) was calculated as FIC (fractional inhibitory concentrations) of compound X+FIC of compound Y to evaluate interaction profiles. The FIC was calculated using the following formula: FIC (X+Y)=[MIC of compound X in combination with Y]/[MIC of X alone]. ΣFICs of ≤0.5 designate synergistic activity and values >0.5 additive activity22,23.
Isolation and characterization of LPZS-resistant mutants
LPZS-resistant Mtb H37Rv mutants were isolated by plating 109 c.f.u. on 7H10 agar containing 20 μM of LPZS. Genomic DNA was isolated as described recently24. Whole-genome sequencing was performed using Illumina technology with sequencing libraries prepared using the NEBnext Ultra DNA kit (New England Biolabs). The resulting genomic DNA fragment library was loaded on Illumina MiSeq Reagent Kit V3 cartridges and sequenced. All Illumina reads were right-trimmed to a length of 150 nucleotides to remove low-quality areas. Each sample was downsampled to 1.5-M read pairs to even the genome coverage between samples. Analysis was done with MIRA (version 4.0rc4) using the Mtb H37Rv genome (NC_000962.3) as reference. The resulting SNP table was parsed in Excel to identify informative SNPs.
Recombineering method for target confirmation
Mtb H37Rv carrying plasmid pJV53 was grown to mid-log phase in 7H9 broth containing 25 μg ml−1 kanamycin and exposed to 0.2% acetamide for 16 h. Competent cells were co-transformed with 100 ng of single-stranded oligos (lagging and leading strand) (5′-CTCACTGCCTGACGACCTGCTGTCGGGACTCGGTCCGCGCGCGGCACTCTCGTCGATCACGCTGGGTATGC-3′—for the L176P mutation) or (5′-GGCGGGCTCGCAGCCAGACTTCTACATGATGTGGGCCGAGGGTCTGGCCCGGATCTGGCCGCCGTGGGAG-3′—for the T313A mutation) and pYUB412 (50 ng) followed by plating on 7H10 agar plates containing 50 μg ml−1 hygromycin. SNPs in hygromycin-resistant clones were confirmed by PCR and sequencing using oligos 5′-TGCTGATCACCGGCGTGTAT-3′ and 5′-AAGATGATCCCCGGCAACAG-3′ for L176P or 5′-TTCAAGTCCGGCGCATTTTT-3′ and 5′-TAGACGAACGGCGGCAGAAT-3′ for T313A.
Ex vivo pharmacokinetics and ESI–MS
For intracellular drug quantification, 20,000 MRC-5 cells were seeded in 96-well plates and exposed to 2 μM LPZ. At given time points, cells were extensively washed with PBS and lysed with 0.1% Triton X-100 in PBS and acetonitrile (1:1 ratio). After spinning at 15,000g at 4 °C, samples were shock-frozen in liquid nitrogen and stored at −80 °C for MS. Quantification in 7H9 broth was performed with 500 nM of LPZ, acetonitrile was added in a 1:1 ratio at given time points followed by spinning and shock freezing. The UPLC separation was done on an Agilent 1290 Infinity LC system including the 1290 Infinity LC system binary pump with an integrated degasser, the high-performance autosampler and a thermostatted column compartment. Samples (2 μl) were injected into a Zorbax Extend-C18 (2.1 × 50 mm, 1.8 μm) analytical column (Agilent Technologies) operated at 40 °C, using H2O–HCOOH 0.1% and CH3CN–HCOOH 0.1% as mobile phases A and B, respectively, (2–100% B in 5 min) at a flow rate of 0.4 ml min−1. All samples were injected in duplicate. The UPLC system was interfaced with a 6530 Accurate-Mass Q-TOF LC/MS system (Agilent Technologies). ESI–MS data were acquired in the positive ionization mode, in the mass range m/z 100–1,000 (2 spectra s−1). Experimental parameters were set as follows: fragmentor: 190 V, Vcap: 3,500 V, gas temperature: 300 °C and sheath gas temperature: 350 °C. External calibration was carried out with a solution of ESI_L (Agilent). Data were processed using MassHunter quantitative analysis compliance software. Extracted ion chromatogram of ions at m/z 354.088 and 370.083 were integrated and quantified using external calibration of both LPZ and LPZS spiked in the same matrix.
For pharmacokinetic studies, female BALB/c mice (18–20 g) were given 100 mg kg−1 or 300 mg kg−1 LPZS in 20% TPGS (D-α tocopheryl polyethylene glycol 1,000 succinate) by gavage. At given time points, blood from three mice was taken and, after spinning, serum was treated with acetonitrile (1:4 ratio). After a second spin, supernatants were frozen for LC/MS experiments. For in vivo efficacy studies, mice were treated following the acute infection protocol of Rullas et al.25 with some modifications. In brief, female BALB/c mice (18–20 g; 4 mice per group) were aerosol infected with Mtb H37Rv and treated the following day with 300 mg kg−1 LPZS b.i.d. by gavage for 9 days. The day after final treatment, mice were killed and serial dilutions of lung homogenates were plated on 7H10 agar containing 10 μg ml−1 cycloheximide and 25 μg ml−1 ampicillin. Experiments were approved by the Swiss Cantonal Veterinary Authority (authorization number 2218).
Student’s t-test was used throughout the paper to compare two groups.
Protein structure prediction
QcrB (Rv2196) from M. tuberculosis was modelled using the software iTasser ( http://zhanglab.ccmb.med.umich.edu/I-TASSER/) and the cytochrome bc1 structure from R. sphaeroides as template (PDB code 1QJY).
Accession codes: Genome sequencing data were deposited at the SRA database (NCBI) under the Study accession SRP049754.
How to cite this article: Rybniker, J. et al. Lansoprazole is an antituberculous prodrug targeting cytochrome bc1. Nat. Commun. 6:7659 doi: 10.1038/ncomms8659 (2015).
Sequence Read Archive
We are grateful to Laure Menin of the EPFL mass spectrometry facility for excellent support during MS experiments. We also thank Dr N. Dhar (Laboratory of Microbiology and Microsystems (LMIC), EPFL) for providing the Mtb Erdman-EGFP strain. M. Chambon, D. Banfi and N. Ballanfat (Biomolecular Screening Facility (BSF), EPFL) were of great help during assay development. This work was supported in part by grants from the Swiss National Science Foundation (grant number 31003A_140778). J.R. was supported by the German Federal Ministry of Research and Education (BMBF grant 01KI1017).
Supplementary Figures 1-7 and Supplementary Tables 1-5
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Archives of Pharmacal Research (2015)