Branched-chain aminotransferases (BCAT) are enzymes that initiate the catabolism of branched-chain amino acids (BCAA), such as leucine, thereby providing macromolecule precursors; however, the function of BCATs in macrophages is unknown. Here we show that BCAT1 is the predominant BCAT isoform in human primary macrophages. We identify ERG240 as a leucine analogue that blocks BCAT1 activity. Selective inhibition of BCAT1 activity results in decreased oxygen consumption and glycolysis. This decrease is associated with reduced IRG1 levels and itaconate synthesis, suggesting involvement of BCAA catabolism through the IRG1/itaconate axis within the tricarboxylic acid cycle in activated macrophages. ERG240 suppresses production of IRG1 and itaconate in mice and contributes to a less proinflammatory transcriptome signature. Oral administration of ERG240 reduces the severity of collagen-induced arthritis in mice and crescentic glomerulonephritis in rats, in part by decreasing macrophage infiltration. These results establish a regulatory role for BCAT1 in macrophage function with therapeutic implications for inflammatory conditions.
Branched-chain aminotransferase (BCAT) is the enzyme responsible for the reversible transamination of leucine, isoleucine and valine, the amino acids collectively known as branched-chain amino acids (BCAA). Transamination of BCAAs is the first step in their catabolism and results in the formation of branched-chain α-keto acids, which are decarboxylated to form derivatives of coenzyme A (CoA)1,2. In the case of leucine, transamination leads to the formation of α-ketoisocaproate, which is further metabolized to form the ketone body acetoacetate, and acetyl-CoA, which is subsequently oxidized in the tricarboxylic acid (TCA) cycle. This reaction equally results in the production of glutamate, which is another crucial metabolite that feeds into the TCA cycle at the level of α-ketoglutarate3. Hence, BCAT enzymes are multi-level regulators of the TCA cycle and oxidative phosphorylation, possibly contributing to metabolic reprogramming in eukaryotic cells.
BCAT exists in two isoforms, mitochondrial BCAT2 and cytosolic BCAT1. Although Bcat2 is expressed ubiquitously in most tissues and especially in skeletal muscle, tissues of the digestive system and the kidney, Bcat1 expression is reported to be limited to embryonic tissues, adult brain, ovary, placenta and neurons of the peripheral nervous system4. Despite the large body of work on BCAA metabolism and, in particular, Bcat1 activity in glutamate neurotransmitter metabolism in the brain4 and in tumour growth5, data on the role of BCAT1 and its potential metabolic effects on macrophages and inflammatory disease in general do not exist.
Macrophages are innate immune cells with a phenotype tightly linked to their metabolism6,7,8,9. They have remarkable plasticity in function, with both extreme and intermediate activation phenotypes10,11 under specific transcriptional control12. Toll-like receptor agonist lipopolysaccharide (LPS) stimulation results in a rapid and robust transcriptional response that involves genes that regulate metabolic reprogramming13. Macrophages activated by LPS undergo a metabolic shift from oxidative phosphorylation to glycolysis known as the Warburg effect9,14, with some important metabolic effectors regulating the balance between the two metabolic states15. The increased consumption of glucose is known to be associated with a proinflammatory macrophage phenotype16. Similar metabolic reprogramming has been detected in mouse dendritic cells, with an early response to LPS corresponding to an increase in glycolytic rate within minutes of exposure17. Macrophages activated by LPS also show accumulation of Krebs cycle intermediates, such as succinate, regulating Hif-1α-mediated IL-1β production14. A comprehensive integrative analysis of the transcriptome and metabolome in activated macrophages has established the importance of citrate-itaconic acid axis through Irg1 (also known as cis-aconitate decarboxylase, Acod1)18, one of the most significantly up-regulated transcripts in LPS-stimulated macrophages, which encodes an enzyme that catalyses the aconitate-to-itaconate reaction18,19. These studies led to the concept of the broken (or fragmented) Krebs cycle in LPS-activated macrophages (M(LPS))18,20, in which TCA cycle intermediate metabolites function as metabolic checkpoints for the activation of LPS response genes, such as Hif1a, Il1b, Irg1. Furthermore, these data show that reprogramming macrophage metabolism is not only needed for energy requirements, but also for the transcriptional regulation of the innate immune response.
By using high-throughput RNA measurements in human monocyte-derived macrophages, we show that BCAT1 is the most abundantly expressed BCAT isoform. Using a novel selective inhibitor of BCAT1 (ERG240, a leucine analogue), we show that inhibition of BCAT1 leads to the reduction of oxygen consumption and glycolysis together with a decrease in IRG1 and itaconate, but not HIF-1α and IL-1β messenger RNA (mRNA) and protein levels; suggesting induction of BCAT1-mediated anaplerosis through the leucine–citrate–itaconic acid axis in early macrophage activation with LPS. To confirm the effect of ERG240 in vivo, we show that peritoneal macrophages from mice injected with LPS and ERG240 have reduced levels of Irg1/itaconate together with a diminished proinflammatory transcriptome when compared with mice injected with LPS alone. RNAi of BCAT1 in human M (LPS) phenocopies the effect of pharmacological blockade with reduced BCAT1 protein levels resulting in reduction of oxygen consumption and glycolysis together with decreased IRG1 and itaconate levels. We then investigate the anti-inflammatory effects of ERG240 in two distinct murine models of autoimmune disease (rheumatoid arthritis and crescentic glomerulonephritis) characterized by macrophage activity and infiltration. We show that ERG240 treatment results in reduced inflammation associated with decreased macrophage infiltration in target organs (joints and kidney) in both models.
BCAT1 regulates metabolic reprogramming in macrophages
In a previous genome-wide expression quantitative trait loci analysis using inbred rat models of glomerulonephritis, we identified Bcat1 as the hub of a co-expression network in macrophages, suggesting its potential regulatory metabolic role in inflammatory disease21. To gain more insights into the role of BCAT enzymes in macrophages, we set out to investigate these in human monocyte-derived macrophages (hMDMs) by subjecting these cells to RNA-sequencing (Fig. 1a). This showed that mRNA levels of BCAT1 are markedly increased when compared to BCAT2, suggesting that the cytoplasmic isoform is the major source of BCAA catabolism in these cells. To examine the role of BCAT1 in macrophage metabolism and inflammatory diseases, we identified a novel BCAT1 inhibitor, ERG240, using molecular modelling approaches based on the X-ray crystal structures of BCAT1 (ref. 22). ERG240 is a water-soluble structural analogue of leucine (Fig. 1b). Its ability to suppress the BCAT1 aminotransferase activity was measured in a continuous fluorometric assay (ref. 23 and see Supplementary Methods). We found that ERG240 inhibited recombinant BCAT1 with an IC50 of 0.1–1 nM, while no inhibition was observed on BCAT2 (Fig. 1c). The effect of BCAT1 inhibition on hMDMs was studied in control (basal, M(basal)) or LPS-stimulated cells by measuring mRNA and protein levels of three major TCA-regulated early macrophage activation markers: HIF-1α, IL-1β (ref. 14) and IRG1 (ACOD1)18. As expected, LPS treatment caused the up-regulation of HIF-1α, IL-1β and IRG1 at mRNA and protein levels (Fig. 1d,e). Strikingly, treatment with ERG240 (3 h, 20 mM) resulted in a dramatic reduction in IRG1 mRNA levels but not HIF1A and IL1B in LPS-stimulated cells (Fig. 1d). These results were also confirmed at the IRG1 protein levels in LPS and ERG240-treated cells (Fig. 1e). Consequently, the ERG240-mediated reduction in IRG1 levels resulted in decreased itaconate levels measured by GC/MS (Fig. 1f). ERG240 had comparable inhibitory effect on IRG1 expression in TNF-stimulated human macrophages (Supplementary Fig. 1A) and long-term exposure (24 h) to LPS and ERG240 resulted in reduced IL-1β levels (Supplementary Fig. 1B), suggesting a temporal effect of ERG240 on IL-1β. Furthermore, short-term exposure of ERG240 significantly reduced TNF and NOS2 but not IL6 and PTGS2 expression levels in human macrophages (Supplementary Fig. 1F). ERG240 did not affect cell viability at 3 and 8 h LPS stimulation in neither control nor LPS-treated human macrophages (Supplementary Fig. 1E). Importantly, the effect of BCAT1 inhibition on IRG1 expression and itaconate levels was ascertained in macrophages stimulated with LPS in a BCAA-deprived media (Supplementary Fig. 1C and D), confirming the requirement for an enzymatically active BCAT1 for its inhibition of IRG1-mediated itaconate production.
Next, we measured the effect of BCAT1 inhibition on oxygen consumption and glycolysis in hMDMs. The mitochondrial respiration and glycolytic metabolism were assessed by measurement of oxygen consumption rate (OCR) and extracellular acidification rates (ECAR), respectively. The bioenergetic profiles were determined by sequential use of oligomycin (mitochondrial ATP synthase inhibitor), FCCP (protonophore and uncoupler of ATP synthesis from mitochondrial respiration), and a combination of rotenone, antimycin A and 2-deoxyglucose (2-DG) that allows the inhibition of mitochondrial respiration and glycolysis. LPS treatment alone resulted in increased OCR (basal respiration, estimated ATP production and maximal respiration) and ECAR (glycolysis and glycolytic capacity) in hMDMs (Supplementary Fig. 2). Treatment of LPS-stimulated hMDMs with ERG240 resulted in decreased OCR and ECAR (Fig. 2a,b). This translated into a significantly reduced basal respiration, estimated ATP production, maximal respiration, glycolysis and glycolytic capacity (Fig. 2a,b). In keeping with the decreasing effect of ERG240 on oxygen consumption and glycolysis, siRNA-mediated inhibition of BCAT1 mRNA levels resulted in a significant decrease in OCR and ECAR levels (Fig. 2c,d). We also measured citrate by GC/MS in hMDMs as the breakpoint TCA metabolite in M(LPS)18 and found a relative increase in citrate levels following LPS stimulation, which was further significantly increased in the presence of ERG240 (Supplementary Fig. 3A), suggesting that the effect of BCAT1 inhibition on the broken TCA cycle in human macrophages lies predominantly downstream citrate at the IRG1 site with a relatively minor effect through inhibition of BCAA transamination and reduction in the amount of acetyl-coA entering the cycle (Supplementary Fig. 3B). To confirm the pharmacological inhibition with ERG240, we used RNAi on human BCAT1 to measure IRG1 protein levels and itaconate (Fig. 3a). The experiment showed consistent knock down of BCAT1 associated with reduced IRG1 levels in three independent donors (Fig. 3a). In keeping with the pharmacological blockade, BCAT1 knockdown resulted in reduced IRG1 and itaconate levels in hMDMs (Fig. 3b).
ERG240 suppresses in vivo production of IRG1/itaconate
To confirm the inhibitory effect of ERG240 on IRG1-mediated itaconate production in M(LPS) in vivo, we injected C57BL/6 mice with either LPS (1.5 mg kg−1) or LPS in conjunction with ERG240 (500 mg kg−1), and harvested peritoneal macrophages 24 h later. These results showed a reduction in Irg1 mRNA and protein levels associated with a significant decrease in itaconate (Fig. 4a–c). Itaconate was equally decreased when ERG240 was administered 3 h after the first LPS injection (Fig. 4c).
To gain more insights into the effect of ERG240 on macrophage polarization, we performed RNA-sequencing (RNA-seq) in vehicle, LPS and LPS+ERG240 treated peritoneal macrophages at the time of collection (24 h). When the top 100 (97 annotated, 28 up-regulated and 72 down-regulated) differentially expressed transcripts (10−310<FDR <10−73) between LPS and LPS+ERG240 treated groups were analysed by STRING for high confidence protein-protein interactions (interaction score=0.7), this led to the identification of an interferon (IFN)-inducible GTPase pathway (PFAM protein domains FDR=6.97 × 10−6; Fig. 4d). Interestingly, all of the 25 genes belonging to the IFN-inducible GTPase signature are down-regulated together with M1-like markers (Nos2, Il6), while M2-like and extracellular matrix transcripts are up-regulated in the peritoneal macrophages obtained from ERG240-treated mice (Fig. 4e-g). Mrc1, Klf4 and Cd36 were previously described as alternative (M2-like) macrophage activation markers24,25,26, and Fragments Per Kilobase of transcript per Million fragments mapped (FPKM) values for these transcripts show significant up-regulation in ERG240-treated macrophages (Fig. 4g). Ifit and Gbp gene families’ expression values are also illustrated in Fig. 4g. Furthermore, the extracellular matrix enrichment is striking (Cellular component GO: extracellular space, FDR=1.04 × 10−8) among the 28 most significantly up-regulated genes. FPKM values. FPKM values for Fn1, Mmp9, Gsn and Spp1 are shown in Fig. 4g. Taken together, these results show that long-term ERG240 exposure in LPS-injected mice is associated with a reduced proinflammatory and increased anti-inflammatory and repair transcriptome signature in peritoneal macrophages.
ERG240 reduces the severity of immune-mediated inflammation
First, a dose-escalating toxicity study was performed and indicated that no major adverse effects were associated with the oral or intraperitoneal administration of ERG240 when the drug was given at doses up to 2 g kg−1. A subsequent pharmacokinetics study revealed that ERG240 showed excellent bioavailability with a relatively short half-life in plasma suggesting either rapid absorption or clearance of the drug (Tables 1 and 2).
The reduced proinflammatory transcriptome signature following treatment with ERG240 in the peritoneal macrophages of LPS-injected mice led us hypothesize that BCAT1 blockade could be beneficial in broader inflammatory conditions. To test the potential anti-inflammatory effects of ERG240 in a macrophage-dependent model of immune complex-driven tissue inflammation, we assessed the effect of ERG240 administration in the well-established nephrotoxic nephritis (NTN) model utilizing the uniquely susceptible Wistar-Kyoto (WKY) rat strain27,28. The macrophage-dependent kidney inflammation observed in this model resembles the immune complex mediated glomerular damage in systemic lupus erythematosus referred as lupus nephritis29. Oral administration of ERG240 during 10 days following NTN induction resulted in significantly reduced glomerular crescent formation, proteinuria, and serum creatinine (Fig. 5a), demonstrating the ability of ERG240 to reduce the severity of inflammation in a highly reproducible and severe model of antibody-mediated kidney inflammation. Treatment with ERG240 did not interfere with the deposition of the nephrotoxic serum to the glomerular basement membrane. Quantitative immunofluorescence for collagen type I alpha I (Cola1) in nephritic kidneys (day 28) showed a significant down-regulation in the ERG240-treated animals (Fig. 5b), which was in accordance with the reduced Sirius red staining and Col1a1 mRNA levels in the renal cortex (Fig. 5b). These results showed that treatment with ERG240 resulted in reduced interstitial fibrosis in the NTN model of crescentic glomerulonephritis.
In addition to NTN, macrophages play a central role in the pathogenesis of rheumatoid arthritis (RA) where they infiltrate synovial membranes30,31, contributing to chronic inflammation. Bone erosion in RA has also been linked to monocytes/macrophages since these cells are known to differentiate into osteoclasts in the presence of M-CSF and RANKL32. Hence, the effect of Bcat1 inhibition on collagen-induced arthritis (CIA) in mice was examined prophylactically, by administering 720 mg kg−1 ERG240 at the time of the booster injection 21 days after the initial immunization, and therapeutically, by administering 1000, mg kg−1 ERG240 after arthritis was established. The results show that oral administration of ERG240 alleviates the severity of CIA (Fig. 5c). Analysis of the sera obtained before immunization and at the end of the experiment validated the presence of antibodies against type II collagen and confirmed that ERG240 did not interfere with antibody production (Supplementary Figure 4).
Following completion of CIA, excised paws were analysed for presence of inflammation, pannus formation, cartilage damage and bone resorption by immunohistochemistry (Fig. 5c). Despite its moderate effect on oedema formation, ERG240 markedly reduced the progression of the disease by decreasing inflammation by 53%, cartilage degradation by 74% and pannus formation and bone erosion by 86% (Fig. 5c). We found a significant decrease in joint oedema with ERG240 administration only in the fore paws of the animals in the therapeutic study (Supplementary Fig. 5). We further measured serum Tnf and Tnfsf11 (RANKL) levels in CIA mice and found diminished levels of these circulating markers upon ERG240 treatment (Supplementary Fig. 6).
ERG240 treatment reduces macrophage infiltration in vivo
We first evaluated the potential effect of ERG240 on macrophage migration and showed that ERG240 suppresses BMDM migration in a dose-dependent manner and with an IC50 value of ∼5–10 mM (Fig. 6a). Inhibition of migration was not incidental to a cell death event as ERG240 did not affect cell numbers after treatment of BMDM for 24 h (Fig. 6a). To further investigate the mechanisms of action of ERG240 in vivo, excised paws were tested for the presence of F4/80 by immunohistochemistry and we found that there was a significant reduction in F4/80+ macrophages in the joints following ERG240 treatment (Fig. 6b). Similarly to what we observed in CIA, macrophage infiltration was significantly reduced in nephrotoxic nephritis model as the glomerular infiltration of CD68 (ED1)-positive cells were significantly decreased in ERG240-treated animals (Fig. 6c).
A growing number of studies suggest that cellular metabolism, immune response and inflammation are intimately linked processes under common, evolutionary conserved regulatory pathways33. The majority of the reports supporting the above concept focus on glucose, glutamine and lipid metabolism or document an association between deregulated metabolism and immune pathology7,34,35. BCAA catabolism provides generally a mechanism for regulating the levels of leucine, isoleucine and valine, whose excess has been previously associated with neural dysfunction36,37. Up-regulation of Bcat1 under pathological conditions may serve to regulate the local levels of BCAAs and especially leucine, which is known to activate the mammalian target of rapamycin complex 1 (mTORC1). In CD4+ T cells, Bcat1 regulates mTORC1 signalling and glycolytic metabolism, highlighting the important role of leucine metabolism38. BCAA transamination serves also as the source of amino groups for the synthesis of non-essential amino acids such as glutamine and alanine39. BCAT1 is considered as a secondary target of gabapentin, a common antiepileptic and antinociceptive drug that is known to bind with high affinity to a2δ−1 and a2δ−2 subunits of voltage-activated calcium channels40. However, gabapentin has been previously shown to display no apparent anti-inflammatory effects41. The role of BCAA catabolism in macrophage function is poorly described. In a recent report, Meiser et al. observed an increased uptake of leucine in RAW264.7 murine macrophage cell line stimulated with LPS both in normoxia and hypoxia42, suggesting that LPS stimulation in macrophages results in an increase of BCAA as an alternative carbon source to glucose and glutamine42.
Here, we describe BCAT1 as the predominantly expressed BCAT isoform in human macrophages. We found that pharmacological blockade of BCAT1 resulted in decreased oxygen consumption and glycolysis in LPS-treated macrophages. We focused on early LPS activation (3 h) because major transcriptome changes involving primary response genes occur 2-4 h following LPS stimulation in macrophages13,43 and monocytes44 with some key Toll-like receptor4-dependent markers such as Hif1a (ref. 45), Il1b (refs 43, 46) and Irg1 (refs 19, 47) detected as a rapid transcriptional activation response. All these early response markers were found to be tightly regulated by TCA in M(LPS)14,18, in particular Irg1 and its enzymatic product, itaconate, which accumulates following 8–10 h LPS stimulation in primary mouse macrophages48 and RAW264.7 cells19. Itaconate is a bactericidal metabolite that could have distinct roles depending on the pathological context49. Besides its antimicrobial effects, itaconate has also been proposed as an inhibitor of substrate level phosphorylation through its effect on succinate-CoA ligase in RAW-264.7 cells50. Furthermore, itaconic acid has been shown to inhibit SDH48,51,52 and a recent study linked IRG1-itaconic acid production to modulation of M2-like macrophage polarization in the revascularization of ischaemic muscle53. Investigating pathways upstream and downstream IRG1/itaconate are the focus of recent studies48,52,54 and will facilitate understanding the gene-metabolite links in activated macrophages. This will be crucial in determining the mechanisms by which BCAT1 regulate itaconate synthesis.
Metabolism in M(LPS) macrophages is characterized by high levels of glycolysis, a fragmented TCA and relatively high levels of some of its intermediates (succinate, citrate and itaconate). By contrast, metabolism in M2 (or M(IL-4)) corresponds to high levels of fatty acid oxidation (FAO) and oxidative phosphorylation even though the exact role of FAO in macrophage activation states require further clarification55. Our findings indicating (i) reduced ECAR levels as a result of BCAT1 inactivation in human macrophages and (ii) anti-inflammatory effects of ERG240, reversing the M1-like transcriptome in mouse peritoneal macrophages, argue in favour of the involvement of BCAA in M1/proinflammatory macrophage function. Although the metabolic response of murine macrophages and dendritic cells to LPS stimulation has been thoroughly explored15,17, the bioenergetic profile of human macrophages is not well described and recent reports suggest that culturing conditions (M-CSF versus GM-CSF) play an important role in their metabolic reprogramming56. Here, we report that early LPS stimulation of human macrophages resulted in an increase of ECAR, which is indicative of increased glycolytic rate and is consistent with previous results obtained in murine bone marrow derived and peritoneal macrophages15.
The recently discovered discontinuity in the TCA cycle at the Isocitrate Dehydrogenase 1 (IDH1) level, resulting in a flux re-direction towards citrate/aconitate has important implications in terms of proinflammatory macrophage activation18. This metabolic axis enables large production of fatty acids and itaconate and our results suggest that leucine catabolism through BCAT1 is a regulator of IRG1-dependent macrophage activation. It is likely that BCAT1 regulates the production of fatty acids and, in accordance with this, its expression levels in human adipose tissue were found to correlate with weight regain, making BCAT1 expression the best marker for this phenotype57. Likewise, a down-regulation in BCAT1 expression was found to correlate with a dietary weight loss program58.
Here, we demonstrate that Bcat1 is a druggable target for controlling inflammation in two distinct chronic inflammatory diseases characterized by antibody-mediated end-organ damage with macrophage infiltration. The rat model of crescentic glomerulonephritis in the WKY strain is dependent on macrophage infiltration and activation in response to glomerular deposition of immunoglobulin21,59,60. Primary macrophages from the WKY strain show a genetically determined Hif-1α-mediated glycolytic transcriptome signature during their differentiation61, suggesting a genetically determined metabolic state that could explain their unique susceptibility to glomerular inflammation. In RA, macrophages have been described as the primary pathogenic drivers of the disease with fibroblasts, T and B lymphocytes playing secondary roles62. The protective effect of Bcat1 inhibition in two LPS-independent inflammatory models suggest that Bcat1 may have its regulatory role on macrophages activated with different proinflammatory stimuli and this is consistent with the in vitro observation that ERG240 inhibits Irg1 mRNA levels in TNF-activated human MDMs. Nevertheless, the role of macrophage-derived BCAT1 in inflammatory models can only be firmly established by using conditional targeted gene deletion of Bcat1 in monocytes/macrophages.
The druggability of Bcat1 in autoimmune disease was shown with the utilization of ERG240, a novel Bcat1 inhibitor. ERG240 had an overall favourable bioavailability profile with no observable toxicity and, although administered at high concentrations, it showed promise as a therapeutic agent for autoimmune diseases. While we acknowledge possible off-target effects of ERG240 usage in vivo, the specificity towards Bcat1 and not its mitochondrial isoform (Bcat2) is supported by the contrasting physiological consequences observed as a result of disrupting Bcat1 and Bcat2 genes, individually. Bcat2−/− mice show 50-fold elevation in plasma BCAA concentrations, which leads to organ hypertrophy63, seizures and death (unpublished observation) if the animals are not offered a low-branched-chain amino acid diet64. In contrast, Bcat1−/− mice have normal plasma BCAA concentrations when fed a standard rodent chow38, suggesting that if there were any non-specific pharmacological effects of ERG240 towards Bcat2 in vivo, this would possibly lead to notable pathological consequences which were not observed in neither of the disease models in rats and mice.
In summary, we show that blocking Bcat1 results in therapeutic outcome in two independent models of autoimmune diseases with end-organ damage (joints and glomeruli). We show that macrophages express relatively high amounts of Bcat1 and blocking its activity inhibit Irg1/itaconate levels in human macrophages, and in vivo. Furthermore, Bcat1 inhibition is also associated with reduced macrophage migration in vitro and decreased tissue infiltration in arthritis and glomerulonephritis. Thus, macrophage BCAT1 and its ability to interfere with metabolic reprogramming is an attractive pharmacological target for the treatment of chronic inflammatory diseases.
DBA/1 mice (male, 6–8 weeks old) used in CIA experiments were purchased from Harlan Sprague Dawley Inc. WKY (male, 16-weeks old, WKY/NCrl) rats used for NTN experiments were purchased from Charles River UK. All mice and rats were used straight from the source by housing them until the appropriate experimental age. All procedures were performed in accordance to institutional guidelines and procedures approved by the UK Home Office (United Kingdom Animals Scientific ProceduresAct, 1986) or followed the requirements of the United States of America Animal Welfare Act (1966) and the guidelines outlined in the Guide for the Care and Use of Laboratory Animals, 8th Edition (2011).
Cells and reagents
Human monocyte-derived macrophages were differentiated from buffy coats from healthy donors using gradient separation (Histopaque 1077, Sigma) and adhesion purification. Following Histopaque separation, peripheral blood mononuclear cells were re-suspended in RPMI (Life Technologies) and monocytes were purified by adherence for 1 h at 37 °C, 5% CO2. The monolayer was washed 6 times with HBSS to remove non-adherent cells and monocytes were matured for 5 days in RPMI containing 100 ng ml−1 M-CSF (PeproTech, UK) and 10% Fetal Calf Serum (FCS, Labtech International). Macrophage purity was confirmed by immunohistochemical assessment of CD68 and >99% cells were CD68+. For migration assays, C57BL/6 mouse bone marrow derived macrophages (BMDMs) were purchased from Cell Biologics. The cells were cultured in culture media according to manufacturer’s instructions. The phenotype of the BMDMs was verified by determining the expression of macrophage marker CD68 by whole cell ELISA. The migration assay is detailed in Supplementary Methods. For BCAA deprivation assay, RPMI 1640 containing L-Glutamine but no BCAAs was used.
Lipopolysaccharide was purchased from Sigma (LPS; Escherichia coli serotype 0111:B4; Cat No L4391) and the antibodies used in Western Blot analyses are as follows: IL-1β (Cell Signaling Technologies, #12703; D3U3E, dilution 1/1,000), IRG1 (Abcam, ab122624, rabbit polyclonal, dilution, 1/1,000), HIF-1α (NOVUS biological, NB100-449, rabbit polyclonal, dilution 1/2,000), ACTB (Santa Cruz, sc-47778, β-Actin (C4), mouse monoclonal, dilution 1/2,000), BCAT1 (anti Bcat1 raised in rabbit, produced in Susan Hutson’s laboratory4,65, 8382, dilution 1/120,000), TUBB (Cell Signaling Technologies, #2128, rabbit monoclonal, dilution 1/30,000).
Western blot and RNA analysis
hMDMs were lysed in Laemmli sample buffer supplemented with protease inhibitors and resolved by SDS-PAGE, transferred into PVDF membranes, and subjected to immunoblotting with the primary antibodies described above and secondary detection antibodies. The probed proteins were detected using SuperSignal West Femto Chemiluminescent Substrate (Thermo Fisher Scientific Inc., Rockford, IL). Uncropped scans of western blots presented in the main and Supplementary Figures are provided in Supplementary Fig. 8. qRT-PCR reactions were performed using the Viaa 7 Real-Time PCR system (Life technologies). A two-step protocol was used beginning with cDNA synthesis with iScript select (Bio-Rad) followed by PCR using Brilliant II SYBR Green QPCR Master Mix (Agilent). A total of 10 ng of cDNA per sample was used. Viia 7 RUO Software was used for the determination of Ct values. Results were analysed using the comparative Ct method and each sample was normalized to the indicated reference gene, to account for any cDNA loading differences. For RNAi, hMDMs were re-plated in six-well plates (1 × 106 cells per well) in RPMI (Invitrogen) overnight and transfected with siGENOME SMARTpool for human BCAT1 (100 nM, Dharmacon SMART pool) or non-targeting siRNA pool as the scrambled control siRNA using Dharmafect 1 (1:50, Dharmacon) as a transfection reagent in OPTIMEM medium (Invitrogen). Following 8 h incubation with OPTIMEM media containing either BCAT1 or non-targeting siRNA, the culture media was washed and cells were further cultured for 48 h in presence of RPMI media containing M-CSF and FCS. Cells were then used either for extracellular flux analysis (see below) or IRG1, BCAT1, ACTB and TUBB Western Blot analysis. Primer sequences for siRNA and qRT-PCR are provided in Supplementary Fig. 7.
Real-time measurements of OCR and ECAR were performed using a Seahorse XF96 Extracellular Flux Analyzer (Seahorse Bioscience). hMDMs cultured for 5 days in presence of RPMI containing M-CSF (100 ng ml−1) and FCS (10%) were washed and incubated with either ERG240 or LPS or a combination of both for 3 h. Cells were then washed, re-suspended using the non-enzymatic cell dissociation buffer (Sigma) and 5 × 105 hMDMs were seeded as a monolayer in a 96-well microplate containing XF Assay Modified DMEM. Glucose and different metabolic drugs were injected during real-time measurements of OCR and ECAR. Basal respiration was calculated as the last measurement before addition of oligomycin – non mitochondrial respiration (minimum rate measurement after Rot/AntA). Estimated ATP production designates the last measurement before addition of oligomycin – minimum rate after oligomycin. Maximal respiration is shown as the maximum rate measurement after addition of FCCP – non mitochondrial respiration (minimum rate measurement after Rot/AntA). Glycolysis refers to ECAR values before the addition of oligomycin – ECAR values before the addition of glucose. Glycolytic capacity shows ECAR values following addition of oligomycin – ECAR values before the addition of glucose. In RNAi experiments, hMDMs transfected with either BCAT1 siRNA or control (non-targeting) siRNA were seeded in a 96-well microplate, LPS-stimulated (100 ng ml−1, 3 h) and real-time measurements of OCR and ECAR were recorded, as described above.
Mouse i.p. injection of LPS and ERG240
The procedure was performed by Washington Biotechnology Inc. (Baltimore, MD). 6–8 week old female C57BL6 mice were administered ERG240 (500 mg kg−1) by intraperitoneal route 30 min before the i.p. LPS injection (from E. coli strain 055:B5, 1.5 mg kg−1). Same dose of ERG240 was administered a second time, 8 h following the first injection of LPS. Peritoneal macrophages were harvested 24 h after the LPS injection and results from three groups (vehicle, LPS only, LPS+ERG240; n=6 mice in each group) were analysed in terms of IRG1 and itaconate level measurements.
Macrophage itaconate and citrate measurements by GC/MS
For itaconate, cells pellets containing 1.5 × 106 hMDMs were quenched with 400 μl of methanol, previously chilled at −20 °C. After addition of 400 μl of cold (4 °C) ultrapure water, cell pellets were vortexed briefly and then allowed to stand for 10 min on ice. Following addition of 400 μl of GC/MS grade dichloromethane, previously chilled at −20 °C, samples were vortexed again and centrifuged at 14,000g for 10 min at 4 °C. The aqueous layer of the samples was collected and lyophilized. Dried materials were dissolved in 40 μl of MOX solution, which is prepared by allowing 20 mg of methoxamine HCl (Sigma Aldrich) to dissolve in 500 μl pyridine for 30 min at room temperature. Metabolites dissolved in MOX were incubated at 45 °C for 60 min, derivatized by adding 60 μl of N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSFTA, Mecherey-Nagel) and incubated at 45 °C for 30 min. After a brief centrifugation, derivatized metabolites were transferred into clean GC/MS vials and analysed for the presence of itaconic acid (itaconate) using a 7890A GC System (Agilent Technologies) equipped with an Agilent J&W DB-5ms column (30 m, 0.25 mm, 0.25 μm), connected to an 5975C Triple-Axis MS Detector (Agilent Technologies) and outfitted with an autosampler. The MS source was held at 230 °C and the quadrupole at 150 °C. The GC oven temperature was held at 50 °C for 5 min, increased to 240 °C at a rate of 10 °C min−1, held at 240 °C for 5 min and increased to 300 °C at a rate of 10 °C min−1. A volume of 1 μl was injected in the system in a 1:3 split mode. The amount of itaconate present in the sample was determined after peak integration. For citrate, the frozen cell pellets were re-suspended in ice-cold ethanol-phosphate buffer. Cells were lysed by three freeze-thaw cycles and sonication. Samples were centrifuged and the supernatant was used for further analysis. The GC/MS was performed by Biocrates (BIOCRATES Life Sciences AG, Innsbruck, Austria) and the citrate quantification was performed using their commercially available Kit plates, according to the manufacturer’s instructions. After derivatization to its corresponding methoxime-trimethylsilyl (MeOx-TMS) derivative, citrate levels were determined by GC/MS using Agilent 7890 GC/5975 MSD (Agilent, Santa Clara, USA). Pretreated samples were evaporated to complete dryness and subjected to a two-step methoximation-silylation derivatization. N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) was used as silylation reagent. Split injection was performed and chromatograms were recorded in selected ion monitoring mode. External standard calibration curves and ten internal standards were used to calculate concentrations of individual energy metabolites. Data were quantified using the appropriate MS software (Agilent, Masshunter) and imported into Biocrates MetIDQ software for further analysis.
In vivo pharmacokinetics
The experiment was performed by Eurofins Panlabs Inc. Briefly, fasting pharmacokinetic parameters were obtained after administration of 125 mg kg−1 i.v. or 500 mg kg−1 p.o ERG240 to ICR mice and blood collection at 3, 10, 30, 60, 120 and 240 min (i.v. dosing) or 10, 30, 60, 120, 240 and 360 min (p.o. dosing). Each animal was subjected to a single blood draw and 3 animals per time point were used. Parameters were obtained from non-compartmental analysis of the plasma data using WinNonlin. Experimental details are provided in Supplementary Methods.
Total RNA was extracted from hMDMs and mouse peritoneal macrophages using Trizol (Invitrogen) according to manufacturer's instructions with an additional purification step by on-column DNase treatment using the RNase-free DNase Kit (Qiagen) to ensure elimination of any genomic DNA. The integrity and quantity of total RNA was determined using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific) and Agilent 2100 Bioanalyzer (Agilent Technologies). In total 500 ng of total RNA was used to generate RNA-seq libraries using TruSeq RNA sample preparation kit (Illumina) according to the manufacturer's instructions (see Supplementary Methods for detailed library preparation, mapping and sequencing protocols).
Collagen-induced arthritis in mice
DBA/1 mice (6–8 weeks old from Harlan Sprague Dawley Inc.) were injected s.c. with 100 μg of bovine type II collagen emulsified in complete Freund’s adjuvant (Sigma). At day 21 following primary immunization, the mice were given a booster injection of 100 μg of bovine type II collagen emulsified in incomplete Freund’s adjuvant. The prophylactic arm of the study involved water-treated (vehicle control, n=6) and ERG240-treated (720 mg kg−1, o.p., qdx5, 3 weeks, n=10) animals, while the therapeutic arm of the study involved water-treated (vehicle control, n=10) and ERG240-treated (1,000 mg kg−1, o.p., qdx5, 4 weeks, n=10) animals. Parafin-embedded paws were sectioned at ∼5 μm. One slide from each paw was stained with hematoxylin and eosin (H&E) and served as a reference, while four additional slides were stained using antibodies against F4/80 (AbD Serotec). The expression of the above markers in the joints of the preserved CIA tissues was scored according to the following scale: 0=no staining; 1=minimal; 2=mild; 3=moderate; 4=marked; and 5=diffuse and severe staining (see Supplementary Methods for detailed histological evaluation of CIA tissues). Serum levels of TNF and RANKL were analysed using relevant ELISA kits from Biolegend (TNF-α) and Abcam (RANKL) according to the manufacturers’ instructions. Details for determining the presence of antibodies against type II collagen are provided in Supplementary Methods.
Nephrotoxic nephritis in the WKY rat
Nephrotoxic nephritis (NTN) was induced in twelve week old male Wistar-Kyoto (WKY) rats by intravenous injection of 0.1 ml of nephrotoxic serum (NTS), as previously described66. Five WKY rats received ERG240 (250 mg kg−1) orally in 0.1% carboxymethylcellulose once a day for a duration of 10 days whereas the vehicle treated WKY rats had carboxymethylcellulose only for the same period. Nine days following NTS injection, urine was collected by placing rats in metabolic cages for 24 h with free access to food and water. Proteinuria was determined by the sulphosalicylic acid test and serum creatinine by the Creatinine Assay Kit (Abcam). After 10 or 28 days of NTN induction, rats were culled and the kidney was formalin fixed and paraffin embedded for immunohistochemistry. To quantify the number of macrophages infiltrating the glomeruli, formalin-fixed paraffin-embedded kidney sections were stained with mouse monoclonal antibody to ED-1 (Serotec, Oxford, UK), followed by an HRP-labelled anti-mouse polymer development system (Dako Ltd, UK). For the evaluation of total collagen, renal tissues were stained with Sirius red. For Col1a1 immunofluorescence, sections were blocked with BSA, incubated with the Col1a1 primary antibody raised in goat (Southern Biotech, 1/40 dilution), washed and re-incubated with secondary antibody (donkey anti-goat-FITC, 1/100 dilution, Abcam). For ED-1 staining, the cellular infiltrates in 20 consecutive glomeruli were quantified using an automated image analysis software (ImagePro Plus, Media Cybernetics, Bethesda, MD) and expressed as a percentage of total glomerular cross sectional area.
Data are presented as mean±s.e.m. and analysed using GraphPad Prism software (version 7.02; GraphPad). The Mann–Whitney or Student’s t-tests were used for comparison of two groups. Differences in percentage of itaconate in human samples were tested for significance using a one-sample-t-test. One-way or two-way ANOVA analyses (followed by Tukey’s or Dunnet’s or Sidak’s multiple comparison tests) were used for comparative analyses of three or more groups.
Mouse sequence data that support the findings of this study have been deposited in the European Nucleotide Archive with the primary accession code PRJEB20215. The authors declare that all other data supporting the findings of this study are available within the paper and its Supplementary Information files or are available from the corresponding authors on request.
How to cite this article: Papathanassiu, A. E. et al. BCAT1 controls metabolic reprogramming in activated human macrophages and is associated with inflammatory diseases. Nat. Commun. 8, 16040 doi: 10.1038/ncomms16040 (2017).
Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Brosnan, J. T. & Brosnan, M. E. Branched-chain amino acids: enzyme and substrate regulation. J. Nutr. 136, 207S–211S (2006).
Tom, A. & Nair, K. S. Assessment of branched-chain amino Acid status and potential for biomarkers. J. Nutr. 136, 324S–330S (2006).
Hutson, S. M., Lieth, E. & LaNoue, K. F. Function of leucine in excitatory neurotransmitter metabolism in the central nervous system. J. Nutr. 131, 846S–850S (2001).
Sweatt, A. J. et al. Branched-chain amino acid catabolism: unique segregation of pathway enzymes in organ systems and peripheral nerves. Am. J. Physiol. Endocrinol. Metab. 286, E64–E76 (2004).
Tonjes, M. et al. BCAT1 promotes cell proliferation through amino acid catabolism in gliomas carrying wild-type IDH1. Nat. Med. 19, 901–908 (2013).
Galvan-Pena, S. & O'Neill, L. A. Metabolic reprograming in macrophage polarization. Front. Immunol. 5, 420 (2014).
Ganeshan, K. & Chawla, A. Metabolic regulation of immune responses. Annu. Rev. Immunol. 32, 609–634 (2014).
O'Neill, L. A. & Pearce, E. J. Immunometabolism governs dendritic cell and macrophage function. J. Exp. Med. 213, 15–23 (2016).
Rodriguez-Prados, J. C. et al. Substrate fate in activated macrophages: a comparison between innate, classic, and alternative activation. J. Immunol. 185, 605–614 (2010).
Mosser, D. M. & Edwards, J. P. Exploring the full spectrum of macrophage activation. Nat. Rev. Immunol. 8, 958–969 (2008).
Murray, P. J. et al. Macrophage activation and polarization: nomenclature and experimental guidelines. Immunity 41, 14–20 (2014).
Xue, J. et al. Transcriptome-based network analysis reveals a spectrum model of human macrophage activation. Immunity 40, 274–288 (2014).
Medzhitov, R. & Horng, T. Transcriptional control of the inflammatory response. Nat. Rev. Immunol. 9, 692–703 (2009).
Tannahill, G. M. et al. Succinate is an inflammatory signal that induces IL-1beta through HIF-1alpha. Nature 496, 238–242 (2013).
Haschemi, A. et al. The sedoheptulose kinase CARKL directs macrophage polarization through control of glucose metabolism. Cell Metab. 15, 813–826 (2012).
Freemerman, A. J. et al. Metabolic reprogramming of macrophages: glucose transporter 1 (GLUT1)-mediated glucose metabolism drives a proinflammatory phenotype. J. Biol. Chem. 289, 7884–7896 (2014).
Everts, B. et al. TLR-driven early glycolytic reprogramming via the kinases TBK1-IKKvarepsilon supports the anabolic demands of dendritic cell activation. Nat. Immunol. 15, 323–332 (2014).
Jha, A. K. et al. Network integration of parallel metabolic and transcriptional data reveals metabolic modules that regulate macrophage polarization. Immunity 42, 419–430 (2015).
Michelucci, A. et al. Immune-responsive gene 1 protein links metabolism to immunity by catalyzing itaconic acid production. Proc. Natl Acad. Sci. USA 110, 7820–7825 (2013).
O'Neill, L. A. A broken krebs cycle in macrophages. Immunity 42, 393–394 (2015).
Kang, H. et al. Kcnn4 is a regulator of macrophage multinucleation in bone homeostasis and inflammatory disease. Cell Rep. 8, 1210–1224 (2014).
Yennawar, N., Dunbar, J., Conway, M., Hutson, S. & Farber, G. The structure of human mitochondrial branched-chain aminotransferase. Acta. Crystallogr. D Biol. Crystallogr. 57, 506–515 (2001).
Cooper, A. J., Conway, M. & Hutson, S. M. A continuous 96-well plate spectrophotometric assay for branched-chain amino acid aminotransferases. Anal. Biochem. 308, 100–105 (2002).
Huang, S. C. et al. Cell-intrinsic lysosomal lipolysis is essential for alternative activation of macrophages. Nat. Immunol. 15, 846–855 (2014).
Liao, X. et al. Kruppel-like factor 4 regulates macrophage polarization. J. Clin. Invest. 121, 2736–2749 (2011).
Stein, M., Keshav, S., Harris, N. & Gordon, S. Interleukin 4 potently enhances murine macrophage mannose receptor activity: a marker of alternative immunologic macrophage activation. J. Exp. Med. 176, 287–292 (1992).
Behmoaras, J. et al. Jund is a determinant of macrophage activation and is associated with glomerulonephritis susceptibility. Nat. Genet. 40, 553–559 (2008).
Tam, F. W. et al. Development of scarring and renal failure in a rat model of crescentic glomerulonephritis. Nephrol. Dial. Transpl. 14, 1658–1666 (1999).
Aitman, T. J. et al. Copy number polymorphism in Fcgr3 predisposes to glomerulonephritis in rats and humans. Nature 439, 851–855 (2006).
Kinne, R. W., Stuhlmuller, B. & Burmester, G. R. Cells of the synovium in rheumatoid arthritis. Macrophages. Arthritis. Res. Ther. 9, 224 (2007).
Swirski, F. K. et al. Identification of splenic reservoir monocytes and their deployment to inflammatory sites. Science 325, 612–616 (2009).
Takayanagi, H. Osteoimmunology: shared mechanisms and crosstalk between the immune and bone systems. Nat. Rev. Immunol. 7, 292–304 (2007).
Hotamisligil, G. S. & Erbay, E. Nutrient sensing and inflammation in metabolic diseases. Nat. Rev. Immunol. 8, 923–934 (2008).
Maciver, N. J. et al. Glucose metabolism in lymphocytes is a regulated process with significant effects on immune cell function and survival. J. Leukoc. Biol. 84, 949–957 (2008).
Newsholme, P. & Newsholme, E. A. Rates of utilization of glucose, glutamine and oleate and formation of end-products by mouse peritoneal macrophages in culture. Biochem. J. 261, 211–218 (1989).
Hutson, S. M. The case for regulating indispensable amino acid metabolism: the branched-chain alpha-keto acid dehydrogenase kinase-knockout mouse. Biochem. J. 400, e1–e3 (2006).
Hutson, S. M. et al. Role of branched-chain aminotransferase isoenzymes and gabapentin in neurotransmitter metabolism. J. Neurochem. 71, 863–874 (1998).
Ananieva, E. A., Patel, C. H., Drake, C. H., Powell, J. D. & Hutson, S. M. Cytosolic branched chain aminotransferase (BCATc) regulates mTORC1 signaling and glycolytic metabolism in CD4+ T cells. J. Biol. Chem. 289, 18793–18804 (2014).
Hull, J. et al. Distribution of the branched chain aminotransferase proteins in the human brain and their role in glutamate regulation. J. Neurochem. 123, 997–1009 (2012).
Taylor, C. P. Mechanisms of analgesia by gabapentin and pregabalin--calcium channel alpha2-delta [Cavalpha2-delta] ligands. Pain 142, 13–16 (2009).
Lu, Y. & Westlund, K. N. Gabapentin attenuates nociceptive behaviors in an acute arthritis model in rats. J. Pharmacol. Exp. Ther. 290, 214–219 (1999).
Meiser, J. et al. Pro-inflammatory macrophages sustain pyruvate oxidation through pyruvate dehydrogenase for the synthesis of itaconate and to enable cytokine expression. J. Biol. Chem. 291, 3932–3946 (2016).
Ramirez-Carrozzi, V. R. et al. A unifying model for the selective regulation of inducible transcription by CpG islands and nucleosome remodeling. Cell 138, 114–128 (2009).
Fairfax, B. P. et al. Innate immune activity conditions the effect of regulatory variants upon monocyte gene expression. Science 343, 1246949 (2014).
Peyssonnaux, C. et al. Cutting edge: essential role of hypoxia inducible factor-1alpha in development of lipopolysaccharide-induced sepsis. J. Immunol. 178, 7516–7519 (2007).
Suzuki, T. et al. Comprehensive gene expression profile of LPS-stimulated human monocytes by SAGE. Blood 96, 2584–2591 (2000).
Li, Y. et al. Immune responsive gene 1 (IRG1) promotes endotoxin tolerance by increasing A20 expression in macrophages through reactive oxygen species. J. Biol. Chem. 288, 16225–16234 (2013).
Lampropoulou, V. et al. Itaconate links inhibition of succinate dehydrogenase with macrophage metabolic remodeling and regulation of inflammation. Cell. Metab. 24, 158–166 (2016).
Luan, H. H. & Medzhitov, R. Food fight: role of itaconate and other metabolites in antimicrobial defense. Cell. Metab. 24, 379–387 (2016).
Nemeth, B. et al. Abolition of mitochondrial substrate-level phosphorylation by itaconic acid produced by LPS-induced Irg1 expression in cells of murine macrophage lineage. FASEB J.: Off. Publ. Fed. Am. Soc. Exp. Biol. 30, 286–300 (2016).
Booth, A. N., Taylor, J., Wilson, R. H. & Deeds, F. The inhibitory effects of itaconic acid in vitro and in vivo. J. Biol. Chem. 195, 697–702 (1952).
Cordes, T. et al. Immunoresponsive Gene 1 and itaconate inhibit succinate dehydrogenase to modulate intracellular succinate levels. J. Biol. Chem. 291, 14274–14284 (2016).
Ganta, V. C. et al. A MicroRNA93-IRF9-IRG1-Itaconic Acid Pathway Modulates M2-like-Macrophage Polarization to Revascularize Ischemic Muscle. Circulation 135, 2403–2425 (2017).
Tallam, A. et al. Gene Regulatory network inference of immunoresponsive gene 1 (IRG1) identifies interferon regulatory factor 1 (IRF1) as its transcriptional regulator in mammalian macrophages. PLoS ONE 11, e0149050 (2016).
Van den Bossche, J., O'Neill, L. A. & Menon, D. Macrophage immunometabolism: where are we (Going)? Trends. Immunol. 38, 395–406 (2017).
Izquierdo, E. et al. Reshaping of human macrophage polarization through modulation of glucose catabolic pathways. J. Immunol. 195, 2442–2451 (2015).
Viguerie, N. et al. Determinants of human adipose tissue gene expression: impact of diet, sex, metabolic status, and cis genetic regulation. PLoS Genet. 8, e1002959 (2012).
Capel, F. et al. Macrophages and adipocytes in human obesity: adipose tissue gene expression and insulin sensitivity during calorie restriction and weight stabilization. Diabetes 58, 1558–1567 (2009).
Behmoaras, J. et al. Genetic loci modulate macrophage activity and glomerular damage in experimental glomerulonephritis. J. Am. Soc. Nephrol. 21, 1136–1144 (2010).
Chen, T. D. et al. Identification of ceruloplasmin as a gene that affects susceptibility to glomerulonephritis through macrophage function. Genetics 206, 1139–1151 (2017).
Rotival, M. et al. Integrating phosphoproteome and transcriptome reveals new determinants of macrophage multinucleation. Mol. Cell. Proteom. 14, 484–498 (2015).
Li, J., Hsu, H. C. & Mountz, J. D. Managing macrophages in rheumatoid arthritis by reform or removal. Curr. Rheumatol. Rep. 14, 445–454 (2012).
Neishabouri, S. H., Hutson, S. M. & Davoodi, J. Chronic activation of mTOR complex 1 by branched chain amino acids and organ hypertrophy. Amino Acids 47, 1167–1182 (2015).
She, P. et al. Disruption of BCATm in mice leads to increased energy expenditure associated with the activation of a futile protein turnover cycle. Cell Metab. 6, 181–194 (2007).
Sweatt, A. J., Garcia-Espinosa, M. A., Wallin, R. & Hutson, S. M. Branched-chain amino acids and neurotransmitter metabolism: expression of cytosolic branched-chain aminotransferase (BCATc) in the cerebellum and hippocampus. J. Comp. Neurol. 477, 360–370 (2004).
Bhan, A. K., Schneeberger, E. E., Collins, A. B. & McCluskey, R. T. Evidence for a pathogenic role of a cell-mediated immune mechanism in experimental glomerulonephritis. J. Exp. Med. 148, 246–260 (1978).
This work was supported by the Medical Research Council (MR/M004716/1 and MR/N01121X/1 to J.B.) and by Kidney Research UK (RP9/2013 to J.B.). D.C. is supported by the Institute Pasteur, Fondazione Cenci Bolognetti. C.M. is supported by the British Hearth Foundation Fellowship (FS/12/3829640). We are grateful to Dr Susan Hutson (Virginia Tech) for the generous gift of BCAT1 antibody, which was used to detect BCAT1 protein in human macrophages by E.A.A.
A.E.P. is an employee of Ergon Pharmaceuticals Ltd. The remaining authors declare no competing financial interests.
About this article
Cite this article
Papathanassiu, A., Ko, JH., Imprialou, M. et al. BCAT1 controls metabolic reprogramming in activated human macrophages and is associated with inflammatory diseases. Nat Commun 8, 16040 (2017). https://doi.org/10.1038/ncomms16040
Nature Reviews Rheumatology (2022)
Nature Microbiology (2022)
Protein & Cell (2022)
BCAT1 decreases the sensitivity of cancer cells to cisplatin by regulating mTOR-mediated autophagy via branched-chain amino acid metabolism
Cell Death & Disease (2021)
An overview of branched-chain amino acid aminotransferases: functional differences between mitochondrial and cytosolic isozymes in yeast and human
Applied Microbiology and Biotechnology (2021)