The tremendous reservoir of soil organic carbon (SOC) in wetlands is being threatened by water-table decline (WTD) globally. However, the SOC response to WTD remains highly uncertain. Here we examine the under-investigated role of iron (Fe) in mediating soil enzyme activity and lignin stabilization in a mesocosm WTD experiment in an alpine wetland. In contrast to the classic ‘enzyme latch’ theory, phenol oxidative activity is mainly controlled by ferrous iron [Fe(II)] and declines with WTD, leading to an accumulation of dissolvable aromatics and a reduced activity of hydrolytic enzyme. Furthermore, using dithionite to remove Fe oxides, we observe a significant increase of Fe-protected lignin phenols in the air-exposed soils. Fe oxidation hence acts as an ‘iron gate’ against the ‘enzyme latch’ in regulating wetland SOC dynamics under oxygen exposure. This newly recognized mechanism may be key to predicting wetland soil carbon storage with intensified WTD in a changing climate.
Wetlands, covering 5–8% of the Earth’s land surface, store up to 535 Gt of carbon below ground, representing ∼30% of global terrestrial carbon pool1,2. This massive carbon stock results from outweighed primary production relative to hampered decomposition under water-logged conditions3. However, under land-use and climate changes1,4, about half of the global wetland area (2.7–6.4 million km2) has been lost due to water-table decline (WTD)1. Improved drainage during WTD or drought may turn wetlands from carbon sinks to sources through increasing decomposition, causing major concern for carbon sequestration and climate mitigation procedures5,6.
The long-standing view on the response of wetland soil organic carbon (SOC) to WTD focuses on the positive feedback between oxygen-enhanced extracellular enzyme activity and SOC decomposition5,7,8. It is shown that increased oxygen availability may substantially promote the activity of phenol oxidase8,9. As elevated phenol oxidative activity eliminates phenol compounds that are toxic to hydrolytic enzymes, oxygen exposure during wetland WTD further stimulates the degradation of cellulosic compounds via breaking the ‘enzyme latch’8,10,11. However, positive, neutral and negative effects of WTD on SOC decomposition have been reported5,12,13,14,15, suggesting that other competing or confounding mechanisms may govern SOC dynamics during wetland WTD.
Redox-induced transformation of iron (Fe) is a key biogeochemical process during wetland WTD that involves the mobilization and stabilization of carbon, nitrogen, sulfur and phosphorus16. Several studies have observed that the presence of ferrous iron [Fe(II)] in hypoxic peatland soils may enhance phenol oxidative activity17,18,19. Recently, Hall et al.20 also demonstrated that the activities of hydrolytic enzymes increased with Fe(II) under anaerobic conditions, in contrast to the ‘enzyme latch’ hypothesis. Therefore, as soluble Fe(II) is oxidized to less soluble ferric iron [Fe(III)] during wetland WTD, the decline of Fe(II) may be accompanied by reduced activities of oxidative and hydrolytic enzymes, thereby counteracting the ‘enzyme latch’ and enhancing SOC preservation. Furthermore, organic matter (OM) may sorb or co-precipitate with the newly-formed reactive Fe(III) (hydro)oxides (especially poorly crystalline Fe oxides), forming Fe–OM complexes that are chemically more stable21,22. Such Fe–OM associations are considered to play an important role in carbon preservation21,23,24 and are estimated to stabilize ca. 21.5% of organic carbon in sediments25 and ca. 37.8% of SOC in US forest soils26. In particular, aromatics and phenolics that accumulate in wetlands are shown to exhibit a strong affinity to Fe oxides at the redox interface27,28, potentially protected from mineralization during oxygen exposure29.
As the second most abundant component of terrestrial plants, lignin is made up of phenolic units whose decomposition is optimized in aerobic environments30,31. In anaerobic wetlands, lignin-derived phenolic moieties are relatively concentrated and exert an important control on enzyme activity and SOC dynamics31. During redox fluctuations in wetlands, lignin and Fe may undergo strong association-remobilization processes. For instance, a recent incubation study employing 13C-labelled lignin demonstrated that oxidation of Fe(II) in a humid tropical soil specifically suppressed lignin mineralization relative to bulk SOC likely due to the association between lignin and Fe oxides29. However, Fe–lignin interaction during wetland WTD remains under-investigated. A major impediment to progress is that Fe-bound lignin moieties cannot be analysed by conventional method based on copper oxide (CuO) oxidation32, which breaks ether linkages in the macromolecular lignin and generates identifiable phenol monomers referred to as lignin phenols33. As demonstrated by Hernes et al.32, sorption to Fe oxides and minerals may ‘hide’ lignin and significantly reduce the yield of lignin phenols upon CuO oxidation. Therefore, an improved method is needed to quantify Fe-bound lignin phenols in the soil matrix. Recently, Shields et al.34 observed altered yields of lignin phenols upon CuO oxidation after removing reactive Fe with dithionite from Mississippi delta sediments due to the release of Fe-bound lignin. While this method has not been further validated or applied to other systems so far, it may potentially provide new insights on Fe-lignin interaction during redox changes in wetlands.
In this study, we employ a novel approach to examine Fe–SOC interaction in response to WTD-induced redox changes in a mesocosm water-table manipulation experiment on the Qinghai-Tibetan Plateau. The experiment consists of duplicated control (with submerged soils) and WTD (−20 cm relative to control) treatments in an alpine wetland dominated by Carex pamirensis (cf. Wang et al.35,36 for details of the experimental design). After 27 months of WTD treatment, extracellular enzyme activity, SOC and lignin phenols are examined together with different Fe species in the air-exposed (0–20 cm) relative to the submerged (30–40 cm) soil layers. In particular, similar to Shields et al.34, we use dithionite to remove crystalline and amorphous Fe oxides25 that may associate with SOC and quantify Fe-bound lignin phenols that are subsequently exposed and amenable to CuO oxidation. This method is tested using a series of supplementary analyses and applied to the wetland soils. It is observed that up to an average of 40% of lignin phenols are ‘protected’ by reactive Fe in the subsoils and not detected by the conventional CuO oxidation method. More importantly, in line with previous findings20,29, wetland WTD not only decreases phenol oxidative activity and β-1,4-glucosidase (β-glucosidase) activity but also increases Fe-bound lignin phenols in the air-exposed soils due to Fe(II) oxidation. We hence propose that redox-induced Fe transformation may act as an ‘iron gate’ against the ‘enzyme latch’ in regulating SOC dynamics under oxygen exposure during wetland WTD. This mechanism may contribute to SOC preservation in the long term and be key to understand the contrasting responses of wetland carbon storage to WTD or drought under global changes.
Fe species and enzyme activity
In the studied alpine wetland (Fig. 1), Fe(II) dominated over Fe(III), accounting for >72% of total Fe in the soil (Fig. 2a,b). Compared to the control, WTD treatment significantly decreased Fe(II) content in the air-exposed soil layers (0–20 cm; P<0.05), reflecting oxidative removal of Fe(II) under oxygen exposure. Interestingly, both phenol oxidative and β-glucosidase activities decreased with WTD in these layers (0–20 cm; Fig. 2c,d), accompanied by a significant decrease of water-extractable organic carbon (WEOC), an increase of its aromaticity assessed by specific ultraviolet absorbance at 254 nm (SUVA254) and a decrease of soil pH (P<0.05; Fig. 2e–g).
Different forms of extractable Fe
Different forms of Fe oxides were examined to reveal the distribution of reactive Fe in the soil. Dithionite-extractable Fe (Fed) had similar concentrations to oxalate-extractable Fe (Feo) across all depths (Supplementary Fig. 1a,b), suggesting the dominance of amorphous (rather than crystalline) Fe oxides in the wetland soils. By comparison, pyrophosphate-extractable Fe (Fep) had much lower concentrations (Supplementary Fig. 1c). None of these Fe contents showed any significant response to the WTD treatment due to high sample variabilities. The ratio of Fep:Fed, an indicator for Fe complexation degree, increased in the deeper soils (Supplementary Fig. 1d), suggesting enhanced Fe–OM complexation at depth.
Fe-bound SOC and lignin phenols
SOC and lignin phenol contents of the fine soil fraction (<53 μm) were analysed before and after removal of crystalline and amorphous Fe oxides using the citrate–bicarbonate–dithionite (CBD) method25,34,37 to examine OM association with Fe oxides during WTD. Bulk SOC was overall not affected by WTD in the air-exposed or submerged soils (Fig. 2h). The dithionite treatment released significant amount of Fe-bound SOC, accounting for 5.4–11.8% of the original SOC content (Fig. 2i). The molar ratio of Fe-bound SOC to dithionite-extractable Fe (Fed) ranged 7.3–16.8 (Fig. 2j) across the soil profile, suggesting that co-precipitation or complexation rather than sorption (usually with Fe-bound SOC:Fed<1) dominated Fe–OM interactions in the studied wetland38,39. Notably, Fe-bound SOC was significantly higher in the submerged (30–40 cm) soil rather than in the air-exposed soils under the WTD treatment relative to the control (P<0.05), accompanied by a significant decrease of lignin phenols at the same depth (P<0.05; Fig. 2k).
Consistent with the ‘hidden lignin’ framework demonstrated by Hernes et al.32, the dithionite treatment exposed Fe-bound lignin phenols that were otherwise not detected by the CuO oxidation method, thus producing mostly positive values of Fe-bound lignin phenols (Fig. 2l). In particular, Fe-bound lignin phenols accounted for up to an average of 40% of lignin phenols in the original subsoil (30–40 cm; untreated with dithionite), representing a significant pool of lignin that has been underestimated in the sedimentary settings. Remarkably, Fe-bound lignin phenols were significantly higher in the air-exposed soil layers under WTD compared to the control (P<0.05; Fig. 2l), indicating that oxygen exposure induced stabilization of lignin in the Fe-bound form.
OM sources in the subsoil
To gain further information on the OM sources in the subsoil (30–40 cm) that witnessed a significant increase of Fe-bound SOC under WTD, we conducted a supplementary analysis of neutral sugars (that is, non-cellulosic polysaccharides), which may differentiate between microbial versus plant sources40,41. Neutral sugars amounted to 198±49 and 481±99 mg g−1 SOC in the WTD and control soils, respectively, representing a substantial and much larger fraction of bulk SOC relative to lignin phenols. The SOC-normalized concentration of neutral sugars decreased by ca. 50% under WTD compared to the control (P<0.05; Fig. 3a), echoing the response of lignin phenols. Notably, the ratio of microbial versus plant-derived sugars [(galactose+mannose)/(arabinose+xylose)]40 was significantly lower under WTD than the control (P<0.05; Fig. 3b), suggesting a preferential retention of plant-derived sugars in the WTD subsoils.
In our mesocosm experiment, the WTD-inhibited phenol oxidative activity stands in contrast with the hypothesized opening of the ‘enzyme latch’ upon oxygen exposure7,42,43,44 but bears resemblance to a previous observation, where phenol oxidative activity increased in tandem with elevated Fe(II) and dissolved organic carbon during flooding of a paddy soil45. It is noteworthy that phenol oxidative and β-glucosidase activities are positively correlated with Fe(II) concentrations in our experiment (P<0.05; Fig. 4a), which is consistent with several previous reports17,18,19,20 and may be attributed to the following mechanisms. First, it is known that Fe(II) may enhance phenol oxidative activity by catalysing the production of hydroxyl radicals17,18,19. Hence, as Fe(II) concentration sharply dropped in the soils exposed to oxygen, phenol oxidative activity also declined. Second, similar to many other studies19,46,47, WTD decreased soil pH in the air-exposed soils (Fig. 2g) through proton release during the hydrolysis of Fe(III) produced from Fe(II) oxidation19. As phenol oxidative activity increases with pH in our experiment (P<0.05; Fig. 4b) as well as at the ecosystem level18, a pH decline during WTD may contribute to the inhibited phenol oxidative activity48. Acidification is also associated with the decreased WEOC under WTD49,50, since most soil OM has a high pKa51, hence leading to a negative correlation between pH and WEOC in our wetland soils (P<0.01). As WEOC represents the most bioavailable fraction of SOC52, its decline may further constrain the production and activity of extracellular enzymes due to low substrate availability for soil microbes53,54. The reduced phenol oxidative activity was associated with the increased aromaticity of WEOC in the WTD-treated soils (P<0.05; Fig. 4b), contributing to the decline of β-glucosidase activity due to phenols’ toxicity to hydrolytic enzymes7,55.
To separate the effect of Fe(II) and pH on phenol oxidative activity, a partial correlation test was conducted. Phenol oxidative activity and Fe(II) are significantly corrrelated after the effect of pH is accounted for (P<0.01). In contrast, pH has no effect on phenol oxidative activity after the effect of Fe(II) content is accounted for (P=0.49). This result suggests that Fe(II) is the main determinant of phenol oxidative activity in our study, counteracting the positive impact of oxygen exposure during WTD. WTD may therefore have varied effects on phenol oxidative activity in wetland soils, not necessarily leading to an opening of the ‘enzyme latch’.
So where does Fe(II) play an important role in mediating the response of phenol oxidative activity to water-table variations? To answer this question, we surveyed the current literature to compare vegetation and soil characteristics of various studies that reported extracellular enzyme (mainly phenol oxidative) activities in response to WTD or drought (Supplementary Table 1). It is found that studies reporting increasing phenol oxidative activities with WTD or drought were all based on Sphagnum-dominated peat5,8,10,56. By comparison, WTD- or drying-induced decrease of phenol oxidative or hydrolytic enzyme activities was observed in soils dominated by non-Sphagnum (vascular) species (such as sedge, rice, shrub and trees)19,20,45,47,48,57. As Sphagnum has a much lower lignin content in their biomass as compared with vascular plants (Supplementary Fig. 2), it is reasonable to postulate that there is a lower lignin phenol content in the Sphagnum-dominated peat per unit of SOC relative to vascular plant-dominated soils. Varied lignin phenol contents may influence the speciation and concentration of Fe through sorption and complexation processes27,58 and thereby regulate the balance between O2 and Fe(II) effects on phenol oxidative activities. Alternatively, the majority (five out of seven) of the studies reporting a negative enzyme response to increasing O2 were based on mineral soils or organic horizons overlaying mineral soils (as is this study). The abundance or availability of Fe(II) may also play a key role in the response of extracellular enzymes to changing redox conditions. Hence, we postulate that the effect of ‘enzyme latch’ varies depending on both OM composition and soil type. The observed Fe(II) control on phenol oxidative activity is likely to be more important in mineral-rich and/or vascular plant-dominated wetland environments.
Fe did not only mediate soil carbon’s response to WTD through altering soil enzyme activities but also through changing its association with lignin. The observed increase of Fe-bound lignin phenols in the air-exposed soil layers echoes lignin stabilization with the oxidation of added Fe(II) into a moist tropical soil29. Since none of the extractable Fe increased under WTD (Supplementary Fig. 1), the accumulation of Fe-bound lignin phenols was likely driven by enhanced complexation (or co-precipitation) rather than sorption to Fe, consistent with the high Fe-bound SOC:Fed ratios. As aromatic and phenolic compounds have a strong affinity for hydrous oxides23,28, an increased aromaticity in the WEOC (Fig. 2e) may have promoted or reflected the stronger association between lignin and Fe under WTD. This hypothesis is confirmed by the positive correlation between Fe-bound lignin phenols and the aromaticity (SUVA254) of WEOC across the entire soil profile (P<0.05; Fig. 4c). Alternatively, Chen et al.58 showed that a pH decline may increase the amount of OM adsorbed to ferrihydrite. Hence, pH decrease in the air-exposed soils (Fig. 2g) may have also led to increased lignin complexation with Fe, reflected by the significant, negative correlation between Fe-bound lignin phenols and soil pH in our soils (P<0.01; Fig. 4c). Overall, our results provide clear evidence for the under-recognized role of Fe in stabilizing phenolic SOC during wetland WTD.
Last but not least, the observed increase of Fe-bound SOC at 30–40 cm was quite unexpected given that soils at this depth were continuously submerged in water in both treatments and that none of the extractable Fe oxides (Supplementary Fig. 1) or enzyme activities varied between the control and WTD treatments. However, root biomass significantly increased at 20–30 cm under WTD while remaining similar at the other depths35. We therefore postulate that a higher amount of root exudates migrated downward to the deeper soil in a mobile colloidal form associated with Fe59, thereby increasing Fe-bound SOC at 30–40 cm under WTD. Unfortunately, due to the high solubility of root exudates (such as small organic acids and sugars and so on)60, they are prone to loss during the CBD treatment, and hence we do not know of a method to analyse Fe-bound sugars (or organic acids) to confirm their accumulation yet. Nonetheless, the preferential retention of plant-derived sugars in the soil (Fig. 3b) somewhat agrees with our postulated increase of root-derived OM at 30–40 cm under WTD. Because Fe-bound SOC had a lower extractability of lignin phenols32 (and presumably sugars as well), its increase diluted lignin phenols and, to a higher extent, neutral sugars in the bulk soil at this depth. It is also possible that a higher amount of root-derived sugars were associated with Fe61 in the WTD subsoil, reducing their extractability and contributing to the lower concentration of neutral sugars in the hydrolysable fraction.
In summary, our innovative approach employing dithionite treatment coupled with lignin phenol analysis enabled the detection of Fe-bound lignin phenols in the wetland soil matrix, which accounted for up to an average of 40% of lignin phenols in the original subsoil. In sedimentary settings with abundant aromatic structures, Fe-bound lignin may represent a significantly underestimated pool of terrestrial carbon. Moreover, our detailed investigation on the alteration of Fe, enzyme and lignin moieties during WTD showed that oxygen exposure may not necessarily increase wetland soil carbon loss through opening the ‘enzyme latch’. In the Carex-dominated alpine wetland underlain by Fe-rich soils, Fe exerted a strong control on SOC dynamics by inhibiting phenol oxidative activity and promoting Fe-lignin association through Fe(II) oxidation and pH decline during WTD (the ‘iron gate’ mechanism; Fig. 5). As Fe-bound SOC moieties are considered to be more stable chemically21,22, wetland WTD may have a differential effect on the stable soil carbon pool in the longer term. Hence, redox-induced transformation of Fe plays an important and counteracting role with oxygen availability in regulating enzyme activity and SOC dynamics under wetland WTD. This newly recognized mechanism may be a key to understanding the contrasting responses of wetland carbon storage to WTD or drought in different regions, but its operating conditions and magnitude remain unclear. For instance, it is unclear if the ‘iron gate’ mechanism may outcompete ‘enzyme latch’ in the long term when reactive Fe becomes depleted or when Fe transformation slows down. Coupled Fe–SOC investigations are also needed in a wider range of wetland types to ascertain the prevalence of ‘iron gate’ in mineral-rich as well as vascular plant-dominated wetlands in order to fully assess its implications for global carbon budgets and wetland biogeochemistry in a changing climate.
Study site and WTD experiment
The study area is located at the Luanhaizi wetland, an alpine wetland on the northeastern edge of Qinghai-Tibet Plateau (37°35′ N, 101°20′ E, 3,250 m above sea level), with a mean annual temperature of −1.1 °C and a mean annual precipitation of 490 mm (ref. 62). The wetland is dominated by peaty soil (silty clay loam of Mat-Cryic Cambisol62) ranging from 0.2 to 2.0 m in depth. The average SOC and N content in the upper 0–30 cm is 21.6 and 1.2% (ref. 35), respectively. The dominating vegetation is Carex pamirensis, with several other species including Carex atrofusca, Hippuris vulgaris, Triglochin palustre and Heleocharis spp.
For the mesocosm experiment, 20 bottomless metal tanks with antirust paint (60 cm × 60 cm × 65 cm) were randomly embedded into the centre region of the Luanhaizi wetland in October 2010. In early November, when soils were frozen, the tanks were excavated with the bottoms sealed by painted metal plates and transported to the Haibei Alpine Grassland Ecosystem Research Station nearby. These tanks were surrounded by plastic foams to decrease energy exchange with the atmosphere. A transparent plastic canopy was built to prevent the influence of precipitation. One slim polyvinylpyrrolidone tube was setup at the corner of each box for water-table observation (Fig. 1; cf. Wang et al.36). The experiment started in mid-June of 2011, consisting of control (with submerged soils, n=4) and WTD treatments (n=4). Both treatments were automatically watered via micropumps (PULANDI 1205 Diaphragm Pump, Pulandi Machine Equipment Co., Shijiazhuang, China) connected to manostat systems using natural wetland water, which contained very low amounts of dissolved organic carbon and Fe such that their total inputs were negligible during the entire experiment (total dissolved organic carbon: ∼0.52–1.61 μg g−1 soil; total dissolved Fe: <0.008 μg g−1 soil). Water table was kept at 1.29±0.42 cm above and 23±0.83 cm below soil surfaces in the control and WTD treatment from June to September, respectively36. Soil surface was frozen from November to March/April and hence not monitored. The volume of soil column was not observed to change during the experiment.
Soil sampling and preparation
In September 2013, multiple soil cores were sampled using soil corer (5 cm in diameter) from the centre of each treatment box and sliced into different depth sections. Four depths were chosen for subsequent analyses (0–4, 4–10, 10–20 and 30–40 cm) and combined accordingly. The first three layers (0–20 cm) were exposed to the air in the WTD treatment and submerged in water in the control. The fourth layer was continuously submerged in water in both treatments. Fresh soil samples were transported to the laboratory at 4 °C. An aliquot was used to determine enzyme activity and Fe species within 1 week (analytical schemes in Supplementary Fig. 3). Soil pH was measured by a pH metre with a soil:water ratio of 1:5 (w:w). The remaining soil was freeze-dried and sieved into three fractions: 0–53, 53–150 and 150–2,000 μm. The coarse fractions (>53 μm) were dominated by root debris. We hence focused on the finest fraction (0–53 μm) to explore SOC and lignin dynamics, which accounted for 40–60% of bulk soil mass (Supplementary Fig. 4). SOC was determined by Vario EL III (Elementar Analysensysteme, Hanau, Germany) elemental analyzer after fumigation with concentrated HCl63.
Soil assay of extracellular enzyme activity
Potential phenol oxidative activity (phenol oxidase: EC 126.96.36.199) and β-glucosidase (EC 188.8.131.52) activity were measured according to Saiya-Cork et al.64. Specifically, ∼1 g of fresh bulk soil was homogenized with 125 ml of tris buffer (50 mM, pH 7.8) for 2.5 min with a magnetic stirrer. The resulting suspension (200 μl) was dispensed into 96-well microplates (eight replicate wells per sample per assay) while 50 μl of MUB-β-glucoside (200 μM) or L-3,4-dihydroxyphenylalanine (L-DOPA; 5 mM) solutions were added to each sample well, respectively. The microplates were incubated in the dark at 20 °C for 16 and 4 h for phenol oxidative and β-1,4-glucosidase activities, respectively. Absorbance was measured using Multi-Mode Microplate Reader (synergy Mx, BioTek Instruments Inc., USA) at 450 nm, while fluorescence was measured with excitation at 365 nm and emission at 450 nm. Enzyme activity was expressed in units of nmol h−1 g−1 dry soil or μmol h−1 g−1 dry soil.
An additional experiment utilizing surface wetland soils showed that cumulative L-DOPA oxidative products (calculated using light absorbance values) increased linearly with incubation time within 20 h (P<0.05; Supplementary Fig. 5). Hence, an incubation time of 16 h is reasonable for the assay of phenol oxidative activity here. Sorption of L-DOPA substrate onto mineral soils is known to bias phenol oxidative measurements65, rendering the absolute magnitude of phenol oxidative potentials not directly comparable among different soils. However, this mineral effect is not likely to be important in this study examining soil profiles with similar mineralogy and SOC content.
Water-extractable OM analyses
WEOC was extracted by shaking 2.0 g of dried soil (<53 μm) in 35 ml of Milli-Q water on a shaker for 12 h. The suspension was filtered through pre-combusted 0.7-μm Whatman GF/F filters. An aliquot of the filtrate was acidified to pH <2 with HCl for WEOC measurement on multi N/C 3100 (Analytikjena, Germany) total organic carbon analyzer. Ultraviolet absorbance was also measured for acidified water extracts between 200 and 800 nm on an ultraviolet and visible spectrometer (Shimadzu UV-2550). Specific ultraviolet absorbance at 254 nm (SUVA254) indicating aromaticity66 was calculated as the decadal absorption coefficient divided by WEOC.
Analysis of Fe and Fe-bound SOC
Soil Fe(II) and Fe(III) contents were analysed using the ferrozine-ultraviolet absorbance method67. Specifically, ∼0.2 g of fresh bulk soil was extracted by 5 ml of 0.5-M HCl overnight. Fe(II) was measured by absorbance at 562 nm on a UV-Vis spectrometer (Shimadzu UV-2550) after mixing with 5-mM ferrozine solution. Total Fe was first reduced using hydroxylamine hydrochloride (2%) and then measured as Fe(II) as above. Fe(III) was calculated by subtracting Fe(II) from total Fe and expressed in mg g−1 soil. Fep and Feo were extracted from dried soils (<53 μm), representing OM-complexed and poorly-crystalline Fe oxides, respectively68. Fep was extracted in 0.1 M sodium polyphosphate decahydrate at a soil:solution ratio of 1:100 (w:v) according to Mikutta et al.69. Feo was extracted with 0.2 M ammonium oxalate at pH 3, with a soil:solution ratio of 1:50 (w:v). Fed was extracted using modified CBD method25,37. The CBD supernatant was filtered through pre-combusted 0.7-μm GF/F filters, acidified to pH 2 to maintain Fe in the solution and kept in dark at 4 °C for 2 days to allow the oxidation of dithionite. Brownish precipitates were observed at this step (referred to as ‘CBD precipitates’) likely due to coagulation of OM with inorganics and was recovered using centrifugation. Fep, Feo and Fed contents in the solution were determined on an inductively coupled plasma-optical emission spectrometer (ICP-OES; iCAP 6300, Thermo Scientific, USA). Soils from 0–4 cm were not included for the CBD treatment due to limited sample size.
Fe-bound SOC was determined in tandem with the CBD treatment according to Lalonde et al.25. Briefly, dried soils (<53 μm) were extracted with sodium chloride (NaCl) instead of CBD at an equivalent ionic strength as control experiments. Soil residues were rinsed with 10 ml of NaCl solution (1 M) three times, dried and HCl-fumigated before organic carbon measurement. Fe-bound SOC (in percentage) was determined as:
where OCNaCl and OCCBD are the organic carbon content of NaCl- and CBD-treated soil residues, respectively. The molar ratio of Fe-bound SOC:Fed was used as an indicator of Fe–OM interaction type25,58, which is <1.0 for sorption and >6 for co-precipitation38,39.
Lignin phenols in the freeze-dried water-extracted soils, CBD-OM precipitates and CBD-treated residues of soils (<53 μm) were analysed using CuO oxidation method70,71. Briefly, dried soils or OM were mixed with 1 g CuO, 100 mg ammonium iron (II) sulfate hexahydrate and 15 ml of 2 M NaOH in teflon-lined bombs at 150 °C for 2.5 h. Lignin oxidation products (LOPs) were recovered from the aqueous phase with ethyl acetate after acidification with HCl (pH <2). Ethyl vanillin (3-ethoxy-4-hydroxy-benzaldehyde) and trans-cinnamic acid (3-phenyl-2-propenoic acid) were used as the recovery and internal standards, respectively. Aliquots of the LOPs were converted to trimethylsilyl derivatives and quantified on a gas chromatography-mass spectrometry (GC-MS; Thermo Fisher Scientific, USA) equipped with a DB-5 MS capillary column (30 m × 0.25 mm × 0.25 μm). Eight characteristic lignin phenols were summarized to represent SOC-normalized lignin phenol content (Λ) in the units of mg g−1 SOC, including vanillyl (vanillin, acetovanillone, vanillic acid), syringyl (syringaldehyde, acetosyringone, syringic acid) and cinnamyl (p-coumaric acid, ferulic acid) phenols.
Assuming that CBD treatment exposed Fe-bound lignin that was originally not amenable to CuO oxidation32,34, we estimated Fe-bound lignin phenols (in percentage) as:
where Λr, Λp and Λsoil represent SOC-normalized lignin phenol concentrations in the CBD-treated soil residues, CBD-OM precipitates and water-extracted soil, respectively.
Preliminary experiment of CBD effect on lignin phenol yield
A preliminary experiment was conducted to test whether additional lignin phenols were produced from non-Fe-bound OM following CBD treatment. Towards this end, water-soluble (that is, non-Fe-bound) plant OM was extracted from oak leaves by shaking in MilliQ water overnight, filtered through 0.45 μm and diluted to a dissolved organic carbon concentration of ∼100 mg C l−1. Two aliquots of the water extracts (40 ml each) were subject to CBD treatment using the same protocol as soil samples, except that dithionite was not added to one of the aliquots serving as a control. Dissolved OM in the CBD-treated solutions was recovered using C18 solid phase extraction cartridges and analysed by CuO oxidation method according to Louchouarn et al.72 and Spencer et al.73. As shown in Supplementary Fig. 6, both vanillyl and syringyl phenols had similar yield from the CBD-treated and control plant extracts, suggesting that CBD treatment did not cause secondary artifacts or affect the CuO oxidation efficiency for these two groups of lignin phenols. However, cinnamyl phenols decreased by ∼50% in the CBD-treated extract relative to the control, suggesting that cinnamyl phenols were subject to loss during CBD treatment and should be excluded in the calculation of Fe-bound lignin phenols. The cause for the low recovery of cinnamyl phenols remains unclear, possibly related to a lower solid phase extraction extract efficiency for the more soluble cinnamyl phenols from the aqueous phase and/or potential Fenton-like reactions with the more degradable cinnamyl phenols74,75. Regardless of the cause, our preliminary results suggest that CBD treatment is unlikely to increase the yield of CuO oxidation through producing secondary lignin phenol structures from non-Fe-bound OM. This result is echoed by the decreased yield of cinnamyl phenols but increased yields of vanillyl and syringyl phenols from the control soils after CBD treatment. Hence, we only used vanillyl and syringyl phenols for the calculation in equation (2). We also measured lignin phenols in the water extract and CBD solution of soils from different depths, which accounted for <0.4% and <5% of total lignin phenols in the original soil (Supplementary Figs 7 and 8), respectively. Soil water extract and CBD solution were hence not included in the analysis. However, it should be noted that potential loss of soluble lignin during the CBD treatment may lead to negative values of Fe-bound lignin phenols.
Analysis of neutral sugars
Seven neutral sugars (glucose, galactose, arabinose, xylose, mannose, rhamnose and ribose) were extracted according to Spielvogel et al.40. Briefly, about 0.4 g of dried soil (<53 μm) was hydrolysed by 4 M trifluoroacetic acid at 105 °C for 4 h. Xylitol was added as a recovery standard. Neutral sugar monomers were converted to alditols and subsequently acetylated with myo-inositol added as an internal standard. Sugar monomers were quantified on a GC-MS as described previously.
Statistical analyses were performed using SPSS 20. Differences in SOC, enzyme activity, Fe species, lignin phenol contents and neutral sugars between control and WTD treatments were examined by two-way ANOVA with depth as a fixed effect for the air-exposed soil layers (0–20 cm) and by t-test for the submerged soil layer (30–40 cm). Relationships between different variables were tested by Pearson correlation tests. A partial linear correlation test was conducted to examine the effect of Fe(II) content or soil pH on phenol oxidative activity with either variable being controlled. Correlations and differences were considered to be significant at a level of P<0.05.
The data that support the findings of this study are available from the corresponding author upon request.
How to cite this article: Wang, Y. et al. Iron-mediated soil carbon response to water-table decline in an alpine wetland. Nat. Commun. 8, 15972 doi: 10.1038/ncomms15972 (2017).
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Zedler, J. B. & Kercher, S. Wetland resources: status, trends, ecosystem services, and restorability. Annu. Rev. Env. Resour. 30, 39–74 (2005).
Mitsch, W. J. et al. Wetlands, carbon, and climate change. Landscape Ecol. 28, 583–597 (2012).
Dise, N. B. in The Wetlands Handbook eds Maltby E., Barker T. 249–265Wiley-Blackwell (2009).
Dise, N. B. Peatland response to global change. Science 326, 810–811 (2009).
Fenner, N. & Freeman, C. Drought-induced carbon loss in peatlands. Nat. Geosci. 4, 895–900 (2011).
Malone, S. L., Starr, G., Staudhammer, C. L. & Ryan, M. G. Effects of simulated drought on the carbon balance of Everglades short-hydroperiod marsh. Glob. Change Biol. 19, 2511–2523 (2013).
Freeman, C., Ostle, N. & Kang, H. An enzymic ‘latch’ on a global carbon store—a shortage of oxygen locks up carbon in peatlands by restraining a single enzyme. Nature 409, 149–149 (2001).
Freeman, C., Ostle, N. J., Fenner, N. & Kang, H. A regulatory role for phenol oxidase during decomposition in peatlands. Soil Biol. Biochem. 36, 1663–1667 (2004).
Zibilske, L. M. & Bradford, J. M. Oxygen effects on carbon, polyphenols, and nitrogen mineralization potential in soil. Soil Sci. Soc. Am. J. 71, 133–139 (2007).
Freeman, C. et al. Microbial activity and enzymic decomposition processes following peatland water table drawdown. Plant Soil 180, 121–127 (1996).
Ise, T., Dunn, A. L., Wofsy, S. C. & Moorcroft, P. R. High sensitivity of peat decomposition to climate change through water-table feedback. Nat. Geosci. 1, 763–766 (2008).
Blodau, C., Basiliko, N. & Moore, T. R. Carbon turnover in peatland mesocosms exposed to different water table levels. Biogeochemistry 67, 331–351 (2004).
Carter, M. S. et al. Synthesizing greenhouse gas fluxes across nine European peatlands and shrublands—responses to climatic and environmental changes. Biogeosciences 9, 3739–3755 (2012).
Wang, H., Richardson, C. J. & Ho, M. Dual controls on carbon loss during drought in peatlands. Nat. Clim. Change 5, 584–587 (2015).
Muhr, J., Höhle, J., Otieno, D. O. & Borken, W. Manipulative lowering of the water table during summer does not affect CO2 emissions and uptake in a fen in Germany. Ecol. Appl. 21, 391–401 (2011).
Li, Y., Yu, S., Strong, J. & Wang, H. Are the biogeochemical cycles of carbon, nitrogen, sulfur, and phosphorus driven by the ‘FeIII–FeII redox wheel’ in dynamic redox environments? J. Soils Sediments 12, 683–693 (2012).
van Bodegom, P. M., Broekman, R., Van Dijk, J., Bakker, C. & Aerts, R. Ferrous iron stimulates phenol oxidase activity and organic matter decomposition in waterlogged wetlands. Biogeochemistry 76, 69–83 (2005).
Sinsabaugh, R. L. Phenol oxidase, peroxidase and organic matter dynamics of soil. Soil Biol. Biochem. 42, 391–404 (2010).
Hall, S. J. & Silver, W. L. Iron oxidation stimulates organic matter decomposition in humid tropical forest soils. Glob. Change Biol. 19, 2804–2813 (2013).
Hall, S. J., Treffkorn, J. & Silver, W. L. Breaking the enzymatic latch: impacts of reducing conditions on hydrolytic enzyme activity in tropical forest soils. Ecology 95, 2964–2973 (2014).
Kaiser, K., Mikutta, R. & Guggenberger, G. Increased stability of organic matter sorbed to ferrihydrite and goethite on aging. Soil Sci. Soc. Am. J. 71, 711–719 (2007).
Kaiser, K. & Guggenberger, G. Sorptive stabilization of organic matter by microporous goethite: sorption into small pores vs. surface complexation. Eur. J. Soil Sci. 58, 45–59 (2007).
Riedel, T., Zak, D., Biester, H. & Dittmar, T. Iron traps terrestrially derived dissolved organic matter at redox interfaces. Proc. Natl Acad. Sci. USA 110, 10101–10105 (2013).
Silva, L. C. R. et al. Iron-mediated stabilization of soil carbon amplifies the benefits of ecological restoration in degraded lands. Ecol. Appl. 25, 1226–1234 (2015).
Lalonde, K., Mucci, A., Ouellet, A. & Gélinas, Y. Preservation of organic matter in sediments promoted by iron. Nature 483, 198–200 (2012).
Zhao, Q. et al. Iron-bound organic carbon in forest soils: quantification and characterization. Biogeosciences 13, 4777–4788 (2016).
Mcknight, D. M. et al. Sorption of dissolved organic-carbon by hydrous aluminum and iron-oxides occurring at the confluence of Deer Creek with the Snake River, Summit County, Colorado. Environ. Sci. Technol. 26, 1388–1396 (1992).
Riedel, T., Biester, H. & Dittmar, T. Molecular fractionation of dissolved organic matter with metal salts. Environ. Sci. Technol. 46, 4419–4426 (2012).
Hall, S. J., Silver, W. L., Timokhin, V. I. & Hammel, K. E. Iron addition to soil specifically stabilized lignin. Soil Biol. Biochem. 98, 95–98 (2016).
Kögel-Knabner, I. The macromolecular organic composition of plant and microbial residues as inputs to soil organic matter. Soil Biol. Biochem. 34, 139–162 (2002).
Thevenot, M., Dignac, M. F. & Rumpel, C. Fate of lignins in soils: a review. Soil Biol. Biochem. 42, 1200–1211 (2010).
Hernes, P. J., Kaiser, K., Dyda, R. Y. & Cerli, C. Molecular trickery in soil organic matter: hidden lignin. Environ. Sci. Technol. 47, 9077–9085 (2013).
Hedges, J. I. & Mann, D. The characterization of plant tissues by their lignin oxidation products. Geochim. Cosmochim. Acta 43, 1803–1807 (1979).
Shields, M. R., Bianchi, T. S., Gélinas, Y., Allison, M. A. & Twilley, R. R. Enhanced terrestrial carbon preservation promoted by reactive iron in deltaic sediments. Geophys. Res. Lett. 43, 1149–1157 (2016).
Wang, H., Yu, L., Chen, L., Wang, C. & He, J. Responses of soil respiration to reduced water table and nitrogen addition in an alpine wetland on the Qinghai-Xizang Plateau. Chin. J. Plant Ecol. 38, 619–625 (2014).
Wang, H. et al. Molecular mechanisms of water table lowering and nitrogen deposition in affecting greenhouse gas emissions from a Tibetan alpine wetland. Glob. Change Biol. 23, 815–829 (2017).
Mehra, O. & Jackson, M. in Clays and Clay Minerals (ed. Swinford, A.) 317–327 (Pergamon Press, 1958).
Guggenberger, G. & Kaiser, K. Dissolved organic matter in soil: challenging the paradigm of sorptive preservation. Geoderma 113, 293–310 (2003).
Wagai, R. & Mayer, L. M. Sorptive stabilization of organic matter in soils by hydrous iron oxides. Geochim. Cosmochim. Acta 71, 25–35 (2007).
Spielvogel, S., Prietzel, J. & Kögel-Knabner, I. Changes of lignin phenols and neutral sugars in different soil types of a high-elevation forest ecosystem 25 years after forest dieback. Soil Biol. Biochem. 39, 655–668 (2007).
Opsahl, S. & Benner, R. Characterization of carbohydrates during early diagenesis of five vascular plant tissues. Org. Geochem. 30, 83–94 (1999).
Kleber, M. What is recalcitrant soil organic matter? Environ. Chem. 7, 320–332 (2010).
Davidson, E. A., Samanta, S., Caramori, S. S. & Savage, K. The dual Arrhenius and Michaelis-Menten kinetics model for decomposition of soil organic matter at hourly to seasonal time scales. Glob. Change Biol. 18, 371–384 (2012).
Fenner, N., Freeman, C. & Reynolds, B. Hydrological effects on the diversity of phenolic degrading bacteria in a peatland: implications for carbon cycling. Soil Biol. Biochem. 37, 1277–1287 (2005).
Liu, S. et al. Flooding effects on soil phenol oxidase activity and phenol release during rice straw decomposition. J. Plant Nutr. Soil Sci. 177, 541–547 (2014).
Williams, C. J., Shingara, E. A. & Yavitt, J. B. Phenol oxidase activity in peatlands in New York State: response to summer drought and peat type. Wetlands 20, 416–421 (2000).
Toberman, H. et al. Long-term drainage for forestry inhibits extracellular phenol oxidase activity in Finnish boreal mire peat. Eur. J. Soil Sci. 61, 950–957 (2010).
Toberman, H., Freeman, C., Artz, R. R. E., Evans, C. D. & Fenner, N. Impeded drainage stimulates extracellular phenol oxidase activity in riparian peat cores. Soil Use Manage. 24, 357–365 (2008).
Zsolnay, A. & Gorlitz, H. Water extractable organic matter in arable soils effects of drought and long-term fertilization. Soil Biol. Biochem. 26, 1257–1261 (1994).
Clark, J. M., Chapman, P. J., Heathwaite, A. L. & Adamson, J. K. Suppression of dissolved organic carbon by sulfate induced acidification during simulated droughts. Environ. Sci. Technol. 40, 1776–1783 (2006).
Eshleman, K. N. & Hemond, H. F. The role of organic-acids in the acid-base status of surface waters at Bickford Watershed, Massachusetts. Water Resour. Res. 21, 1503–1510 (1985).
Marschner, B. & Kalbitz, K. Controls of bioavailability and biodegradability of dissolved organic matter in soils. Geoderma 113, 211–235 (2003).
Andersson, S., Nilsson, S. I. & Saetre, P. Leaching of dissolved organic carbon (DOC) and dissolved organic nitrogen (DON) in mor humus as affected by temperature and pH. Soil Biol. Biochem. 32, 1–10 (2000).
Kalbitz, K., Solinger, S., Park, J. H., Michalzik, B. & Matzner, E. Controls on the dynamics of dissolved organic matter in soils: a review. Soil Sci. 165, 277–304 (2000).
Allison, S. D. Soil minerals and humic acids alter enzyme stability: implications for ecosystem processes. Biogeochemistry 81, 361–373 (2006).
Romanowicz, K. J. et al. Understanding drivers of peatland extracellular enzyme activity in the PEATcosm experiment: mixed evidence for enzymic latch hypothesis. Plant Soil 397, 371–386 (2015).
Toberman, H. et al. Summer drought effects upon soil and litter extracellular phenol oxidase activity and soluble carbon release in an upland Calluna heathland. Soil Biol. Biochem. 40, 1519–1532 (2008).
Chen, C., Dynes, J. J., Wang, J. & Sparks, D. L. Properties of Fe-organic matter associations via coprecipitation versus adsorption. Environ. Sci. Technol. 48, 13751–13759 (2014).
Pokrovsky, O. S. et al. Dissolved, suspended, and colloidal fluxes of organic carbon, major and trace elements in the Severnaya Dvina River and its tributary. Chem. Geol. 273, 136–149 (2010).
Badri, D. V. & Vivanco, J. M. Regulation and function of root exudates. Plant Cell Environ. 32, 666–681 (2009).
Schöning, I., Knicker, H. & Kögel-Knabner, I. Intimate association between O/N-alkyl carbon and iron oxides in clay fractions of forest soils. Org. Geochem. 36, 1378–1390 (2005).
Song, W. et al. Methane emissions from an alpine wetland on the Tibetan Plateau: neglected but vital contribution of the nongrowing season. J. Geophys. Res. Biogeosci 120, 1475–1490 (2015).
Harris, D., Horwath, W. R. & van Kessel, C. Acid fumigation of soils to remove carbonates prior to total organic carbon or carbon-13 isotopic analysis. Soil Sci. Soc. Am. J. 65, 1853–1856 (2001).
Saiya-Cork, K. R., Sinsabaugh, R. L. & Zak, D. R. The effects of long term nitrogen deposition on extracellular enzyme activity in an Acer saccharum forest soil. Soil Biol. Biochem. 34, 1309–1315 (2002).
Zimmerman, A. R., Chorover, J., Goyne, K. W. & Brantley, S. L. Protection of mesopore-adsorbed organic matter from enzymatic degradation. Environ. Sci. Technol. 38, 4542–4548 (2004).
Weishaar, J. L. et al. Evaluation of specific ultraviolet absorbance as an indicator of the chemical composition and reactivity of dissolved organic carbon. Environ. Sci. Technol. 37, 4702–4708 (2003).
Stookey, L. L. Ferrozine a new spectrophotometric reagent for iron. Anal. Chem. 42, 779–781 (1970).
Spielvogel, S., Prietzel, J. & Kögel-Knabner, I. Soil organic matter stabilization in acidic forest soils is preferential and soil type-specific. Eur. J. Soil Sci. 59, 674–692 (2008).
Mikutta, R., Kleber, M., Torn, M. S. & Jahn, R. Stabilization of soil organic matter: association with minerals or chemical recalcitrance? Biogeochemistry 77, 25–56 (2006).
Hedges, J. I. & Ertel, J. R. Characterization of lignin by gas capillary chromatography of cupric oxide oxidation products. Anal. Chem. 54, 174–178 (1982).
Otto, A., Shunthirasingham, C. & Simpson, M. J. A comparison of plant and microbial biomarkers in grassland soils from the Prairie Ecozone of Canada. Org. Geochem. 36, 425–448 (2005).
Louchouarn, P., Opsahl, S. & Benner, R. Isolation and quantification of dissolved lignin from natural waters using solid-phase extraction and GC/MS. Anal. Chem. 72, 2780–2787 (2000).
Spencer, R. G. M. et al. Comparison of XAD with other dissolved lignin isolation techniques and a compilation of analytical improvements for the analysis of lignin in aquatic settings. Org. Geochem. 41, 445–453 (2010).
Hedges, J. I., Cowie, G. L., Ertel, J. R., Barbour, R. J. & Hatcher, P. G. Degradation of carbohydrates and lignins in buried woods. Geochim. Cosmochim. Acta 49, 701–711 (1985).
Feng, X. & Simpson, M. J. Temperature responses of individual soil organic matter components. J. Geophys. Res. 113, G03036 (2008).
This study was supported financially by the Chinese National Key Development Program for Basic Research (2015CB954201, 2014CB954003) and the National Natural Science Foundation of China (41422304, 31370491). X.F. acknowledges start-up support from the National Thousand Young Talents recruiting plan of China. Juan Jia is acknowledged for help with sampling. Xinying Zhang, Jingjing Guo, Simin Wang, Zongguang Liu, Shanshan Zhu, Yue Cai and Xin Jing are acknowledged for technical support and help with analyses.
The authors declare no competing financial interests.
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Wang, Y., Wang, H., He, JS. et al. Iron-mediated soil carbon response to water-table decline in an alpine wetland. Nat Commun 8, 15972 (2017). https://doi.org/10.1038/ncomms15972
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