Abstract
Although small molecules diffuse rapidly through the interphase nucleus1,2, recent reports indicate that nuclear diffusion is limited for particles that are larger than 100 nm in diameter3. Given the apparent size limits to nuclear diffusion, there is some debate as to whether the movement of large particles should be attributed to diffusion or to active transport4,5,6. Here, we show that 125 nm-diameter herpes simplex virus 1 (HSV-1) capsids are actively transported within infected nuclei. Movement is directed, temperature- and energy-dependent, sensitive to the putative myosin inhibitor 2,3-butanedione monoxime (BDM)7,8 and to actin depolymerization with latrunculin-A, but insensitive to actin depolymerization with cytochalasin-D.
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Acknowledgements
We thank P. Desai for the gift of HSV-1 strain K26GFP, B. Baird for discussions concerning mathematical treatment of the kinetic data, and W. Federer for statistical advice. This research was supported by a National Institutes of Health grant (R01 GM50740) to J.D.B.; a training grant (T35 AI07227-19) to Cornell University for support of S.B.; and Merck & Co for support of T.F.
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Forest, T., Barnard, S. & Baines, J. Active intranuclear movement of herpesvirus capsids. Nat Cell Biol 7, 429–431 (2005). https://doi.org/10.1038/ncb1243
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DOI: https://doi.org/10.1038/ncb1243
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