Rat smooth muscle cell that has been transfected with green fluorescent protein (GFP)-tagged α-actinin (green) and stained for filamentous actin and vinculin. The actin cytoskeleton was stained with Alexa-568-conjugated phalloidin (red) and vinculin immunostaining is shown in blue. The image was acquired using a fluorescence microscope (Axioskop, Zeiss). Scale bar represents 20 μm.