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Evidence that RME-1, a conserved C. elegans EH-domain protein, functions in endocytic recycling

Nature Cell Biology volume 3, pages 573–579 (2001)Cite this article

Abstract

In genetic screens for new endocytosis genes in Caenorhabditis elegans we identified RME-1, a member of a conserved class of Eps15-homology (EH)-domain proteins. Here we show that RME-1 is associated with the periphery of endocytic organelles, which is consistent with a direct role in endocytic transport. Endocytic defects in rme-1 mutants indicate that the protein is likely to have a function in endocytic recycling. Evidence from studies of mammalian RME-1 also points to a function for RME-1 in recycling, specifically in the exit of membrane proteins from recycling endosomes. These studies show a conserved function in endocytic recycling for the RME-1 family of EH proteins.

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Figure 1: rme-1 mutant phenotypes.
Figure 2: Gene structure and protein product of rme-1.
Figure 3: Subcellular localization of RME-1 in wild-type and mutant strains.
Figure 4: Intestinal cells of rme-1 mutants are defective in transport of basolateral fluid-phase markers.

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Acknowledgements

We thank L. Pedraza and W. Pryzlecki for technical assistance; H. Fares, V. Irikura, Y. Kohara, X. Li and A. Melendez for reagents and strains; and Q. Al-Aqwati, H. Fares, I. Greenwald, F. Maxfield and H. Wilkinson for discussions during this work and for comments on this manuscript. We also thank H. Fares and I. Greenwald for the gift of rme-1 mutant strains. Many of the strains used in this work were provided by the Caenorhabditis Genetics Center. This work was supported by a grant from NIH to D.H.H., by a NIH National Service Research Award to B.G. and by a March of Dimes Grant to D.H.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biophysics, Columbia University College of Physicians and Surgeons, 630 West 168th Street, New York, 10032, New York, USA

    Barth Grant, Yinhua Zhang & David Hirsh

  2. Department of Neuroscience, Center for C. elegans Anatomy, Albert Einstein College of Medicine, 1410 Pelham Parkway, Bronx, 10461, New York, USA

    Marie-Christine Paupard & David H. Hall

  3. Department of Biochemistry, Weill Medical College of Cornell University, New York, 10021, New York, USA

    Sharron X. Lin

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Correspondence to Barth Grant.

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Grant, B., Zhang, Y., Paupard, MC. et al. Evidence that RME-1, a conserved C. elegans EH-domain protein, functions in endocytic recycling. Nat Cell Biol 3, 573–579 (2001). https://doi.org/10.1038/35078549

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