Lipid rafts are thought to be important for signalling cascades by compartmentalizing signalling components in the membrane. This view has recently come under fire because the common method used for isolation of rafts — detergent extraction — is fraught with artefacts. A variety of techniques, including FRET, have been used recently to investigate rafts in their native environment in intact cells. Now, FRET analysis provides evidence that rafts may not have a role in T-cell activation after all.
This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
-
Ultrastructure and lipid composition of detergent-resistant membranes derived from mammalian sperm and two types of epithelial cells
Cell and Tissue Research Open Access 16 September 2015
Access options
Subscribe to this journal
Receive 12 print issues and online access
$209.00 per year
only $17.42 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Harder, T. Adv. Immunol. 77, 45–92 (2001).
Germain, R. J. Biol. Chem. 276, 35223–35226 (2001).
Munro, S. Cell 115, 377–288 (2003).
Glebov, O.O. & Nichols, B.J. Nature Cell Biol. 6, 238–243 (2004).
Dykstra, M.L., Cherukuri, A., Sohn, H.W., Tzeng, S.-J. & Pierce, S.K. Ann. Rev. Immunol. 21, 457–481 (2003).
Sharma, P. et al. Cell (in the press).
Ediden, M. Annu. Rev. Biophys. Biomol.Struct. 32, 257–283 (2003).
Dustin, M.L. J. Clin. Invest. 109, 155–160 (2002).
Viola, A., Schroeder, S., Sakakibara, Y. & Lanzavecchia, A. Science 283, 680–682 (1999).
Burack, W.R., Lee, K.H., Holdorf, A.D., Dustin, M.L. & Shaw, A.S. J. Immunol. 169, 2837–2841 (2002).
Harder, T. & Kuhn, M. J. Cell Biol. 151, 199–207 (2000).
Bunnell, S.C. et al. J. Cell Biol. 158, 1263–1275 (2002).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pierce, S. To cluster or not to cluster: FRETting over rafts. Nat Cell Biol 6, 180–181 (2004). https://doi.org/10.1038/ncb0304-180
Issue Date:
DOI: https://doi.org/10.1038/ncb0304-180
This article is cited by
-
Ultrastructure and lipid composition of detergent-resistant membranes derived from mammalian sperm and two types of epithelial cells
Cell and Tissue Research (2016)
-
Oligodendroglial Cells and Neurotrophins: A Polyphonic Cantata in Major and Minor
Journal of Molecular Neuroscience (2008)
-
Lipid rafts: now you see them, now you don't
Nature Immunology (2006)
-
Lipid rafts in plants
Planta (2005)
-
Molecular interdiction of Src-family kinase signaling in hematopoietic cells
Oncogene (2004)