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A Bifunctional Affinity Linker to Couple Antibodies to Cellulose

Bio/Technology volume 11, pages 15701573 (1993) | Download Citation

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Abstract

We have constructed a fusion protein between the staphylococcal A protein and the cellulose binding domain of an exoglucanase (Cex) from Cellulomonas fimi that can be directly immobilized on cellulose while retaining its capacity to bind immunoglobulin G molecules. The cellulose domain provides binding that does not interfere with the biological activity of the fusion partner, does not involve hazardous chemicals and the matrix does not need to be chemically activated which reduces its cost. We have tested some of the possible applications of the fusion protein and show that it can be used in immunoassays, affinity chromatography and immunoprecipitations.

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Author notes

    • Douglas G. Kilburn

    Corresponding author.

Affiliations

  1. Department of Microbiology and Protein Engineering Network of Centres of Excellence, University of British Columbia, ♯300–6147 University Blvd. Vancouver, B.C., Canada V6T 1Z3.

    • Celia Ramírez
    • , Julian Fung
    • , Robert C. Miller Jr.
    • , R. Antony
    • , J. Warren
    •  & Douglas G. Kilburn

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DOI

https://doi.org/10.1038/nbt1293-1570

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