Abstract
Escherichia coli, having high aspartase activity, were immobilized by covalent attachment and entrapment with polyazetidine with .retention of 97% of the free cell activity. The projected enzyme half-life was 611 days. Stability of the system was greater than 2 years at 25°C without loss of activity when stored in 1.5 M ammonium aspartate. The system provides attractive benefits for the commercial production of specialty chemicals.
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Wood, L., Calton, G. A Novel Method of Immobilization and its Use in Aspartic Acid Production. Nat Biotechnol 2, 1081–1084 (1984). https://doi.org/10.1038/nbt1284-1081
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DOI: https://doi.org/10.1038/nbt1284-1081
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