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Transgenic potato expressing a double-stranded RNA-specific ribonuclease is resistant to potato spindle tuber viroid

Nature Biotechnology volume 15, pages 1290–1294 (1997)Cite this article

Abstract

We have produced transgenic potato lines expressing the yeast-derived double-stranded RNA-specific ribonuclease pac1. Five lines of pac1 potato (Solanum tuberosum L, cultivar Russet Burbank) challenged with potato spindle tuber viroid (PSTVd) suppressed PSTVd infection and accumulation. All of the progeny potato tubers produced by resistant plants were also free of PSTVd. Because the pac1 gene product digested PSTVd in vitro, double-stranded regions in PSTVd molecule and/or replicative intermediates may be targeted by pac1 gene product in the transgenic potato plant.

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Authors and Affiliations

  1. Laboratory of Phytopathology, Faculty of Agriculture, Hirosaki University, Hirosaki, 036, Japan

    Teruo Sano & Akihiro Nagayama

  2. Central Laboratories for Key Technology, KIRIN Brewery Co. Ltd., 1-13-5 Fukuura, Kanazawa-ku, Yokohama, 236, Japan

    Toshiya Ogawa & Isao Ishida

  3. Department of Biosciences, School of Science and Engineering, Teikyo University, Toyosato-dai 1-1, Utsunomiya, 320, Japan

    Yoshimi Okada

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  1. Teruo Sano
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  4. Isao Ishida
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  5. Yoshimi Okada
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Sano, T., Nagayama, A., Ogawa, T. et al. Transgenic potato expressing a double-stranded RNA-specific ribonuclease is resistant to potato spindle tuber viroid. Nat Biotechnol 15, 1290–1294 (1997). https://doi.org/10.1038/nbt1197-1290

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  • DOI: https://doi.org/10.1038/nbt1197-1290

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