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Stabilization of Escherichia coli Tryptophan–Production Vectors in Continuous Cultures: A Comparison of Three Different Systems

Bio/Technology volume 4, pages 901–903 (1986)Cite this article

Abstract

The valS-system for plasmid stabilization involves cloning the wild–type gene for valyl–tRNA synthetase (valS) in a plasmid which is transformed into a host strain carrying a temperature–sensitive mutation in its chromosomal valS–gene. At the non–permissive temperature cell growth is de pendent on the plasmid–encoded wild–type enzyme. Partition (par) loci from R1 or pSC101 plasmids did not stabilize plasmid inheritance. Although both par loci stabilized segregation somewhat, by 75–100 generations plasmids were lost from the majority of the cells. In contrast, in heritance of the plasmid was stabilized with the valS system for at least 150 generations.

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Author notes

  1. S. Gunnar Skogman

    Present address: SYN-TEK AB, Box 1010, S-901 20, Umeå, Sweden

Authors and Affiliations

  1. AC Biotechnics AB, Box 946, S-220 09, Lund, Sweden

    Jörgen Nilsson & S. Gunnar Skogman

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  1. Jörgen Nilsson
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Nilsson, J., Skogman, S. Stabilization of Escherichia coli Tryptophan–Production Vectors in Continuous Cultures: A Comparison of Three Different Systems. Nat Biotechnol 4, 901–903 (1986). https://doi.org/10.1038/nbt1086-901

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