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Automated DNA Sequencing: Progress and Prospects

Bio/Technology volume 4, pages 890–895 (1986)Cite this article

Abstract

In the decade since their inception, manual DNA sequencing techniques have made only minor inroads into the primary biological data–base. Attempts have been made to automate the process in order to achieve an enhanced rate of data–capture to allow access to the larger genome. Progress in instrumenting these techniques has been restricted because of their complexity and the multidisciplinary nature of the procedures involved. This article outlines the manual methods presently employed and reviews current sequencing methodology. A rationale for the possible successful automation of DNA sequencing incorporating a potential for improvements in related areas is presented. Recent sequence instrumentation projects are assessed and an account is provided of contemporary national and international proposals to implement accelerated DNA sequence data–capture.

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Author notes

  1. William J. Martin: Department of Instrumentation and Analytical Science, University of Manchester Institute of Science and Technology, P.O. Box 88, Manchester M60 1QD, U.K.

  2. R. Wayne Davies: Allelix Inc., 6850 Goreway Drive, Mississauga, Ontario L4V 1P1, Canada.

  3. William J. Martin and R. Wayne Davies: Author to whom correspondence should be addressed.

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  1. William J. Martin
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Martin, W., Davies, R. Automated DNA Sequencing: Progress and Prospects. Nat Biotechnol 4, 890–895 (1986). https://doi.org/10.1038/nbt1086-890

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