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Stable Transformation of Soybean (Glycine Max) by Particle Acceleration

Bio/Technology volume 6, pages 923–926 (1988)Cite this article

Abstract

We have used particle acceleration by electric discharge to introduce DNA-coated gold particles into meristems of immature soybean seeds. Approximately 2 percent of shoots derived from these meristems via organogenesis were chimeric for expression of the introduced gene. Experiments using this protocol have resulted in mature RO and R1 plants expressing the introduced gene(s). We regard this work as a major step in the practical application of genetic engineering to the useful modification of food-crop species.

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Author notes

  1. Paul Christou: Corresponding author.

Authors and Affiliations

  1. Agracetus, 8520 University Green, Middleton, WI, 53562

    Dennis E. McCabe, William F. Swain, Brian J. Martinell & Paul Christou

Authors
  1. Dennis E. McCabe
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  2. William F. Swain
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  3. Brian J. Martinell
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  4. Paul Christou
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McCabe, D., Swain, W., Martinell, B. et al. Stable Transformation of Soybean (Glycine Max) by Particle Acceleration. Nat Biotechnol 6, 923–926 (1988). https://doi.org/10.1038/nbt0888-923

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  • DOI: https://doi.org/10.1038/nbt0888-923

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