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Bacterial Dihydrodipicolinate Synthase and Desensitized Aspartate Kinase: Two Novel Selectable Markers for Plant Transformation

Bio/Technology volume 11, pages 715–718 (1993)Cite this article

Abstract

Potato plants transformed with a chimeric gene encoding a bacterial desensitized aspartate kinase were selected for resistance to the presence of lysine plus threonine in the regeneration and rooting media. Similarly, plants transformed with a chimeric gene encoding a bacterial dihydrodipicolinate synthase were selected for resistance to the toxic lysine analog S-aminoethyl L-cysteine. In both cases, resistant plants were regenerated, and all were apparently transgenic based on their content of dihydrodipicolinate synthase or aspartate kinase activities that were significantly higher, and much less sensitive to lysine and threonine inhibition, than the endogenous activities in control untransformed plants. Our data suggest that these novel selectable markers may be useful for the isolation of transgenic plants expressing relatively high levels of the gene of interest.

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Author notes

  1. Avihai Perl

    Present address: Department of Fruit Tree Breeding and Genetics, The Volcani Center, Bet-Dagan, 50250, Israel

  2. Gad Galili: Corresponding author.

Authors and Affiliations

  1. Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel

    Shmuel Galili, Orit Shaul, Inbal Ben-Tzvi & Gad Galili

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  1. Avihai Perl
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  2. Shmuel Galili
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Perl, A., Galili, S., Shaul, O. et al. Bacterial Dihydrodipicolinate Synthase and Desensitized Aspartate Kinase: Two Novel Selectable Markers for Plant Transformation. Nat Biotechnol 11, 715–718 (1993). https://doi.org/10.1038/nbt0693-715

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